BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 3345642.2.1
         (600 letters)

Database: MedicagoSativa_nucl_with_EST.fasta 
           7669 sequences; 3,745,706 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AF000976.1|MSAF000976  Medicago sativa 7-O-methyltransfer...    34   0.11 

>gb|AF000976.1|MSAF000976 Medicago sativa 7-O-methyltransferase (7-IOMT(9)) mRNA, complete
           cds
          Length = 1227

 Score = 34.2 bits (17), Expect = 0.11
 Identities = 20/21 (95%)
 Strand = Plus / Plus

                                
Query: 120 atgggcttttggaatgaacat 140
           ||||||| |||||||||||||
Sbjct: 125 atgggctgttggaatgaacat 145



  Database: MedicagoSativa_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:23 PM
  Number of letters in database: 3,745,706
  Number of sequences in database:  7669
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 1292
Number of Sequences: 7669
Number of extensions: 1292
Number of successful extensions: 324
Number of sequences better than  0.5: 1
Number of HSP's better than  0.5 without gapping: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 323
Number of HSP's gapped (non-prelim): 1
length of query: 600
length of database: 3,745,706
effective HSP length: 16
effective length of query: 584
effective length of database: 3,623,002
effective search space: 2115833168
effective search space used: 2115833168
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 16 (32.2 bits)