BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 131584.2.3
         (687 letters)

Database: MedicagoSativa_nucl_with_EST.fasta 
           7669 sequences; 3,745,706 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AJ410120.1|AJ410120  AJ410120 Medicago sativa library (Ka...   133   2e-031
gb|CO512972.1|CO512972  s13dSG09A0800053_121870 Glandular tr...    32   0.49 
>gb|AJ410120.1|AJ410120 AJ410120 Medicago sativa library (Kalo P) Medicago sativa cDNA
           clone U40, mRNA sequence
          Length = 757

 Score =  133 bits (67), Expect = 2e-031
 Identities = 209/255 (81%), Gaps = 1/255 (0%)
 Strand = Plus / Minus

                                                                       
Query: 335 aggaacatcttgctgatgaatct-atccttgttcacaggcagagccttctttttgccctt 393
           |||||||| ||||||||||| |  ||||||||| || ||| |||||||||| || || ||
Sbjct: 395 aggaacattttgctgatgaaacggatccttgttgactggctgagccttcttctttccttt 336

                                                                       
Query: 394 gccagtcttggggacctctgtccacatctcccgcacgttctcgagcaccatgttacagtg 453
            |||||||| || ||||| |||||||| ||||| || || || || ||||| || |||||
Sbjct: 335 accagtcttaggcacctcagtccacatttcccggacattttcaagaaccatattgcagtg 276

                                                                       
Query: 454 gcgatcaaaagcacgaacccggccaagcagcttcttgttgttgcggcaattgatgagaac 513
            | ||||||||| |  || ||||| || ||||| |||||||| || |||||||| |||||
Sbjct: 275 tctatcaaaagccctgacacggcctagaagctttttgttgttacgacaattgatcagaac 216

                                                                       
Query: 514 ctgggtgttgttcttaacactcatcattagtactgacagagggcctgtgctaaattcctc 573
           ||| |||||||| || ||||||||||| ||||| || || || || ||| | ||||||||
Sbjct: 215 ctgtgtgttgtttttcacactcatcatgagtacagatagtggaccagtgttgaattcctc 156

                          
Query: 574 ttcctctttcttccc 588
           |||||| ||||||||
Sbjct: 155 ttcctcattcttccc 141
>gb|CO512972.1|CO512972 s13dSG09A0800053_121870 Glandular trichomes Medicago sativa cDNA,
           mRNA sequence
          Length = 81

 Score = 32.2 bits (16), Expect = 0.49
 Identities = 16/16 (100%)
 Strand = Plus / Plus

                           
Query: 574 ttcctctttcttccca 589
           ||||||||||||||||
Sbjct: 50  ttcctctttcttccca 65
  Database: MedicagoSativa_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:23 PM
  Number of letters in database: 3,745,706
  Number of sequences in database:  7669
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 1248
Number of Sequences: 7669
Number of extensions: 1248
Number of successful extensions: 336
Number of sequences better than  0.5: 2
Number of HSP's better than  0.5 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 333
Number of HSP's gapped (non-prelim): 2
length of query: 687
length of database: 3,745,706
effective HSP length: 16
effective length of query: 671
effective length of database: 3,623,002
effective search space: 2431034342
effective search space used: 2431034342
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 16 (32.2 bits)