BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2448655.2.1
(682 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CF489853.1|CF489853 POL1_61_B06.b1_A002 Pollen Sorghum b... 801 0.0
gb|CN135994.1|CN135994 OX1_40_D12.b1_A002 Oxidatively-stres... 557 e-157
gb|CF489936.1|CF489936 POL1_61_B06.g1_A002 Pollen Sorghum b... 313 1e-083
gb|CW032150.1|CW032150 104_261_10501217_114_30363 Sorghum m... 297 7e-079
gb|CW280756.1|CW280756 104_756_11407799_116_35449_094 Sorgh... 297 7e-079
gb|CN136077.1|CN136077 OX1_40_D12.g1_A002 Oxidatively-stres... 153 3e-035
gb|CW407060.1|CW407060 fsbb001f100f21f0 Sorghum methylation... 92 8e-017
gb|CW407061.1|CW407061 fsbb001f100f21k0 Sorghum methylation... 92 8e-017
gb|BZ352082.1|BZ352082 hm82b04.g1 WGS-SbicolorF (JM107 adap... 42 0.066
gb|CW275769.1|CW275769 104_749_11405136_116_35389_094 Sorgh... 42 0.066
gb|CW275770.1|CW275770 104_749_11405136_148_35393_094 Sorgh... 42 0.066
gb|BG463532.1|BG463532 EM1_49_F10.g1_A002 Embryo 1 (EM1) So... 42 0.066
gb|CL186121.1|CL186121 104_400_10900000_114_32416_052 Sorgh... 40 0.26
gb|CL186122.1|CL186122 104_400_10900000_116_32403_052 Sorgh... 40 0.26
gb|CW079405.1|CW079405 104_400_10900000_114_32399_052 Sorgh... 40 0.26
gb|CW326730.1|CW326730 104_822_11478372_116_35964_046 Sorgh... 40 0.26
>gb|CF489853.1|CF489853 POL1_61_B06.b1_A002 Pollen Sorghum bicolor cDNA clone
POL1_61_B06_A002 3', mRNA sequence
Length = 653
Score = 801 bits (404), Expect = 0.0
Identities = 470/492 (95%)
Strand = Plus / Plus
Query: 8 catgagttcactgcgccaataggattcgcagctccatatgcacactgggctgaggtgctg 67
||||||||||| |||||||| ||||||||||||||||||||||||||||||||||||||
Sbjct: 1 catgagttcaccgcgccaatcggattcgcagctccatatgcacactgggctgaggtgctc 60
Query: 68 atactcggcatcccctccttcgtcgggccagccattgttccaggccacatgatcacattc 127
||||| ||||||||||||||||||||||| ||||||| ||||||||||||||||||||||
Sbjct: 61 atacttggcatcccctccttcgtcgggccggccattgctccaggccacatgatcacattc 120
Query: 128 tggctctggattatactccgtcaggtggaggctatcgagacacatagcggctttgatttc 187
||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
Sbjct: 121 tggctctggattatactccgtcaggtggaggctatcgagacacatagtggctttgatttc 180
Query: 188 ccattcaccccgacaaagtatattccattctatggaggagcagaataccatgactatcat 247
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 ccattcaccccgacaaagtatattccattctatggaggagcagaataccatgactatcat 240
Query: 248 cattatgtaggaggccagagccaaagcaattttgcttctgttttcacttactgtgattac 307
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 241 cattatgtaggaggccagagccaaagcaattttgcttctgttttcacttactgtgattac 300
Query: 308 ttatatggcactgacaaaggttacagattccacaaaacatacctagcaaagctgaaggat 367
|||||||| ||||| | ||||||||||||||||||| ||| |||||||||||||||||||
Sbjct: 301 ttatatgggactgatagaggttacagattccacaaagcatccctagcaaagctgaaggat 360
Query: 368 ctggggcataatgatggcgagaaaggagacggcagcggacccagctatgtgaaactggat 427
|||||||| |||||||||||||||||||| ||||| |||| ||||||||||||| |||||
Sbjct: 361 ctggggcaaaatgatggcgagaaaggagatggcagtggactcagctatgtgaaattggat 420
Query: 428 tagattgcaagatatagtgcatcttgagtgtttctatggtcatcgacttcactgtggcat 487
|||||||||||||| ||||||||||||||||||||||| |||| |||||||||||||||
Sbjct: 421 tagattgcaagatacagtgcatcttgagtgtttctatgaccatccacttcactgtggcat 480
Query: 488 ttcgaaatggga 499
|| |||||||||
