BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1716236.2.2
(868 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514742.1|CO514742 s13dSG55F0200027_328144 Glandular tr... 206 2e-053
gb|CO514307.1|CO514307 s13dSG76H0900076_157276 Glandular tr... 188 4e-048
gb|CO512082.1|CO512082 s13dSG18F0600047_108484 Glandular tr... 182 3e-046
gb|CO516470.1|CO516470 s13dSG28C0900068_446268 Glandular tr... 182 3e-046
gb|CO514801.1|CO514801 s13dSG59D0300030_328262 Glandular tr... 178 4e-045
gb|AW698741.1|AW698741 r315 non-glandular-haired subtracted... 147 2e-035
gb|CO515876.1|CO515876 s13dSG91B0900075_421291 Glandular tr... 101 8e-022
gb|CO512153.1|CO512153 s13dSG34E0900067_108626 Glandular tr... 98 1e-020
gb|CO511830.1|CO511830 s13dSG02E0400035_103560 Glandular tr... 90 3e-018
gb|AJ410088.1|AJ410088 AJ410088 Medicago sativa library (Ka... 70 3e-012
gb|CO513129.1|CO513129 s13dSG23C0100002_129422 Glandular tr... 70 3e-012
emb|X74947.1|MSANN M.sativa mRNA for annexin 40 0.003
gb|CO515347.1|CO515347 s13dSG49A0400033_417753 Glandular tr... 34 0.16
>gb|CO514742.1|CO514742 s13dSG55F0200027_328144 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 526
Score = 206 bits (104), Expect = 2e-053
Identities = 362/448 (80%)
Strand = Plus / Plus
Query: 112 catggcatccaaacgtatcctcaaggaattaaaggacttgcaaaaggaccctccaacatc 171
||||||||| ||||| ||||||||||| | ||||| ||||| ||||| || ||||||||
Sbjct: 73 catggcatcgaaacggatcctcaaggagctcaaggatttgcagaaggatccaccaacatc 132
Query: 172 atgcagtgcaggtcctgctggtgaggatatgttccactggcaggcaaccatcatgggtcc 231
||||||||||||||||| | |||||| ||||| || ||||| ||||| |||||||| ||
Sbjct: 133 atgcagtgcaggtcctgtggctgaggacatgtttcattggcaagcaacaatcatgggacc 192
Query: 232 tcctgatagtccatatgctggaggtgttttcttggtgaacattcatttcccgccagacta 291
| ||||||| || |||||||| ||||| || ||||| |||||||| || || || ||
Sbjct: 193 tgctgatagcccctatgctggtggtgtgtttttggtttcaattcattttcctccggatta 252
Query: 292 ccccttcaagcctccaaaggtatcttttaagacgaaggtcttccatccaaacatcaatag 351
|| |||||||| |||||||| | |||| ||| ||||| ||||| ||||||||||| ||
Sbjct: 253 tccattcaagccaccaaaggttgcatttaggactaaggtattccacccaaacatcaacag 312
Query: 352 taatggaagcatatgtcttgacattcttaaggagcagtggagccctgctttgacaatctc 411
|||||||| || ||||| |||||||||||||| || ||||||||||| |||| || ||
Sbjct: 313 caatggaagtatttgtctcgacattcttaaggaacaatggagccctgcactgaccatttc 372
Query: 412 taaggttttgctttctatctgctccctgcttactgatcccaacccagacgaccctcttgt 471
||||| | | || |||||||| || | || |||||||| || || || || |||||
Sbjct: 373 caaggtgcttttgtcaatctgctcattggtgacggatcccaatcccgatgatccgcttgt 432
Query: 472 ccctgagattgctcacatgtacaagacggatcggcctaagtacgaggcgacagcccgcag 531
||||| ||||| || ||||||||||| || || | ||||| || || || || |||||
Sbjct: 433 gcctgaaattgcacatatgtacaagactgacaggaccaagtatgaagccaccgcacgcag 492
