BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCS9f07.yg.2.1
(394 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM443222.2|BM443222 EBro02_SQ003_D03_R root, 3 week, hyd... 280 3e-074
gb|BM099511.2|BM099511 EBes01_SQ003_H09_R embryo sac, 4-6 D... 226 4e-058
gb|CA016096.1|CA016096 HV10C18u HV Hordeum vulgare subsp. v... 220 3e-056
gb|CA016336.1|CA016336 HV09G16u HV Hordeum vulgare subsp. v... 188 9e-047
gb|CA005005.1|CA005005 HU12K09u HU Hordeum vulgare subsp. v... 180 2e-044
gb|AL500587.1|AL500587 AL500587 Hordeum vulgare Barke roots... 139 8e-032
gb|CA008944.1|CA008944 HU12K09r HU Hordeum vulgare subsp. v... 74 4e-012
gb|BJ464838.1|BJ464838 BJ464838 K. Sato unpublished cDNA li... 56 9e-007
gb|BF259914.2|BF259914 HVSMEf0020J18f Hordeum vulgare seedl... 36 0.82
gb|AL505060.1|AL505060 AL505060 Hordeum vulgare Barke roots... 34 3.2
gb|AV935548.1|AV935548 AV935548 K. Sato unpublished cDNA li... 34 3.2
gb|BJ467326.1|BJ467326 BJ467326 K. Sato unpublished cDNA li... 34 3.2
gb|BJ474224.1|BJ474224 BJ474224 K. Sato unpublished cDNA li... 34 3.2
gb|BJ476246.1|BJ476246 BJ476246 K. Sato unpublished cDNA li... 34 3.2
gb|BJ476250.1|BJ476250 BJ476250 K. Sato unpublished cDNA li... 34 3.2
gb|BJ477286.1|BJ477286 BJ477286 K. Sato unpublished cDNA li... 34 3.2
gb|BQ657350.1|BQ657350 HA08L13u HA Hordeum vulgare subsp. v... 34 3.2
gb|CA012317.1|CA012317 HT05B12r HT Hordeum vulgare subsp. v... 34 3.2
gb|CA012327.1|CA012327 HT05B22r HT Hordeum vulgare subsp. v... 34 3.2
gb|CA020612.1|CA020612 HZ36N19r HZ Hordeum vulgare subsp. v... 34 3.2
gb|CD663518.1|CD663518 UCRHV18_05da05_b1 Drought-stressed D... 34 3.2
>gb|BM443222.2|BM443222 EBro02_SQ003_D03_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ003_D03 5', mRNA sequence
Length = 383
Score = 280 bits (141), Expect = 3e-074
Identities = 291/341 (85%)
Strand = Plus / Minus
Query: 54 tcatccccacctctaatactcggtggcctttcatccttccatacaggaacgtggttgagg 113
||||||||||||| |||||| | |||||||||||||||||||| |||||| || ||||||
Sbjct: 352 tcatccccacctcgaatacttgatggcctttcatccttccatataggaacatgcttgagg 293
Query: 114 gacctaaatttaccgagcactgcagattccaaggctcccagtgttaactggcccgatctc 173
|| ||||||||| || |||| ||||| | ||| ||||| | |||||||| | |||||
Sbjct: 292 gagctaaatttatcgtgcacagcagacttcaaagctcctaaagttaactgatctgatctt 233
Query: 174 atataaatgctctctgacttaaaccgccctctctctcggataaacaagtggtaaactgtc 233
| ||||||| |||||| |||||||||||||||||||| || ||| |||||||| |||||
Sbjct: 232 agataaatgttctctgtcttaaaccgccctctctctctgacaaataagtggtatactgtt 173
Query: 234 ccctttttgctggaacagctacaaggtcgatttgtaaattttggagtcctttcctccttt 293
|| |||||||||| || ||||| | ||||| ||||||||||||| |||| || |||
Sbjct: 172 cctcttttgctggagcaactacacgagcgattcataaattttggagttttttcttctttt 113
Query: 294 aaatgaatcttggtagcaatatgtatacaaagacgacaagatgaatgaaccgctgccatg 353
||||||||| ||||| |||||||||||||| ||||| |||||||||||||||||||||
Sbjct: 112 tcatgaatcttcgtagcgatatgtatacaaaggcgacaggatgaatgaaccgctgccatg 53
