BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF25f04.yg.3.3
(1599 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR169326.1|DR169326 RTPHOS1_31_D02.g1_A029 Roots minus p... 98 1e-018
gb|DR159952.1|DR159952 RTFE1_2_E09.g1_A029 Roots minus iron... 94 2e-017
gb|BX249137.1|BX249137 BX249137 Pinus pinaster differenciat... 90 3e-016
gb|CO167537.1|CO167537 FLD1_69_C02.g1_A029 Root flooded Pin... 90 3e-016
gb|CO368479.1|CO368479 RTK1_40_H02.g1_A029 Roots minus pota... 90 3e-016
gb|DR078083.1|DR078083 RTFEPL1_1_G05.g1_A029 Roots plus add... 90 3e-016
gb|DR692511.1|DR692511 EST1082599 Normalized pine embryo li... 90 3e-016
gb|DT637158.1|DT637158 EST1152089 Normalized pine embryo li... 90 3e-016
gb|CO167456.1|CO167456 FLD1_69_C02.b1_A029 Root flooded Pin... 82 8e-014
gb|CO368392.1|CO368392 RTK1_40_H02.b1_A029 Roots minus pota... 50 3e-004
gb|DR078001.1|DR078001 RTFEPL1_1_G05.b1_A029 Roots plus add... 50 3e-004
>gb|DR169326.1|DR169326 RTPHOS1_31_D02.g1_A029 Roots minus phosphorous Pinus taeda cDNA clone
RTPHOS1_31_D02_A029 5', mRNA sequence
Length = 661
Score = 97.6 bits (49), Expect = 1e-018
Identities = 127/153 (83%)
Strand = Plus / Plus
Query: 1008 tgatcttccgagcttcaaaaaccatattagagatttcctggtgcagtcgaaggagttctc 1067
||||||| | || ||||| ||||| ||| |||| ||||| || || || || ||||||||
Sbjct: 492 tgatctttctagtttcaagaaccacattcgagacttccttgtccaatctaaagagttctc 551
Query: 1068 agctcaggataacaaggatctatacgcggaagaggctgctgcccaaagagaaagggagcg 1127
|||||||||||||| ||||| ||||| ||||| || ||||| ||||||||||| || ||
Sbjct: 552 tgctcaggataacaaagatctctacgcagaagaagcagctgcgcaaagagaaagagaacg 611
Query: 1128 ccaacgaatgcttgccattccggggctgattgc 1160
|||| |||||||| |||||| ||||| |||||
Sbjct: 612 ccaaagaatgcttagcattcctgggcttattgc 644
Score = 77.8 bits (39), Expect = 1e-012
Identities = 72/83 (86%)
Strand = Plus / Plus
Query: 907 tttgttcgagattacaccataaaactcttaggaacatcatttcctaacatgactgtagct 966
||||||||||| || ||||| || ||||||||||||||||||||||||||||| || ||
Sbjct: 391 tttgttcgagagtataccatcaagctcttaggaacatcatttcctaacatgacagtcact 450
Query: 967 gaggtgaccaagtttgtggatgg 989
||||| || | |||||||||||
Sbjct: 451 gaggttacacaatttgtggatgg 473
Score = 48.1 bits (24), Expect = 0.001
Identities = 66/80 (82%)
Strand = Plus / Plus
Query: 583 tcaagtcagcaattgaagcttgtgatcgattcaattaattgggcgttcagacatacagag 642
|||||||||||| |||||||||| || ||||| || | ||||| || | ||||| ||
Sbjct: 58 tcaagtcagcaactgaagcttgtcatggattccataatatgggcctttcgtcatactgaa 117
Query: 643 agaaatattgcagagactgg 662
||||||||||| ||||||||
Sbjct: 118 agaaatattgctgagactgg 137
>gb|DR159952.1|DR159952 RTFE1_2_E09.g1_A029 Roots minus iron Pinus taeda cDNA clone
RTFE1_2_E09_A029 5', mRNA sequence
Length = 801
Score = 93.7 bits (47), Expect = 2e-017
Identities = 182/227 (80%)
Strand = Plus / Plus
Query: 292 ccacatgtaggaaagcagtttgtaccaccaatgatggatcctgttcttggtgattatgct 351