Query: 532 ctggacccagaagtacgccatgggatga 559
|||||| |||||||| ||||||||||||
Sbjct: 493 ctggacacagaagtatgccatgggatga 520
>gb|CO514307.1|CO514307 s13dSG76H0900076_157276 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 490
Score = 188 bits (95), Expect = 4e-048
Identities = 314/387 (81%)
Strand = Plus / Plus
Query: 112 catggcatccaaacgtatcctcaaggaattaaaggacttgcaaaaggaccctccaacatc 171
||||||||| ||||| ||||||||||| | ||||| ||||| ||||| || ||||||||
Sbjct: 99 catggcatcgaaacggatcctcaaggagctcaaggatttgcagaaggatccaccaacatc 158
Query: 172 atgcagtgcaggtcctgctggtgaggatatgttccactggcaggcaaccatcatgggtcc 231
||||||||||||||||| | |||||| ||||| || ||||| ||||| |||||||| ||
Sbjct: 159 atgcagtgcaggtcctgtggctgaggacatgtttcattggcaagcaacaatcatgggacc 218
Query: 232 tcctgatagtccatatgctggaggtgttttcttggtgaacattcatttcccgccagacta 291
| ||||||| || |||||||| ||||| || ||||| |||||||| || || || ||
Sbjct: 219 tgctgatagcccctatgctggtggtgtgtttttggtttcaattcattttcctccggatta 278
Query: 292 ccccttcaagcctccaaaggtatcttttaagacgaaggtcttccatccaaacatcaatag 351
|| |||||||| |||||||| | |||| ||| ||||| ||||| ||||||||||| ||
Sbjct: 279 tccattcaagccaccaaaggttgcatttaggactaaggtattccacccaaacatcaacag 338
Query: 352 taatggaagcatatgtcttgacattcttaaggagcagtggagccctgctttgacaatctc 411
|||||||| || ||||| |||||||||||||| || ||||||||||| |||| || ||
Sbjct: 339 caatggaagtatttgtctcgacattcttaaggaacaatggagccctgcactgaccatttc 398
Query: 412 taaggttttgctttctatctgctccctgcttactgatcccaacccagacgaccctcttgt 471
||||| | | || |||||||| || | || |||||||| || || ||||| |||||
Sbjct: 399 caaggtgcttttgtcaatctgctcattgttgacggatcccaatcccgatgacccgcttgt 458
Query: 472 ccctgagattgctcacatgtacaagac 498
||||| ||||| || |||||||||||
Sbjct: 459 gcctgaaattgcacatatgtacaagac 485
>gb|CO512082.1|CO512082 s13dSG18F0600047_108484 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 587
Score = 182 bits (92), Expect = 3e-046
Identities = 239/288 (82%)
Strand = Plus / Plus
Query: 112 catggcatccaaacgtatcctcaaggaattaaaggacttgcaaaaggaccctccaacatc 171
||||||||| ||||| ||||||||||| | ||||| ||||| ||||| || ||||||||
Sbjct: 197 catggcatcgaaacggatcctcaaggagctcaaggatttgcagaaggatccaccaacatc 256
Query: 172 atgcagtgcaggtcctgctggtgaggatatgttccactggcaggcaaccatcatgggtcc 231
||||||||||||||||| | |||||| ||||| || ||||| ||||| |||||||| ||
Sbjct: 257 atgcagtgcaggtcctgtggctgaggacatgtttcattggcaagcaacaatcatgggacc 316
Query: 232 tcctgatagtccatatgctggaggtgttttcttggtgaacattcatttcccgccagacta 291
| ||||||| || |||||||| ||||| || ||||| |||||||| || || || ||
Sbjct: 317 tgctgatagcccctatgctggtggtgtgtttttggtttcaattcattttcctccggatta 376
Query: 292 ccccttcaagcctccaaaggtatcttttaagacgaaggtcttccatccaaacatcaatag 351
|| |||||||| |||||||| | |||| ||| ||||| ||||| ||||||||||| ||
