TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.134
(674 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN821624.1|CN821624 HRO4408_D06_H12ZS5 Lib AA069E1X Aven... 193 3e-050
gb|CN815219.1|CN815219 HRO4507_C01_F01ZS5 Lib AA071E1X Aven... 183 2e-047
gb|CN815929.1|CN815929 HRO4515_A08_B15ZS5 Lib AA071E1X Aven... 183 2e-047
gb|CN817060.1|CN817060 HRO4527_G09_N17ZS5 Lib AA071E1X Aven... 183 2e-047
gb|CN820741.1|CN820741 HRO4414_B07_C14ZS5 Lib AA069E1X Aven... 182 7e-047
gb|CN816090.1|CN816090 HRO4516_H02_P04ZS5 Lib AA071E1X Aven... 180 2e-046
gb|CN817108.1|CN817108 HRO4528_C10_F20ZS5 Lib AA071E1X Aven... 141 1e-034
gb|CN815430.1|CN815430 HRO4509_F01_K01ZS5 Lib AA071E1X Aven... 100 2e-022
>gb|CN821624.1|CN821624 HRO4408_D06_H12ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4408_D06_H12, mRNA
sequence
Length = 415
Score = 193 bits (415), Expect = 3e-050
Identities = 90/108 (83%), Positives = 91/108 (84%)
Frame = -2 / +2
Query: 439 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSR 260
SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF ARYNKKPTITSR
Sbjct: 38 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSR 217
Query: 259 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*GAAARCYDPV*FSYL 116
EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* AA + D V FS L
Sbjct: 218 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*AAAQQLVD*VAFSCL 361
Score = 88.1 bits (186), Expect(2) = 2e-031
Identities = 43/54 (79%), Positives = 44/54 (81%)
Frame = +3 / -2
Query: 117 KYEN*TGS*QRAAAP*EDVNLVTALVPSDTACLASSPGRTRRTEVWISREVMVG 278
K E T S AA *EDVNLVTALVPS+TACLASSPGRTRRTEV ISREVMVG
Sbjct: 360 KQEKAT*STSCCAAA*EDVNLVTALVPSETACLASSPGRTRRTEVCISREVMVG 199
Score = 62.5 bits (130), Expect(2) = 2e-031
Identities = 25/27 (92%), Positives = 27/27 (100%)
Frame = +3 / -2
Query: 345 EFMMDMALDEMPMSGCTCLSTLKM*IL 425
EFMMDMAL++MPMSGCTCLSTLKM*IL
Sbjct: 132 EFMMDMALEDMPMSGCTCLSTLKM*IL 52
>gb|CN815219.1|CN815219 HRO4507_C01_F01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4507_C01_F01, mRNA sequence
Length = 365
Score = 183 bits (395), Expect = 2e-047
Identities = 83/93 (89%), Positives = 83/93 (89%)
Frame = -2 / +3
Query: 439 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSR 260
SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF ARYNKKPTITSR
Sbjct: 66 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSR 245
Query: 259 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 161
EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 246 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 344
Score = 87.7 bits (185), Expect(2) = 5e-031
Identities = 37/39 (94%), Positives = 38/39 (97%)
Frame = +3 / -2
Query: 162 *EDVNLVTALVPSDTACLASSPGRTRRTEVWISREVMVG 278
*EDVN VTALVPS+TACLASSPGRTRRTEVWISREVMVG
Sbjct: 343 *EDVNFVTALVPSETACLASSPGRTRRTEVWISREVMVG 227
Score = 61.6 bits (128), Expect(2) = 5e-031
Identities = 25/27 (92%), Positives = 26/27 (96%)
Frame = +3 / -2
Query: 345 EFMMDMALDEMPMSGCTCLSTLKM*IL 425
EFMMDMAL+EMPMSGCTCL TLKM*IL
Sbjct: 160 EFMMDMALEEMPMSGCTCLRTLKM*IL 80
>gb|CN815929.1|CN815929 HRO4515_A08_B15ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_A08_B15, mRNA sequence
Length = 655
Score = 183 bits (395), Expect = 2e-047
Identities = 83/93 (89%), Positives = 83/93 (89%)
Frame = -2 / +3
Query: 439 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSR 260
SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF ARYNKKPTITSR
Sbjct: 78 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSR 257
Query: 259 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 161
EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 258 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 356
Score = 142 bits (305), Expect = 5e-035
Identities = 63/95 (66%), Positives = 66/95 (69%)
Frame = +3 / -1
Query: 147 RAAAP*EDVNLVTALVPSDTACLASSPGRTRRTEVWISREVMVGXXXXXXXXXXXXXXXX 326
R *++VN VTALVPSDTACLASSPGRTRRTEVWISREVMVG
Sbjct: 370 RLGTD*DEVNFVTALVPSDTACLASSPGRTRRTEVWISREVMVGFLLYLASFADSPASFS 191
Query: 327 XXXXXXEFMMDMALDEMPMSGCTCLSTLKM*ILYV 431
EFMMDMAL+EMPMSGCTC TLKM*ILYV
Sbjct: 190 KMSLMKEFMMDMALEEMPMSGCTCFRTLKM*ILYV 86
>gb|CN817060.1|CN817060 HRO4527_G09_N17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4527_G09_N17, mRNA sequence
Length = 412
Score = 183 bits (395), Expect = 2e-047
Identities = 83/93 (89%), Positives = 83/93 (89%)
Frame = -2 / +1
