BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.280
(3445 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF049487.1|AF049487 Medicago sativa sucrose synthase mRN... 105 2e-022
gb|CO511826.1|CO511826 s13dSG02D1200102_103552 Glandular tr... 103 8e-022
gb|CO513133.1|CO513133 s13dSG23C0500034_129430 Glandular tr... 103 8e-022
gb|CO515699.1|CO515699 s13dSG60G0600052_419547 Glandular tr... 58 4e-008
gb|CO517054.1|CO517054 s13dSG31G0500037_468654 Glandular tr... 54 7e-007
gb|CO514282.1|CO514282 s13dSG76F0500043_157226 Glandular tr... 48 4e-005
gb|DQ122788.1| Medicago sativa clone B11 sucrose synthase m... 48 4e-005
gb|CO512297.1|CO512297 s13dSG68C1000070_114108 Glandular tr... 36 0.16
gb|CO514900.1|CO514900 s13dSG56G0300024_328460 Glandular tr... 36 0.16
gb|CO515105.1|CO515105 s13dSG40C0500038_399075 Glandular tr... 36 0.16
gb|DQ122842.1| Medicago sativa clone MF8 sucrose synthase m... 36 0.16
>gb|AF049487.1|AF049487 Medicago sativa sucrose synthase mRNA, complete cds
Length = 2760
Score = 105 bits (53), Expect = 2e-022
Identities = 233/293 (79%)
Strand = Plus / Plus
Query: 1364 gtcactcagtgtaccatcgctcatgccttggagaaaaccaaataccccaactcggacata 1423
||||||||||||||||| |||||||| | ||||| || || |||||| | || || ||
Sbjct: 1346 gtcactcagtgtaccattgctcatgcactcgagaagactaagtaccccgaatccgatatt 1405
Query: 1424 tacttggacaaattcgacagccagtaccacttctcttgccagttcacagctgaccttatt 1483
|||| | | |||||||| |||| |||||||| ||||| || || ||||| ||| |
Sbjct: 1406 tactggaaaaaattcgaagagaagtatcacttctcctgccaatttaccgctgatcttttc 1465
Query: 1484 gccatgaaccacaccgatttcatcatcaccagcacattccaagaaatcgcgggaagcaag 1543
|| ||||||||||| |||||||||||||| || || |||||||| || || |||||||||
Sbjct: 1466 gcaatgaaccacacagatttcatcatcactagtaccttccaagagattgctggaagcaag 1525
Query: 1544 gacaccgtggggcagtacgagtcccacatcgcgttcactcttcctgggctctaccgtgtc 1603
|| | || || ||||| ||| |||| || || |||||||| || ||||||||||||
Sbjct: 1526 gataaggttggacagtatgagagtcacactgcctttactcttccaggactctaccgtgtc 1585
Query: 1604 gtccatggcatcgatgttttcgatcccaagttcaacattgtctctcctggagc 1656
|| || || || ||||| || ||||| |||||||||||||| ||||| |||||
Sbjct: 1586 gtgcacggtattgatgtctttgatccaaagttcaacattgtatctccaggagc 1638
Score = 77.8 bits (39), Expect = 5e-014
Identities = 81/95 (85%)
Strand = Plus / Plus
Query: 1167 ttcgcgttcccttcagaaatgagaatggcatcctccgcaagtggatctctcgttttgatg 1226
|||| |||||||||||| ||||||| || || | |||||||||||||| || ||||| |
Sbjct: 1149 ttcgagttcccttcagagatgagaagggaattgttcgcaagtggatctcacgatttgaag 1208
Query: 1227 tctggccatacctggagacatacactgaggatgtt 1261
|||||||||| || || || |||||||||||||||
Sbjct: 1209 tctggccatatctagaaacttacactgaggatgtt 1243
Score = 67.9 bits (34), Expect = 5e-011
Identities = 100/122 (81%)
Strand = Plus / Plus
Query: 911 aatgttgttatcctttctcctcatggctacttcgctcagtccaatgtgcttggataccct 970
|||||||| ||||||||||||||||| ||||| ||||| |||| | |||||||||
Sbjct: 893 aatgttgtcatcctttctcctcatggttactttgctcaagatgatgtcttgggataccct 952
Query: 971 gacactggcggtcaggttgtgtacattctggatcaggtccgtgctttggagaatgagatg 1030
|| ||||| || |||||||| ||||| ||||||| || ||||| ||||||| ||||||