Query: 354 tcgataagagctttgaaagaatcagatggaccatcatactt 394
|| ||||| || |||||||||||||||||||||||||||||
Sbjct: 52 tcaataagtgccttgaaagaatcagatggaccatcatactt 12
>gb|BM099511.2|BM099511 EBes01_SQ003_H09_R embryo sac, 4-6 DPA, no treatment, cv Optic,
EBes01 Hordeum vulgare subsp. vulgare cDNA clone
EBes01_SQ003_H09 5', mRNA sequence
Length = 689
Score = 226 bits (114), Expect = 4e-058
Identities = 255/302 (84%)
Strand = Plus / Minus
Query: 93 catacaggaacgtggttgagggacctaaatttaccgagcactgcagattccaaggctccc 152
|||| |||||| || |||||||| ||||||||| || |||| ||||| | ||| |||||
Sbjct: 689 catataggaacatgcttgagggagctaaatttatcgtgcacagcagacttcaaagctcct 630
Query: 153 agtgttaactggcccgatctcatataaatgctctctgacttaaaccgccctctctctcgg 212
| |||||||| | ||||| | ||||||| |||||| |||||||||||||||||||| |
Sbjct: 629 aaagttaactgatctgatcttagataaatgttctctgtcttaaaccgccctctctctctg 570
Query: 213 ataaacaagtggtaaactgtcccctttttgctggaacagctacaaggtcgatttgtaaat 272
| ||| |||||||| ||||| || |||||||||| || ||||| | ||||| |||||
Sbjct: 569 acaaataagtggtatactgttcctcttttgctggagcaactacacgagcgattcataaat 510
Query: 273 tttggagtcctttcctcctttaaatgaatcttggtagcaatatgtatacaaagacgacaa 332
|||||||| |||| || ||| ||||||||| ||||| |||||||||||||| |||||
Sbjct: 509 tttggagttttttcttctttttcatgaatcttcgtagcgatatgtatacaaaggcgacag 450
Query: 333 gatgaatgaaccgctgccatgtcgataagagctttgaaagaatcagatggaccatcatac 392
||||||||||||||||||||||| ||||| || |||||||||||||||||||||||||||
Sbjct: 449 gatgaatgaaccgctgccatgtcaataagtgccttgaaagaatcagatggaccatcatac 390
Query: 393 tt 394
||
Sbjct: 389 tt 388
>gb|CA016096.1|CA016096 HV10C18u HV Hordeum vulgare subsp. vulgare cDNA clone HV10C18
3-PRIME, mRNA sequence
Length = 556
Score = 220 bits (111), Expect = 3e-056
Identities = 252/299 (84%)
Strand = Plus / Plus
Query: 54 tcatccccacctctaatactcggtggcctttcatccttccatacaggaacgtggttgagg 113
||||||||||||| |||||| | |||||||||||||||||||| |||||| || ||||||
Sbjct: 258 tcatccccacctcgaatacttgatggcctttcatccttccatataggaacatgcttgagg 317
Query: 114 gacctaaatttaccgagcactgcagattccaaggctcccagtgttaactggcccgatctc 173
|| ||||||||| || |||| ||||| | ||| ||||| | |||||||| | |||||
Sbjct: 318 gagctaaatttatcgtgcacagcagacttcaaagctcctaaagttaactgatctgatctt 377
Query: 174 atataaatgctctctgacttaaaccgccctctctctcggataaacaagtggtaaactgtc 233
| ||||||| |||||| |||||||||||||||||||| || ||| |||||||| |||||
Sbjct: 378 agataaatgttctctgtcttaaaccgccctctctctctgacaaataagtggtatactgtt 437
Query: 234 ccctttttgctggaacagctacaaggtcgatttgtaaattttggagtcctttcctccttt 293
|| |||||||||| || ||||| | ||||| ||||||||||||| |||| || |||
Sbjct: 438 cctcttttgctggagcaactacacgagcgattcataaattttggagttttttcttctttt 497
Query: 294 aaatgaatcttggtagcaatatgtatacaaagacgacaagatgaatgaaccgctgccat 352
||||||||| ||||| |||||||||||||| ||||| ||||||||||||||||||||
Sbjct: 498 tcatgaatcttcgtagcgatatgtatacaaaggcgacaggatgaatgaaccgctgccat 556
>gb|CA016336.1|CA016336 HV09G16u HV Hordeum vulgare subsp. vulgare cDNA clone HV09G16
