TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161285.2.2
(798 letters)
Database: At_nucl_with_EST.fasta
1,013,581 sequences; 908,940,872 total letters
Score E
Sequences producing significant alignments: (bits) Value
ref|NM_111911.3| Arabidopsis thaliana ASD1; hydrolase, acti... 77 2e-033
gb|AY243509.1| Arabidopsis thaliana alpha-L-arabinofuranosi... 77 2e-033
gb|AF372949.1|AF372949 Arabidopsis thaliana AT3g10740/T7M13... 77 2e-033
gb|AY143944.1| Arabidopsis thaliana At3g10740/T7M13_18 mRNA... 77 2e-033
dbj|AK222175.1| Arabidopsis thaliana mRNA for putative alph... 77 2e-033
dbj|AK220766.1| Arabidopsis thaliana mRNA for putative alph... 77 2e-033
gb|BX836814.1|BX836814 BX836814 Arabidopsis thaliana Flower... 77 7e-032
emb|BX824011.1|CNS0A4VB Arabidopsis thaliana Full-length cD... 68 3e-029
gb|AY063925.1| Arabidopsis thaliana putative arabinosidase ... 63 3e-023
ref|NM_122513.3| Arabidopsis thaliana hydrolase, acting on ... 63 3e-023
gb|BT006062.1| Arabidopsis thaliana clone U18147 unknown pr... 63 3e-023
gb|AY243510.1| Arabidopsis thaliana alpha-L-arabinofuranosi... 63 3e-023
>ref|NM_111911.3| Arabidopsis thaliana ASD1; hydrolase, acting on glycosyl bonds
AT3G10740 (ASD1) mRNA, complete cds
Length = 2427
Score = 77.1 bits (162), Expect(3) = 2e-033
Identities = 35/59 (59%), Positives = 39/59 (66%)
Frame = +1 / +2
Query: 298 GSTATVLTSGNVMDENSFSNPNKVAPVKSQLSNAAEQMQVTLAPHSFTAFDLALAEPKL 474
GS TVLTS NVMDENSFS P KV P +S L A E M V L PHSF++FDL K+
Sbjct: 2057 GSKKTVLTSTNVMDENSFSQPEKVVPHESLLELAEEDMTVVLPPHSFSSFDLLKESAKI 2233
Score = 67.5 bits (141), Expect(3) = 2e-033
Identities = 25/35 (71%), Positives = 27/35 (77%)
Frame = +1 / +2
Query: 1 PLFVTDNDQTWNPDAIVFNSWQHYGTPSYWMQTXF 105
PLFV ND+ WNPDAIVFNS YGTPSYW+Q F
Sbjct: 1760 PLFVNTNDRRWNPDAIVFNSSHLYGTPSYWVQRFF 1864
Score = 38.6 bits (78), Expect(3) = 2e-033
Identities = 13/36 (36%), Positives = 25/36 (69%)
Frame = +1 / +2
Query: 181 ITWQDAENSFLRVKVVNFGSDTVNLTVSTSGLGASV 288
I+W++ ++R+K VNFG+++ N+ V +GL +V
Sbjct: 1937 ISWKNNGKDYIRIKAVNFGANSENMQVLVTGLDPNV 2044
>gb|AY243509.1| Arabidopsis thaliana alpha-L-arabinofuranosidase (ASD1) mRNA,
complete cds
Length = 2312
Score = 77.1 bits (162), Expect(3) = 2e-033
Identities = 35/59 (59%), Positives = 39/59 (66%)
Frame = +1 / +2
Query: 298 GSTATVLTSGNVMDENSFSNPNKVAPVKSQLSNAAEQMQVTLAPHSFTAFDLALAEPKL 474
GS TVLTS NVMDENSFS P KV P +S L A E M V L PHSF++FDL K+
Sbjct: 2018 GSKKTVLTSTNVMDENSFSQPEKVVPHESLLELAEEDMTVVLPPHSFSSFDLLKESAKI 2194
Score = 67.5 bits (141), Expect(3) = 2e-033
Identities = 25/35 (71%), Positives = 27/35 (77%)
