BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCM3d10.yg.2.1
(683 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA924040.1|CA924040 MTU7CL.P1.A07 Aspen leaf cDNA Librar... 62 3e-008
gb|DN500621.1|DN500621 UL69F06.5pR Populus cold stressed le... 62 3e-008
>gb|CA924040.1|CA924040 MTU7CL.P1.A07 Aspen leaf cDNA Library Populus tremuloides cDNA,
mRNA sequence
Length = 700
Score = 61.9 bits (31), Expect = 3e-008
Identities = 121/151 (80%)
Strand = Plus / Minus
Query: 427 attgataaatatgatctatatggacaagtggcataccccaagcaccataagcaatctgaa 486
||||| || |||||||| ||||| ||||| |||||||| || || || ||||| |||||
Sbjct: 212 attgacaagtatgatctgtatgggcaagttgcataccctaaacatcacaagcagtctgat 153
Query: 487 gttcctgatatttatcgtttagctgcgagaacaaaaggagtttttatcaattgtgcattg 546
|||||||| |||||||| |||| |||| ||||| || ||||| |||||| || ||
Sbjct: 152 gttcctgacatttatcgactagcagcgaagacaaagggtgttttcatcaatccggctttc 93
Query: 547 gttgaaccatttggactcaccttgattgagg 577
|||| ||||||||||| || || |||||||
Sbjct: 92 attgagccatttggacttactttaattgagg 62
Score = 54.0 bits (27), Expect = 7e-006
Identities = 144/183 (78%)
Strand = Plus / Minus
Query: 355 atggggaatcgtgatgtcattgatgaaatgtcaagcacaaatgcagctgttttgacttca 414
||||| ||||| |||| |||||||||||||| ||||||| | || |||| | |||
Sbjct: 284 atgggcaatcgagatggaattgatgaaatgtccagcacaagttcatctgtccttctttct 225
Query: 415 gcactcaagttaattgataaatatgatctatatggacaagtggcataccccaagcaccat 474
| || ||| | ||||| || |||||||| ||||| ||||| |||||||| || || ||
Sbjct: 224 gtgcttaagcttattgacaagtatgatctgtatgggcaagttgcataccctaaacatcac 165
Query: 475 aagcaatctgaagttcctgatatttatcgtttagctgcgagaacaaaaggagtttttatc 534
||||| ||||| |||||||| |||||||| |||| |||| ||||| || ||||| |||
Sbjct: 164 aagcagtctgatgttcctgacatttatcgactagcagcgaagacaaagggtgttttcatc 105
Query: 535 aat 537
|||
Sbjct: 104 aat 102
>gb|DN500621.1|DN500621 UL69F06.5pR Populus cold stressed leaves cDNA library Populus
tremula x Populus tremuloides cDNA clone UL69F06 5',
mRNA sequence
Length = 789
Score = 61.9 bits (31), Expect = 3e-008
Identities = 121/151 (80%)
Strand = Plus / Plus
Query: 427 attgataaatatgatctatatggacaagtggcataccccaagcaccataagcaatctgaa 486
||||| || |||||||| ||||| ||||| |||||||| || || || ||||| |||||
Sbjct: 265 attgacaagtatgatctgtatgggcaagttgcataccctaaacatcacaagcagtctgat 324
Query: 487 gttcctgatatttatcgtttagctgcgagaacaaaaggagtttttatcaattgtgcattg 546
|||||||| |||||||| |||| |||| ||||| || ||||| |||||| || ||
Sbjct: 325 gttcctgacatttatcgactagcagcgaagacaaagggtgttttcatcaatccggctttc 384
Query: 547 gttgaaccatttggactcaccttgattgagg 577
|||| ||||||||||| || || |||||||
Sbjct: 385 attgagccatttggacttactttaattgagg 415
Score = 54.0 bits (27), Expect = 7e-006
Identities = 144/183 (78%)
Strand = Plus / Plus
Query: 355 atggggaatcgtgatgtcattgatgaaatgtcaagcacaaatgcagctgttttgacttca 414
||||| ||||| |||| |||||||||||||| ||||||| | || |||| | |||
Sbjct: 193 atgggcaatcgagatggaattgatgaaatgtccagcacaagttcatctgtccttctttct 252
Query: 415 gcactcaagttaattgataaatatgatctatatggacaagtggcataccccaagcaccat 474
| || ||| | ||||| || |||||||| ||||| ||||| |||||||| || || ||
Sbjct: 253 gtgcttaagcttattgacaagtatgatctgtatgggcaagttgcataccctaaacatcac 312
Query: 475 aagcaatctgaagttcctgatatttatcgtttagctgcgagaacaaaaggagtttttatc 534
||||| ||||| |||||||| |||||||| |||| |||| ||||| || ||||| |||
Sbjct: 313 aagcagtctgatgttcctgacatttatcgactagcagcgaagacaaagggtgttttcatc 372
Query: 535 aat 537
|||
Sbjct: 373 aat 375
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 78,995
Number of Sequences: 369679
Number of extensions: 78995
Number of successful extensions: 20714
Number of sequences better than 0.5: 2
Number of HSP's better than 0.5 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 20708
Number of HSP's gapped (non-prelim): 6
length of query: 683
length of database: 203,408,664
effective HSP length: 19
effective length of query: 664
effective length of database: 196,384,763
effective search space: 130399482632
effective search space used: 130399482632
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)