BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2542159.2.1
(1231 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AJ888506.1|AJ888506 AJ888506 Medicago sativa subsp. x va... 98 2e-020
gb|CO511712.1|CO511712 s13dSG01C0100006_103324 Glandular tr... 74 3e-013
gb|CO514165.1|CO514165 s13dSG70A1000069_156992 Glandular tr... 38 0.014
gb|CO515572.1|CO515572 s13dSG53H1100096_418203 Glandular tr... 38 0.014
gb|AF355597.1|AF355597 Medicago sativa MscN4 putative nodul... 36 0.057
gb|CO515322.1|CO515322 s13dSG48F0300031_417703 Glandular tr... 34 0.22
>gb|AJ888506.1|AJ888506 AJ888506 Medicago sativa subsp. x varia 4-day-old Medicago sativa
subsp. x varia cDNA, mRNA sequence
Length = 742
Score = 97.6 bits (49), Expect = 2e-020
Identities = 244/310 (78%)
Strand = Plus / Plus
Query: 156 tggtggactggttctatggggtgctggcatcgcttgggctgtggcagaaggaggctaaga 215
||||||| ||||| || ||||| || |||||||||| | |||||||| ||||||||||
Sbjct: 115 tggtggattggttttacggggttctancatcgcttggattatggcagaaagaggctaaga 174
Query: 216 tcctcttccttggcctcgacaacgccggcaagaccaccctcctccacatgctgaaggacg 275
|| | ||| | || ||||||||||||||||| |||||| | || || ||||| || |||
Sbjct: 175 tcttattcttaggtctcgacaacgccggcaaaaccaccttgcttcatatgctcaaagacn 234
Query: 276 agcggctcgtacagcaccagccgacgcagtaccccacgtcagaagagttgagcatcggca 335
|| | | || ||||| |||||||| ||||| || || |||||||||||||| || || |
Sbjct: 235 agagattagtccagcatcagccgactcagtatccgacatcagaagagttgagtattggga 294
Query: 336 ggatcaagttcaaggcgttcgaccttgggggccaccagatcgcccgccgcgtctggaagg 395
||||||||| || || || || | ||||| || ||||| || || |||||||| |
Sbjct: 295 agatcaagtttaaagcttttgatttggggggtcatcagattgctcgtanagtctggaaag 354
Query: 396 actactacgccaaggttgatgctgttgtgtacttggtggatgctgttgacaaggaacgtt 455
| ||||| ||| |||| ||||| || || |||||||| |||||| |||||||||| | |
Sbjct: 355 attactatgcccaggtagatgcagtggtctacttggttgatgcttatgacaaggaaagat 414
Query: 456 ttgccgagtc 465
|||| |||||
Sbjct: 415 ttgctgagtc 424
>gb|CO511712.1|CO511712 s13dSG01C0100006_103324 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 532
Score = 73.8 bits (37), Expect = 3e-013
Identities = 265/341 (77%)
Strand = Plus / Plus
Query: 386 gtctggaaggactactacgccaaggttgatgctgttgtgtacttggtggatgctgttgac 445
|||||||| || ||||| ||| |||| ||||| || || |||||||| |||||| ||||
Sbjct: 74 gtctggaaagattactatgcccaggtagatgcagtggtctacttggttgatgcttatgac 133
Query: 446 aaggaacgttttgccgagtcgaagaaggagctcgatgcgcttcttgcagatgactccctt 505
|||||| | ||||| ||||| || |||||| | ||||| || ||| | ||||| || |
Sbjct: 134 aaggaaagatttgctgagtcaaaaaaggagttggatgctctcctttctgatgaatcattg 193
Query: 506 gcaaacgttcctttcctcatactgggcaacaagattgacatcccatacgcggcttcagag 565
||||| || |||||||| | || || ||||| ||||| || ||||| || || |||||
Sbjct: 194 gcaaatgtccctttccttgtccttggaaacaaaattgatattccatatgctgcctcagaa 253
Query: 566 gaggagctgaggtactacctcggcctgagcaacttcacaaccgggaagggcaacgtgaac 625
|| || || | ||| | | ||| ||||||||||||| || || ||||| || || ||
Sbjct: 254 gacgaattgcgttaccatttgggcttgagcaacttcaccactggcaagggtaaggtaaat 313
Query: 626 ttggccgactccaatgtccggcccctggagatcttcatgtgcagtgtggtgcgcaagatg 685
||| | ||||| |||||||| ||| ||||| | |||||||||||| | ||| || |||
Sbjct: 314 ttgtcagactcaaatgtccgacccatggaggtattcatgtgcagtattgtgaagaaaatg 373
Query: 686 ggctatggcgaaggcttcaaatggatgtctcagtacatcaa 726
|| ||||| || || ||||||||| | ||||||||||||||
Sbjct: 374 ggttatggtgatggtttcaaatgggtttctcagtacatcaa 414
>gb|CO514165.1|CO514165 s13dSG70A1000069_156992 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 359
Score = 38.2 bits (19), Expect = 0.014
Identities = 73/91 (80%)
Strand = Plus / Plus
Query: 165 ggttctatggggtgctggcatcgcttgggctgtggcagaaggaggctaagatcctcttcc 224
|||||||||| | || ||||| ||||| || ||||| || || || ||||| |||||||
Sbjct: 169 ggttctatggcattcttgcatcccttggtctctggcaaaaagaagccaagattctcttcc 228
Query: 225 ttggcctcgacaacgccggcaagaccaccct 255
|||| || || || ||||| || ||||||||
Sbjct: 229 ttggtcttgataatgccggaaaaaccaccct 259
>gb|CO515572.1|CO515572 s13dSG53H1100096_418203 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 302
Score = 38.2 bits (19), Expect = 0.014
Identities = 22/23 (95%)
Strand = Plus / Plus
Query: 109 gaggaagaggaagaggaagagga 131
|||||||| ||||||||||||||
Sbjct: 92 gaggaagaagaagaggaagagga 114
>gb|AF355597.1|AF355597 Medicago sativa MscN4 putative nodule membrane protein mRNA,
complete cds
Length = 2367
Score = 36.2 bits (18), Expect = 0.057
Identities = 21/22 (95%)
Strand = Plus / Minus
Query: 107 aagaggaagaggaagaggaaga 128
||||||||||||||||| ||||
Sbjct: 222 aagaggaagaggaagagaaaga 201
Score = 34.2 bits (17), Expect = 0.22
Identities = 17/17 (100%)
Strand = Plus / Minus
Query: 113 aagaggaagaggaagag 129
|||||||||||||||||
Sbjct: 222 aagaggaagaggaagag 206
>gb|CO515322.1|CO515322 s13dSG48F0300031_417703 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 537
Score = 34.2 bits (17), Expect = 0.22
Identities = 17/17 (100%)
Strand = Plus / Minus
Query: 700 cttcaaatggatgtctc 716
|||||||||||||||||
Sbjct: 329 cttcaaatggatgtctc 313
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 2400
Number of Sequences: 7669
Number of extensions: 2400
Number of successful extensions: 755
Number of sequences better than 0.5: 6
Number of HSP's better than 0.5 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 741
Number of HSP's gapped (non-prelim): 12
length of query: 1231
length of database: 3,745,706
effective HSP length: 16
effective length of query: 1215
effective length of database: 3,623,002
effective search space: 4401947430
effective search space used: 4401947430
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)