BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBTB.064G21F020918.3.1
(705 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CD209204.1|CD209204 HS1_46_F10.g1_A012 Heat-shocked seed... 581 e-164
gb|BE598686.1|BE598686 PI1_81_F04.b1_A002 Pathogen induced ... 408 e-112
gb|CD432412.1|CD432412 ETH1_29_A04.g1_A002 Ethylene-treated... 406 e-111
gb|CW428236.1|CW428236 fsbb001f140l08k0 Sorghum methylation... 295 3e-078
gb|CW415759.1|CW415759 fsbb001f116a03f0 Sorghum methylation... 274 1e-071
gb|BG464223.1|BG464223 EM1_71_B05.b1_A002 Embryo 1 (EM1) So... 248 6e-064
gb|BG649135.1|BG649135 EM1_56_A05.b1_A002 Embryo 1 (EM1) So... 248 6e-064
gb|CW108361.1|CW108361 104_480_11097488_116_34496_032 Sorgh... 115 6e-024
gb|CL164400.1|CL164400 104_358_10807882_114_31814_250 Sorgh... 42 0.068
gb|CW397078.1|CW397078 fsbb001f085n01f0 Sorghum methylation... 42 0.068
>gb|CD209204.1|CD209204 HS1_46_F10.g1_A012 Heat-shocked seedlings Sorghum bicolor cDNA
clone HS1_46_F10_A012 5', mRNA sequence
Length = 570
Score = 581 bits (293), Expect = e-164
Identities = 469/519 (90%), Gaps = 14/519 (2%)
Strand = Plus / Plus
Query: 4 agtagggagtgctcacaccatttcttgtccattgacatcagcgctccacggcctgcgccc 63
||||||||||||| |||||||||| | |||||| |||||||| ||| ||| |||||||||
Sbjct: 64 agtagggagtgcttacaccatttcctctccattcacatcagctctcgacg-cctgcgccc 122
Query: 64 ccggaattcccaaatcctgcggcgcctccgccccaaccccgctcgtcgactcctctcttc 123
|| ||||||||||| ||||| ||||||||||||||||| |||||||||||||||||| |
Sbjct: 123 cc--aattcccaaattctgcgccgcctccgccccaaccctgctcgtcgactcctctctgc 180
Query: 124 cgaacacgccggaggaatccttctccgcgactcgtcttcctcctctctgccgtgctggcg 183
||||||||||||||||||||||||||| |||||||||||||||||||||||| |||||||
Sbjct: 181 cgaacacgccggaggaatccttctccgagactcgtcttcctcctctctgccgggctggcg 240
Query: 184 tggagtggagatccaccaccacctggattggtctcttcttctgctggttagacgctgaat 243
||||||||||||||| |||||| | |||||||||||||||||||||||||| | ||||||
Sbjct: 241 tggagtggagatccatcaccacgtagattggtctcttcttctgctggttaggcactgaat 300
Query: 244 ctgtccttcaatgccttcacaggctctattcctgaagaagttgcagagttagaagcaatt 303
||| ||||||||||||||||||||||||| |||||||||||||||| | |||||| |||
Sbjct: 301 ctgcccttcaatgccttcacaggctctatccctgaagaagttgcagtttaagaagctatt 360
Query: 304 gccgtgttttttaattgaagggaacaaattttcgggtcatatccagattggatacggaac 363
||| |||||| ||||| ||||||| |||| ||||| |||||||||||| |||||||
Sbjct: 361 gccacgttttt--gttgaa-ggaacaattttttgggtc--atccagattggacacggaac 415
Query: 364 atgtgaaag--ctgtccttttgggaccttaagagaatacaaagcaaaaggaacataaggg 421
||||||||| ||||||||||||||| ||||||||| || |||||||||||||||||
Sbjct: 416 atgtgaaagctctgtccttttgggac---aagagaata-aaggcaaaaggaacataagga 471
Query: 422 tagaggacggaaataaacaatgggaaagggtgatgtggctactaggtccaaatctcaaaa 481
||||||| ||||||||||||||||||||| |||||||||||||||||||||||||||||
