BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2619548.2.1
(691 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514103.1|CO514103 s13dSG75C1200086_156868 Glandular tr... 149 3e-036
gb|AW698393.1|AW698393 G240 glandular-haired subtracted cDN... 115 4e-026
gb|DQ122831.1| Medicago sativa clone C3 ribosomal protein L... 109 3e-024
gb|CO513652.1|CO513652 s13dSG17C0900066_140086 Glandular tr... 100 3e-021
gb|CO513365.1|CO513365 s13dSG38B0700057_129894 Glandular tr... 94 2e-019
gb|CO515624.1|CO515624 s13dSG47G0800068_418307 Glandular tr... 94 2e-019
gb|AF084204.1|AF084204 Medicago sativa clone MS62 unknown mRNA 58 9e-009
gb|CO516836.1|CO516836 s13dSG98D0200014_447000 Glandular tr... 32 0.49
>gb|CO514103.1|CO514103 s13dSG75C1200086_156868 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 505
Score = 149 bits (75), Expect = 3e-036
Identities = 255/315 (80%)
Strand = Plus / Plus
Query: 149 aagccaagctggcgcaggcccaagggtatcgactcccgcgtcaggcgtaagttcaaggga 208
|||| ||||||||| ||||| |||||||| || ||||| || |||||||||||||| |||
Sbjct: 137 aagcaaagctggcgtaggccaaagggtattgattcccgtgttaggcgtaagttcaaagga 196
Query: 209 tgcaccttgatgcccaacatcggatatggctccgacaagaagaccaggcactaccttccc 268
|| |||||||| ||||| ||||||||||| |||||||||||| | ||||| || ||
Sbjct: 197 gttactttgatgccaaacattggatatggctcagacaagaagacccgccactatctgcct 256
Query: 269 aacaagttcaaaaagtttgttgttcacaatgtctccgagctggagctgctgctgatgcac 328
|| ||||| ||||||||||| ||||||||| || |||| || || ||||||||
Sbjct: 257 aatggtttcaagaagtttgttgtgcacaatgtccaggatttggaacttttgatgatgcac 316
Query: 329 aacaggacttactgcgctgagatcgcccacaacgtgtccacccgcaagcgcaaggagatt 388
|||||||| ||||| ||||||||||| ||||| ||||| ||| | || | ||||||||
Sbjct: 317 aacaggacatactgtgctgagatcgcacacaatgtgtcaaccaggaaaagaaaggagatc 376
Query: 389 gtggagcgtgctgcgcaactggacatcgtggtcaccaacaagctcgccaggctccgcagc 448
|| || ||||| || ||| |||| | || || |||||||||||||||||| | |||||
Sbjct: 377 gttgaacgtgcagctcaattggatgttgttgttaccaacaagctcgccaggttgcgcagt 436
Query: 449 caagaggacgagtga 463
||||| |||||||||
Sbjct: 437 caagaagacgagtga 451
>gb|AW698393.1|AW698393 G240 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 405
Score = 115 bits (58), Expect = 4e-026
Identities = 178/218 (81%)
Strand = Plus / Plus
Query: 149 aagccaagctggcgcaggcccaagggtatcgactcccgcgtcaggcgtaagttcaaggga 208
|||| ||||||||| ||||| |||||||| || |||| || |||||||||||||| |||
Sbjct: 133 aagcaaagctggcgtaggccaaagggtattgattcccatgttaggcgtaagttcaaagga 192
Query: 209 tgcaccttgatgcccaacatcggatatggctccgacaagaagaccaggcactaccttccc 268
|| |||||||| ||||| ||||||||||| |||||||||||| | ||||| || ||
Sbjct: 193 gttactttgatgccaaacattggatatggctcagacaagaagacccgccactatctgcca 252
Query: 269 aacaagttcaaaaagtttgttgttcacaatgtctccgagctggagctgctgctgatgcac 328
|| ||||| ||||||||||| ||||||||| || |||| || || ||||||||
Sbjct: 253 aatggtttcaagaagtttgttgtgcacaatgtccaggatttggaacttttgatgatgcac 312
Query: 329 aacaggacttactgcgctgagatcgcccacaacgtgtc 366
