BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2440419.2.1
(616 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BT008984.1| Triticum aestivum clone wdk2c.pk007.b15:fis,... 478 e-133
gb|BJ319189.1|BJ319189 BJ319189 Y. Ogihara unpublished cDNA... 313 8e-084
gb|CA608984.1|CA608984 wr1.pk0100.d9 wr1 Triticum aestivum ... 182 2e-044
gb|BT009516.1| Triticum aestivum clone wr1.pk0100.d9:fis, f... 182 2e-044
gb|BJ261392.1|BJ261392 BJ261392 Y. Ogihara unpublished cDNA... 161 7e-038
gb|BJ231160.1|BJ231160 BJ231160 Y. Ogihara unpublished cDNA... 157 1e-036
gb|BQ237253.1|BQ237253 TaE05019F09F TaE05 Triticum aestivum... 133 2e-029
gb|BJ228528.1|BJ228528 BJ228528 Y. Ogihara unpublished cDNA... 127 1e-027
gb|BQ166623.1|BQ166623 WHE0906_E01_I02ZT Wheat 5-15 DAP spi... 119 2e-025
gb|BE606819.1|BE606819 WHE0906_E01_I02ZS Wheat 5-15 DAP spi... 92 5e-017
gb|BQ240282.1|BQ240282 TaE05019F09R TaE05 Triticum aestivum... 92 5e-017
gb|CD936892.1|CD936892 OV.105G14F010220 OV Triticum aestivu... 84 1e-014
gb|BQ578381.1|BQ578381 WHE0302_C02_E04ZS Wheat unstressed s... 80 2e-013
gb|BJ279341.1|BJ279341 BJ279341 Y. Ogihara unpublished cDNA... 62 5e-008
gb|BQ743914.1|BQ743914 WHE4109_F08_K15ZS Wheat salt-stresse... 62 5e-008
gb|CD491640.1|CD491640 WHE2851_E09_I17ZT CS wheat unstresse... 46 0.003
gb|CV776321.1|CV776321 FGAS070725 Triticum aestivum FGAS: L... 44 0.011
gb|CD904146.1|CD904146 G356.112J16F010920 G356 Triticum aes... 40 0.18
gb|CD904147.1|CD904147 G356.112J16R011029 G356 Triticum aes... 40 0.18
>gb|BT008984.1| Triticum aestivum clone wdk2c.pk007.b15:fis, full insert mRNA
sequence
Length = 2056
Score = 478 bits (241), Expect = e-133
Identities = 393/444 (88%)
Strand = Plus / Minus
Query: 120 catgagcgcggtggccgnggcgccacaggatacagcctcgttgggcaccttggcgacgag 179
||||||||||||||||| |||||| ||||| || ||||| |||||||||||| ||||| |
Sbjct: 1798 catgagcgcggtggccgtggcgccgcaggagacggcctccttgggcaccttgccgacgcg 1739
Query: 180 gtcgacagcctcagcgaagatgacgcccatgcccatgtggaggtgcggctcgatatggca 239
||||| ||||| ||||||||||| ||||||||||||||||||||||||||||| |||||
Sbjct: 1738 gtcgatggcctcggcgaagatgacccccatgcccatgtggaggtgcggctcgatgtggca 1679
Query: 240 gtggaaggcccacacgcccgggttgtccgccacgaaccggagcatcgtccacccgtacgg 299
|||||| |||||||| || |||||||||||||||||||| ||| |||||||||||||||
Sbjct: 1678 gtggaacgcccacaccccggggttgtccgccacgaaccgcagcgccgtccacccgtacgg 1619
Query: 300 aaagatgaccgccgtgttccgcagcggcgggtcccggaggttgagccgcgcttcgtcggc 359
||||| ||||||||||||||||||||||||| | ||||| |||| ||| |||||||
Sbjct: 1618 gaagatcaccgccgtgttccgcagcggcgggttcaccaggttcagcctcgccgcgtcggc 1559
Query: 360 ggcgtccccgcggtacgcgccgtcgccgtagcccagcacccagaagtcgtggccgtggag 419
