BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 5704580.2.1
(721 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CD208091.1|CD208091 HS1_31_E03.b1_A012 Heat-shocked seed... 876 0.0
gb|CD224742.1|CD224742 CCC1_36_C12.b1_A007 Callus culture/c... 698 0.0
gb|CW154734.1|CW154734 104_557_11145759_148_36356_064 Sorgh... 42 0.070
gb|CW190701.1|CW190701 104_612_11174645_148_36834_023 Sorgh... 40 0.27
>gb|CD208091.1|CD208091 HS1_31_E03.b1_A012 Heat-shocked seedlings Sorghum bicolor cDNA
clone HS1_31_E03_A012 3', mRNA sequence
Length = 591
Score = 876 bits (442), Expect = 0.0
Identities = 558/594 (93%), Gaps = 7/594 (1%)
Strand = Plus / Minus
Query: 18 ttttattgaataaattacatattcttacaccaaaatcctctgtagctaagcaaagtggaa 77
|||||||||||| ||||||| || |||||||||||| ||||||||||||| ||| |||||
Sbjct: 588 ttttattgaatagattacatgtttttacaccaaaattctctgtagctaagtaaaatggaa 529
Query: 78 tatacatttttgctcctcaagctgagtacacactgatagtctaaacgtgaaacccaacag 137
|||||| |||||||||| |||||| |||||||||||||| |||||||||||||||||||
Sbjct: 528 tatacacttttgctcctgaagctgcatacacactgatagtataaacgtgaaacccaacag 469
Query: 138 tagcatcgacaaaagaacaaaagaatgatcatagagaagtgaaacgtacagtctttgcct 197
|||||||||||||||||||||||||||||||| |||| ||||| ||||||||||| |||
Sbjct: 468 tagcatcgacaaaagaacaaaagaatgatcatttagaaatgaaatgtacagtcttt-cct 410
Query: 198 aggtggtacgtttgtccactctgct-caagtccacacttactgcccaagcttcctccata 256
|||| | ||||||||||||| || ||| ||||||||||||||| ||||||||||
Sbjct: 409 tggtg-----tctgtccactctgctgcatgtcaccacttactgcccaagtttcctccata 355
Query: 257 tgcctttctttgctgtcaagcacttcaatttgccaaacacaaaaccggacttctgagggt 316
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 354 tgcctttctttgctgtcaagcacttcaatttgccaaacacaaaaccggacttctgagggt 295
Query: 317 tgccatccacaagcgagttctcgtatatctctttgcattcttgtacaacctccttggctt 376
|||||||||| ||||| |||||||||||||||||||||||||||||||||||||| ||||
Sbjct: 294 tgccatccacgagcgaattctcgtatatctctttgcattcttgtacaacctccttagctt 235
Query: 377 ccttttttggaatgccttcccgcattgccacaagcttataaaaaacctcaggcgggcaat 436
||||| ||||||||||||||||||||||||||||||| | || |||||||||||||||||
Sbjct: 234 cctttcttggaatgccttcccgcattgccacaagcttctcaacaacctcaggcgggcaat 175
Query: 437 caggttgatgttccagtatattagtgtaaatgagagtaaagctatccaaactctcagctg 496
|||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
Sbjct: 174 caggttgatgttccagtatatttgtgtaaatgagagtaaagctatccaaactctcagctg 115
Query: 497 aagctagctccctcatatctgccagaatccgcactctgctttcaacttttgtcacgttga 556
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 114 aagctagctccctcatatctgccagaatccgcactctgctttcaacttttgtcacgttga 55
Query: 557 tgtgttccctgaagaaatccataagcacctcttcatctaacctcatcctctcta 610
||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 54 tgtgttccctgaagaaatccataagcacctcttcatctaacctcatcctctcta 1
>gb|CD224742.1|CD224742 CCC1_36_C12.b1_A007 Callus culture/cell suspension Sorghum bicolor
cDNA clone CCC1_36_C12_A007 3', mRNA sequence
Length = 522
Score = 698 bits (352), Expect = 0.0
Identities = 490/528 (92%), Gaps = 11/528 (2%)
Strand = Plus / Minus
Query: 24 tgaataaattacata-ttcttacaccaaaatcctc-tgtagctaagcaaagtggaatata 81