Sbjct: 377 tccattcaagccaccaaaggttgcatttaggactaaggtattccacccaaacatcaacag 436
Query: 352 taatggaagcatatgtcttgacattcttaaggagcagtggagccctgc 399
|||||||| || ||||| |||||||||||||| || |||||||||||
Sbjct: 437 caatggaagtatttgtctcgacattcttaaggaacaatggagccctgc 484
>gb|CO516470.1|CO516470 s13dSG28C0900068_446268 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 418
Score = 182 bits (92), Expect = 3e-046
Identities = 239/288 (82%)
Strand = Plus / Plus
Query: 112 catggcatccaaacgtatcctcaaggaattaaaggacttgcaaaaggaccctccaacatc 171
||||||||| ||||| ||||||||||| | ||||| ||||| ||||| || ||||||||
Sbjct: 74 catggcatcgaaacggatcctcaaggagctcaaggatttgcagaaggatccaccaacatc 133
Query: 172 atgcagtgcaggtcctgctggtgaggatatgttccactggcaggcaaccatcatgggtcc 231
||||||||||||||||| | |||||| ||||| || ||||| ||||| |||||||| ||
Sbjct: 134 atgcagtgcaggtcctgtggctgaggacatgtttcattggcaagcaacaatcatgggacc 193
Query: 232 tcctgatagtccatatgctggaggtgttttcttggtgaacattcatttcccgccagacta 291
| ||||||| || |||||||| ||||| || ||||| |||||||| || || || ||
Sbjct: 194 tgctgatagcccctatgctggtggtgtgtttttggtttcaattcattttcctccggatta 253
Query: 292 ccccttcaagcctccaaaggtatcttttaagacgaaggtcttccatccaaacatcaatag 351
|| |||||||| |||||||| | |||| ||| ||||| ||||| ||||||||||| ||
Sbjct: 254 tccattcaagccaccaaaggttgcatttaggactaaggtattccacccaaacatcaacag 313
Query: 352 taatggaagcatatgtcttgacattcttaaggagcagtggagccctgc 399
|||||||| || ||||| |||||||||||||| || |||||||||||
Sbjct: 314 caatggaagtatttgtctcgacattcttaaggaacaatggagccctgc 361
>gb|CO514801.1|CO514801 s13dSG59D0300030_328262 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 586
Score = 178 bits (90), Expect = 4e-045
Identities = 213/254 (83%)
Strand = Plus / Plus
Query: 143 aaggacttgcaaaaggaccctccaacatcatgcagtgcaggtcctgctggtgaggatatg 202
||||| ||||| |||||||| ||||| ||||||||||||||||||| | |||||| |||
Sbjct: 115 aaggatttgcagaaggaccccccaacttcatgcagtgcaggtcctgtggctgaggacatg 174
Query: 203 ttccactggcaggcaaccatcatgggtcctcctgatagtccatatgctggaggtgttttc 262
||||||||||| || || |||||||| || ||||||| |||||| |||| ||||| |||
Sbjct: 175 ttccactggcaagccacaatcatgggaccggctgatagcccatatactggtggtgtgttc 234
Query: 263 ttggtgaacattcatttcccgccagactaccccttcaagcctccaaaggtatcttttaag 322
|||| |||||||| || || ||||| ||||||||||| ||||||| |||||| |
Sbjct: 235 ctggtttcaattcattttcctcctgactatcccttcaagccaccaaagggtgcttttagg 294
Query: 323 acgaaggtcttccatccaaacatcaatagtaatggaagcatatgtcttgacattcttaag 382
|| ||||| ||||| ||||||||||| |||||||| || |||||||| |||||||| |||
Sbjct: 295 accaaggtgttccacccaaacatcaacagtaatggcagtatatgtctcgacattctgaag 354
Query: 383 gagcagtggagccc 396
|| |||||||||||
Sbjct: 355 gaacagtggagccc 368
Score = 69.9 bits (35), Expect = 3e-012
Identities = 92/111 (82%)
Strand = Plus / Plus
Query: 449 cccaacccagacgaccctcttgtccctgagattgctcacatgtacaagacggatcggcct 508