Sbjct: 953 gatactggaggccaggttgtttacatcttggatcaagttcgtgccttggagagcgagatg 1012
Query: 1031 ct 1032
||
Sbjct: 1013 ct 1014
Score = 48.1 bits (24), Expect = 4e-005
Identities = 102/128 (79%)
Strand = Plus / Plus
Query: 1979 tacaagttgaagggccatatccggtggatctcggcgcagatgaaccgcgtccgcaacggg 2038
||||||||||| ||||| || | ||||| || | ||||||||||| ||| | |||||
Sbjct: 1961 tacaagttgaatggccaattcagatggatttcctctcagatgaaccgtgtcagaaacgga 2020
Query: 2039 gagctgtaccgctacatttgcgataccaagggcgcattcgtgcagcctgcgttctacgaa 2098
||||||||||| ||||| || |||||||| || |||||||| ||||| | ||||||
Sbjct: 2021 gagctgtaccgtgtgatttgtgacaccaagggagctttcgtgcaacctgccgtgtacgaa 2080
Query: 2099 gcgttcgg 2106
|| |||||
Sbjct: 2081 gctttcgg 2088
Score = 44.1 bits (22), Expect = 7e-004
Identities = 22/22 (100%)
Strand = Plus / Plus
Query: 2160 atggtggccctgctgagatcat 2181
||||||||||||||||||||||
Sbjct: 2142 atggtggccctgctgagatcat 2163
Score = 42.1 bits (21), Expect = 0.003
Identities = 36/41 (87%)
Strand = Plus / Plus
Query: 637 ctacaagggcacgacgatgatgttgaatgacagaatccaaa 677
||||||||| | ||| ||||||||||||||||||| ||||
Sbjct: 619 ctacaagggaaagacattgatgttgaatgacagaattcaaa 659
Score = 38.2 bits (19), Expect = 0.040
Identities = 40/47 (85%)
Strand = Plus / Plus
Query: 1817 gaccgcgtgaagaacatgacaggcctggtcgagatgtacggcaagaa 1863
||||| ||||||||||| ||||| || || ||| ||||||||||||
Sbjct: 1799 gaccgtgtgaagaacattacaggacttgtggagtggtacggcaagaa 1845
Score = 38.2 bits (19), Expect = 0.040
Identities = 34/39 (87%)
Strand = Plus / Plus
Query: 302 caggaagcaattgtgctccccccatgggttgcacttgct 340
||||||||||| || | || ||||||||||||||||||
Sbjct: 284 caggaagcaatagttttgccaccatgggttgcacttgct 322
>gb|CO511826.1|CO511826 s13dSG02D1200102_103552 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 334
Score = 103 bits (52), Expect = 8e-022
Identities = 67/72 (93%)
Strand = Plus / Plus
Query: 2875 gccaagtggaagaagaagcgcatgaggaggctcaagaggaagcgcagaaagatgaggcag 2934
|||||||||||||||||||| ||||||||| | |||||||||||| |||||||||| |||
Sbjct: 80 gccaagtggaagaagaagcgtatgaggaggttgaagaggaagcgccgaaagatgagacag 139
Query: 2935 agatccaagtag 2946
||||||||||||
Sbjct: 140 agatccaagtag 151
>gb|CO513133.1|CO513133 s13dSG23C0500034_129430 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 337
Score = 103 bits (52), Expect = 8e-022
Identities = 67/72 (93%)
Strand = Plus / Plus
Query: 2875 gccaagtggaagaagaagcgcatgaggaggctcaagaggaagcgcagaaagatgaggcag 2934
|||||||||||||||||||| ||||||||| | |||||||||||| |||||||||| |||
Sbjct: 80 gccaagtggaagaagaagcgtatgaggaggttgaagaggaagcgccgaaagatgagacag 139
Query: 2935 agatccaagtag 2946
||||||||||||
Sbjct: 140 agatccaagtag 151
>gb|CO515699.1|CO515699 s13dSG60G0600052_419547 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 449
Score = 58.0 bits (29), Expect = 4e-008
Identities = 56/65 (86%)
Strand = Plus / Plus
Query: 1817 gaccgcgtgaagaacatgacaggcctggtcgagatgtacggcaagaacgcgcgcctgagg 1876
||||| ||||||||||| ||||| || || ||| ||||||||||||||| || ||||||
Sbjct: 126 gaccgagtgaagaacattacaggacttgttgagtggtacggcaagaacgctcgtctgagg 185