3-PRIME, mRNA sequence
Length = 540
Score = 188 bits (95), Expect = 9e-047
Identities = 236/283 (83%)
Strand = Plus / Plus
Query: 54 tcatccccacctctaatactcggtggcctttcatccttccatacaggaacgtggttgagg 113
||||||||||||| |||||| | |||||||||||||||||||| |||||| || ||||||
Sbjct: 258 tcatccccacctcgaatacttgatggcctttcatccttccatataggaacatgcttgagg 317
Query: 114 gacctaaatttaccgagcactgcagattccaaggctcccagtgttaactggcccgatctc 173
|| ||||||||| || |||| ||||| | ||| ||||| | |||||||| | |||||
Sbjct: 318 gagctaaatttatcgtgcacagcagacttcaaagctcctaaagttaactgatctgatctt 377
Query: 174 atataaatgctctctgacttaaaccgccctctctctcggataaacaagtggtaaactgtc 233
| ||||||| |||||| |||||||||||||||||||| || ||| |||||||| |||||
Sbjct: 378 agataaatgttctctgtcttaaaccgccctctctctctgacaaataagtggtatactgtt 437
Query: 234 ccctttttgctggaacagctacaaggtcgatttgtaaattttggagtcctttcctccttt 293
|| |||||||||| || ||||| | ||||| ||||||||||||| |||| || |||
Sbjct: 438 cctcttttgctggagcaactacacgagcgattcataaattttggagttttttcttctttt 497
Query: 294 aaatgaatcttggtagcaatatgtatacaaagacgacaagatg 336
||||||||| ||||| |||||||||||||| ||||| ||||
Sbjct: 498 tcatgaatcttcgtagcgatatgtatacaaaggcgacaggatg 540
>gb|CA005005.1|CA005005 HU12K09u HU Hordeum vulgare subsp. vulgare cDNA clone HU12K09
3-PRIME, mRNA sequence
Length = 543
Score = 180 bits (91), Expect = 2e-044
Identities = 239/287 (83%), Gaps = 1/287 (0%)
Strand = Plus / Plus
Query: 54 tcatccccacctctaatactcggtggcctttcatccttccatacaggaacgtggttgagg 113
||||||||||||| |||||| | |||||||||||||||||||| |||||| || ||||||
Sbjct: 257 tcatccccacctcgaatacttgatggcctttcatccttccatataggaacatgcttgagg 316
Query: 114 gacctaaatttaccgagcactgcagattccaaggctcccagtgttaactggcccgatctc 173
|| ||||||||| || |||| ||||| | ||| ||||| | |||||||| | |||||
Sbjct: 317 gagctaaatttatcgtgcacagcagacttcaaagctcctaaagttaactgatctgatctt 376
Query: 174 atataaatgctctctgacttaaaccgccctctctctcggataaacaagtggtaaactgtc 233
| ||||||| |||||| |||||||||||||||||||| || ||| |||||||| |||||
Sbjct: 377 agataaatgttctctgtcttaaaccgccctctctctctgacaaataagtggtatactgtt 436
Query: 234 ccctttttgctggaacagctacaaggtcgatttgtaaattttggagtcctttcctccttt 293
|| |||||||||| || ||||| | ||||| ||||||||||||| ||| || ||||
Sbjct: 437 cctcttttgctggagcaactacacgagcgattcataaattttggagtttttttcttcttt 496
Query: 294 aa-atgaatcttggtagcaatatgtatacaaagacgacaagatgaat 339
||||||||| ||||| |||||||||||||| ||||| |||||||
Sbjct: 497 ttcatgaatcttcgtagcgatatgtatacaaaggcgacaggatgaat 543
>gb|AL500587.1|AL500587 AL500587 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW01C14u 3', mRNA sequence
Length = 526
Score = 139 bits (70), Expect = 8e-032
Identities = 162/193 (83%)
Strand = Plus / Plus
Query: 54 tcatccccacctctaatactcggtggcctttcatccttccatacaggaacgtggttgagg 113
||||||||||||| |||||| | |||||||||||||||||||| |||||| || ||||||
Sbjct: 299 tcatccccacctcgaatacttgatggcctttcatccttccatataggaacatgcttgagg 358
Query: 114 gacctaaatttaccgagcactgcagattccaaggctcccagtgttaactggcccgatctc 173