|||||||| ||||| ||||||||||| ||||| |||||
Sbjct: 313 aacaggacatactgtgctgagatcgcacacaatgtgtc 350
>gb|DQ122831.1| Medicago sativa clone C3 ribosomal protein L32 mRNA, complete cds
Length = 558
Score = 109 bits (55), Expect = 3e-024
Identities = 250/315 (79%)
Strand = Plus / Plus
Query: 149 aagccaagctggcgcaggcccaagggtatcgactcccgcgtcaggcgtaagttcaaggga 208
|||| ||||||||||||||| |||||||| || || || || ||| | || ||||| |||
Sbjct: 148 aagcaaagctggcgcaggccaaagggtattgattcacgagttaggagaaaattcaaagga 207
Query: 209 tgcaccttgatgcccaacatcggatatggctccgacaagaagaccaggcactaccttccc 268
|| |||||||| ||||| ||||| ||||| |||||||||||| | ||||| | ||
Sbjct: 208 gttactttgatgcctaacattggatacggctcagacaagaagacccgccactatttgcct 267
Query: 269 aacaagttcaaaaagtttgttgttcacaatgtctccgagctggagctgctgctgatgcac 328
|| ||||| ||||| || || ||||||||| || |||||||| || ||||||||
Sbjct: 268 aatggattcaagaagttcgtcgtgcacaatgtccaggacctggagcttttgatgatgcac 327
Query: 329 aacaggacttactgcgctgagatcgcccacaacgtgtccacccgcaagcgcaaggagatt 388
|||||||| ||||| |||||||| || ||||| ||||| ||||| || | ||||||||
Sbjct: 328 aacaggacatactgtgctgagattgcacacaatgtgtcaacccgaaaaagaaaggagatc 387
Query: 389 gtggagcgtgctgcgcaactggacatcgtggtcaccaacaagctcgccaggctccgcagc 448
|| || ||||| || ||| |||| | || |||||||||||||| |||||| | |||||
Sbjct: 388 gttgaacgtgcagcacaattggatgttgttgtcaccaacaagctggccaggttgcgcagt 447
Query: 449 caagaggacgagtga 463
||||| |||||||||
Sbjct: 448 caagaagacgagtga 462
>gb|CO513652.1|CO513652 s13dSG17C0900066_140086 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 351
Score = 99.6 bits (50), Expect = 3e-021
Identities = 167/206 (81%)
Strand = Plus / Plus
Query: 149 aagccaagctggcgcaggcccaagggtatcgactcccgcgtcaggcgtaagttcaaggga 208
|||| ||||||||| ||||| |||||||| || ||||| || |||||||||||||| |||
Sbjct: 142 aagcaaagctggcgtaggccaaagggtattgattcccgtgttaggcgtaagttcaaagga 201
Query: 209 tgcaccttgatgcccaacatcggatatggctccgacaagaagaccaggcactaccttccc 268
|| |||||||| ||||| ||||||||||| |||||||||||| | || || || ||
Sbjct: 202 gttacgttgatgccaaacattggatatggctcagacaagaagacccgccattatctgcct 261
Query: 269 aacaagttcaaaaagtttgttgttcacaatgtctccgagctggagctgctgctgatgcac 328
|| ||||| ||||||||||| ||||||||| || |||| || || |||||||
Sbjct: 262 aatggtttcaagaagtttgttgtgcacaatgtccaggatttggaacttttgatgatgcat 321
Query: 329 aacaggacttactgcgctgagatcgc 354
|||||||| ||||| |||||||||||
Sbjct: 322 aacaggacatactgtgctgagatcgc 347
>gb|CO513365.1|CO513365 s13dSG38B0700057_129894 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 489
Score = 93.7 bits (47), Expect = 2e-019
Identities = 248/315 (78%)
Strand = Plus / Plus
Query: 149 aagccaagctggcgcaggcccaagggtatcgactcccgcgtcaggcgtaagttcaaggga 208
|||| ||||||||||||||| |||||||| || || || || ||| | || || || |||
Sbjct: 142 aagcaaagctggcgcaggccaaagggtattgattcacgagttaggagaaaatttaaagga 201
Query: 209 tgcaccttgatgcccaacatcggatatggctccgacaagaagaccaggcactaccttccc 268
|| |||||||| ||||| ||||| ||||| |||||||||||| | ||||| | ||