|||||| || | ||||||||| |||||||| |||||||||||||||||||||||||| ||
Sbjct: 1558 ggcgtcgcccctgtacgcgccctcgccgtaccccagcacccagaagtcgtggccgtgcag 1499
Query: 420 gtgccacgggtgcacctcgctgacgttgcgcgacagcgcgttggcgttctggagcagcac 479
|||||||||||||||||||||||||||| || |||||||||||||||||||||| |||
Sbjct: 1498 gtgccacgggtgcacctcgctgacgttgtgctggagcgcgttggcgttctggagcaccac 1439
Query: 480 gtcgacggtggtgttgtgcgccaggacgtacacgttgtcccccgccgtcgtgttggggtt 539
||| || ||||||||||| || ||||| ||||||||||||| |||||||||||||||
Sbjct: 1438 gtccaccgtggtgttgtggcggagcacgtagacgttgtcccccgtcgtcgtgttggggtt 1379
Query: 540 cggcggcggcagcgtcacgtcgta 563
|||||||| |||||||||||||
Sbjct: 1378 gtgcggcggccgcgtcacgtcgta 1355
>gb|BJ319189.1|BJ319189 BJ319189 Y. Ogihara unpublished cDNA library, Wh_yf Triticum
aestivum cDNA clone whyf5g20 3', mRNA sequence
Length = 289
Score = 313 bits (158), Expect = 8e-084
Identities = 235/261 (90%)
Strand = Plus / Plus
Query: 187 gcctcagcgaagatgacgcccatgcccatgtggaggtgcggctcgatatggcagtggaag 246
||||| ||||||||||| ||||||||||||||||||||||||||||| |||||||||||
Sbjct: 13 gcctcggcgaagatgacccccatgcccatgtggaggtgcggctcgatgtggcagtggaac 72
Query: 247 gcccacacgcccgggttgtccgccacgaaccggagcatcgtccacccgtacggaaagatg 306
|||||||| || |||||||||||||||||||| ||| ||||||||||||||| |||||
Sbjct: 73 gcccacaccccggggttgtccgccacgaaccgcagcgccgtccacccgtacgggaagatc 132
Query: 307 accgccgtgttccgcagcggcgggtcccggaggttgagccgcgcttcgtcggcggcgtcc 366
||||||||||||||||||||||||| | ||||| |||| ||| |||||||||||||
Sbjct: 133 accgccgtgttccgcagcggcgggttcaccaggttcagcctcgccgcgtcggcggcgtcg 192
Query: 367 ccgcggtacgcgccgtcgccgtagcccagcacccagaagtcgtggccgtggaggtgccac 426
|| | ||||||||| |||||||| |||||||||||||||||||||||||| |||||||||
Sbjct: 193 cccctgtacgcgccctcgccgtaccccagcacccagaagtcgtggccgtgcaggtgccac 252
Query: 427 gggtgcacctcgctgacgttg 447
|||||||||| ||||||||||
Sbjct: 253 gggtgcacctngctgacgttg 273
>gb|CA608984.1|CA608984 wr1.pk0100.d9 wr1 Triticum aestivum cDNA clone wr1.pk0100.d9 5'
end, mRNA sequence
Length = 416
Score = 182 bits (92), Expect = 2e-044
Identities = 142/159 (89%)
Strand = Plus / Minus
Query: 120 catgagcgcggtggccgnggcgccacaggatacagcctcgttgggcaccttggcgacgag 179
||||||||||||||||| |||||| ||||| || ||||| ||||||||||| ||||| |
Sbjct: 169 catgagcgcggtggccgtggcgccgcaggagacggcctccttgggcaccttcccgacgcg 110
Query: 180 gtcgacagcctcagcgaagatgacgcccatgcccatgtggaggtgcggctcgatatggca 239
||||| ||||| ||||||||||| ||||| ||||||||||||||||||||||| |||||
Sbjct: 109 gtcgatggcctcggcgaagatgacccccatccccatgtggaggtgcggctcgatgtggca 50
Query: 240 gtggaaggcccacacgcccgggttgtccgccacgaaccg 278
|||||| |||||||| || ||||||||||||||||||||
Sbjct: 49 gtggaacgcccacaccccggggttgtccgccacgaaccg 11
>gb|BT009516.1| Triticum aestivum clone wr1.pk0100.d9:fis, full insert mRNA