Frame = +1 / +2
Query: 1 PLFVTDNDQTWNPDAIVFNSWQHYGTPSYWMQTXF 105
PLFV ND+ WNPDAIVFNS YGTPSYW+Q F
Sbjct: 1721 PLFVNTNDRRWNPDAIVFNSSHLYGTPSYWVQRFF 1825
Score = 38.6 bits (78), Expect(3) = 2e-033
Identities = 13/36 (36%), Positives = 25/36 (69%)
Frame = +1 / +2
Query: 181 ITWQDAENSFLRVKVVNFGSDTVNLTVSTSGLGASV 288
I+W++ ++R+K VNFG+++ N+ V +GL +V
Sbjct: 1898 ISWKNNGKDYIRIKAVNFGANSENMQVLVTGLDPNV 2005
>gb|AF372949.1|AF372949 Arabidopsis thaliana AT3g10740/T7M13_18 mRNA, complete cds
Length = 2254
Score = 77.1 bits (162), Expect(3) = 2e-033
Identities = 35/59 (59%), Positives = 39/59 (66%)
Frame = +1 / +3
Query: 298 GSTATVLTSGNVMDENSFSNPNKVAPVKSQLSNAAEQMQVTLAPHSFTAFDLALAEPKL 474
GS TVLTS NVMDENSFS P KV P +S L A E M V L PHSF++FDL K+
Sbjct: 1884 GSKKTVLTSTNVMDENSFSQPEKVVPHESLLELAEEDMTVVLPPHSFSSFDLLKESAKI 2060
Score = 67.5 bits (141), Expect(3) = 2e-033
Identities = 25/35 (71%), Positives = 27/35 (77%)
Frame = +1 / +3
Query: 1 PLFVTDNDQTWNPDAIVFNSWQHYGTPSYWMQTXF 105
PLFV ND+ WNPDAIVFNS YGTPSYW+Q F
Sbjct: 1587 PLFVNTNDRRWNPDAIVFNSSHLYGTPSYWVQRFF 1691
Score = 38.6 bits (78), Expect(3) = 2e-033
Identities = 13/36 (36%), Positives = 25/36 (69%)
Frame = +1 / +3
Query: 181 ITWQDAENSFLRVKVVNFGSDTVNLTVSTSGLGASV 288
I+W++ ++R+K VNFG+++ N+ V +GL +V
Sbjct: 1764 ISWKNNGKDYIRIKAVNFGANSENMQVLVTGLDPNV 1871
>gb|AY143944.1| Arabidopsis thaliana At3g10740/T7M13_18 mRNA, complete cds
Length = 2037
Score = 77.1 bits (162), Expect(3) = 2e-033
Identities = 35/59 (59%), Positives = 39/59 (66%)
Frame = +1 / +1
Query: 298 GSTATVLTSGNVMDENSFSNPNKVAPVKSQLSNAAEQMQVTLAPHSFTAFDLALAEPKL 474
GS TVLTS NVMDENSFS P KV P +S L A E M V L PHSF++FDL K+
Sbjct: 1810 GSKKTVLTSTNVMDENSFSQPEKVVPHESLLELAEEDMTVVLPPHSFSSFDLLKESAKI 1986
Score = 67.5 bits (141), Expect(3) = 2e-033
Identities = 25/35 (71%), Positives = 27/35 (77%)
Frame = +1 / +1
Query: 1 PLFVTDNDQTWNPDAIVFNSWQHYGTPSYWMQTXF 105
PLFV ND+ WNPDAIVFNS YGTPSYW+Q F
Sbjct: 1513 PLFVNTNDRRWNPDAIVFNSSHLYGTPSYWVQRFF 1617
Score = 38.6 bits (78), Expect(3) = 2e-033
Identities = 13/36 (36%), Positives = 25/36 (69%)
Frame = +1 / +1
Query: 181 ITWQDAENSFLRVKVVNFGSDTVNLTVSTSGLGASV 288
I+W++ ++R+K VNFG+++ N+ V +GL +V
Sbjct: 1690 ISWKNNGKDYIRIKAVNFGANSENMQVLVTGLDPNV 1797
>dbj|AK222175.1| Arabidopsis thaliana mRNA for putative alpha-L-arabinofuranosidase,
partial cds, clone: RAFL24-02-J05
Length = 1328
Score = 77.1 bits (162), Expect(3) = 2e-033
Identities = 35/59 (59%), Positives = 39/59 (66%)
Frame = +1 / +1
Query: 298 GSTATVLTSGNVMDENSFSNPNKVAPVKSQLSNAAEQMQVTLAPHSFTAFDLALAEPKL 474
GS TVLTS NVMDENSFS P KV P +S L A E M V L PHSF++FDL K+