Sbjct: 202 gttactttgatgcctaacattggatacggctcagacaagaagacccgccactatttgcct 261
Query: 269 aacaagttcaaaaagtttgttgttcacaatgtctccgagctggagctgctgctgatgcac 328
|| ||||| |||||||| || ||||||||| || ||||| || || ||||||||
Sbjct: 262 aatggattcaagaagtttgtcgtgcacaatgtccaggacctggaacttttgatgatgcac 321
Query: 329 aacaggacttactgcgctgagatcgcccacaacgtgtccacccgcaagcgcaaggagatt 388
|||||||| ||||| |||||||| || ||||| ||||| ||| | || | ||||||||
Sbjct: 322 aacaggacatactgtgctgagattgcacacaatgtgtcaaccagaaaaagaaaggagatc 381
Query: 389 gtggagcgtgctgcgcaactggacatcgtggtcaccaacaagctcgccaggctccgcagc 448
|| || ||||| || ||| |||| | || |||||||||||||| |||||| | |||||
Sbjct: 382 gttgaacgtgcggcacaattggatgttgttgtcaccaacaagctggccaggttgcgcagt 441
Query: 449 caagaggacgagtga 463
||||| |||||||||
Sbjct: 442 caagaagacgagtga 456
>gb|CO515624.1|CO515624 s13dSG47G0800068_418307 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 349
Score = 93.7 bits (47), Expect = 2e-019
Identities = 149/183 (81%)
Strand = Plus / Plus
Query: 281 aagtttgttgttcacaatgtctccgagctggagctgctgctgatgcacaacaggacttac 340
||||||||||| ||||||||| || |||| || || |||||||||||||||| |||
Sbjct: 8 aagtttgttgtgcacaatgtccaggatttggaacttttgatgatgcacaacaggacatac 67
Query: 341 tgcgctgagatcgcccacaacgtgtccacccgcaagcgcaaggagattgtggagcgtgct 400
|| ||||||||||| ||||| ||||| ||| | || | |||||||| || || |||||
Sbjct: 68 tgtgctgagatcgcacacaatgtgtcaaccaggaaaagaaaggagatcgttgaacgtgca 127
Query: 401 gcgcaactggacatcgtggtcaccaacaagctcgccaggctccgcagccaagaggacgag 460
|| ||| |||| | || ||||||||||||||||||||| | ||||| ||||| ||||||
Sbjct: 128 gctcaattggatgttgttgtcaccaacaagctcgccaggttgcgcagtcaagaagacgag 187
Query: 461 tga 463
|||
Sbjct: 188 tga 190
>gb|AF084204.1|AF084204 Medicago sativa clone MS62 unknown mRNA
Length = 295
Score = 58.0 bits (29), Expect = 9e-009
Identities = 41/45 (91%)
Strand = Plus / Plus
Query: 419 gtcaccaacaagctcgccaggctccgcagccaagaggacgagtga 463
||||||||||||||||||||| | ||||| ||||| |||||||||
Sbjct: 66 gtcaccaacaagctcgccaggttgcgcagtcaagaagacgagtga 110
>gb|CO516836.1|CO516836 s13dSG98D0200014_447000 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 391
Score = 32.2 bits (16), Expect = 0.49
Identities = 16/16 (100%)
Strand = Plus / Minus
Query: 63 tggcggtgccgctgct 78
||||||||||||||||
Sbjct: 246 tggcggtgccgctgct 231
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 1266
Number of Sequences: 7669
Number of extensions: 1266
Number of successful extensions: 338
Number of sequences better than 0.5: 8
Number of HSP's better than 0.5 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 315
Number of HSP's gapped (non-prelim): 17
length of query: 691
length of database: 3,745,706
effective HSP length: 16
effective length of query: 675
effective length of database: 3,623,002
effective search space: 2445526350
effective search space used: 2445526350
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 16 (32.2 bits)