BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131543.2.1
(634 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO511931.1|CO511931 s13dSG05G0200020_103762 Glandular tr... 72 5e-013
gb|CO514409.1|CO514409 s13dSG36C0400024_327478 Glandular tr... 72 5e-013
gb|CO515008.1|CO515008 s13dSG26C0400034_397521 Glandular tr... 72 5e-013
gb|AF458700.1| Medicago sativa putative ankyrin-kinase (apk... 36 0.029
gb|CO517210.1|CO517210 s13dSG32A1000081_486016 Glandular tr... 32 0.45
gb|AF355596.1| Medicago sativa nodule clone MsNc20a mRNA, c... 32 0.45
>gb|CO511931.1|CO511931 s13dSG05G0200020_103762 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 464
Score = 71.9 bits (36), Expect = 5e-013
Identities = 117/144 (81%)
Strand = Plus / Plus
Query: 129 atggccaagtcgaagaaccacacggcgcacaaccagtcgttcaaggcgcacaagaacggc 188
||||| || |||||||| ||||| || ||||||||||| | ||| || |||||||| |||
Sbjct: 46 atggcgaaatcgaagaatcacaccgctcacaaccagtcttacaaagctcacaagaatggc 105
Query: 189 attaagaagcccaagcgccaccgccagacctccaccaaggggatggaccccaagttcctg 248
|| |||||||| ||| | || || || || || ||||| |||||||| || ||||| ||
Sbjct: 106 atcaagaagccaaagaggcatcgtcacacttcaaccaaagggatggatccaaagtttttg 165
Query: 249 aggaacctgaggtattctaggaag 272
||||||| ||||||| | ||||||
Sbjct: 166 aggaaccagaggtatgcaaggaag 189
>gb|CO514409.1|CO514409 s13dSG36C0400024_327478 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 367
Score = 71.9 bits (36), Expect = 5e-013
Identities = 117/144 (81%)
Strand = Plus / Plus
Query: 129 atggccaagtcgaagaaccacacggcgcacaaccagtcgttcaaggcgcacaagaacggc 188
||||| || |||||||| ||||| || ||||||||||| | ||| || |||||||| |||
Sbjct: 46 atggcgaaatcgaagaatcacaccgctcacaaccagtcttacaaagctcacaagaatggc 105
Query: 189 attaagaagcccaagcgccaccgccagacctccaccaaggggatggaccccaagttcctg 248
|| |||||||| ||| | || || || || || ||||| |||||||| || ||||| ||
Sbjct: 106 atcaagaagccaaagaggcatcgtcacacttcaaccaaagggatggatccaaagtttttg 165
Query: 249 aggaacctgaggtattctaggaag 272
||||||| ||||||| | ||||||
Sbjct: 166 aggaaccagaggtatgcaaggaag 189
>gb|CO515008.1|CO515008 s13dSG26C0400034_397521 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 367
Score = 71.9 bits (36), Expect = 5e-013
Identities = 117/144 (81%)
Strand = Plus / Plus
Query: 129 atggccaagtcgaagaaccacacggcgcacaaccagtcgttcaaggcgcacaagaacggc 188
||||| || |||||||| ||||| || ||||||||||| | ||| || |||||||| |||
Sbjct: 46 atggcgaaatcgaagaatcacaccgctcacaaccagtcttacaaagctcacaagaatggc 105
Query: 189 attaagaagcccaagcgccaccgccagacctccaccaaggggatggaccccaagttcctg 248
|| |||||||| ||| | || || || || || ||||| |||||||| || ||||| ||
Sbjct: 106 atcaagaagccaaagaggcatcgtcacacttcaaccaaagggatggatccaaagtttttg 165
Query: 249 aggaacctgaggtattctaggaag 272
||||||| ||||||| | ||||||
Sbjct: 166 aggaaccagaggtatgcaaggaag 189
>gb|AF458700.1| Medicago sativa putative ankyrin-kinase (apk1) mRNA, complete cds
Length = 1815
Score = 36.2 bits (18), Expect = 0.029
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 489 aattcggcacgaggctcg 506
||||||||||||||||||
Sbjct: 155 aattcggcacgaggctcg 172
>gb|CO517210.1|CO517210 s13dSG32A1000081_486016 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 624
Score = 32.2 bits (16), Expect = 0.45
Identities = 16/16 (100%)
Strand = Plus / Plus
Query: 433 gaaggttatgttttga 448
||||||||||||||||
Sbjct: 482 gaaggttatgttttga 497
>gb|AF355596.1| Medicago sativa nodule clone MsNc20a mRNA, complete sequence
Length = 1776
Score = 32.2 bits (16), Expect = 0.45
Identities = 19/20 (95%)
Strand = Plus / Minus
Query: 483 gtgcctaattcggcacgagg 502
||||| ||||||||||||||
Sbjct: 1172 gtgccgaattcggcacgagg 1153
Score = 32.2 bits (16), Expect = 0.45
Identities = 22/24 (91%)
Strand = Plus / Plus
Query: 478 accttgtgcctaattcggcacgag 501
|||| ||||| |||||||||||||
Sbjct: 1152 acctcgtgccgaattcggcacgag 1175
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 1540
Number of Sequences: 7669
Number of extensions: 1540
Number of successful extensions: 335
Number of sequences better than 0.5: 6
Number of HSP's better than 0.5 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 325
Number of HSP's gapped (non-prelim): 10
length of query: 634
length of database: 3,745,706
effective HSP length: 16
effective length of query: 618
effective length of database: 3,623,002
effective search space: 2239015236
effective search space used: 2239015236
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 16 (32.2 bits)