Sbjct: 472 tagaggagagaaataaacaatgggaaagggagatgtggctactaggtccaaatctcaaaa 531
Query: 482 atcgtcagcaatacagaatgagccgagtacacctgctaa 520
||||||||||||||||||||||| |||||||||| ||||
Sbjct: 532 atcgtcagcaatacagaatgagcagagtacacctactaa 570
>gb|BE598686.1|BE598686 PI1_81_F04.b1_A002 Pathogen induced 1 (PI1) Sorghum bicolor cDNA,
mRNA sequence
Length = 558
Score = 408 bits (206), Expect = e-112
Identities = 299/333 (89%), Gaps = 4/333 (1%)
Strand = Plus / Plus
Query: 373 ctgtccttttgggaccttaagagaatacaaagcaaaaggaacataagggtagaggacgga 432
||||||||||||||| ||||||||| || ||||||||||||||||| ||||||| ||
Sbjct: 217 ctgtccttttgggac---aagagaata-aaggcaaaaggaacataaggatagaggagaga 272
Query: 433 aataaacaatgggaaagggtgatgtggctactaggtccaaatctcaaaaatcgtcagcaa 492
||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct: 273 aataaacaatgggaaagggagatgtggctactaggtccaaatctcaaaaatcgtcagcaa 332
Query: 493 tacagaatgagccgagtacacctgctaannnnnnnacagcatatcctgattggtctcagt 552
|||||||||||| |||||||||| |||| |||||||||||||||||||||||||
Sbjct: 333 tacagaatgagcagagtacacctactaacccccccacagcatatcctgattggtctcagt 392
Query: 553 tccaggcatactataatgctgctgggacagctccagtaaccccaccggcnnnnnnncatt 612
|||||||||||||||||||| ||||||||||||| || ||||||||||| ||||
Sbjct: 393 tccaggcatactataatgctcctgggacagctcccgtgaccccaccggctttttttcatt 452
Query: 613 catctgtagctccaacccatcaggggcacccgtatatgtggggtccacagatgatgcctc 672
||||||||||||||| || |||||| ||||| |||||||||||||||||||||||||||
Sbjct: 453 catctgtagctccaagccctcagggacacccatatatgtggggtccacagatgatgcctn 512
Query: 673 cttatgggacaccaccaccatatgcaacaatgt 705
|||||||||||||||||||||||||| ||||||
Sbjct: 513 cttatgggacaccaccaccatatgcagcaatgt 545
Score = 170 bits (86), Expect = 1e-040
Identities = 162/184 (88%), Gaps = 5/184 (2%)
Strand = Plus / Plus
Query: 183 gtggagtggagatccaccaccacctggattggtctcttcttctgctggttagacgctgaa 242
|||||||||||||||| |||||| | |||||||||||||||||||||||||| | |||||
Sbjct: 9 gtggagtggagatccatcaccacgtagattggtctcttcttctgctggttaggcactgaa 68
Query: 243 tctgtccttcaatgccttcacaggctctattcctgaagaagttgcagagttagaagcaat 302
|||| ||||||||||||||||||||||||| |||||||||||||||| | |||||| ||
Sbjct: 69 tctgcccttcaatgccttcacaggctctatccctgaagaagttgcagtttaagaagctat 128
Query: 303 tgccgtgttttttaattgaagggaacaaattttcgggtcatatccagattggatacggaa 362
|||| |||||| ||||| ||||||| |||| ||||| |||||||||||| ||||||
Sbjct: 129 tgccacgttttt--gttgaa-ggaacaattttttgggtc--atccagattggacacggaa 183
Query: 363 catg 366
||||
Sbjct: 184 catg 187
>gb|CD432412.1|CD432412 ETH1_29_A04.g1_A002 Ethylene-treated seedlings Sorghum bicolor cDNA
clone ETH1_29_A04_A002 5', mRNA sequence
Length = 700
Score = 406 bits (205), Expect = e-111
Identities = 299/333 (89%), Gaps = 4/333 (1%)
Strand = Plus / Plus
Query: 373 ctgtccttttgggaccttaagagaatacaaagcaaaaggaacataagggtagaggacgga 432
||||||||||||||| ||||||||| || ||||||||||||||||| ||||||| ||
