BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QAF14f01.yg.2.1
         (319 letters)

Database: Sorghum_nucl_with_EST.fasta 
           832,831 sequences; 491,359,669 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CL158360.1|CL158360  104_347_10803488_114_31377_080 Sorgh...   167   8e-040
gb|CL158361.1|CL158361  104_347_10803488_116_31378_080 Sorgh...   167   8e-040
gb|CW052847.1|CW052847  104_292_10515652_114_30286 Sorghum m...    38   0.47 
>gb|CL158360.1|CL158360 104_347_10803488_114_31377_080 Sorghum methylation-filtered library
           (LibID: 104) Sorghum bicolor genomic clone 10803488, DNA
           sequence
          Length = 727

 Score =  167 bits (84), Expect = 8e-040
 Identities = 90/93 (96%)
 Strand = Plus / Minus

                                                                       
Query: 21  cactgcagcactacggattgcctcagggctgatgcttggattatagccaagacccattac 80
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 589 cactgcagcactacggattgcctcagggctgatgcttggattatagccaagacccattac 530

                                            
Query: 81  atgccatgacttatccannngcttggtattgcc 113
           |||||||||||||||||   |||||||||||||
Sbjct: 529 atgccatgacttatccagtggcttggtattgcc 497

 Score = 61.9 bits (31), Expect = 3e-008
 Identities = 43/49 (87%)
 Strand = Plus / Minus

                                                            
Query: 129 cccagctgttagaatggatnnnggatcccacagctcnnnatcctcattc 177
           |||||||||||||||||||   ||||||||||||||   ||||||||||
Sbjct: 481 cccagctgttagaatggatgtaggatcccacagctcgccatcctcattc 433
>gb|CL158361.1|CL158361 104_347_10803488_116_31378_080 Sorghum methylation-filtered library
           (LibID: 104) Sorghum bicolor genomic clone 10803488, DNA
           sequence
          Length = 731

 Score =  167 bits (84), Expect = 8e-040
 Identities = 90/93 (96%)
 Strand = Plus / Plus

                                                                       
Query: 21  cactgcagcactacggattgcctcagggctgatgcttggattatagccaagacccattac 80
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 247 cactgcagcactacggattgcctcagggctgatgcttggattatagccaagacccattac 306

                                            
Query: 81  atgccatgacttatccannngcttggtattgcc 113
           |||||||||||||||||   |||||||||||||
Sbjct: 307 atgccatgacttatccagtggcttggtattgcc 339

 Score = 61.9 bits (31), Expect = 3e-008
 Identities = 43/49 (87%)
 Strand = Plus / Plus

                                                            
Query: 129 cccagctgttagaatggatnnnggatcccacagctcnnnatcctcattc 177
           |||||||||||||||||||   ||||||||||||||   ||||||||||
Sbjct: 355 cccagctgttagaatggatgtaggatcccacagctcgccatcctcattc 403
>gb|CW052847.1|CW052847 104_292_10515652_114_30286 Sorghum methylation filtered library
           (LibID: 104) Sorghum bicolor genomic clone 10515652, DNA
           sequence
          Length = 750

 Score = 38.2 bits (19), Expect = 0.47
 Identities = 19/19 (100%)
 Strand = Plus / Plus

                              
Query: 260 atgcacaggcattgggatt 278
           |||||||||||||||||||
Sbjct: 696 atgcacaggcattgggatt 714
  Database: Sorghum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:54 PM
  Number of letters in database: 491,359,669
  Number of sequences in database:  832,831
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 36,350
Number of Sequences: 832831
Number of extensions: 36350
Number of successful extensions: 8975
Number of sequences better than  0.5: 3
Number of HSP's better than  0.5 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 8970
Number of HSP's gapped (non-prelim): 5
length of query: 319
length of database: 491,359,669
effective HSP length: 19
effective length of query: 300
effective length of database: 475,535,880
effective search space: 142660764000
effective search space used: 142660764000
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)