|||||| |||| ||||| ||||||| ||| ||| |||||||| | |||||||| |||||
Sbjct: 37 ccacatatagggaagcaatttgtacaacctatgttggatcctatacttggtgactatgcg 96
Query: 352 agaaatgtacccgatgcaagggagtctgaagttctgtccttgtttgcaacaatcattaac 411
| ||||| || ||||| || |||||||||||||| || || |||||||| || || |||
Sbjct: 97 cggaatgtgcctgatgctagagagtctgaagttctatcattatttgcaactataataaac 156
Query: 412 aagtacaaaggggaaatgcttgaggatgtgcctcgtatatttgaggctgttttccagtgc 471
||||| |||| | ||| | |||||||| || ||||| ||||| |||||||| |||
Sbjct: 157 aagtataaagttgttatgatggaggatgtaccacgtatttttgaagctgtttttgcttgc 216
Query: 472 acccttgagatgattactaagaactttgaagattatcctgagcaccg 518
|| | |||||||||||||| || ||||| |||||||| || |||||
Sbjct: 217 acattagagatgattactaaaaattttgaggattatccggaacaccg 263
Score = 77.8 bits (39), Expect = 1e-012
Identities = 72/83 (86%)
Strand = Plus / Plus
Query: 907 tttgttcgagattacaccataaaactcttaggaacatcatttcctaacatgactgtagct 966
||||||||||| || ||||| || ||||||||||||||||||||||||||||| || ||
Sbjct: 661 tttgttcgagagtataccatcaagctcttaggaacatcatttcctaacatgacagtcact 720
Query: 967 gaggtgaccaagtttgtggatgg 989
||||| || | |||||||||||
Sbjct: 721 gaggttacacaatttgtggatgg 743
Score = 48.1 bits (24), Expect = 0.001
Identities = 66/80 (82%)
Strand = Plus / Plus
Query: 583 tcaagtcagcaattgaagcttgtgatcgattcaattaattgggcgttcagacatacagag 642
|||||||||||| |||||||||| || ||||| || | ||||| || | ||||| ||
Sbjct: 328 tcaagtcagcaactgaagcttgtcatggattccataatatgggcctttcgtcatactgaa 387
Query: 643 agaaatattgcagagactgg 662
||||||||||| ||||||||
Sbjct: 388 agaaatattgctgagactgg 407
>gb|BX249137.1|BX249137 BX249137 Pinus pinaster differenciating xylem adult Pinus pinaster
cDNA clone PP020D09, mRNA sequence
Length = 715
Score = 89.7 bits (45), Expect = 3e-016
Identities = 126/153 (82%)
Strand = Plus / Plus
Query: 1008 tgatcttccgagcttcaaaaaccatattagagatttcctggtgcagtcgaaggagttctc 1067
||||||| | || ||||| ||||| ||| |||| ||||| || || || || ||||||||
Sbjct: 337 tgatctttccagtttcaagaaccacattcgagacttccttgtccaatctaaagagttctc 396
Query: 1068 agctcaggataacaaggatctatacgcggaagaggctgctgcccaaagagaaagggagcg 1127
|||||||||||||| ||||| || || ||||| || ||||| ||||||||||| || ||
Sbjct: 397 tgctcaggataacaaagatctctatgcagaagaagcagctgcgcaaagagaaagagaacg 456
Query: 1128 ccaacgaatgcttgccattccggggctgattgc 1160
|||| |||||||| |||||| ||||| |||||
Sbjct: 457 ccaaagaatgcttagcattcctgggcttattgc 489
Score = 69.9 bits (35), Expect = 3e-010
Identities = 71/83 (85%)
Strand = Plus / Plus
Query: 907 tttgttcgagattacaccataaaactcttaggaacatcatttcctaacatgactgtagct 966
||||||||||| || ||||| || ||| ||||||||||||||||||||||||| || ||
Sbjct: 236 tttgttcgagagtataccatcaagctcctaggaacatcatttcctaacatgacagtcact 295
Query: 967 gaggtgaccaagtttgtggatgg 989
||||| || | |||||||||||
Sbjct: 296 gaggttacacaatttgtggatgg 318
>gb|CO167537.1|CO167537 FLD1_69_C02.g1_A029 Root flooded Pinus taeda cDNA clone