Sbjct: 481 tttgaaatggga 492
Score = 75.8 bits (38), Expect = 5e-012
Identities = 53/58 (91%)
Strand = Plus / Plus
Query: 586 ctagcgtttatgaaattttatggaacttgtacgctgacgaataacaacgcaactctca 643
|||| |||| ||||||| ||||||||||||||||||||||||||||| |||||||||
Sbjct: 549 ctagtgtttgtgaaattccatggaacttgtacgctgacgaataacaacacaactctca 606
>gb|CN135994.1|CN135994 OX1_40_D12.b1_A002 Oxidatively-stressed leaves and roots Sorghum
bicolor cDNA clone OX1_40_D12_A002 3', mRNA sequence
Length = 772
Score = 557 bits (281), Expect = e-157
Identities = 329/345 (95%)
Strand = Plus / Plus
Query: 155 gaggctatcgagacacatagcggctttgatttcccattcaccccgacaaagtatattcca 214
|||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct: 1 gaggctatcgagacacatagtggctttgatttcccattcaccccgacaaagtatattcca 60
Query: 215 ttctatggaggagcagaataccatgactatcatcattatgtaggaggccagagccaaagc 274
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61 ttctatggaggagcagaataccatgactatcatcattatgtaggaggccagagccaaagc 120
Query: 275 aattttgcttctgttttcacttactgtgattacttatatggcactgacaaaggttacaga 334
|||||||||||||||||| |||||||||||||||||||||| ||||| | ||||||||||
Sbjct: 121 aattttgcttctgttttctcttactgtgattacttatatgggactgatagaggttacaga 180
Query: 335 ttccacaaaacatacctagcaaagctgaaggatctggggcataatgatggcgagaaagga 394
||||||||| ||||||||||||||||||||||||||||||| ||||||||||||||||||
Sbjct: 181 ttccacaaagcatacctagcaaagctgaaggatctggggcaaaatgatggcgagaaagga 240
Query: 395 gacggcagcggacccagctatgtgaaactggattagattgcaagatatagtgcatcttga 454
|| ||||| |||| ||||||||||||| ||||||||||||||||||| ||||||||||||
Sbjct: 241 gatggcagtggactcagctatgtgaaattggattagattgcaagatacagtgcatcttga 300
Query: 455 gtgtttctatggtcatcgacttcactgtggcatttcgaaatggga 499
||||||||||| |||| ||||||||||||||||| |||||||||
Sbjct: 301 gtgtttctatgaccatccacttcactgtggcattttgaaatggga 345
Score = 75.8 bits (38), Expect = 5e-012
Identities = 53/58 (91%)
Strand = Plus / Plus
Query: 586 ctagcgtttatgaaattttatggaacttgtacgctgacgaataacaacgcaactctca 643
|||| |||| ||||||| ||||||||||||||||||||||||||||| |||||||||
Sbjct: 402 ctagtgtttgtgaaattccatggaacttgtacgctgacgaataacaacacaactctca 459
>gb|CF489936.1|CF489936 POL1_61_B06.g1_A002 Pollen Sorghum bicolor cDNA clone
POL1_61_B06_A002 5', mRNA sequence
Length = 741
Score = 313 bits (158), Expect = 1e-083
Identities = 179/186 (96%)
Strand = Plus / Plus
Query: 5 caccatgagttcactgcgccaataggattcgcagctccatatgcacactgggctgaggtg 64
|||||||||||||| |||||||| ||||||||||||||||||||||||||||||||||||
Sbjct: 556 caccatgagttcaccgcgccaatcggattcgcagctccatatgcacactgggctgaggtg 615
Query: 65 ctgatactcggcatcccctccttcgtcgggccagccattgttccaggccacatgatcaca 124
|| ||||| ||||||||||||||||||||||| ||||||| |||||||||||||||||||
Sbjct: 616 ctcatacttggcatcccctccttcgtcgggccggccattgctccaggccacatgatcaca 675
Query: 125 ttctggctctggattatactccgtcaggtggaggctatcgagacacatagcggctttgat 184
|||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
Sbjct: 676 ttctggctctggattatactccgtcaggtggaggctatcgagacacatagtggctttgat 735
Query: 185 ttccca 190
||||||
Sbjct: 736 ttccca 741
>gb|CW032150.1|CW032150 104_261_10501217_114_30363 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 10501217, DNA
sequence
Length = 406
Score = 297 bits (150), Expect = 7e-079
Identities = 165/170 (97%)
Strand = Plus / Plus
