BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2478065.2.2
(688 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514103.1|CO514103 s13dSG75C1200086_156868 Glandular tr... 143 2e-034
gb|AW698393.1|AW698393 G240 glandular-haired subtracted cDN... 135 5e-032
gb|CO513652.1|CO513652 s13dSG17C0900066_140086 Glandular tr... 119 3e-027
gb|DQ122831.1| Medicago sativa clone C3 ribosomal protein L... 115 4e-026
gb|CO513365.1|CO513365 s13dSG38B0700057_129894 Glandular tr... 107 1e-023
gb|CO515624.1|CO515624 s13dSG47G0800068_418307 Glandular tr... 78 9e-015
gb|AF020269.1|AF020269 Medicago sativa chloroplast malate d... 36 0.031
>gb|CO514103.1|CO514103 s13dSG75C1200086_156868 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 505
Score = 143 bits (72), Expect = 2e-034
Identities = 183/220 (83%)
Strand = Plus / Plus
Query: 212 tcaagccaagctggcgcaggcccaagggtatcgactcccgtgtcaggcggaagttcaagg 271
|||||| ||||||||| ||||| |||||||| || |||||||| ||||| |||||||| |
Sbjct: 135 tcaagcaaagctggcgtaggccaaagggtattgattcccgtgttaggcgtaagttcaaag 194
Query: 272 gatgcaccttgatgcctaacattggatatggctctgacaagaagaccaggcactatcttc 331
|| || |||||||| ||||||||||||||||| |||||||||||| | |||||||| |
Sbjct: 195 gagttactttgatgccaaacattggatatggctcagacaagaagacccgccactatctgc 254
Query: 332 ccaacaagtttaagaagtttgtcgtgcacaatgtttctgagctggagttgcttatgatgc 391
| || || ||||||||||| ||||||||||| || |||| | | |||||||
Sbjct: 255 ctaatggtttcaagaagtttgttgtgcacaatgtccaggatttggaacttttgatgatgc 314
Query: 392 acaacaggacatactgtgctgaaatcgcacacaacgtgtc 431
|||||||||||||||||||||| ||||||||||| |||||
Sbjct: 315 acaacaggacatactgtgctgagatcgcacacaatgtgtc 354
>gb|AW698393.1|AW698393 G240 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 405
Score = 135 bits (68), Expect = 5e-032
Identities = 182/220 (82%)
Strand = Plus / Plus
Query: 212 tcaagccaagctggcgcaggcccaagggtatcgactcccgtgtcaggcggaagttcaagg 271
|||||| ||||||||| ||||| |||||||| || |||| ||| ||||| |||||||| |
Sbjct: 131 tcaagcaaagctggcgtaggccaaagggtattgattcccatgttaggcgtaagttcaaag 190
Query: 272 gatgcaccttgatgcctaacattggatatggctctgacaagaagaccaggcactatcttc 331
|| || |||||||| ||||||||||||||||| |||||||||||| | |||||||| |
Sbjct: 191 gagttactttgatgccaaacattggatatggctcagacaagaagacccgccactatctgc 250
Query: 332 ccaacaagtttaagaagtttgtcgtgcacaatgtttctgagctggagttgcttatgatgc 391
| || || ||||||||||| ||||||||||| || |||| | | |||||||
Sbjct: 251 caaatggtttcaagaagtttgttgtgcacaatgtccaggatttggaacttttgatgatgc 310
Query: 392 acaacaggacatactgtgctgaaatcgcacacaacgtgtc 431
|||||||||||||||||||||| ||||||||||| |||||
Sbjct: 311 acaacaggacatactgtgctgagatcgcacacaatgtgtc 350
>gb|CO513652.1|CO513652 s13dSG17C0900066_140086 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 351
Score = 119 bits (60), Expect = 3e-027
Identities = 174/212 (82%)
Strand = Plus / Plus
Query: 212 tcaagccaagctggcgcaggcccaagggtatcgactcccgtgtcaggcggaagttcaagg 271
|||||| ||||||||| ||||| |||||||| || |||||||| ||||| |||||||| |
Sbjct: 140 tcaagcaaagctggcgtaggccaaagggtattgattcccgtgttaggcgtaagttcaaag 199
Query: 272 gatgcaccttgatgcctaacattggatatggctctgacaagaagaccaggcactatcttc 331
|| || |||||||| ||||||||||||||||| |||||||||||| | || ||||| |
Sbjct: 200 gagttacgttgatgccaaacattggatatggctcagacaagaagacccgccattatctgc 259
Query: 332 ccaacaagtttaagaagtttgtcgtgcacaatgtttctgagctggagttgcttatgatgc 391
| || || ||||||||||| ||||||||||| || |||| | | |||||||
Sbjct: 260 ctaatggtttcaagaagtttgttgtgcacaatgtccaggatttggaacttttgatgatgc 319
Query: 392 acaacaggacatactgtgctgaaatcgcacac 423
| |||||||||||||||||||| |||||||||
Sbjct: 320 ataacaggacatactgtgctgagatcgcacac 351
>gb|DQ122831.1| Medicago sativa clone C3 ribosomal protein L32 mRNA, complete cds