FLD1_69_C02_A029 5', mRNA sequence
Length = 709
Score = 89.7 bits (45), Expect = 3e-016
Identities = 126/153 (82%)
Strand = Plus / Plus
Query: 1008 tgatcttccgagcttcaaaaaccatattagagatttcctggtgcagtcgaaggagttctc 1067
||||||| | || ||||| ||||| ||| |||| ||||| || || || || ||||||||
Sbjct: 501 tgatctttctagtttcaagaaccacattcgagacttccttgtccaatctaaagagttctc 560
Query: 1068 agctcaggataacaaggatctatacgcggaagaggctgctgcccaaagagaaagggagcg 1127
|||||||||||||| ||||| || || ||||| || ||||| ||||||||||| || ||
Sbjct: 561 tgctcaggataacaaagatctctatgcagaagaagcagctgcgcaaagagaaagagaacg 620
Query: 1128 ccaacgaatgcttgccattccggggctgattgc 1160
|||| |||||||| |||||| ||||| |||||
Sbjct: 621 ccaaagaatgcttagcattcctgggcttattgc 653
Score = 77.8 bits (39), Expect = 1e-012
Identities = 72/83 (86%)
Strand = Plus / Plus
Query: 907 tttgttcgagattacaccataaaactcttaggaacatcatttcctaacatgactgtagct 966
||||||||||| || ||||| || ||||||||||||||||||||||||||||| || ||
Sbjct: 400 tttgttcgagagtataccatcaagctcttaggaacatcatttcctaacatgacagtcact 459
Query: 967 gaggtgaccaagtttgtggatgg 989
||||| || | |||||||||||
Sbjct: 460 gaggttacacaatttgtggatgg 482
Score = 40.1 bits (20), Expect = 0.27
Identities = 65/80 (81%)
Strand = Plus / Plus
Query: 583 tcaagtcagcaattgaagcttgtgatcgattcaattaattgggcgttcagacatacagag 642
||||||||||| |||||||||| || ||||| || | ||||| || | ||||| ||
Sbjct: 67 tcaagtcagcagctgaagcttgtcatggattccataatatgggcctttcgccatactgaa 126
Query: 643 agaaatattgcagagactgg 662
||||||||||| ||||||||
Sbjct: 127 agaaatattgctgagactgg 146
>gb|CO368479.1|CO368479 RTK1_40_H02.g1_A029 Roots minus potassium Pinus taeda cDNA clone
RTK1_40_H02_A029 5', mRNA sequence
Length = 783
Score = 89.7 bits (45), Expect = 3e-016
Identities = 126/153 (82%)
Strand = Plus / Plus
Query: 1008 tgatcttccgagcttcaaaaaccatattagagatttcctggtgcagtcgaaggagttctc 1067
||||||| | || ||||| ||||| ||| |||| ||||| || || || || ||||||||
Sbjct: 409 tgatctttctagtttcaagaaccacattcgagacttccttgtccaatctaaagagttctc 468
Query: 1068 agctcaggataacaaggatctatacgcggaagaggctgctgcccaaagagaaagggagcg 1127
|||||||||||||| ||||| || || ||||| || ||||| ||||||||||| || ||
Sbjct: 469 tgctcaggataacaaagatctctatgcagaagaagcagctgcgcaaagagaaagagaacg 528
Query: 1128 ccaacgaatgcttgccattccggggctgattgc 1160
|||| |||||||| |||||| ||||| |||||
Sbjct: 529 ccaaagaatgcttagcattcctgggcttattgc 561
Score = 77.8 bits (39), Expect = 1e-012
Identities = 72/83 (86%)
Strand = Plus / Plus
Query: 907 tttgttcgagattacaccataaaactcttaggaacatcatttcctaacatgactgtagct 966
||||||||||| || ||||| || ||||||||||||||||||||||||||||| || ||
Sbjct: 308 tttgttcgagagtataccatcaagctcttaggaacatcatttcctaacatgacagtcact 367
Query: 967 gaggtgaccaagtttgtggatgg 989
||||| || | |||||||||||
Sbjct: 368 gaggttacacaatttgtggatgg 390
>gb|DR078083.1|DR078083 RTFEPL1_1_G05.g1_A029 Roots plus added iron Pinus taeda cDNA clone
RTFEPL1_1_G05_A029 5', mRNA sequence
Length = 791