sequence
Length = 491
Score = 182 bits (92), Expect = 2e-044
Identities = 142/159 (89%)
Strand = Plus / Minus
Query: 120 catgagcgcggtggccgnggcgccacaggatacagcctcgttgggcaccttggcgacgag 179
||||||||||||||||| |||||| ||||| || ||||| ||||||||||| ||||| |
Sbjct: 176 catgagcgcggtggccgtggcgccgcaggagacggcctccttgggcaccttcccgacgcg 117
Query: 180 gtcgacagcctcagcgaagatgacgcccatgcccatgtggaggtgcggctcgatatggca 239
||||| ||||| ||||||||||| ||||| ||||||||||||||||||||||| |||||
Sbjct: 116 gtcgatggcctcggcgaagatgacccccatccccatgtggaggtgcggctcgatgtggca 57
Query: 240 gtggaaggcccacacgcccgggttgtccgccacgaaccg 278
|||||| |||||||| || ||||||||||||||||||||
Sbjct: 56 gtggaacgcccacaccccggggttgtccgccacgaaccg 18
>gb|BJ261392.1|BJ261392 BJ261392 Y. Ogihara unpublished cDNA library, Wh_h Triticum
aestivum cDNA clone whh2l10 3', mRNA sequence
Length = 328
Score = 161 bits (81), Expect = 7e-038
Identities = 131/149 (87%)
Strand = Plus / Plus
Query: 120 catgagcgcggtggccgnggcgccacaggatacagcctcgttgggcaccttggcgacgag 179
||||||||||||||||| |||||| || || || ||||| |||||||||||| ||||| |
Sbjct: 180 catgagcgcggtggccgtggcgccgcangagacggcctccttgggcaccttgccgacgcg 239
Query: 180 gtcgacagcctcagcgaagatgacgcccatgcccatgtggaggtgcggctcgatatggca 239
||||| ||||| ||||||||||| ||||||||||||||||||||||||||||| ||| |
Sbjct: 240 gtcgatggcctcggcgaagatgacccccatgcccatgtggaggtgcggctcgatgtggna 299
Query: 240 gtggaaggcccacacgcccgggttgtccg 268
||||| |||||||| || ||||||||||
Sbjct: 300 ntggaacgcccacaccccggggttgtccg 328
>gb|BJ231160.1|BJ231160 BJ231160 Y. Ogihara unpublished cDNA library, Wh_dL Triticum
aestivum cDNA clone whdl25l06 3', mRNA sequence
Length = 359
Score = 157 bits (79), Expect = 1e-036
Identities = 131/149 (87%)
Strand = Plus / Plus
Query: 120 catgagcgcggtggccgnggcgccacaggatacagcctcgttgggcaccttggcgacgag 179
||||||||||||||||| |||||| ||||| || ||||| ||||||||||| ||||| |
Sbjct: 211 catgagcgcggtggccgtggcgccgcaggagacggcctccttgggcaccttcccgacgcg 270
Query: 180 gtcgacagcctcagcgaagatgacgcccatgcccatgtggaggtgcggctcgatatggca 239
||||| ||||| ||||||||||| ||||| ||||||||||||||||||||||| |||||
Sbjct: 271 gtcgatggcctcngcgaagatgacccccatccccatgtggaggtgcggctcgatgtggca 330
Query: 240 gtggaaggcccacacgcccgggttgtccg 268
|||||| |||||||| || |||||||||
Sbjct: 331 gtggaacgcccacaccccgtggttgtccg 359
>gb|BQ237253.1|BQ237253 TaE05019F09F TaE05 Triticum aestivum cDNA clone TaE05019F09F, mRNA
sequence
Length = 366
Score = 133 bits (67), Expect = 2e-029
Identities = 115/131 (87%)
Strand = Plus / Plus
Query: 203 cgcccatgcccatgtggaggtgcggctcgatatggcagtggaaggcccacacgcccgggt 262
||||||||||||||||||||||||||||||| ||||||||||| |||||| ||||| ||
Sbjct: 83 cgcccatgcccatgtggaggtgcggctcgatgtggcagtggaacgcccacgcgcccacgt 142