Query: 5 caccatgagttcactgcgccaataggattcgcagctccatatgcacactgggctgaggtg 64
|||||||||||||| |||||||| ||||||||||||||||||||||||||||||||||||
Sbjct: 82 caccatgagttcaccgcgccaatcggattcgcagctccatatgcacactgggctgaggtg 141
Query: 65 ctgatactcggcatcccctccttcgtcgggccagccattgttccaggccacatgatcaca 124
|| ||||||||||||||||||||||||||||| ||||||| |||||||||||||||||||
Sbjct: 142 ctcatactcggcatcccctccttcgtcgggccggccattgctccaggccacatgatcaca 201
Query: 125 ttctggctctggattatactccgtcaggtggaggctatcgagacacatag 174
||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 202 ttctggctctggattatactccgtcaggtggaggctatcgagacacatag 251
>gb|CW280756.1|CW280756 104_756_11407799_116_35449_094 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11407799, DNA
sequence
Length = 350
Score = 297 bits (150), Expect = 7e-079
Identities = 165/170 (97%)
Strand = Plus / Minus
Query: 5 caccatgagttcactgcgccaataggattcgcagctccatatgcacactgggctgaggtg 64
|||||||||||||| |||||||| ||||||||||||||||||||||||||||||||||||
Sbjct: 280 caccatgagttcaccgcgccaatcggattcgcagctccatatgcacactgggctgaggtg 221
Query: 65 ctgatactcggcatcccctccttcgtcgggccagccattgttccaggccacatgatcaca 124
|| ||||||||||||||||||||||||||||| ||||||| |||||||||||||||||||
Sbjct: 220 ctcatactcggcatcccctccttcgtcgggccggccattgctccaggccacatgatcaca 161
Query: 125 ttctggctctggattatactccgtcaggtggaggctatcgagacacatag 174
||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 160 ttctggctctggattatactccgtcaggtggaggctatcgagacacatag 111
>gb|CN136077.1|CN136077 OX1_40_D12.g1_A002 Oxidatively-stressed leaves and roots Sorghum
bicolor cDNA clone OX1_40_D12_A002 5', mRNA sequence
Length = 738
Score = 153 bits (77), Expect = 3e-035
Identities = 86/89 (96%)
Strand = Plus / Plus
Query: 5 caccatgagttcactgcgccaataggattcgcagctccatatgcacactgggctgaggtg 64
|||||||||||||| |||||||| ||||||||||||||||||||||||||||||||||||
Sbjct: 650 caccatgagttcaccgcgccaatcggattcgcagctccatatgcacactgggctgaggtg 709
Query: 65 ctgatactcggcatcccctccttcgtcgg 93
|| ||||||||||||||||||||||||||
Sbjct: 710 ctcatactcggcatcccctccttcgtcgg 738
>gb|CW407060.1|CW407060 fsbb001f100f21f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f100f21, DNA
sequence
Length = 656
Score = 91.7 bits (46), Expect = 8e-017
Identities = 85/98 (86%)
Strand = Plus / Minus
Query: 209 attccattctatggaggagcagaataccatgactatcatcattatgtaggaggccagagc 268
||||||||||||||||| |||| |||||||||||||||||| || | |||||||| ||
Sbjct: 487 attccattctatggaggggcagcataccatgactatcatcactacgcaggaggccgtagt 428
Query: 269 caaagcaattttgcttctgttttcacttactgtgatta 306
|||||||| |||||| || ||||||| ||||| |||||
Sbjct: 427 caaagcaactttgctcctcttttcacctactgcgatta 390
>gb|CW407061.1|CW407061 fsbb001f100f21k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f100f21, DNA
sequence
Length = 808
Score = 91.7 bits (46), Expect = 8e-017
Identities = 85/98 (86%)
Strand = Plus / Plus
Query: 209 attccattctatggaggagcagaataccatgactatcatcattatgtaggaggccagagc 268
||||||||||||||||| |||| |||||||||||||||||| || | |||||||| ||
Sbjct: 474 attccattctatggaggggcagcataccatgactatcatcactacgcaggaggccgtagt 533
Query: 269 caaagcaattttgcttctgttttcacttactgtgatta 306
|||||||| |||||| || ||||||| ||||| |||||
Sbjct: 534 caaagcaactttgctcctcttttcacctactgcgatta 571
>gb|BZ352082.1|BZ352082 hm82b04.g1 WGS-SbicolorF (JM107 adapted methyl filtered) Sorghum