|| ||||||||| || |||| ||||| | ||| ||||| | |||||||| | |||||
Sbjct: 359 gagctaaatttatcgtgcacagcagacttcaaagctcctaaagttaactgatctgatctt 418
Query: 174 atataaatgctctctgacttaaaccgccctctctctcggataaacaagtggtaaactgtc 233
| ||||||| |||||| |||||||||||||||||||| || || |||||||| |||||
Sbjct: 419 agataaatgttctctgtcttaaaccgccctctctctctgacaantaagtggtatactgtt 478
Query: 234 ccctttttgctgg 246
|| |||||||||
Sbjct: 479 cctcttttgctgg 491
>gb|CA008944.1|CA008944 HU12K09r HU Hordeum vulgare subsp. vulgare cDNA clone HU12K09
5-PRIME, mRNA sequence
Length = 551
Score = 73.8 bits (37), Expect = 4e-012
Identities = 46/49 (93%)
Strand = Plus / Minus
Query: 346 ctgccatgtcgataagagctttgaaagaatcagatggaccatcatactt 394
|||||||||| ||||| || |||||||||||||||||||||||||||||
Sbjct: 551 ctgccatgtcaataagtgccttgaaagaatcagatggaccatcatactt 503
>gb|BJ464838.1|BJ464838 BJ464838 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags36d24 5', mRNA sequence
Length = 571
Score = 56.0 bits (28), Expect = 9e-007
Identities = 28/28 (100%)
Strand = Plus / Minus
Query: 367 tgaaagaatcagatggaccatcatactt 394
||||||||||||||||||||||||||||
Sbjct: 571 tgaaagaatcagatggaccatcatactt 544
>gb|BF259914.2|BF259914 HVSMEf0020J18f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0020J18f, mRNA sequence
Length = 871
Score = 36.2 bits (18), Expect = 0.82
Identities = 18/18 (100%)
Strand = Plus / Minus
Query: 78 ggcctttcatccttccat 95
||||||||||||||||||
Sbjct: 559 ggcctttcatccttccat 542
>gb|AL505060.1|AL505060 AL505060 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW07E07V 5', mRNA sequence
Length = 435
Score = 34.2 bits (17), Expect = 3.2
Identities = 17/17 (100%)
Strand = Plus / Minus
Query: 367 tgaaagaatcagatgga 383
|||||||||||||||||
Sbjct: 333 tgaaagaatcagatgga 317
>gb|AV935548.1|AV935548 AV935548 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal4j22 3', mRNA sequence
Length = 640
Score = 34.2 bits (17), Expect = 3.2
Identities = 20/21 (95%)
Strand = Plus / Plus
Query: 362 agctttgaaagaatcagatgg 382
|||||||| ||||||||||||
Sbjct: 457 agctttgagagaatcagatgg 477
>gb|BJ467326.1|BJ467326 BJ467326 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags26k23 3', mRNA sequence
Length = 560
Score = 34.2 bits (17), Expect = 3.2
Identities = 20/21 (95%)
Strand = Plus / Plus
Query: 362 agctttgaaagaatcagatgg 382
|||||||| ||||||||||||
Sbjct: 375 agctttgagagaatcagatgg 395
>gb|BJ474224.1|BJ474224 BJ474224 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal19k14 3', mRNA sequence
Length = 598
Score = 34.2 bits (17), Expect = 3.2
Identities = 20/21 (95%)
Strand = Plus / Plus
Query: 362 agctttgaaagaatcagatgg 382
|||||||| ||||||||||||
Sbjct: 452 agctttgagagaatcagatgg 472
>gb|BJ476246.1|BJ476246 BJ476246 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal33a14 3', mRNA sequence
Length = 488
Score = 34.2 bits (17), Expect = 3.2
Identities = 20/21 (95%)
Strand = Plus / Plus