Score = 89.7 bits (45), Expect = 3e-016
Identities = 126/153 (82%)
Strand = Plus / Plus
Query: 1008 tgatcttccgagcttcaaaaaccatattagagatttcctggtgcagtcgaaggagttctc 1067
||||||| | || ||||| ||||| ||| |||| ||||| || || || || ||||||||
Sbjct: 426 tgatctttctagtttcaagaaccacattcgagacttccttgtccaatctaaagagttctc 485
Query: 1068 agctcaggataacaaggatctatacgcggaagaggctgctgcccaaagagaaagggagcg 1127
|||||||||||||| ||||| || || ||||| || ||||| ||||||||||| || ||
Sbjct: 486 tgctcaggataacaaagatctctatgcagaagaagcagctgcgcaaagagaaagagaacg 545
Query: 1128 ccaacgaatgcttgccattccggggctgattgc 1160
|||| |||||||| |||||| ||||| |||||
Sbjct: 546 ccaaagaatgcttagcattcctgggcttattgc 578
Score = 77.8 bits (39), Expect = 1e-012
Identities = 72/83 (86%)
Strand = Plus / Plus
Query: 907 tttgttcgagattacaccataaaactcttaggaacatcatttcctaacatgactgtagct 966
||||||||||| || ||||| || ||||||||||||||||||||||||||||| || ||
Sbjct: 325 tttgttcgagagtataccatcaagctcttaggaacatcatttcctaacatgacagtcact 384
Query: 967 gaggtgaccaagtttgtggatgg 989
||||| || | |||||||||||
Sbjct: 385 gaggttacacaatttgtggatgg 407
>gb|DR692511.1|DR692511 EST1082599 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWADN03 3' end, mRNA sequence
Length = 838
Score = 89.7 bits (45), Expect = 3e-016
Identities = 126/153 (82%)
Strand = Plus / Plus
Query: 1008 tgatcttccgagcttcaaaaaccatattagagatttcctggtgcagtcgaaggagttctc 1067
||||||| | || ||||| ||||| ||| |||| ||||| || || || || ||||||||
Sbjct: 295 tgatctttctagtttcaagaaccacattcgagacttccttgtccaatctaaagagttctc 354
Query: 1068 agctcaggataacaaggatctatacgcggaagaggctgctgcccaaagagaaagggagcg 1127
|||||||||||||| ||||| || || ||||| || ||||| ||||||||||| || ||
Sbjct: 355 tgctcaggataacaaagatctctatgcagaagaagcagctgcgcaaagagaaagagaacg 414
Query: 1128 ccaacgaatgcttgccattccggggctgattgc 1160
|||| |||||||| |||||| ||||| |||||
Sbjct: 415 ccaaagaatgcttagcattcctgggcttattgc 447
Score = 77.8 bits (39), Expect = 1e-012
Identities = 72/83 (86%)
Strand = Plus / Plus
Query: 907 tttgttcgagattacaccataaaactcttaggaacatcatttcctaacatgactgtagct 966
||||||||||| || ||||| || ||||||||||||||||||||||||||||| || ||
Sbjct: 194 tttgttcgagagtataccatcaagctcttaggaacatcatttcctaacatgacagtcact 253
Query: 967 gaggtgaccaagtttgtggatgg 989
||||| || | |||||||||||
Sbjct: 254 gaggttacacaatttgtggatgg 276
>gb|DT637158.1|DT637158 EST1152089 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMH036 3' end, mRNA sequence
Length = 828
Score = 89.7 bits (45), Expect = 3e-016
Identities = 126/153 (82%)
Strand = Plus / Plus
Query: 1008 tgatcttccgagcttcaaaaaccatattagagatttcctggtgcagtcgaaggagttctc 1067
||||||| | || ||||| ||||| ||| |||| ||||| || || || || ||||||||
Sbjct: 303 tgatctttctagtttcaagaaccacattcgagacttccttgtccaatctaaagagttctc 362
Query: 1068 agctcaggataacaaggatctatacgcggaagaggctgctgcccaaagagaaagggagcg 1127
|||||||||||||| ||||| || || ||||| || ||||| ||||||||||| || ||