bicolor genomic clone hm82b04 5', DNA sequence
Length = 647
Score = 42.1 bits (21), Expect = 0.066
Identities = 27/29 (93%)
Strand = Plus / Minus
Query: 221 ggaggagcagaataccatgactatcatca 249
||||||||||||| ||||||||| |||||
Sbjct: 146 ggaggagcagaattccatgactaccatca 118
>gb|CW275769.1|CW275769 104_749_11405136_116_35389_094 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11405136, DNA
sequence
Length = 679
Score = 42.1 bits (21), Expect = 0.066
Identities = 27/29 (93%)
Strand = Plus / Minus
Query: 221 ggaggagcagaataccatgactatcatca 249
||||||||||||| ||||||||| |||||
Sbjct: 624 ggaggagcagaattccatgactaccatca 596
>gb|CW275770.1|CW275770 104_749_11405136_148_35393_094 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11405136, DNA
sequence
Length = 683
Score = 42.1 bits (21), Expect = 0.066
Identities = 27/29 (93%)
Strand = Plus / Plus
Query: 221 ggaggagcagaataccatgactatcatca 249
||||||||||||| ||||||||| |||||
Sbjct: 461 ggaggagcagaattccatgactaccatca 489
>gb|BG463532.1|BG463532 EM1_49_F10.g1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 565
Score = 42.1 bits (21), Expect = 0.066
Identities = 27/29 (93%)
Strand = Plus / Plus
Query: 221 ggaggagcagaataccatgactatcatca 249
||||||||||||| ||||||||| |||||
Sbjct: 78 ggaggagcagaattccatgactaccatca 106
>gb|CL186121.1|CL186121 104_400_10900000_114_32416_052 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10900000, DNA
sequence
Length = 648
Score = 40.1 bits (20), Expect = 0.26
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 248 cattatgtaggaggccagag 267
||||||||||||||||||||
Sbjct: 374 cattatgtaggaggccagag 355
>gb|CL186122.1|CL186122 104_400_10900000_116_32403_052 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10900000, DNA
sequence
Length = 628
Score = 40.1 bits (20), Expect = 0.26
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 248 cattatgtaggaggccagag 267
||||||||||||||||||||
Sbjct: 401 cattatgtaggaggccagag 420
>gb|CW079405.1|CW079405 104_400_10900000_114_32399_052 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 10900000, DNA
sequence
Length = 438
Score = 40.1 bits (20), Expect = 0.26
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 248 cattatgtaggaggccagag 267
||||||||||||||||||||
Sbjct: 373 cattatgtaggaggccagag 354
>gb|CW326730.1|CW326730 104_822_11478372_116_35964_046 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11478372, DNA
sequence
Length = 609
Score = 40.1 bits (20), Expect = 0.26
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 248 cattatgtaggaggccagag 267
||||||||||||||||||||
Sbjct: 35 cattatgtaggaggccagag 16
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 140,025
Number of Sequences: 832831
Number of extensions: 140025
Number of successful extensions: 35731
Number of sequences better than 0.5: 16
Number of HSP's better than 0.5 without gapping: 16
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 35707
Number of HSP's gapped (non-prelim): 24
length of query: 682
length of database: 491,359,669
effective HSP length: 19
effective length of query: 663
effective length of database: 475,535,880
effective search space: 315280288440
effective search space used: 315280288440
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)