|||||||| || || || ||||| ||||| ||||| |||||||||||||| || || |
Sbjct: 421 cccaaccccgatgatccacttgttcctgaaattgcacacatgtacaagaccgacagggcc 480
Query: 509 aagtacgaggcgacagcccgcagctggacccagaagtacgccatgggatga 559
|| || || || ||||| ||||||||||| |||||||| ||||||||||||
Sbjct: 481 aaatatgaagccacagctcgcagctggacacagaagtatgccatgggatga 531
>gb|AW698741.1|AW698741 r315 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 335
Score = 147 bits (74), Expect = 2e-035
Identities = 196/237 (82%)
Strand = Plus / Minus
Query: 163 tccaacatcatgcagtgcaggtcctgctggtgaggatatgttccactggcaggcaaccat 222
|||||||||||||||||||||||||| | |||||| ||||| || ||||| ||||| ||
Sbjct: 328 tccaacatcatgcagtgcaggtcctgtggctgaggacatgtttcattggcaagcaacaat 269
Query: 223 catgggtcctcctgatagtccatatgctggaggtgttttcttggtgaacattcatttccc 282
|||||| ||| ||||||| || |||||||| ||||| || ||||| |||||||| ||
Sbjct: 268 catgggacctgctgatagcccctatgctggtggtgtgtttttggtttcaattcattttcc 209
Query: 283 gccagactaccccttcaagcctccaaaggtatcttttaagacgaaggtcttccatccaaa 342
|| || || || |||||||| |||||||| | | || ||| ||||| ||||| |||||
Sbjct: 208 tccggattatccattcaagccaccaaaggttgcatntaggactaaggtattccacccaaa 149
Query: 343 catcaatagtaatggaagcatatgtcttgacattcttaaggagcagtggagccctgc 399
|||||| || |||||||| || ||||| |||||||||||||| || |||||||||||
Sbjct: 148 catcaacagcaatggaagtatttgtctcgacattcttaaggaacaatggagccctgc 92
>gb|CO515876.1|CO515876 s13dSG91B0900075_421291 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 518
Score = 101 bits (51), Expect = 8e-022
Identities = 303/387 (78%)
Strand = Plus / Plus
Query: 112 catggcatccaaacgtatcctcaaggaattaaaggacttgcaaaaggaccctccaacatc 171
|||||| ||||| || |||||||||||| | |||||| | || || || |||||||| ||
Sbjct: 92 catggcctccaagcggatcctcaaggaactcaaggaccttcagaaagatcctccaacctc 151
Query: 172 atgcagtgcaggtcctgctggtgaggatatgttccactggcaggcaaccatcatgggtcc 231
||||| || || |||| | || || ||||| || ||||| ||||| || ||||||||
Sbjct: 152 ttgcagcgctggccctgtagcagaagacatgtttcattggcaagcaactataatgggtcc 211
Query: 232 tcctgatagtccatatgctggaggtgttttcttggtgaacattcatttcccgccagacta 291
|| ||||| || ||||| ||||| ||||| | || | ||||||||| || || || ||
Sbjct: 212 ccccgatagcccttatgccggaggagtttttctagttaccattcattttcctcctgatta 271
Query: 292 ccccttcaagcctccaaaggtatcttttaagacgaaggtcttccatccaaacatcaatag 351
|| |||||||| || ||||| | |||| ||| || || ||||| ||||| ||||| ||
Sbjct: 272 tccattcaagccacccaaggttgcatttaggaccaaagttttccacccaaatatcaacag 331
Query: 352 taatggaagcatatgtcttgacattcttaaggagcagtggagccctgctttgacaatctc 411
||||| ||||| |||||||| || | ||||||||||||||||| ||||||||||| ||
Sbjct: 332 caatggtagcatttgtcttgatatcttgaaggagcagtggagccccgctttgacaatatc 391
Query: 412 taaggttttgctttctatctgctccctgcttactgatcccaacccagacgaccctcttgt 471