Query: 362 agctttgaaagaatcagatgg 382
|||||||| ||||||||||||
Sbjct: 427 agctttgagagaatcagatgg 447
>gb|BJ476250.1|BJ476250 BJ476250 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal33a18 3', mRNA sequence
Length = 685
Score = 34.2 bits (17), Expect = 3.2
Identities = 20/21 (95%)
Strand = Plus / Plus
Query: 362 agctttgaaagaatcagatgg 382
|||||||| ||||||||||||
Sbjct: 428 agctttgagagaatcagatgg 448
>gb|BJ477286.1|BJ477286 BJ477286 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal16h19 3', mRNA sequence
Length = 636
Score = 34.2 bits (17), Expect = 3.2
Identities = 20/21 (95%)
Strand = Plus / Plus
Query: 362 agctttgaaagaatcagatgg 382
|||||||| ||||||||||||
Sbjct: 453 agctttgagagaatcagatgg 473
>gb|BQ657350.1|BQ657350 HA08L13u HA Hordeum vulgare subsp. vulgare cDNA clone HA08L13
3-PRIME, mRNA sequence
Length = 572
Score = 34.2 bits (17), Expect = 3.2
Identities = 20/21 (95%)
Strand = Plus / Plus
Query: 362 agctttgaaagaatcagatgg 382
|||||||| ||||||||||||
Sbjct: 472 agctttgagagaatcagatgg 492
>gb|CA012317.1|CA012317 HT05B12r HT Hordeum vulgare subsp. vulgare cDNA clone HT05B12
5-PRIME, mRNA sequence
Length = 600
Score = 34.2 bits (17), Expect = 3.2
Identities = 20/21 (95%)
Strand = Plus / Minus
Query: 362 agctttgaaagaatcagatgg 382
|||||||| ||||||||||||
Sbjct: 392 agctttgagagaatcagatgg 372
>gb|CA012327.1|CA012327 HT05B22r HT Hordeum vulgare subsp. vulgare cDNA clone HT05B22
5-PRIME, mRNA sequence
Length = 546
Score = 34.2 bits (17), Expect = 3.2
Identities = 20/21 (95%)
Strand = Plus / Minus
Query: 362 agctttgaaagaatcagatgg 382
|||||||| ||||||||||||
Sbjct: 393 agctttgagagaatcagatgg 373
>gb|CA020612.1|CA020612 HZ36N19r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ36N19
5-PRIME, mRNA sequence
Length = 531
Score = 34.2 bits (17), Expect = 3.2
Identities = 20/21 (95%)
Strand = Plus / Minus
Query: 362 agctttgaaagaatcagatgg 382
|||||||| ||||||||||||
Sbjct: 476 agctttgagagaatcagatgg 456
>gb|CD663518.1|CD663518 UCRHV18_05da05_b1 Drought-stressed Dicktoo barley epidermis cDNA
library Hordeum vulgare subsp. vulgare cDNA clone
UCRHV18_05da05, mRNA sequence
Length = 714
Score = 34.2 bits (17), Expect = 3.2
Identities = 20/21 (95%)
Strand = Plus / Plus
Query: 362 agctttgaaagaatcagatgg 382
|||||||| ||||||||||||
Sbjct: 478 agctttgagagaatcagatgg 498
Database: Hordeum_nucl_with_EST.fasta
Posted date: May 2, 2006 2:38 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 28,115
Number of Sequences: 312970
Number of extensions: 28115
Number of successful extensions: 7248
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 7226
Number of HSP's gapped (non-prelim): 22
length of query: 394
length of database: 175,134,539
effective HSP length: 18
effective length of query: 376
effective length of database: 169,501,079
effective search space: 63732405704
effective search space used: 63732405704
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)