Sbjct: 367 ctgtccttttgggac---aagagaata-aaggcaaaaggaacataaggatagaggagaga 422
Query: 433 aataaacaatgggaaagggtgatgtggctactaggtccaaatctcaaaaatcgtcagcaa 492
||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct: 423 aataaacaatgggaaagggagatgtggctactaggtccaaatctcaaaaatcgtcagcaa 482
Query: 493 tacagaatgagccgagtacacctgctaannnnnnnacagcatatcctgattggtctcagt 552
|||||||||||| |||||||||| |||| |||||||||||||||||||||||||
Sbjct: 483 tacagaatgagcagagtacacctactaacccccccacagcatatcctgattggtctcagt 542
Query: 553 tccaggcatactataatgctgctgggacagctccagtaaccccaccggcnnnnnnncatt 612
|||||||||||||||||||| ||||||||||||| || ||||||||||| ||||
Sbjct: 543 tccaggcatactataatgctcctgggacagctcccgtgaccccaccggctttttttcatt 602
Query: 613 catctgtagctccaacccatcaggggcacccgtatatgtggggtccacagatgatgcctc 672
||||||||||||||| || |||||| ||||| ||||||||||||| ||||||||||||||
Sbjct: 603 catctgtagctccaagccctcagggacacccatatatgtggggtctacagatgatgcctc 662
Query: 673 cttatgggacaccaccaccatatgcaacaatgt 705
|||||||||||||||||||||||||| ||||||
Sbjct: 663 cttatgggacaccaccaccatatgcagcaatgt 695
Score = 363 bits (183), Expect = 1e-098
Identities = 302/336 (89%), Gaps = 8/336 (2%)
Strand = Plus / Plus
Query: 31 tccattgacatcagcgctccacggcctgcgcccccggaattcccaaatcctgcggcgcct 90
|||||| |||||||| ||| ||| ||||||||||| ||||||||||| ||||| |||||
Sbjct: 10 tccattcacatcagctctcgacg-cctgcgccccc--aattcccaaattctgcgccgcct 66
Query: 91 ccgccccaaccccgctcgtcgactcctctcttccgaacacgccggaggaatccttctccg 150
|||||||||||| |||||||||||||||||| ||||||||||||||||||||||||||||
Sbjct: 67 ccgccccaaccctgctcgtcgactcctctctgccgaacacgccggaggaatccttctccg 126
Query: 151 cgactcgtcttcctcctctctgccgtgctggcgtggagtggagatccaccaccacctgga 210
|||||||||||||||||||||||| |||||||||||||||||||||| |||||| | ||
Sbjct: 127 agactcgtcttcctcctctctgccgggctggcgtggagtggagatccatcaccacgtaga 186
Query: 211 ttggtctcttcttctgctggttagacgctgaatctgtccttcaatgccttcacaggctct 270
|||||||||||||||||||||||| | ||||||||| |||||||||||||||||||||||
Sbjct: 187 ttggtctcttcttctgctggttaggcactgaatctgcccttcaatgccttcacaggctct 246
Query: 271 attcctgaagaagttgcagagttagaagcaattgccgtgttttttaattgaagggaacaa 330
|| |||||||||||||||| | |||||| |||||| |||||| ||||| |||||||
Sbjct: 247 atccctgaagaagttgcagtttaagaagctattgccacgttttt--gttgaa-ggaacaa 303
Query: 331 attttcgggtcatatccagattggatacggaacatg 366
|||| ||||| |||||||||||| ||||||||||
Sbjct: 304 ttttttgggtc--atccagattggacacggaacatg 337
>gb|CW428236.1|CW428236 fsbb001f140l08k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f140l08, DNA
sequence
Length = 560
Score = 295 bits (149), Expect = 3e-078
Identities = 210/228 (92%), Gaps = 3/228 (1%)
Strand = Plus / Plus
Query: 4 agtagggagtgctcacaccatttcttgtccattgacatcagcgctccacggcctgcgccc 63
||||||||||||| |||||||||| | |||||| |||||||| ||| ||| |||||||||
