TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.160
(878 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN820761.1|CN820761 HRO4414_D12_G24ZS5 Lib AA069E1X Aven... 241 2e-064
gb|CN815951.1|CN815951 HRO4515_C06_F11ZS5 Lib AA071E1X Aven... 190 3e-049
>gb|CN820761.1|CN820761 HRO4414_D12_G24ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4414_D12_G24, mRNA
sequence
Length = 649
Score = 241 bits (520), Expect = 2e-064
Identities = 100/116 (86%), Positives = 111/116 (95%)
Frame = -3 / +3
Query: 726 VRRASEAKCLKRGVKEVVKSIRRGNKGLCVIAGNISPIDVITHVPILCEEANVPYVYVPS 547
VRRASEAKCLKRGVKEVVKSIRRGNKGLCVIAGNISPIDVITH+PILCEEANVPY+YV S
Sbjct: 12 VRRASEAKCLKRGVKEVVKSIRRGNKGLCVIAGNISPIDVITHLPILCEEANVPYIYVHS 191
Query: 546 KEDLATAGTTKRPTCCVLVLTKPNKGELSGEVKDKLKSDYDQVVTEVAEATSAMF* 379
KE+LATAGTTKRPTCCVLV+TKP KGE+S E+K+KL+ +Y+QVVTEVAE TSAM+*
Sbjct: 192 KEELATAGTTKRPTCCVLVMTKPAKGEISEELKEKLQKEYNQVVTEVAEVTSAMY* 359
Score = 164 bits (352), Expect(2) = 6e-043
Identities = 71/83 (85%), Positives = 74/83 (89%)
Frame = +2 / -1
Query: 479 GLVRTKTQQVGLLVVPAVARSSFDGTYT*GTLASSHSIGTCVITSIGEIFPAITHNPLFP 658
G V T TQQVGLLVVPAVA SSF+ TY *GTLASSHS+G CVITSIGE+FPAITHNPLFP
Sbjct: 259 GFVMTSTQQVGLLVVPAVASSSFECTYI*GTLASSHSMGRCVITSIGEMFPAITHNPLFP 80
Query: 659 RRMLLTTSLTPRLRHFASEARRT 727
RRMLLTTSLTPR RH ASEARRT
Sbjct: 79 RRMLLTTSLTPRFRHLASEARRT 11
Score = 157 bits (338), Expect = 2e-039
Identities = 66/116 (56%), Positives = 86/116 (74%)
Frame = +1 / -3
Query: 379 SEHCRCSLSDFRYNLVIVRLQFILHLSTELPLVWFGQNQDATSRPLSGSCGRKILF*WNI 558
S HCRC+LS+F YNLV+ LQF+L L T+LPL W +Q A SRPL K+LF* +I
Sbjct: 359 SVHCRCNLSNFSYNLVVFLLQFLLQLFTDLPLGWLRHDQYAASRPLGSPSSSKLLF*VHI 180
Query: 559 YVRNISFLT*YRNMCNHINRRNISSDHTQSFVPAANALDYLFDSALEALRLGGSSH 726
Y+RNISFLT*Y MCNHI+RR++SS++ Q FVP +AL++L D AL+AL L S++
Sbjct: 179 YIRNISFLT*YGKMCNHIDRRDVSSNYAQPFVPTTDALNHLLDPALQALGLRSSAN 12
Score = 148 bits (318), Expect = 1e-036
Identities = 69/118 (58%), Positives = 82/118 (69%)
Frame = -2 / +2
Query: 727 RATSLRGEVPQARSQRGSQEHSPREQRIVCDRWKYFSY*CDYTCSDTM*GS*CSLRICSI 548
R+ S G+VP+AR Q G EH EQR+V + WK+ SY CDYT S TM*GS*CSL IC++
Sbjct: 11 RSPSFGGQVPEARGQGGG*EHPSWEQRVVRNCWKHLSYRCDYTSSHTM*GS*CSLYICAL 190
Query: 547 KRGSCDRRNH*EADLLRLGSDQTKQGGAQWRGEG*TEVGL*PSCNGSR*GYICNVLRT 374
KRG+C ++ EA LLR G D+ QGG Q R EG T G+ PSCN S *GYICNVL T
Sbjct: 191 KRGACYCWDYQEAYLLRTGHDEASQGGDQ*RVEGETAEGIQPSCN*SC*GYICNVLIT 364
Score = 101 bits (216), Expect = 1e-022
Identities = 57/102 (55%), Positives = 66/102 (64%)
Frame = +3 / -2
Query: 423 GHSPTSVYPSPLH*APPCLVWSEPRRNKSAS*WFLRSQDPLLMEHIRKEH*LPHIVSEHV 602
G P++V PS LH*+PP L S P R+K AS + Q PLL HI KEH*LPHIV E V
Sbjct: 315 GCIPSAVSPSTLH*SPPWLASS*PVRSK*ASW*SQQ*QAPLLSAHIYKEH*LPHIVWEDV 136
Query: 603 *SHQ*EKYFQRSHTILCSRGECS*LPL*LRA*GTSPRRLVAR 728
*SH+*E+ FQ+ T LCS CS* P * RA GT P +L R