Query: 1877 gagct 1881
|||||
Sbjct: 186 gagct 190
>gb|CO517054.1|CO517054 s13dSG31G0500037_468654 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 374
Score = 54.0 bits (27), Expect = 7e-007
Identities = 60/71 (84%)
Strand = Plus / Plus
Query: 962 ggataccctgacactggcggtcaggttgtgtacattctggatcaggtccgtgctttggag 1021
||||||||||| || || || ||||||||||||||| |||| || || || || ||||||
Sbjct: 157 ggataccctgataccggtggacaggttgtgtacattttggaccaagttcgagccttggag 216
Query: 1022 aatgagatgct 1032
|| ||||||||
Sbjct: 217 aacgagatgct 227
>gb|CO514282.1|CO514282 s13dSG76F0500043_157226 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 515
Score = 48.1 bits (24), Expect = 4e-005
Identities = 27/28 (96%)
Strand = Plus / Plus
Query: 2156 tgccatggtggccctgctgagatcatcg 2183
||||||||||| ||||||||||||||||
Sbjct: 439 tgccatggtggtcctgctgagatcatcg 466
>gb|DQ122788.1| Medicago sativa clone B11 sucrose synthase mRNA, partial cds
Length = 536
Score = 48.1 bits (24), Expect = 4e-005
Identities = 27/28 (96%)
Strand = Plus / Plus
Query: 2156 tgccatggtggccctgctgagatcatcg 2183
||||||||||| ||||||||||||||||
Sbjct: 401 tgccatggtggtcctgctgagatcatcg 428
>gb|CO512297.1|CO512297 s13dSG68C1000070_114108 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 402
Score = 36.2 bits (18), Expect = 0.16
Identities = 18/18 (100%)
Strand = Plus / Minus
Query: 1503 tcatcatcaccagcacat 1520
||||||||||||||||||
Sbjct: 395 tcatcatcaccagcacat 378
>gb|CO514900.1|CO514900 s13dSG56G0300024_328460 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 411
Score = 36.2 bits (18), Expect = 0.16
Identities = 18/18 (100%)
Strand = Plus / Minus
Query: 1503 tcatcatcaccagcacat 1520
||||||||||||||||||
Sbjct: 401 tcatcatcaccagcacat 384
>gb|CO515105.1|CO515105 s13dSG40C0500038_399075 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 205
Score = 36.2 bits (18), Expect = 0.16
Identities = 21/22 (95%)
Strand = Plus / Plus
Query: 2925 gatgaggcagagatccaagtag 2946
|||||| |||||||||||||||
Sbjct: 4 gatgagacagagatccaagtag 25
>gb|DQ122842.1| Medicago sativa clone MF8 sucrose synthase mRNA, partial cds
Length = 652
Score = 36.2 bits (18), Expect = 0.16
Identities = 42/50 (84%)
Strand = Plus / Plus
Query: 467 aactttgtgcttgagcttgattttgagcccttcaatgcctcctttcctcg 516
||||||||||||||| | || ||||| || ||||| ||| | ||||||||
Sbjct: 575 aactttgtgcttgagttggactttgaaccattcaacgccccttttcctcg 624
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 5818
Number of Sequences: 7669
Number of extensions: 5818
Number of successful extensions: 1653
Number of sequences better than 0.5: 11
Number of HSP's better than 0.5 without gapping: 11
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1618
Number of HSP's gapped (non-prelim): 35
length of query: 3445
length of database: 3,745,706
effective HSP length: 17
effective length of query: 3428
effective length of database: 3,615,333
effective search space: 12393361524
effective search space used: 12393361524
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 18 (36.2 bits)