Query: 263 tgtccgccacgaaccggagcatcgtccacccgtacggaaagatgaccgccgtgttccgca 322
|||| || |||||||||| | ||||||||||| ||| || | ||| ||||||||||||
Sbjct: 143 tgtcggcgacgaaccggatcgccgtccacccgtgcgggaacaacaccaccgtgttccgca 202
Query: 323 gcggcgggtcc 333
|||||||||||
Sbjct: 203 gcggcgggtcc 213
Score = 91.7 bits (46), Expect = 5e-017
Identities = 61/66 (92%)
Strand = Plus / Plus
Query: 382 tcgccgtagcccagcacccagaagtcgtggccgtggaggtgccacgggtgcacctcgctg 441
|||||||| |||||||||||||||||||||||||| ||||||||||||||| |||||||
Sbjct: 259 tcgccgtaccccagcacccagaagtcgtggccgtgcaggtgccacgggtgcgtctcgctg 318
Query: 442 acgttg 447
|||||
Sbjct: 319 tcgttg 324
>gb|BJ228528.1|BJ228528 BJ228528 Y. Ogihara unpublished cDNA library, Wh_dL Triticum
aestivum cDNA clone whdl6m17 3', mRNA sequence
Length = 651
Score = 127 bits (64), Expect = 1e-027
Identities = 121/140 (86%)
Strand = Plus / Plus
Query: 194 cgaagatgacgcccatgcccatgtggaggtgcggctcgatatggcagtggaaggcccaca 253
|||||| | ||||||||||||||||||||||||||||||| ||||||||||| ||||||
Sbjct: 281 cgaagacggcgcccatgcccatgtggaggtgcggctcgatgtggcagtggaacgcccacg 340
Query: 254 cgcccgggttgtccgccacgaaccggagcatcgtccacccgtacggaaagatgaccgccg 313
||||| |||||| || |||||||||| | |||||| |||| ||| || | ||| |||
Sbjct: 341 cgcccacgttgtcggcgacgaaccggatcgccgtccagccgtgcgggaacaccaccaccg 400
Query: 314 tgttccgcagcggcgggtcc 333
||||||||||||||||||||
Sbjct: 401 tgttccgcagcggcgggtcc 420
Score = 91.7 bits (46), Expect = 5e-017
Identities = 61/66 (92%)
Strand = Plus / Plus
Query: 382 tcgccgtagcccagcacccagaagtcgtggccgtggaggtgccacgggtgcacctcgctg 441
|||||||| |||||||||||||||||||||||||| ||||||||||||||| |||||||
Sbjct: 460 tcgccgtaccccagcacccagaagtcgtggccgtgcaggtgccacgggtgcgtctcgctg 519
Query: 442 acgttg 447
|||||
Sbjct: 520 tcgttg 525
>gb|BQ166623.1|BQ166623 WHE0906_E01_I02ZT Wheat 5-15 DAP spike cDNA library Triticum
aestivum cDNA clone WHE0906_E01_I02, mRNA sequence
Length = 628
Score = 119 bits (60), Expect = 2e-025
Identities = 115/132 (87%), Gaps = 1/132 (0%)
Strand = Plus / Plus
Query: 203 cgcccatgccca-tgtggaggtgcggctcgatatggcagtggaaggcccacacgcccggg 261
|||||||||||| ||||||||||||||||||| ||||||||||| |||||| ||||| |
Sbjct: 312 cgcccatgcccagtgtggaggtgcggctcgatgtggcagtggaacgcccacgcgcccacg 371
Query: 262 ttgtccgccacgaaccggagcatcgtccacccgtacggaaagatgaccgccgtgttccgc 321
||||| || |||||||||| | ||||||||||| ||| || | ||| |||||||||||
Sbjct: 372 ttgtcggcgacgaaccggatcgccgtccacccgtgcgggaacaacaccaccgtgttccgc 431
Query: 322 agcggcgggtcc 333
||||||||||||
Sbjct: 432 agcggcgggtcc 443
Score = 54.0 bits (27), Expect = 1e-005
Identities = 57/66 (86%), Gaps = 1/66 (1%)
Strand = Plus / Plus
Query: 382 tcgccgtagcccagcacccagaagtcgtggccgtggaggtgccacgggtgcacctcgctg 441
