BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 2542042.2.1
         (691 letters)

Database: Sorghum_nucl_with_EST.fasta 
           832,831 sequences; 491,359,669 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CW165269.1|CW165269  104_573_11152417_116_36472_005 Sorgh...   256   2e-066
gb|CW020916.1|CW020916  104_109_10409239_116_30501 Sorghum m...   107   1e-021
gb|CW020915.1|CW020915  104_109_10409239_114_30493 Sorghum m...   100   3e-019
>gb|CW165269.1|CW165269 104_573_11152417_116_36472_005 Sorghum methylation filtered library
           (LibID: 104) Sorghum bicolor genomic clone 11152417, DNA
           sequence
          Length = 571

 Score =  256 bits (129), Expect = 2e-066
 Identities = 237/273 (86%), Gaps = 3/273 (1%)
 Strand = Plus / Minus

                                                                       
Query: 39  cctgcgccacctccgagcaggcggcggccgacttcgctgcctcctcgctcggggtcaggg 98
           |||||||||||||||||||||||||| |||||||||||||| ||||||| |||||||| |
Sbjct: 481 cctgcgccacctccgagcaggcggcgaccgacttcgctgccgcctcgctgggggtcagtg 422

                                                                       
Query: 99  ccagcaagctggtcgcgctcgtcaccaccgtccatggggggaaggacgccactagatacg 158
           ||||| ||||||||||  |||||||   |||||| ||| |  |||||||| | || ||||
Sbjct: 421 ccagcgagctggtcgccgtcgtcacngtcgtccangggagctaggacgccgccaggtacg 362

                                                                       
Query: 159 tggtggcgcccaacggcatcacacgcatcggcaaggcggcaggggccgcggccgtagtgc 218
           ||||||||||  ||||| || | |||||||||||||||   || ||||| ||||  ||||
Sbjct: 361 tggtggcgccagacggcgtcgcgcgcatcggcaaggcg---ggagccgccgccgcggtgc 305

                                                                       
Query: 219 cgtgccacccgatgtcgtacccgtacatggtccactactgccaccagccggcggacgtgg 278
           |||||||||| ||| |||||||||||||||||||||||||||||| ||||||||||||||
Sbjct: 304 cgtgccaccctatggcgtacccgtacatggtccactactgccaccggccggcggacgtgg 245

                                            
Query: 279 aggcgctccgtattgagctgaccgggctcggag 311
           ||||||||||  | |||||||| ||||||||||
Sbjct: 244 aggcgctccgcgtcgagctgacggggctcggag 212

 Score =  155 bits (78), Expect = 6e-036
 Identities = 120/134 (89%)
 Strand = Plus / Minus

                                                                       
Query: 326 accgcgatcgccatgtgccacgccaacaccatgaactgggacgatcgctacttccaaatg 385
           ||||||||||||||||||||||||||||||| || ||||||||  || |||||| | |||
Sbjct: 179 accgcgatcgccatgtgccacgccaacaccacgagctgggacgcccgatacttcgagatg 120

                                                                       
Query: 386 ctgaacgtgacgcgcggcgaggagatctgccacttcatgccgcgcaactatgtgctctgg 445
           ||||||| ||||||||| |||||||||||||||||||||||||| ||||| ||||| |||
Sbjct: 119 ctgaacgcgacgcgcggggaggagatctgccacttcatgccgcggaactacgtgctgtgg 60

                         
Query: 446 ctaccagctgccga 459
           || || ||||||||
Sbjct: 59  ctgccggctgccga 46
>gb|CW020916.1|CW020916 104_109_10409239_116_30501 Sorghum methylation filtered library
           (LibID: 104) Sorghum bicolor genomic clone 10409239, DNA
           sequence
          Length = 634

 Score =  107 bits (54), Expect = 1e-021
 Identities = 69/74 (93%)
 Strand = Plus / Plus

                                                                       
Query: 39  cctgcgccacctccgagcaggcggcggccgacttcgctgcctcctcgctcggggtcaggg 98
           |||||||||||||||||||||||||| |||||||||||||| ||||||| |||||||| |
Sbjct: 561 cctgcgccacctccgagcaggcggcgaccgacttcgctgccgcctcgctgggggtcagtg 620

                         
Query: 99  ccagcaagctggtc 112
           ||||| ||||||||
Sbjct: 621 ccagcgagctggtc 634
>gb|CW020915.1|CW020915 104_109_10409239_114_30493 Sorghum methylation filtered library
           (LibID: 104) Sorghum bicolor genomic clone 10409239, DNA
           sequence
          Length = 717

 Score = 99.6 bits (50), Expect = 3e-019
 Identities = 77/86 (89%)
 Strand = Plus / Minus

                                                                       
Query: 374 tacttccaaatgctgaacgtgacgcgcggcgaggagatctgccacttcatgccgcgcaac 433
           |||||| | |||||||||| ||||||||| |||||||||||||||||||||||||| |||
Sbjct: 708 tacttcgagatgctgaacgcgacgcgcggggaggagatctgccacttcatgccgcggaac 649

                                     
Query: 434 tatgtgctctggctaccagctgccga 459
           || ||||| ||||| || ||||||||
Sbjct: 648 tacgtgctgtggctgccggctgccga 623
  Database: Sorghum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:54 PM
  Number of letters in database: 491,359,669
  Number of sequences in database:  832,831
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 193,772
Number of Sequences: 832831
Number of extensions: 193772
Number of successful extensions: 53839
Number of sequences better than  0.5: 3
Number of HSP's better than  0.5 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 53832
Number of HSP's gapped (non-prelim): 6
length of query: 691
length of database: 491,359,669
effective HSP length: 20
effective length of query: 671
effective length of database: 474,703,049
effective search space: 318525745879
effective search space used: 318525745879
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)