Query: 439 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSR 260
SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF ARYNKKPTITSR
Sbjct: 25 SVETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSR 204
Query: 259 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 161
EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 205 EIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 303
Score = 87.7 bits (185), Expect(2) = 3e-031
Identities = 36/38 (94%), Positives = 38/38 (100%)
Frame = +3 / -3
Query: 165 EDVNLVTALVPSDTACLASSPGRTRRTEVWISREVMVG 278
++VNLVTALVPSDTACLASSPGRTRRTEVWISREVMVG
Sbjct: 299 DEVNLVTALVPSDTACLASSPGRTRRTEVWISREVMVG 186
Score = 62.0 bits (129), Expect(2) = 3e-031
Identities = 25/27 (92%), Positives = 26/27 (96%)
Frame = +3 / -3
Query: 345 EFMMDMALDEMPMSGCTCLSTLKM*IL 425
EFMMDMAL+EMPMSGCTC STLKM*IL
Sbjct: 119 EFMMDMALEEMPMSGCTCFSTLKM*IL 39
>gb|CN820741.1|CN820741 HRO4414_B07_C14ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4414_B07_C14, mRNA
sequence
Length = 454
Score = 182 bits (391), Expect = 7e-047
Identities = 82/92 (89%), Positives = 82/92 (89%)
Frame = -2 / +1
Query: 436 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSRE 257
VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF ARYNKKPTITSRE
Sbjct: 13 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSRE 192
Query: 256 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 161
IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 193 IQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 288
Score = 87.7 bits (185), Expect(2) = 5e-031
Identities = 37/39 (94%), Positives = 38/39 (97%)
Frame = +3 / -3
Query: 162 *EDVNLVTALVPSDTACLASSPGRTRRTEVWISREVMVG 278
*EDVN VTALVPS+TACLASSPGRTRRTEVWISREVMVG
Sbjct: 287 *EDVNFVTALVPSETACLASSPGRTRRTEVWISREVMVG 171
Score = 61.6 bits (128), Expect(2) = 5e-031
Identities = 25/27 (92%), Positives = 26/27 (96%)
Frame = +3 / -3
Query: 345 EFMMDMALDEMPMSGCTCLSTLKM*IL 425
EFMMDMAL+EMPMSGCTCL TLKM*IL
Sbjct: 104 EFMMDMALEEMPMSGCTCLRTLKM*IL 24
>gb|CN816090.1|CN816090 HRO4516_H02_P04ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4516_H02_P04, mRNA sequence
Length = 472
Score = 180 bits (387), Expect = 2e-046
Identities = 81/91 (89%), Positives = 81/91 (89%)
Frame = -2 / +2
Query: 433 ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSREI 254
ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIF ARYNKKPTITSREI
Sbjct: 11 ETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGESAKLARYNKKPTITSREI 190
Query: 253 QTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 161
QTSVRLVLPGELAKHAVSEGTKAVTKFTSS*
Sbjct: 191 QTSVRLVLPGELAKHAVSEGTKAVTKFTSS* 283
Score = 128 bits (274), Expect = 1e-030
Identities = 59/88 (67%), Positives = 62/88 (70%)
Frame = +3 / -2
Query: 162 *EDVNLVTALVPSDTACLASSPGRTRRTEVWISREVMVGXXXXXXXXXXXXXXXXXXXXX 341
*E+VNLVTALVPS+TACLASSPG TRRTEVWIS EVMVG
Sbjct: 282 *EEVNLVTALVPSETACLASSPGSTRRTEVWISLEVMVGFLLYLASLADSPASFSKISLM 103
Query: 342 XEFMMDMALDEMPMSGCTCLSTLKM*IL 425
EFMMDMAL+EMPMSG TCL TLKM*IL
Sbjct: 102 KEFMMDMALEEMPMSGWTCLRTLKM*IL 19
>gb|CN817108.1|CN817108 HRO4528_C10_F20ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4528_C10_F20, mRNA sequence
Length = 360
Score = 141 bits (303), Expect = 1e-034
Identities = 69/88 (78%), Positives = 70/88 (79%)
Frame = -2 / +2
Query: 379 GISSKAMSIMNSFINDIFXXXXXXXXXXARYNKKPTITSREIQTSVRLVLPGELAKHAVS 200
GISS AMSIMNSFINDIF ARYNKKPTITSREIQTSVRLVLPGELAKHAVS
Sbjct: 11 GISS*AMSIMNSFINDIFEKLAGESAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVS 190
Query: 199 EGTKAVTKFTSS*GAAARCYDPV*FSYL 116
EGTKAVTKFTSS* AA + D V FS L
Sbjct: 191 EGTKAVTKFTSS*AAAQQLVD*VAFSCL 274
>gb|CN815430.1|CN815430 HRO4509_F01_K01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4509_F01_K01, mRNA sequence
Length = 361
Score = 100 bits (214), Expect = 2e-022
Identities = 45/46 (97%), Positives = 45/46 (97%)
Frame = -2 / +3
Query: 292 RYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*GA 155
RYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS* A
Sbjct: 45 RYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTKFTSS*TA 182
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 4,572,140
Number of Sequences: 8143
Number of extensions: 43754
Number of successful extensions: 671
Number of sequences better than 1.0e-020: 8
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 223037542
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)