BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCK30g08.yg.2.1
(164 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE558753.3|BE558753 HV_CEb0020E08f Hordeum vulgare seedl... 54 1e-006
gb|BI960005.1|BI960005 HVSMEn0022N08f Hordeum vulgare rachi... 46 3e-004
gb|BJ463662.1|BJ463662 BJ463662 K. Sato unpublished cDNA li... 46 3e-004
gb|BG418510.1|BG418510 HVSMEk0022O21f Hordeum vulgare testa... 36 0.32
gb|BG414392.2|BG414392 HVSMEk0002A20f Hordeum vulgare testa... 36 0.32
gb|BG418408.2|BG418408 HVSMEk0022J14f Hordeum vulgare testa... 36 0.32
gb|AV910611.1|AV910611 AV910611 K. Sato unpublished cDNA li... 36 0.32
gb|AV911718.1|AV911718 AV911718 K. Sato unpublished cDNA li... 36 0.32
gb|BM928834.1|BM928834 LP10007 Lemma/palea-enriched cDNA li... 36 0.32
gb|BQ134683.1|BQ134683 LP30159 Lemma/palea-enriched cDNA li... 36 0.32
gb|BM376088.2|BM376088 EBma01_SQ002_F01_R maternal, 4 DPA, ... 36 0.32
gb|BM371429.2|BM371429 EBma08_SQ002_L04_R maternal, 28 DPA,... 36 0.32
gb|BU970810.1|BU970810 HB15M02r BC Hordeum vulgare subsp. v... 36 0.32
gb|BU987678.1|BU987678 HF15H05r HF Hordeum vulgare subsp. v... 36 0.32
>gb|BE558753.3|BE558753 HV_CEb0020E08f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0020E08f, mRNA sequence
Length = 790
Score = 54.0 bits (27), Expect = 1e-006
Identities = 42/47 (89%)
Strand = Plus / Plus
Query: 97 gatatctaccgttcagatcctgaatggaagatcattttacttaggta 143
||||| ||||| |||||| |||| |||| ||||||||||||||||||
Sbjct: 4 gatatataccggtcagattctgagtggaggatcattttacttaggta 50
>gb|BI960005.1|BI960005 HVSMEn0022N08f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0022N08f, mRNA sequence
Length = 628
Score = 46.1 bits (23), Expect = 3e-004
Identities = 26/27 (96%)
Strand = Plus / Plus
Query: 1 ggatcacccaaaaactcaccctgcaca 27
|||||||| ||||||||||||||||||
Sbjct: 531 ggatcacctaaaaactcaccctgcaca 557
>gb|BJ463662.1|BJ463662 BJ463662 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags32p11 5', mRNA sequence
Length = 540
Score = 46.1 bits (23), Expect = 3e-004
Identities = 54/63 (85%), Gaps = 1/63 (1%)
Strand = Plus / Plus
Query: 81 tgaggatatttgccgggatatctaccgttcagatcctgaatggaagatcattttacttag 140
|||||| || ||||| ||||| ||||| |||||| |||| |||| ||| |||||||||||
Sbjct: 13 tgaggagatatgccgtgatatataccggtcagattctgagtggaggat-attttacttag 71
Query: 141 gta 143
|||
Sbjct: 72 gta 74
>gb|BG418510.1|BG418510 HVSMEk0022O21f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0022O21f, mRNA sequence
Length = 495
Score = 36.2 bits (18), Expect = 0.32
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 111 agatcctgaatggaagat 128
||||||||||||||||||
Sbjct: 222 agatcctgaatggaagat 239
>gb|BG414392.2|BG414392 HVSMEk0002A20f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0002A20f, mRNA sequence
Length = 945
Score = 36.2 bits (18), Expect = 0.32
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 111 agatcctgaatggaagat 128
||||||||||||||||||
Sbjct: 254 agatcctgaatggaagat 271
>gb|BG418408.2|BG418408 HVSMEk0022J14f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0022J14f, mRNA sequence
Length = 885
Score = 36.2 bits (18), Expect = 0.32
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 111 agatcctgaatggaagat 128
||||||||||||||||||
Sbjct: 413 agatcctgaatggaagat 430
>gb|AV910611.1|AV910611 AV910611 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak1b15 5', mRNA sequence
