BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QAF11h01.xg.2.1
         (328 letters)

Database: Sorghum_nucl_with_EST.fasta 
           832,831 sequences; 491,359,669 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CL158360.1|CL158360  104_347_10803488_114_31377_080 Sorgh...   295   1e-078
gb|CL158361.1|CL158361  104_347_10803488_116_31378_080 Sorgh...   295   1e-078
gb|CF675663.1|CF675663  MtN3 Subtractive cDNA library from s...    46   0.002
gb|DR831467.1|DR831467  MM71 Sorghum bicolor greenbug-respon...    46   0.002
gb|DV162814.1|DV162814  P1-N10 Sorghum bicolor greenbug-resp...    46   0.002
gb|DV162815.1|DV162815  P3-J15 Sorghum bicolor greenbug-resp...    46   0.002
gb|DR831427.1|DR831427  MT54 Sorghum bicolor greenbug-respon...    44   0.008
gb|DR831556.2|DR831556  MT75 Sorghum bicolor greenbug-respon...    44   0.008
gb|DR831428.1|DR831428  MM77 Sorghum bicolor greenbug-respon...    40   0.12 
gb|CW052847.1|CW052847  104_292_10515652_114_30286 Sorghum m...    38   0.48 
gb|CW144377.1|CW144377  104_536_11137911_148_34962_056 Sorgh...    38   0.48 
gb|CF487333.1|CF487333  POL1_43_D11.b1_A002 Pollen Sorghum b...    38   0.48 
>gb|CL158360.1|CL158360 104_347_10803488_114_31377_080 Sorghum methylation-filtered library
           (LibID: 104) Sorghum bicolor genomic clone 10803488, DNA
           sequence
          Length = 727

 Score =  295 bits (149), Expect = 1e-078
 Identities = 155/157 (98%)
 Strand = Plus / Minus

                                                                       
Query: 3   cactgcagcactacggattgcctcagggctgatgcttggattatagccaagacccattac 62
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 589 cactgcagcactacggattgcctcagggctgatgcttggattatagccaagacccattac 530

                                                                       
Query: 63  atgccatgacttatccagtggcttggtattgccgtaaaaggacatcagcccagctgttag 122
           ||||||||||||||||||||||||||||||||| || |||||||||||||||||||||||
Sbjct: 529 atgccatgacttatccagtggcttggtattgccatagaaggacatcagcccagctgttag 470

                                                
Query: 123 aatggatgtaggatcccacagctcgccatcctcattc 159
           |||||||||||||||||||||||||||||||||||||
Sbjct: 469 aatggatgtaggatcccacagctcgccatcctcattc 433
>gb|CL158361.1|CL158361 104_347_10803488_116_31378_080 Sorghum methylation-filtered library
           (LibID: 104) Sorghum bicolor genomic clone 10803488, DNA
           sequence
          Length = 731

 Score =  295 bits (149), Expect = 1e-078
 Identities = 155/157 (98%)
 Strand = Plus / Plus

                                                                       
Query: 3   cactgcagcactacggattgcctcagggctgatgcttggattatagccaagacccattac 62
           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 247 cactgcagcactacggattgcctcagggctgatgcttggattatagccaagacccattac 306

                                                                       
Query: 63  atgccatgacttatccagtggcttggtattgccgtaaaaggacatcagcccagctgttag 122
           ||||||||||||||||||||||||||||||||| || |||||||||||||||||||||||
Sbjct: 307 atgccatgacttatccagtggcttggtattgccatagaaggacatcagcccagctgttag 366

                                                
Query: 123 aatggatgtaggatcccacagctcgccatcctcattc 159
           |||||||||||||||||||||||||||||||||||||
Sbjct: 367 aatggatgtaggatcccacagctcgccatcctcattc 403
>gb|CF675663.1|CF675663 MtN3 Subtractive cDNA library from sorghum infested by greenbug
           aphids Sorghum bicolor cDNA clone MtN3 similar to
           MtN3-like protein, mRNA sequence
          Length = 194

 Score = 46.1 bits (23), Expect = 0.002
 Identities = 23/23 (100%)
 Strand = Plus / Plus

                                  
Query: 306 gtacctcggccgcgaccacgcta 328
           |||||||||||||||||||||||
Sbjct: 143 gtacctcggccgcgaccacgcta 165
>gb|DR831467.1|DR831467 MM71 Sorghum bicolor greenbug-responsive cDNA library Sorghum
           bicolor cDNA 5', mRNA sequence
          Length = 751

 Score = 46.1 bits (23), Expect = 0.002
 Identities = 23/23 (100%)
 Strand = Plus / Minus