Sbjct: 952 GSKKTVLTSTNVMDENSFSQPEKVVPHESLLELAEEDMTVVLPPHSFSSFDLLKESAKI 1128
Score = 67.5 bits (141), Expect(3) = 2e-033
Identities = 25/35 (71%), Positives = 27/35 (77%)
Frame = +1 / +1
Query: 1 PLFVTDNDQTWNPDAIVFNSWQHYGTPSYWMQTXF 105
PLFV ND+ WNPDAIVFNS YGTPSYW+Q F
Sbjct: 655 PLFVNTNDRRWNPDAIVFNSSHLYGTPSYWVQRFF 759
Score = 38.6 bits (78), Expect(3) = 2e-033
Identities = 13/36 (36%), Positives = 25/36 (69%)
Frame = +1 / +1
Query: 181 ITWQDAENSFLRVKVVNFGSDTVNLTVSTSGLGASV 288
I+W++ ++R+K VNFG+++ N+ V +GL +V
Sbjct: 832 ISWKNNGKDYIRIKAVNFGANSENMQVLVTGLDPNV 939
>dbj|AK220766.1| Arabidopsis thaliana mRNA for putative alpha-L-arabinofuranosidase,
partial cds, clone: RAFL22-21-H07
Length = 993
Score = 77.1 bits (162), Expect(3) = 2e-033
Identities = 35/59 (59%), Positives = 39/59 (66%)
Frame = +1 / +2
Query: 298 GSTATVLTSGNVMDENSFSNPNKVAPVKSQLSNAAEQMQVTLAPHSFTAFDLALAEPKL 474
GS TVLTS NVMDENSFS P KV P +S L A E M V L PHSF++FDL K+
Sbjct: 617 GSKKTVLTSTNVMDENSFSQPEKVVPHESLLELAEEDMTVVLPPHSFSSFDLLKESAKI 793
Score = 67.5 bits (141), Expect(3) = 2e-033
Identities = 25/35 (71%), Positives = 27/35 (77%)
Frame = +1 / +2
Query: 1 PLFVTDNDQTWNPDAIVFNSWQHYGTPSYWMQTXF 105
PLFV ND+ WNPDAIVFNS YGTPSYW+Q F
Sbjct: 320 PLFVNTNDRRWNPDAIVFNSSHLYGTPSYWVQRFF 424
Score = 38.6 bits (78), Expect(3) = 2e-033
Identities = 13/36 (36%), Positives = 25/36 (69%)
Frame = +1 / +2
Query: 181 ITWQDAENSFLRVKVVNFGSDTVNLTVSTSGLGASV 288
I+W++ ++R+K VNFG+++ N+ V +GL +V
Sbjct: 497 ISWKNNGKDYIRIKAVNFGANSENMQVLVTGLDPNV 604
>gb|BX836814.1|BX836814 BX836814 Arabidopsis thaliana Flowers and buds Col-0 Arabidopsis
thaliana cDNA clone GSLTFB91ZE06 3PRIM, mRNA sequence
Length = 1096
Score = 77.1 bits (162), Expect(3) = 7e-032
Identities = 35/59 (59%), Positives = 39/59 (66%)
Frame = +1 / -1
Query: 298 GSTATVLTSGNVMDENSFSNPNKVAPVKSQLSNAAEQMQVTLAPHSFTAFDLALAEPKL 474
GS TVLTS NVMDENSFS P KV P +S L A E M V L PHSF++FDL K+
Sbjct: 331 GSKKTVLTSTNVMDENSFSQPEKVVPHESLLELAEEDMTVVLPPHSFSSFDLLKESAKI 155
Score = 68.0 bits (142), Expect(3) = 7e-032
Identities = 25/35 (71%), Positives = 27/35 (77%)
Frame = +1 / -3
Query: 1 PLFVTDNDQTWNPDAIVFNSWQHYGTPSYWMQTXF 105
PLFV ND+ WNPDAIVFNS YGTPSYW+Q F
Sbjct: 629 PLFVNTNDRRWNPDAIVFNSSHLYGTPSYWVQRSF 525
Score = 32.7 bits (65), Expect(3) = 7e-032
Identities = 11/33 (33%), Positives = 22/33 (66%)
Frame = +1 / -3
Query: 181 ITWQDAENSFLRVKVVNFGSDTVNLTVSTSGLG 279
I+W++ +R+K VNFG+++ N+ V + +G
Sbjct: 452 ISWKNNGKDHIRIKAVNFGANSENMQVFGNWIG 354
>emb|BX824011.1|CNS0A4VB Arabidopsis thaliana Full-length cDNA Complete sequence from clone