|||||| || | || || || |||||| | || ||||| |||||||| || || | ||
Sbjct: 392 taaggtgctgttatccatttgttccctgttgacggatccaaacccagatgatcccttagt 451
Query: 472 ccctgagattgctcacatgtacaagac 498
||||| || ||||| |||||||||||
Sbjct: 452 gcctgaaatagctcatatgtacaagac 478
>gb|CO512153.1|CO512153 s13dSG34E0900067_108626 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 582
Score = 97.6 bits (49), Expect = 1e-020
Identities = 286/365 (78%)
Strand = Plus / Plus
Query: 134 aaggaattaaaggacttgcaaaaggaccctccaacatcatgcagtgcaggtcctgctggt 193
||||| || ||||||||||| || |||||||| || |||||||| || |||| ||||
Sbjct: 106 aaggagttgaaggacttgcagaaagaccctcctgtgtcttgcagtgctggccctgttggt 165
Query: 194 gaggatatgttccactggcaggcaaccatcatgggtcctcctgatagtccatatgctgga 253
|| || |||| || ||||| || || |||||||| || | || ||||| ||||||||
Sbjct: 166 gaagacctgtttcattggcaagctactatcatgggaccggcagacagtccttatgctggt 225
Query: 254 ggtgttttcttggtgaacattcatttcccgccagactaccccttcaagcctccaaaggta 313
||||| ||| | |||| |||||| ||||| ||||| || || | ||||| ||||||||
Sbjct: 226 ggtgtattcctagtgaccattcacttcccaccagattatccatctaagccaccaaaggtt 285
Query: 314 tcttttaagacgaaggtcttccatccaaacatcaatagtaatggaagcatatgtcttgac 373
|| ||| || || || ||||| || |||||||| || ||||| || || |||||||||
Sbjct: 286 tcctttcgaacaaaagtgttccaccctaacatcaacagcaatgggagtatctgtcttgac 345
Query: 374 attcttaaggagcagtggagccctgctttgacaatctctaaggttttgctttctatctgc 433
||||| || ||||||||||| |||||| | || | || ||||| |||| || || |||
Sbjct: 346 attctgaaagagcagtggagtcctgctcttactgtttcgaaggtcctgctgtcaatttgc 405
Query: 434 tccctgcttactgatcccaacccagacgaccctcttgtccctgagattgctcacatgtac 493
|| ||||| || ||||| |||||||| || ||| | || ||||| ||||| |||||||||
Sbjct: 406 tctctgctgacagatccaaacccagatgatcctttggtgcctgatattgcccacatgtac 465
Query: 494 aagac 498
|||||
Sbjct: 466 aagac 470
>gb|CO511830.1|CO511830 s13dSG02E0400035_103560 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 519
Score = 89.7 bits (45), Expect = 3e-018
Identities = 287/365 (78%), Gaps = 2/365 (0%)
Strand = Plus / Plus
Query: 134 aaggaattaaaggacttgcaaaaggaccctccaacatcatgcagtgcaggtcctgctggt 193
|||||||| ||||||||||| ||||| ||||| | || |||||||| || |||| | |
Sbjct: 115 aaggaattgaaggacttgcagaaggatcctcctgcttcttgcagtgctggccctgtcgct 174
Query: 194 gaggatatgttccactggcaggcaaccatcatgggtcctcctgatagtccatatgctgga 253
|| ||||||||||| ||||| || || || ||||| || ||||| |||| ||| |||
Sbjct: 175 gatgatatgttccattggcaagctacaattatgggcccaggagatagcccatttgcggga 234
Query: 254 ggtgt-tttcttggtgaacattcatttcccgccagactaccccttcaagcctccaaaggt 312
||||| ||||||| || | ||||| ||||| || || || || ||||| || || |||||
Sbjct: 235 ggtgtgtttcttg-tgcaaattcacttccctccggattatcctttcaaaccacccaaggt 293
Query: 313 atcttttaagacgaaggtcttccatccaaacatcaatagtaatggaagcatatgtcttga 372
|| || || ||||| ||||| || || ||||| ||||| || ||||| || |||||
