BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF1g01.yg.2.1
(335 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA821982.1|CA821982 R02C12 two-month-old roots from clon... 58 2e-007
gb|BP925807.1|BP925807 BP925807 full-length enriched poplar... 46 8e-004
gb|AC149540.1| Populus trichocarpa clone Pop1-111J19, compl... 46 8e-004
gb|BP924307.1|BP924307 BP924307 full-length enriched poplar... 44 0.003
>gb|CA821982.1|CA821982 R02C12 two-month-old roots from clone 'Beaupre' Populus trichocarpa
x Populus deltoides cDNA 5', mRNA sequence
Length = 291
Score = 58.0 bits (29), Expect = 2e-007
Identities = 92/113 (81%)
Strand = Plus / Minus
Query: 1 acacatctcggctgagaacttgctcaccattgcctccagcaactttcccctcaaatagtg 60
||||||| | ||| ||||||||||| ||||| ||||| || |||||||| ||||| || |
Sbjct: 233 acacatccctgcttagaacttgctcgccatttcctcctgccactttcccttcaaaaaggg 174
Query: 61 caatttggttcaagccaacatcacgccctttccccacctggatatattcatgg 113
|||| ||||| || ||||| || | || || || ||||| ||||||||||||
Sbjct: 173 caatctggtttaacccaacgtctcttccctttccaacctgaatatattcatgg 121
>gb|BP925807.1|BP925807 BP925807 full-length enriched poplar cDNA library Populus nigra
cDNA clone PnFL1-050_P13.f 5', mRNA sequence
Length = 365
Score = 46.1 bits (23), Expect = 8e-004
Identities = 65/79 (82%)
Strand = Plus / Minus
Query: 1 acacatctcggctgagaacttgctcaccattgcctccagcaactttcccctcaaatagtg 60
||||||| | ||| ||||||||||| ||||| ||||| || ||| |||| ||||| || |
Sbjct: 103 acacatccctgcttagaacttgctcgccatttcctcctgccactctcccttcaaaaaggg 44
Query: 61 caatttggttcaagccaac 79
|||| ||||| || |||||
Sbjct: 43 caatctggtttaacccaac 25
>gb|AC149540.1| Populus trichocarpa clone Pop1-111J19, complete sequence
Length = 96953
Score = 46.1 bits (23), Expect = 8e-004
Identities = 38/43 (88%)
Strand = Plus / Minus
Query: 15 agaacttgctcaccattgcctccagcaactttcccctcaaata 57
||||||||||||||||| || |||| ||| ||||| |||||||
Sbjct: 26099 agaacttgctcaccattaccaccagaaaccttcccttcaaata 26057
>gb|BP924307.1|BP924307 BP924307 full-length enriched poplar cDNA library Populus nigra
cDNA clone PnFL1-030_I23.f 5', mRNA sequence
Length = 342
Score = 44.1 bits (22), Expect = 0.003
Identities = 58/70 (82%)
Strand = Plus / Minus
Query: 1 acacatctcggctgagaacttgctcaccattgcctccagcaactttcccctcaaatagtg 60
||||||| | ||| ||||||||||| ||||| |||| || |||||||| ||||| || |
Sbjct: 76 acacatcgctgcttagaacttgctcgccatttcctcatgccactttcccttcaaaaaggg 17
Query: 61 caatttggtt 70
|||| |||||
Sbjct: 16 caatctggtt 7
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 24,697
Number of Sequences: 369679
Number of extensions: 24697
Number of successful extensions: 6436
Number of sequences better than 0.5: 4
Number of HSP's better than 0.5 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 6426
Number of HSP's gapped (non-prelim): 9
length of query: 335
length of database: 203,408,664
effective HSP length: 18
effective length of query: 317
effective length of database: 196,754,442
effective search space: 62371158114
effective search space used: 62371158114
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)