Sbjct: 298 agtagggagtgcttacaccatttcctctccattcacatcagctctcgacg-cctgcgccc 356
Query: 64 ccggaattcccaaatcctgcggcgcctccgccccaaccccgctcgtcgactcctctcttc 123
|| ||||||||||| ||||| ||||||||||||||||| |||||||||||||||||| |
Sbjct: 357 cc--aattcccaaattctgcgccgcctccgccccaaccctgctcgtcgactcctctctgc 414
Query: 124 cgaacacgccggaggaatccttctccgcgactcgtcttcctcctctctgccgtgctggcg 183
||||||||||||||||||||||||||| |||||||||||||||||||||||| |||||||
Sbjct: 415 cgaacacgccggaggaatccttctccgagactcgtcttcctcctctctgccgggctggcg 474
Query: 184 tggagtggagatccaccaccacctggattggtctcttcttctgctggt 231
||||||||||||||| |||||| | |||||||||||||||||||||||
Sbjct: 475 tggagtggagatccatcaccacgtagattggtctcttcttctgctggt 522
>gb|CW415759.1|CW415759 fsbb001f116a03f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f116a03, DNA
sequence
Length = 344
Score = 274 bits (138), Expect = 1e-071
Identities = 205/225 (91%), Gaps = 3/225 (1%)
Strand = Plus / Plus
Query: 4 agtagggagtgctcacaccatttcttgtccattgacatcagcgctccacggcctgcgccc 63
||||||||||||| |||||||||| | |||||| |||||||| ||| ||| |||||||||
Sbjct: 123 agtagggagtgcttacaccatttcctctccattcacatcagctctcgacg-cctgcgccc 181
Query: 64 ccggaattcccaaatcctgcggcgcctccgccccaaccccgctcgtcgactcctctcttc 123
|| ||||||||||| ||||| ||||||||||||||||| |||||||||||||||||| |
Sbjct: 182 cc--aattcccaaattctgcgccgcctccgccccaaccctgctcgtcgactcctctctgc 239
Query: 124 cgaacacgccggaggaatccttctccgcgactcgtcttcctcctctctgccgtgctggcg 183
||||||| ||||||||||||||||||| |||||| ||||||||||||||||| |||||||
Sbjct: 240 cgaacactccggaggaatccttctccgagactcggcttcctcctctctgccgggctggcg 299
Query: 184 tggagtggagatccaccaccacctggattggtctcttcttctgct 228
||||||||||||||| |||||| | ||||||||||||||||||||
Sbjct: 300 tggagtggagatccatcaccacgtagattggtctcttcttctgct 344
>gb|BG464223.1|BG464223 EM1_71_B05.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 528
Score = 248 bits (125), Expect = 6e-064
Identities = 163/178 (91%)
Strand = Plus / Plus
Query: 528 acagcatatcctgattggtctcagttccaggcatactataatgctgctgggacagctcca 587
||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
Sbjct: 11 acagcatatcctgattggtctcagttccaggcatactataatgctcctgggacagctccc 70
Query: 588 gtaaccccaccggcnnnnnnncattcatctgtagctccaacccatcaggggcacccgtat 647
|| ||||||||||| ||||||||||||||||||| || |||||| ||||| |||
Sbjct: 71 gtgaccccaccggctttttttcattcatctgtagctccaagccctcagggacacccatat 130
Query: 648 atgtggggtccacagatgatgcctccttatgggacaccaccaccatatgcaacaatgt 705
||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
Sbjct: 131 atgtggggtccacagatgatgcctccttatgggacaccaccaccatatgcagcaatgt 188
>gb|BG649135.1|BG649135 EM1_56_A05.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 527
Score = 248 bits (125), Expect = 6e-064
Identities = 163/178 (91%)
Strand = Plus / Plus
Query: 528 acagcatatcctgattggtctcagttccaggcatactataatgctgctgggacagctcca 587