|||||||||||||| || |||||||||||| ||| |||||||||| ||| |||||||||
Sbjct: 522 tgaataaattacatggtttttacaccaaaattctcctgtagctaagtaaaatggaatata 463
Query: 82 cat-ttttgctcctcaagctgagtacacactg-atagtctaaacgtgaaacccaacagta 139
|| |||||||||| |||||| |||||||||| ||||| |||||||||||||||||||||
Sbjct: 462 caccttttgctcctgaagctgcgtacacactggatagtataaacgtgaaacccaacagta 403
Query: 140 gcatcgacaaaagaacaaaagaatgatcatagagaagtgaaacgtacagtctttgcctag 199
|||||||||||||||||||||||||||||| |||| ||||| ||||||||||| ||| |
Sbjct: 402 gcatcgacaaaagaacaaaagaatgatcatttagaaatgaaatgtacagtcttt-ccttg 344
Query: 200 gtggtacgtttgtccactctgct-caagtccacacttactgcccaagcttcctccatatg 258
||| | ||||||||||||| || ||| ||||||||||||||| ||||||||||||
Sbjct: 343 gtg-----tctgtccactctgctgcatgtcaccacttactgcccaagtttcctccatatg 289
Query: 259 cctttctttgctgtcaagcacttcaatttgccaaacacaaaaccggacttctgagggttg 318
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 288 cctttctttgctgtcaagcacttcaatttgccaaacacaaaaccggacttctgagggttg 229
Query: 319 ccatccacaagcgagttctcgtatatctctttgcattcttgtacaacctccttggcttcc 378
|||||||| ||||| |||||||||||||||||||||||||||||||||||||| ||||||
Sbjct: 228 ccatccacgagcgaattctcgtatatctctttgcattcttgtacaacctccttagcttcc 169
Query: 379 ttttttggaatgccttcccgcattgccacaagcttataaaaaacctcaggcgggcaatca 438
||| ||||||||||||||||||||||||||||||| | || |||||||||||||||||||
Sbjct: 168 tttcttggaatgccttcccgcattgccacaagcttctcaacaacctcaggcgggcaatca 109
Query: 439 ggttgatgttccagtatattagtgtaaatgagagtaaagctatccaaactctcagctgaa 498
|||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct: 108 ggttgatgttccagtatatttgtgtaaatgagagtaaagctatccaaactctcagctgaa 49
Query: 499 gctagctccctcatatctgccagaatccgcactctgctttcaactttt 546
||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 48 gctagctccctcatatctgccagaatccgcactctgctttcaactttt 1
>gb|CW154734.1|CW154734 104_557_11145759_148_36356_064 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11145759, DNA
sequence
Length = 706
Score = 42.1 bits (21), Expect = 0.070
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 618 ttcttttatgtagtttttctg 638
|||||||||||||||||||||
Sbjct: 382 ttcttttatgtagtttttctg 402
>gb|CW190701.1|CW190701 104_612_11174645_148_36834_023 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11174645, DNA
sequence
Length = 701
Score = 40.1 bits (20), Expect = 0.27
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 17 gttttattgaataaattaca 36
||||||||||||||||||||
Sbjct: 207 gttttattgaataaattaca 226
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 189,069
Number of Sequences: 832831
Number of extensions: 189069
Number of successful extensions: 51629
Number of sequences better than 0.5: 4
Number of HSP's better than 0.5 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 51618
Number of HSP's gapped (non-prelim): 6
length of query: 721
length of database: 491,359,669
effective HSP length: 20
effective length of query: 701
effective length of database: 474,703,049
effective search space: 332766837349
effective search space used: 332766837349
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)