GSLTPGH14ZD10 of Hormone Treated Callus of strain col-0
of Arabidopsis thaliana (thale cress)
Length = 2351
Score = 67.5 bits (141), Expect(4) = 3e-029
Identities = 25/35 (71%), Positives = 27/35 (77%)
Frame = +1 / +1
Query: 1 PLFVTDNDQTWNPDAIVFNSWQHYGTPSYWMQTXF 105
PLFV ND+ WNPDAIVFNS YGTPSYW+Q F
Sbjct: 1762 PLFVNTNDRRWNPDAIVFNSSHLYGTPSYWVQRFF 1866
Score = 55.1 bits (114), Expect(4) = 3e-029
Identities = 26/40 (65%), Positives = 27/40 (67%)
Frame = +1 / +1
Query: 298 GSTATVLTSGNVMDENSFSNPNKVAPVKSQLSNAAEQMQV 417
GS TVLTS NVMDENSFS P KV P +S L A E M V
Sbjct: 2059 GSKKTVLTSTNVMDENSFSQPEKVVPHESLLELAEEDMTV 2178
Score = 38.6 bits (78), Expect(4) = 3e-029
Identities = 13/36 (36%), Positives = 25/36 (69%)
Frame = +1 / +1
Query: 181 ITWQDAENSFLRVKVVNFGSDTVNLTVSTSGLGASV 288
I+W++ ++R+K VNFG+++ N+ V +GL +V
Sbjct: 1939 ISWKNNGKDYIRIKAVNFGANSENMQVLVTGLDPNV 2046
Score = 24.9 bits (48), Expect(4) = 3e-029
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +1 / +2
Query: 421 LAPHSFTAFDLALAEPKL 474
L PHSF++FDL K+
Sbjct: 2183 LPPHSFSSFDLLKESAKI 2236
>gb|AY063925.1| Arabidopsis thaliana putative arabinosidase (At5g26120) mRNA,
complete cds
Length = 2219
Score = 62.9 bits (131), Expect(3) = 3e-023
Identities = 24/35 (68%), Positives = 26/35 (74%)
Frame = +1 / +1
Query: 1 PLFVTDNDQTWNPDAIVFNSWQHYGTPSYWMQTXF 105
PLFV ND+ W PDAIVFNS YGTPSYW+Q F
Sbjct: 1585 PLFVNTNDRRWIPDAIVFNSSHLYGTPSYWVQHFF 1689
Score = 49.2 bits (101), Expect(3) = 3e-023
Identities = 22/51 (43%), Positives = 29/51 (56%)
Frame = +1 / +1
Query: 277 GASVNALGSTATVLTSGNVMDENSFSNPNKVAPVKSQLSNAAEQMQVTLAP 429
G GS VLTS +VMDENSFSNPN + P +S L ++ + + P
Sbjct: 1852 GLMAKFYGSKKKVLTSASVMDENSFSNPNMIVPQESLLEMTEQEDLMFVLP 2004
Score = 36.3 bits (73), Expect(3) = 3e-023
Identities = 14/34 (41%), Positives = 23/34 (67%)
Frame = +1 / +1
Query: 181 ITWQDAENSFLRVKVVNFGSDTVNLTVSTSGLGA 282
I++Q ++++K VNFG +VNL V+ +GL A
Sbjct: 1762 ISFQTNGKDYIQIKAVNFGEQSVNLKVAVTGLMA 1863
>ref|NM_122513.3| Arabidopsis thaliana hydrolase, acting on glycosyl bonds AT5G26120
mRNA, complete cds
Length = 2208
Score = 62.9 bits (131), Expect(3) = 3e-023
Identities = 24/35 (68%), Positives = 26/35 (74%)
Frame = +1 / +1
Query: 1 PLFVTDNDQTWNPDAIVFNSWQHYGTPSYWMQTXF 105
PLFV ND+ W PDAIVFNS YGTPSYW+Q F
Sbjct: 1585 PLFVNTNDRRWIPDAIVFNSSHLYGTPSYWVQHFF 1689
Score = 49.2 bits (101), Expect(3) = 3e-023
Identities = 22/51 (43%), Positives = 29/51 (56%)
Frame = +1 / +1
Query: 277 GASVNALGSTATVLTSGNVMDENSFSNPNKVAPVKSQLSNAAEQMQVTLAP 429
G GS VLTS +VMDENSFSNPN + P +S L ++ + + P
Sbjct: 1852 GLMAKFYGSKKKVLTSASVMDENSFSNPNMIVPQESLLEMTEQEDLMFVLP 2004