|||||||| ||||||||| |||||||||||||| ||||| ||||||||| |||||||
Sbjct: 489 tcgccgtaccccagcaccagnaagtcgtggccgtgcaggtg-cacgggtgcgtctcgctg 547
Query: 442 acgttg 447
|||||
Sbjct: 548 tcgttg 553
>gb|BE606819.1|BE606819 WHE0906_E01_I02ZS Wheat 5-15 DAP spike cDNA library Triticum
aestivum cDNA clone WHE0906_E01_I02, mRNA sequence
Length = 315
Score = 91.7 bits (46), Expect = 5e-017
Identities = 61/66 (92%)
Strand = Plus / Minus
Query: 382 tcgccgtagcccagcacccagaagtcgtggccgtggaggtgccacgggtgcacctcgctg 441
|||||||| |||||||||||||||||||||||||| ||||||||||||||| |||||||
Sbjct: 306 tcgccgtaccccagcacccagaagtcgtggccgtgcaggtgccacgggtgcgtctcgctg 247
Query: 442 acgttg 447
|||||
Sbjct: 246 tcgttg 241
>gb|BQ240282.1|BQ240282 TaE05019F09R TaE05 Triticum aestivum cDNA clone TaE05019F09R, mRNA
sequence
Length = 500
Score = 91.7 bits (46), Expect = 5e-017
Identities = 61/66 (92%)
Strand = Plus / Minus
Query: 382 tcgccgtagcccagcacccagaagtcgtggccgtggaggtgccacgggtgcacctcgctg 441
|||||||| |||||||||||||||||||||||||| ||||||||||||||| |||||||
Sbjct: 479 tcgccgtaccccagcacccagaagtcgtggccgtgcaggtgccacgggtgcgtctcgctg 420
Query: 442 acgttg 447
|||||
Sbjct: 419 tcgttg 414
>gb|CD936892.1|CD936892 OV.105G14F010220 OV Triticum aestivum cDNA clone OV105G14, mRNA
sequence
Length = 662
Score = 83.8 bits (42), Expect = 1e-014
Identities = 60/66 (90%)
Strand = Plus / Minus
Query: 201 gacgcccatgcccatgtggaggtgcggctcgatatggcagtggaaggcccacacgcccgg 260
|||||||||||||||| || ||||| |||||| ||||||||||||||||||||||| ||
Sbjct: 332 gacgcccatgcccatgaagaagtgcgcctcgatgtggcagtggaaggcccacacgccggg 273
Query: 261 gttgtc 266
||||||
Sbjct: 272 gttgtc 267
Score = 79.8 bits (40), Expect = 2e-013
Identities = 73/84 (86%)
Strand = Plus / Minus
Query: 399 ccagaagtcgtggccgtggaggtgccacgggtgcacctcgctgacgttgcgcgacagcgc 458
||||||||||||||||||||||||||||||||| ||||||| |||| ||| ||||
Sbjct: 136 ccagaagtcgtggccgtggaggtgccacgggtgggtctcgctgttgttgggcgccagcat 77
Query: 459 gttggcgttctggagcagcacgtc 482
|||||||||||| |||| ||||||
Sbjct: 76 gttggcgttctgcagcaccacgtc 53
>gb|BQ578381.1|BQ578381 WHE0302_C02_E04ZS Wheat unstressed seedling shoot cDNA library
Triticum aestivum cDNA clone WHE0302_C02_E04, mRNA
sequence
Length = 574
Score = 79.8 bits (40), Expect = 2e-013
Identities = 73/84 (86%)
Strand = Plus / Minus
Query: 399 ccagaagtcgtggccgtggaggtgccacgggtgcacctcgctgacgttgcgcgacagcgc 458
||||||||||||||||||||||||||||||||| ||||||| |||| ||| ||||
Sbjct: 564 ccagaagtcgtggccgtggaggtgccacgggtgggtctcgctgttgttgggcgccagcat 505
Query: 459 gttggcgttctggagcagcacgtc 482
|||||||||||| |||| ||||||
Sbjct: 504 gttggcgttctgcagcaccacgtc 481
>gb|BJ279341.1|BJ279341 BJ279341 Y. Ogihara unpublished cDNA library, Wh_r Triticum
