BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3064721.2.2
(595 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF507006.1|BF507006 15782P-16 Pooled green leaf and root... 176 2e-042
gb|BF507175.1|BF507175 2604P-22 Pooled green leaf and root ... 170 9e-041
gb|BM329584.1|BM329584 PIC1_40_E09.g1_A002 Pathogen-infecte... 40 0.23
>gb|BF507006.1|BF507006 15782P-16 Pooled green leaf and root tissue Sorghum bicolor cDNA
clone 15782P-16, mRNA sequence
Length = 138
Score = 176 bits (89), Expect = 2e-042
Identities = 125/136 (91%), Gaps = 2/136 (1%)
Strand = Plus / Minus
Query: 289 ggtcagcacacgtcaattacgatcaagtgtttctacgggagtgcaacataaacccctgaa 348
|||||||| ||||||||||||||||||||||||||||||||||||| |||||||| ||||
Sbjct: 138 ggtcagcatacgtcaattacgatcaagtgtttctacgggagtgcaatataaacccgtgaa 79
Query: 349 cagcaaatagtggtgcatggtttatatccttttcttttcaatgatttcttagaccaattc 408
|||||||||||||| |||| | ||||||||||||||| || |||||||||||| ||||
Sbjct: 78 cagcaaatagtggtctatgg--tgtatccttttcttttcgattatttcttagaccgattc 21
Query: 409 tttgtgctctaggtaa 424
||||||||||||||||
Sbjct: 20 tttgtgctctaggtaa 5
>gb|BF507175.1|BF507175 2604P-22 Pooled green leaf and root tissue Sorghum bicolor cDNA
clone 2604P-22, mRNA sequence
Length = 142
Score = 170 bits (86), Expect = 9e-041
Identities = 114/122 (93%), Gaps = 1/122 (0%)
Strand = Plus / Minus
Query: 464 ctttcctgatgtaaaggtgccagcaggttggcggtaggcttggatcatacaaacagttag 523
||||||||||||||||||||||||||||||| ||||||||| ||||||||||||||||||
Sbjct: 142 ctttcctgatgtaaaggtgccagcaggttggtggtaggcttagatcatacaaacagttag 83
Query: 524 tagccaaaggcatatcccgttttgtgcttataaatgctaatatggtagataatttttgac 583
||||| ||||| ||| | |||||||||||||||||||||||| ||||||||||||||||
Sbjct: 82 tagcc-aaggcgtatttcattttgtgcttataaatgctaatatagtagataatttttgac 24
Query: 584 tg 585
||
Sbjct: 23 tg 22
>gb|BM329584.1|BM329584 PIC1_40_E09.g1_A002 Pathogen-infected compatible 1 (PIC1) Sorghum
bicolor cDNA, mRNA sequence
Length = 628
Score = 40.1 bits (20), Expect = 0.23
Identities = 38/44 (86%)
Strand = Plus / Plus
Query: 222 gtcatccactacaacggaaacctgaagccctggcttgagattgg 265
||||| || ||||| || ||| ||||||||||||| ||||||||
Sbjct: 162 gtcatacattacaatggcaacatgaagccctggctagagattgg 205
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 138,247
Number of Sequences: 832831
Number of extensions: 138247
Number of successful extensions: 38124
Number of sequences better than 0.5: 3
Number of HSP's better than 0.5 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 38119
Number of HSP's gapped (non-prelim): 3
length of query: 595
length of database: 491,359,669
effective HSP length: 19
effective length of query: 576
effective length of database: 475,535,880
effective search space: 273908666880
effective search space used: 273908666880
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)