Score = 36.3 bits (73), Expect(3) = 3e-023
Identities = 14/34 (41%), Positives = 23/34 (67%)
Frame = +1 / +1
Query: 181 ITWQDAENSFLRVKVVNFGSDTVNLTVSTSGLGA 282
I++Q ++++K VNFG +VNL V+ +GL A
Sbjct: 1762 ISFQTNGKDYIQIKAVNFGEQSVNLKVAVTGLMA 1863
>gb|BT006062.1| Arabidopsis thaliana clone U18147 unknown protein (At5g26120) mRNA,
complete cds
Length = 2055
Score = 62.9 bits (131), Expect(3) = 3e-023
Identities = 24/35 (68%), Positives = 26/35 (74%)
Frame = +1 / +1
Query: 1 PLFVTDNDQTWNPDAIVFNSWQHYGTPSYWMQTXF 105
PLFV ND+ W PDAIVFNS YGTPSYW+Q F
Sbjct: 1510 PLFVNTNDRRWIPDAIVFNSSHLYGTPSYWVQHFF 1614
Score = 49.2 bits (101), Expect(3) = 3e-023
Identities = 22/51 (43%), Positives = 29/51 (56%)
Frame = +1 / +1
Query: 277 GASVNALGSTATVLTSGNVMDENSFSNPNKVAPVKSQLSNAAEQMQVTLAP 429
G GS VLTS +VMDENSFSNPN + P +S L ++ + + P
Sbjct: 1777 GLMAKFYGSKKKVLTSASVMDENSFSNPNMIVPQESLLEMTEQEDLMFVLP 1929
Score = 36.3 bits (73), Expect(3) = 3e-023
Identities = 14/34 (41%), Positives = 23/34 (67%)
Frame = +1 / +1
Query: 181 ITWQDAENSFLRVKVVNFGSDTVNLTVSTSGLGA 282
I++Q ++++K VNFG +VNL V+ +GL A
Sbjct: 1687 ISFQTNGKDYIQIKAVNFGEQSVNLKVAVTGLMA 1788
>gb|AY243510.1| Arabidopsis thaliana alpha-L-arabinofuranosidase (ASD2) mRNA,
complete cds
Length = 2043
Score = 62.9 bits (131), Expect(3) = 3e-023
Identities = 24/35 (68%), Positives = 26/35 (74%)
Frame = +1 / +3
Query: 1 PLFVTDNDQTWNPDAIVFNSWQHYGTPSYWMQTXF 105
PLFV ND+ W PDAIVFNS YGTPSYW+Q F
Sbjct: 1515 PLFVNTNDRRWIPDAIVFNSSHLYGTPSYWVQHFF 1619
Score = 49.2 bits (101), Expect(3) = 3e-023
Identities = 22/51 (43%), Positives = 29/51 (56%)
Frame = +1 / +3
Query: 277 GASVNALGSTATVLTSGNVMDENSFSNPNKVAPVKSQLSNAAEQMQVTLAP 429
G GS VLTS +VMDENSFSNPN + P +S L ++ + + P
Sbjct: 1782 GLMAKFYGSKKKVLTSASVMDENSFSNPNMIVPQESLLEMTEQEDLMFVLP 1934
Score = 36.3 bits (73), Expect(3) = 3e-023
Identities = 14/34 (41%), Positives = 23/34 (67%)
Frame = +1 / +3
Query: 181 ITWQDAENSFLRVKVVNFGSDTVNLTVSTSGLGA 282
I++Q ++++K VNFG +VNL V+ +GL A
Sbjct: 1692 ISFQTNGKDYIQIKAVNFGEQSVNLKVAVTGLMA 1793
Database: At_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:09 PM
Number of letters in database: 908,940,872
Number of sequences in database: 1,013,581
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 1,697,203,687
Number of Sequences: 1013581
Number of extensions: 23820992
Number of successful extensions: 197632
Number of sequences better than 1.0e-020: 12
length of database: 302,980,290
effective HSP length: 56
effective length of database: 246,219,754
effective search space used: 51459928586
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)