Sbjct: 135 *SHR*ERCFQQLRTTLCSHDGCS*PPP*PRASGTWPPKLGER 10
Score = 95.9 bits (203), Expect = 8e-021
Identities = 52/94 (55%), Positives = 58/94 (61%)
Frame = -1 / +1
Query: 656 GTKDCV*SLEIFXXXXXLHMFRYYVRKLMFLTYMFHQKRILRPQEPLRGRLVASWF*PNQ 477
GTK C * LE LH+F YYVRKLMFL YM QKR L RG L A W * +Q
Sbjct: 82 GTKGCA*LLETSLLSM*LHIFPYYVRKLMFLIYMCTQKRSLLLLGLPRGLLAAYWS*RSQ 261
Query: 476 TRGSSVER*RIN*SRTMTKL*RKSLRLHLQCSED 375
RG SV+ *R N R TKL* K LRLHLQC+++
Sbjct: 262 PRGRSVKS*RRNCRRNTTKL*LKLLRLHLQCTDN 363
Score = 25.4 bits (49), Expect(2) = 6e-043
Identities = 8/12 (66%), Positives = 10/12 (83%)
Frame = +1 / -1
Query: 325 YTKKDVHRELPD 360
Y +KDVH E+PD
Sbjct: 421 YRRKDVHSEVPD 386
>gb|CN815951.1|CN815951 HRO4515_C06_F11ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_C06_F11, mRNA sequence
Length = 433
Score = 190 bits (409), Expect = 3e-049
Identities = 77/94 (81%), Positives = 88/94 (93%)
Frame = -3 / +1
Query: 660 RGNKGLCVIAGNISPIDVITHVPILCEEANVPYVYVPSKEDLATAGTTKRPTCCVLVLTK 481
RG KGLCVIAGNISPIDVITH+PILCEEANVPY+YV SKE+LATAGTTKRPTCCVLV+TK
Sbjct: 10 RGEKGLCVIAGNISPIDVITHLPILCEEANVPYIYVHSKEELATAGTTKRPTCCVLVMTK 189
Query: 480 PNKGELSGEVKDKLKSDYDQVVTEVAEATSAMF* 379
P KGE+S E+K+KL+ +Y+QVVTEVAE TSAM+*
Sbjct: 190 PAKGEISEELKEKLQKEYNQVVTEVAEVTSAMY* 291
Score = 135 bits (289), Expect = 1e-032
Identities = 55/94 (58%), Positives = 70/94 (74%)
Frame = +1 / -2
Query: 379 SEHCRCSLSDFRYNLVIVRLQFILHLSTELPLVWFGQNQDATSRPLSGSCGRKILF*WNI 558
S HCRC+LS+F YNLV+ LQF+L L T+LPL W +Q A SRPL K+LF* +I
Sbjct: 291 SVHCRCNLSNFSYNLVVFLLQFLLQLFTDLPLGWLRHDQYAASRPLGSPSSSKLLF*VHI 112
Query: 559 YVRNISFLT*YRNMCNHINRRNISSDHTQSFVPA 660
Y+RNISFLT*Y MCNHI+RR++SS++ Q F P+
Sbjct: 111 YIRNISFLT*YGKMCNHIDRRDVSSNYAQPFFPS 10
Score = 122 bits (262), Expect = 6e-029
Identities = 57/95 (60%), Positives = 68/95 (71%)
Frame = -2 / +3
Query: 658 REQRIVCDRWKYFSY*CDYTCSDTM*GS*CSLRICSIKRGSCDRRNH*EADLLRLGSDQT 479
R +R+V + WK+ SY CDYT S TM*GS*CSL IC++KRG+C ++ EA LLR G D+
Sbjct: 12 RGKRVVRNCWKHLSYRCDYTSSHTM*GS*CSLYICALKRGACYCWDYQEAYLLRTGHDEA 191
Query: 478 KQGGAQWRGEG*TEVGL*PSCNGSR*GYICNVLRT 374
QGG Q R EG T G+ PSCN S *GYICNVL T
Sbjct: 192 SQGGDQ*RVEGETAEGIQPSCN*SC*GYICNVLIT 296
Score = 113 bits (242), Expect = 3e-026
Identities = 49/61 (80%), Positives = 52/61 (85%)
Frame = +2 / -3
Query: 479 GLVRTKTQQVGLLVVPAVARSSFDGTYT*GTLASSHSIGTCVITSIGEIFPAITHNPLFP 658
G V T TQQVGLLVVPAVA SSF+ TY *GTLASSHS+G CVITSIGE+FPAITHNP P
Sbjct: 191 GFVMTSTQQVGLLVVPAVASSSFECTYI*GTLASSHSMGRCVITSIGEMFPAITHNPFSP 12
Query: 659 R 661
R
Sbjct: 11 R 9
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 10,487,852
Number of Sequences: 8143
Number of extensions: 163671
Number of successful extensions: 1847
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 303117162
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)