aestivum cDNA clone whr2i02 5', mRNA sequence
Length = 599
Score = 61.9 bits (31), Expect = 5e-008
Identities = 46/51 (90%)
Strand = Plus / Minus
Query: 382 tcgccgtagcccagcacccagaagtcgtggccgtggaggtgccacgggtgc 432
||||||||||| ||||||||||||||||| || ||||| ||||| ||||||
Sbjct: 395 tcgccgtagccgagcacccagaagtcgtgcccatggagatgccatgggtgc 345
>gb|BQ743914.1|BQ743914 WHE4109_F08_K15ZS Wheat salt-stressed root cDNA library Triticum
aestivum cDNA clone WHE4109_F08_K15, mRNA sequence
Length = 692
Score = 61.9 bits (31), Expect = 5e-008
Identities = 46/51 (90%)
Strand = Plus / Minus
Query: 382 tcgccgtagcccagcacccagaagtcgtggccgtggaggtgccacgggtgc 432
||||||| ||| ||||||||||||||||| || ||||| ||||||||||||
Sbjct: 576 tcgccgtggccgagcacccagaagtcgtgaccatggagatgccacgggtgc 526
>gb|CD491640.1|CD491640 WHE2851_E09_I17ZT CS wheat unstressed root tip cDNA library
Triticum aestivum cDNA clone WHE2851_E09_I17, mRNA
sequence
Length = 629
Score = 46.1 bits (23), Expect = 0.003
Identities = 26/27 (96%)
Strand = Plus / Plus
Query: 249 ccacacgcccgggttgtccgccacgaa 275
|||||| ||||||||||||||||||||
Sbjct: 322 ccacacccccgggttgtccgccacgaa 348
>gb|CV776321.1|CV776321 FGAS070725 Triticum aestivum FGAS: Library 2 Gate 3 Triticum
aestivum cDNA, mRNA sequence
Length = 832
Score = 44.1 bits (22), Expect = 0.011
Identities = 22/22 (100%)
Strand = Plus / Plus
Query: 43 gcatgcgcgcgaggcaaggcgc 64
||||||||||||||||||||||
Sbjct: 312 gcatgcgcgcgaggcaaggcgc 333
>gb|CD904146.1|CD904146 G356.112J16F010920 G356 Triticum aestivum cDNA clone G356112J16,
mRNA sequence
Length = 595
Score = 40.1 bits (20), Expect = 0.18
Identities = 23/24 (95%)
Strand = Plus / Minus
Query: 360 ggcgtccccgcggtacgcgccgtc 383
||||||||||||| ||||||||||
Sbjct: 496 ggcgtccccgcggcacgcgccgtc 473
>gb|CD904147.1|CD904147 G356.112J16R011029 G356 Triticum aestivum cDNA clone G356112J16,
mRNA sequence
Length = 659
Score = 40.1 bits (20), Expect = 0.18
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 360 ggcgtccccgcggtacgcgccgtc 383
||||||||||||| ||||||||||
Sbjct: 409 ggcgtccccgcggcacgcgccgtc 432
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 193,217
Number of Sequences: 636343
Number of extensions: 193217
Number of successful extensions: 55642
Number of sequences better than 0.5: 19
Number of HSP's better than 0.5 without gapping: 19
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 55607
Number of HSP's gapped (non-prelim): 33
length of query: 616
length of database: 367,240,239
effective HSP length: 19
effective length of query: 597
effective length of database: 355,149,722
effective search space: 212024384034
effective search space used: 212024384034
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)