Length = 558
Score = 115 bits (58), Expect = 4e-026
Identities = 178/218 (81%)
Strand = Plus / Plus
Query: 214 aagccaagctggcgcaggcccaagggtatcgactcccgtgtcaggcggaagttcaaggga 273
|||| ||||||||||||||| |||||||| || || || || ||| | || ||||| |||
Sbjct: 148 aagcaaagctggcgcaggccaaagggtattgattcacgagttaggagaaaattcaaagga 207
Query: 274 tgcaccttgatgcctaacattggatatggctctgacaagaagaccaggcactatcttccc 333
|| |||||||||||||||||||| ||||| |||||||||||| | |||||| | ||
Sbjct: 208 gttactttgatgcctaacattggatacggctcagacaagaagacccgccactatttgcct 267
Query: 334 aacaagtttaagaagtttgtcgtgcacaatgtttctgagctggagttgcttatgatgcac 393
|| || |||||||| |||||||||||||| || |||||| | | |||||||||
Sbjct: 268 aatggattcaagaagttcgtcgtgcacaatgtccaggacctggagcttttgatgatgcac 327
Query: 394 aacaggacatactgtgctgaaatcgcacacaacgtgtc 431
|||||||||||||||||||| || |||||||| |||||
Sbjct: 328 aacaggacatactgtgctgagattgcacacaatgtgtc 365
Score = 36.2 bits (18), Expect = 0.031
Identities = 24/26 (92%)
Strand = Plus / Plus
Query: 149 agaagatcgtgaagaagcgggtcaag 174
||||||| ||||||||||| ||||||
Sbjct: 83 agaagattgtgaagaagcgtgtcaag 108
>gb|CO513365.1|CO513365 s13dSG38B0700057_129894 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 489
Score = 107 bits (54), Expect = 1e-023
Identities = 177/218 (81%)
Strand = Plus / Plus
Query: 214 aagccaagctggcgcaggcccaagggtatcgactcccgtgtcaggcggaagttcaaggga 273
|||| ||||||||||||||| |||||||| || || || || ||| | || || || |||
Sbjct: 142 aagcaaagctggcgcaggccaaagggtattgattcacgagttaggagaaaatttaaagga 201
Query: 274 tgcaccttgatgcctaacattggatatggctctgacaagaagaccaggcactatcttccc 333
|| |||||||||||||||||||| ||||| |||||||||||| | |||||| | ||
Sbjct: 202 gttactttgatgcctaacattggatacggctcagacaagaagacccgccactatttgcct 261
Query: 334 aacaagtttaagaagtttgtcgtgcacaatgtttctgagctggagttgcttatgatgcac 393
|| || ||||||||||||||||||||||| || ||||| | | |||||||||
Sbjct: 262 aatggattcaagaagtttgtcgtgcacaatgtccaggacctggaacttttgatgatgcac 321
Query: 394 aacaggacatactgtgctgaaatcgcacacaacgtgtc 431
|||||||||||||||||||| || |||||||| |||||
Sbjct: 322 aacaggacatactgtgctgagattgcacacaatgtgtc 359
Score = 36.2 bits (18), Expect = 0.031
Identities = 24/26 (92%)
Strand = Plus / Plus
Query: 149 agaagatcgtgaagaagcgggtcaag 174
||||||| ||||||||||| ||||||
Sbjct: 77 agaagattgtgaagaagcgtgtcaag 102
>gb|CO515624.1|CO515624 s13dSG47G0800068_418307 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 349
Score = 77.8 bits (39), Expect = 9e-015
Identities = 45/47 (95%)
Strand = Plus / Plus
Query: 385 atgatgcacaacaggacatactgtgctgaaatcgcacacaacgtgtc 431
||||||||||||||||||||||||||||| ||||||||||| |||||
Sbjct: 47 atgatgcacaacaggacatactgtgctgagatcgcacacaatgtgtc 93
>gb|AF020269.1|AF020269 Medicago sativa chloroplast malate dehydrogenase precursor (p1mdh)
mRNA, nuclear gene encoding chloroplast protein,
complete cds
Length = 2050
Score = 36.2 bits (18), Expect = 0.031
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 293 ttggatatggctctgaca 310
||||||||||||||||||
Sbjct: 139 ttggatatggctctgaca 156
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 1018
Number of Sequences: 7669
Number of extensions: 1018
Number of successful extensions: 300
Number of sequences better than 0.5: 7
Number of HSP's better than 0.5 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 278
Number of HSP's gapped (non-prelim): 19
length of query: 688
length of database: 3,745,706
effective HSP length: 16
effective length of query: 672
effective length of database: 3,623,002
effective search space: 2434657344
effective search space used: 2434657344
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 16 (32.2 bits)