||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||
Sbjct: 11 acagcatatcctgattggtctcagttccaggcatactataatgctcctgggacagctccc 70
Query: 588 gtaaccccaccggcnnnnnnncattcatctgtagctccaacccatcaggggcacccgtat 647
|| ||||||||||| ||||||||||||||||||| || |||||| ||||| |||
Sbjct: 71 gtgaccccaccggctttttttcattcatctgtagctccaagccctcagggacacccatat 130
Query: 648 atgtggggtccacagatgatgcctccttatgggacaccaccaccatatgcaacaatgt 705
||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
Sbjct: 131 atgtggggtccacagatgatgcctccttatgggacaccaccaccatatgcagcaatgt 188
>gb|CW108361.1|CW108361 104_480_11097488_116_34496_032 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11097488, DNA
sequence
Length = 413
Score = 115 bits (58), Expect = 6e-024
Identities = 93/107 (86%)
Strand = Plus / Plus
Query: 556 aggcatactataatgctgctgggacagctccagtaaccccaccggcnnnnnnncattcat 615
||||||||||||||||| ||||||||||||| || ||||||||||| |||||||
Sbjct: 159 aggcatactataatgctcctgggacagctcccgtgaccccaccggctttttttcattcat 218
Query: 616 ctgtagctccaacccatcaggggcacccgtatatgtggggtccacag 662
|||||||||||| || |||||| ||||| ||||||||||||||||||
Sbjct: 219 ctgtagctccaagccctcagggacacccatatatgtggggtccacag 265
Score = 69.9 bits (35), Expect = 3e-010
Identities = 55/64 (85%)
Strand = Plus / Plus
Query: 495 cagaatgagccgagtacacctgctaannnnnnnacagcatatcctgattggtctcagttc 554
|||||||||| |||||||||| |||| |||||||||||||||||||||||||||
Sbjct: 13 cagaatgagcagagtacacctactaacccccccacagcatatcctgattggtctcagttc 72
Query: 555 cagg 558
||||
Sbjct: 73 cagg 76
>gb|CL164400.1|CL164400 104_358_10807882_114_31814_250 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10807882, DNA
sequence
Length = 624
Score = 42.1 bits (21), Expect = 0.068
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 178 ctggcgtggagtggagatcca 198
|||||||||||||||||||||
Sbjct: 299 ctggcgtggagtggagatcca 319
>gb|CW397078.1|CW397078 fsbb001f085n01f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f085n01, DNA
sequence
Length = 573
Score = 42.1 bits (21), Expect = 0.068
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 178 ctggcgtggagtggagatcca 198
|||||||||||||||||||||
Sbjct: 322 ctggcgtggagtggagatcca 342
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 207,161
Number of Sequences: 832831
Number of extensions: 207161
Number of successful extensions: 55491
Number of sequences better than 0.5: 10
Number of HSP's better than 0.5 without gapping: 10
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 55453
Number of HSP's gapped (non-prelim): 13
length of query: 705
length of database: 491,359,669
effective HSP length: 20
effective length of query: 685
effective length of database: 474,703,049
effective search space: 325171588565
effective search space used: 325171588565
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)