                                  
Query: 306 gtacctcggccgcgaccacgcta 328
           |||||||||||||||||||||||
Sbjct: 127 gtacctcggccgcgaccacgcta 105
>gb|DV162814.1|DV162814 P1-N10 Sorghum bicolor greenbug-responsive cDNA library Sorghum
           bicolor cDNA 5', mRNA sequence
          Length = 198

 Score = 46.1 bits (23), Expect = 0.002
 Identities = 23/23 (100%)
 Strand = Plus / Plus

                                  
Query: 306 gtacctcggccgcgaccacgcta 328
           |||||||||||||||||||||||
Sbjct: 95  gtacctcggccgcgaccacgcta 117
>gb|DV162815.1|DV162815 P3-J15 Sorghum bicolor greenbug-responsive cDNA library Sorghum
           bicolor cDNA 5', mRNA sequence
          Length = 229

 Score = 46.1 bits (23), Expect = 0.002
 Identities = 23/23 (100%)
 Strand = Plus / Minus

                                  
Query: 306 gtacctcggccgcgaccacgcta 328
           |||||||||||||||||||||||
Sbjct: 40  gtacctcggccgcgaccacgcta 18
>gb|DR831427.1|DR831427 MT54 Sorghum bicolor greenbug-responsive cDNA library Sorghum
           bicolor cDNA 5', mRNA sequence
          Length = 199

 Score = 44.1 bits (22), Expect = 0.008
 Identities = 22/22 (100%)
 Strand = Plus / Minus

                                 
Query: 306 gtacctcggccgcgaccacgct 327
           ||||||||||||||||||||||
Sbjct: 22  gtacctcggccgcgaccacgct 1
>gb|DR831556.2|DR831556 MT75 Sorghum bicolor greenbug-responsive cDNA library Sorghum
           bicolor cDNA 5', mRNA sequence
          Length = 309

 Score = 44.1 bits (22), Expect = 0.008
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                 
Query: 306 gtacctcggccgcgaccacgct 327
           ||||||||||||||||||||||
Sbjct: 205 gtacctcggccgcgaccacgct 226
>gb|DR831428.1|DR831428 MM77 Sorghum bicolor greenbug-responsive cDNA library Sorghum
           bicolor cDNA 5', mRNA sequence
          Length = 299

 Score = 40.1 bits (20), Expect = 0.12
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                               
Query: 306 gtacctcggccgcgaccacg 325
           ||||||||||||||||||||
Sbjct: 22  gtacctcggccgcgaccacg 3
>gb|CW052847.1|CW052847 104_292_10515652_114_30286 Sorghum methylation filtered library
           (LibID: 104) Sorghum bicolor genomic clone 10515652, DNA
           sequence
          Length = 750

 Score = 38.2 bits (19), Expect = 0.48
 Identities = 19/19 (100%)
 Strand = Plus / Plus

                              
Query: 242 atgcacaggcattgggatt 260
           |||||||||||||||||||
Sbjct: 696 atgcacaggcattgggatt 714
>gb|CW144377.1|CW144377 104_536_11137911_148_34962_056 Sorghum methylation filtered library
           (LibID: 104) Sorghum bicolor genomic clone 11137911, DNA
           sequence
          Length = 622

 Score = 38.2 bits (19), Expect = 0.48
 Identities = 22/23 (95%)
 Strand = Plus / Minus

                                  
Query: 61  acatgccatgacttatccagtgg 83
           |||||||||||||| ||||||||
Sbjct: 372 acatgccatgacttgtccagtgg 350
>gb|CF487333.1|CF487333 POL1_43_D11.b1_A002 Pollen Sorghum bicolor cDNA clone
           POL1_43_D11_A002 3', mRNA sequence
          Length = 661

 Score = 38.2 bits (19), Expect = 0.48
 Identities = 22/23 (95%)
 Strand = Plus / Minus

                                  
Query: 61  acatgccatgacttatccagtgg 83
           |||||||||||||| ||||||||
Sbjct: 231 acatgccatgacttgtccagtgg 209
  Database: Sorghum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:54 PM
  Number of letters in database: 491,359,669
  Number of sequences in database:  832,831
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 67,511
Number of Sequences: 832831
Number of extensions: 67511
Number of successful extensions: 17567
Number of sequences better than  0.5: 12
Number of HSP's better than  0.5 without gapping: 12
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 17555
Number of HSP's gapped (non-prelim): 12
length of query: 328
length of database: 491,359,669
effective HSP length: 19
effective length of query: 309
effective length of database: 475,535,880
effective search space: 146940586920
effective search space used: 146940586920
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)