Sbjct: 294 ttcattccgcacaaaggttttccaccctaatatcaacagtaacggtagcatttgccttga 353
Query: 373 cattcttaaggagcagtggagccctgctttgacaatctctaaggttttgctttctatctg 432
||| || || ||||| ||||||||||| || ||||| || ||||| | || || || ||
Sbjct: 354 catcctgaaagagcaatggagccctgccttcacaatatccaaggtgctactgtccatttg 413
Query: 433 ctccctgcttactgatcccaacccagacgaccctcttgtccctgagattgctcacatgta 492
|| ||| | ||||||||||||||||| || ||||| |||||||| |||||||| |||||
Sbjct: 414 ttctctgttgactgatcccaacccagatgatcctctggtccctgaaattgctcatatgta 473
Query: 493 caaga 497
|||||
Sbjct: 474 caaga 478
>gb|AJ410088.1|AJ410088 AJ410088 Medicago sativa library (Kalo P) Medicago sativa cDNA
clone L482, mRNA sequence
Length = 320
Score = 69.9 bits (35), Expect = 3e-012
Identities = 50/55 (90%)
Strand = Plus / Plus
Query: 443 actgatcccaacccagacgaccctcttgtccctgagattgctcacatgtacaaga 497
||||||||||||||||| || ||||| |||||||| |||||||| ||||||||||
Sbjct: 2 actgatcccaacccagatgatcctctggtccctgaaattgctcatatgtacaaga 56
>gb|CO513129.1|CO513129 s13dSG23C0100002_129422 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 517
Score = 69.9 bits (35), Expect = 3e-012
Identities = 92/111 (82%)
Strand = Plus / Plus
Query: 449 cccaacccagacgaccctcttgtccctgagattgctcacatgtacaagacggatcggcct 508
|||||||| || || || ||||| ||||| | ||| |||||||||||||| || || |
Sbjct: 20 cccaaccccgatgatccacttgttcctgaaactgcacacatgtacaagaccgacagggcc 79
Query: 509 aagtacgaggcgacagcccgcagctggacccagaagtacgccatgggatga 559
||||| || || ||||| ||||||||||| |||||||| ||||||||||||
Sbjct: 80 aagtatgaagccacagctcgcagctggacacagaagtatgccatgggatga 130
>emb|X74947.1|MSANN M.sativa mRNA for annexin
Length = 1182
Score = 40.1 bits (20), Expect = 0.003
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 728 cttgctgtggtaactatgct 747
||||||||||||||||||||
Sbjct: 1039 cttgctgtggtaactatgct 1058
>gb|CO515347.1|CO515347 s13dSG49A0400033_417753 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 495
Score = 34.2 bits (17), Expect = 0.16
Identities = 17/17 (100%)
Strand = Plus / Plus
Query: 87 tccagatccagatccag 103
|||||||||||||||||
Sbjct: 136 tccagatccagatccag 152
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 1715
Number of Sequences: 7669
Number of extensions: 1715
Number of successful extensions: 468
Number of sequences better than 0.5: 13
Number of HSP's better than 0.5 without gapping: 13
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 435
Number of HSP's gapped (non-prelim): 20
length of query: 868
length of database: 3,745,706
effective HSP length: 16
effective length of query: 852
effective length of database: 3,623,002
effective search space: 3086797704
effective search space used: 3086797704
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)