BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 5735869.2.1
         (539 letters)

Database: Populus_nucl_with_EST.fasta 
           369,679 sequences; 203,408,664 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CV245012.1|CV245012  WS0256.B21_E01 PT-MB-N-A-15 Populus ...    38   0.33 
gb|DN488381.1|DN488381  R055F10.3pR Populus root cDNA librar...    38   0.33 

>gb|CV245012.1|CV245012 WS0256.B21_E01 PT-MB-N-A-15 Populus trichocarpa cDNA clone
          WS0256_E01 3', mRNA sequence
          Length = 976

 Score = 38.2 bits (19), Expect = 0.33
 Identities = 19/19 (100%)
 Strand = Plus / Plus

                             
Query: 53 ttgttccacagaagaatat 71
          |||||||||||||||||||
Sbjct: 40 ttgttccacagaagaatat 58



>gb|DN488381.1|DN488381 R055F10.3pR Populus root cDNA library Populus tremula x Populus
          tremuloides cDNA clone R055F10 3', mRNA sequence
          Length = 578

 Score = 38.2 bits (19), Expect = 0.33
 Identities = 19/19 (100%)
 Strand = Plus / Plus

                             
Query: 53 ttgttccacagaagaatat 71
          |||||||||||||||||||
Sbjct: 13 ttgttccacagaagaatat 31



  Database: Populus_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:49 PM
  Number of letters in database: 203,408,664
  Number of sequences in database:  369,679
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 78,000
Number of Sequences: 369679
Number of extensions: 78000
Number of successful extensions: 19258
Number of sequences better than  0.5: 2
Number of HSP's better than  0.5 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 19256
Number of HSP's gapped (non-prelim): 2
length of query: 539
length of database: 203,408,664
effective HSP length: 19
effective length of query: 520
effective length of database: 196,384,763
effective search space: 102120076760
effective search space used: 102120076760
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)