Sbjct: 363 tgctcaggataacaaagatctctatgcagaagaagcagctgcgcaaagagaaagagaacg 422
Query: 1128 ccaacgaatgcttgccattccggggctgattgc 1160
|||| |||||||| |||||| ||||| |||||
Sbjct: 423 ccaaagaatgcttagcattcctgggcttattgc 455
Score = 77.8 bits (39), Expect = 1e-012
Identities = 72/83 (86%)
Strand = Plus / Plus
Query: 907 tttgttcgagattacaccataaaactcttaggaacatcatttcctaacatgactgtagct 966
||||||||||| || ||||| || ||||||||||||||||||||||||||||| || ||
Sbjct: 202 tttgttcgagagtataccatcaagctcttaggaacatcatttcctaacatgacagtcact 261
Query: 967 gaggtgaccaagtttgtggatgg 989
||||| || | |||||||||||
Sbjct: 262 gaggttacacaatttgtggatgg 284
>gb|CO167456.1|CO167456 FLD1_69_C02.b1_A029 Root flooded Pinus taeda cDNA clone
FLD1_69_C02_A029 3', mRNA sequence
Length = 830
Score = 81.8 bits (41), Expect = 8e-014
Identities = 86/101 (85%)
Strand = Plus / Plus
Query: 1060 gagttctcagctcaggataacaaggatctatacgcggaagaggctgctgcccaaagagaa 1119
|||||||| |||||||||||||| ||||| || || ||||| || ||||| |||||||||
Sbjct: 14 gagttctctgctcaggataacaaagatctctatgcagaagaagcagctgcgcaaagagaa 73
Query: 1120 agggagcgccaacgaatgcttgccattccggggctgattgc 1160
|| || |||||| |||||||| |||||| ||||| |||||
Sbjct: 74 agagaacgccaaagaatgcttagcattcctgggcttattgc 114
>gb|CO368392.1|CO368392 RTK1_40_H02.b1_A029 Roots minus potassium Pinus taeda cDNA clone
RTK1_40_H02_A029 3', mRNA sequence
Length = 792
Score = 50.1 bits (25), Expect = 3e-004
Identities = 55/65 (84%)
Strand = Plus / Plus
Query: 1096 gaagaggctgctgcccaaagagaaagggagcgccaacgaatgcttgccattccggggctg 1155
||||| || ||||| ||||||||||| || |||||| |||||||| |||||| |||||
Sbjct: 4 gaagaagcagctgcgcaaagagaaagagaacgccaaagaatgcttagcattcctgggctt 63
Query: 1156 attgc 1160
|||||
Sbjct: 64 attgc 68
>gb|DR078001.1|DR078001 RTFEPL1_1_G05.b1_A029 Roots plus added iron Pinus taeda cDNA clone
RTFEPL1_1_G05_A029 3', mRNA sequence
Length = 785
Score = 50.1 bits (25), Expect = 3e-004
Identities = 55/65 (84%)
Strand = Plus / Plus
Query: 1096 gaagaggctgctgcccaaagagaaagggagcgccaacgaatgcttgccattccggggctg 1155
||||| || ||||| ||||||||||| || |||||| |||||||| |||||| |||||
Sbjct: 6 gaagaagcagctgcgcaaagagaaagagaacgccaaagaatgcttagcattcctgggctt 65
Query: 1156 attgc 1160
|||||
Sbjct: 66 attgc 70
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 193,842
Number of Sequences: 355925
Number of extensions: 193842
Number of successful extensions: 50277
Number of sequences better than 0.5: 11
Number of HSP's better than 0.5 without gapping: 11
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 50225
Number of HSP's gapped (non-prelim): 48
length of query: 1599
length of database: 217,277,237
effective HSP length: 20
effective length of query: 1579
effective length of database: 210,158,737
effective search space: 331840645723
effective search space used: 331840645723
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)