Length = 500
Score = 36.2 bits (18), Expect = 0.32
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 111 agatcctgaatggaagat 128
||||||||||||||||||
Sbjct: 293 agatcctgaatggaagat 310
>gb|AV911718.1|AV911718 AV911718 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak1b15 3', mRNA sequence
Length = 666
Score = 36.2 bits (18), Expect = 0.32
Identities = 18/18 (100%)
Strand = Plus / Minus
Query: 111 agatcctgaatggaagat 128
||||||||||||||||||
Sbjct: 619 agatcctgaatggaagat 602
>gb|BM928834.1|BM928834 LP10007 Lemma/palea-enriched cDNA library from elongation stage of
kernel Hordeum vulgare subsp. vulgare cDNA clone 1-7,
mRNA sequence
Length = 420
Score = 36.2 bits (18), Expect = 0.32
Identities = 25/26 (96%), Gaps = 1/26 (3%)
Strand = Plus / Minus
Query: 140 ggtacctgcc-gggcggccgctcgaa 164
|||||||||| |||||||||||||||
Sbjct: 30 ggtacctgcccgggcggccgctcgaa 5
>gb|BQ134683.1|BQ134683 LP30159 Lemma/palea-enriched cDNA library from dough stage of
kernel Hordeum vulgare subsp. vulgare cDNA clone 3-159
similar to putative ABC transporter, mRNA sequence
Length = 339
Score = 36.2 bits (18), Expect = 0.32
Identities = 25/26 (96%), Gaps = 1/26 (3%)
Strand = Plus / Plus
Query: 140 ggtacctgcc-gggcggccgctcgaa 164
|||||||||| |||||||||||||||
Sbjct: 261 ggtacctgcccgggcggccgctcgaa 286
>gb|BM376088.2|BM376088 EBma01_SQ002_F01_R maternal, 4 DPA, no treatment, cv Optic, EBma01
Hordeum vulgare subsp. vulgare cDNA clone
EBma01_SQ002_F01 5', mRNA sequence
Length = 256
Score = 36.2 bits (18), Expect = 0.32
Identities = 25/26 (96%), Gaps = 1/26 (3%)
Strand = Plus / Minus
Query: 140 ggtacctgcc-gggcggccgctcgaa 164
|||||||||| |||||||||||||||
Sbjct: 104 ggtacctgcccgggcggccgctcgaa 79
>gb|BM371429.2|BM371429 EBma08_SQ002_L04_R maternal, 28 DPA, no treatment, cv Optic, EBma08
Hordeum vulgare subsp. vulgare cDNA clone
EBma08_SQ002_L04 5', mRNA sequence
Length = 454
Score = 36.2 bits (18), Expect = 0.32
Identities = 25/26 (96%), Gaps = 1/26 (3%)
Strand = Plus / Minus
Query: 140 ggtacctgcc-gggcggccgctcgaa 164
|||||||||| |||||||||||||||
Sbjct: 104 ggtacctgcccgggcggccgctcgaa 79
>gb|BU970810.1|BU970810 HB15M02r BC Hordeum vulgare subsp. vulgare cDNA clone HB15M02
5-PRIME, mRNA sequence
Length = 498
Score = 36.2 bits (18), Expect = 0.32
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 111 agatcctgaatggaagat 128
||||||||||||||||||
Sbjct: 152 agatcctgaatggaagat 169
>gb|BU987678.1|BU987678 HF15H05r HF Hordeum vulgare subsp. vulgare cDNA clone HF15H05
5-PRIME, mRNA sequence
Length = 667
Score = 36.2 bits (18), Expect = 0.32
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 111 agatcctgaatggaagat 128
||||||||||||||||||
Sbjct: 425 agatcctgaatggaagat 442
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 13,336
Number of Sequences: 312970
Number of extensions: 13336
Number of successful extensions: 2623
Number of sequences better than 0.5: 14
Number of HSP's better than 0.5 without gapping: 9
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 2614
Number of HSP's gapped (non-prelim): 14
length of query: 164
length of database: 175,134,539
effective HSP length: 18
effective length of query: 146
effective length of database: 169,501,079
effective search space: 24747157534
effective search space used: 24747157534
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 18 (36.2 bits)