BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAO3g02.yg.2.1
(344 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV911026.1|AV911026 AV911026 K. Sato unpublished cDNA li... 74 3e-012
gb|AL505025.1|AL505025 AL505025 Hordeum vulgare Barke roots... 72 1e-011
gb|AV936335.1|AV936335 AV936335 K. Sato unpublished cDNA li... 72 1e-011
gb|CB880964.1|CB880964 HM08F20w HM Hordeum vulgare subsp. v... 72 1e-011
gb|CB882449.1|CB882449 HL01O07w HL Hordeum vulgare subsp. v... 72 1e-011
gb|CD663353.1|CD663353 UCRHV18_05ah10_b1 Drought-stressed D... 72 1e-011
gb|BU987196.1|BU987196 HF14B07r HF Hordeum vulgare subsp. v... 64 3e-009
gb|BJ474523.1|BJ474523 BJ474523 K. Sato unpublished cDNA li... 60 5e-008
gb|AV928420.1|AV928420 AV928420 K. Sato unpublished cDNA li... 58 2e-007
gb|BQ664207.1|BQ664207 HV02F10u HV Hordeum vulgare subsp. v... 58 2e-007
gb|BM097758.2|BM097758 EBem04_SQ002_J20_R embryo, 12 DPA, n... 58 2e-007
gb|CA010449.1|CA010449 HT09N07u HT Hordeum vulgare subsp. v... 58 2e-007
gb|BU975768.1|BU975768 HB32D23r BC Hordeum vulgare subsp. v... 54 3e-006
>gb|AV911026.1|AV911026 AV911026 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak11m03 3', mRNA sequence
Length = 685
Score = 73.8 bits (37), Expect = 3e-012
Identities = 91/111 (81%)
Strand = Plus / Minus
Query: 211 atggtccgcatattatgcaacgactgcggggcaatatcagatgtgcagttccatttgatt 270
||||| |||||||| ||||| || |||||||| ||||||| ||||||||||| || |||
Sbjct: 566 atggttcgcatattgtgcaatgattgcggggcggtatcagaggtgcagttccacttaatt 507
Query: 271 gcgcacaagtgccagaatnnnnantcatacaacacccgcnagatttgaagt 321
|| || |||||||| | | | |||||||||||||| |||||||||||
Sbjct: 506 gcacataagtgccatagttgcaagtcatacaacacccggcagatttgaagt 456
>gb|AL505025.1|AL505025 AL505025 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW07C11V 5', mRNA sequence
Length = 478
Score = 71.9 bits (36), Expect = 1e-011
Identities = 96/117 (82%)
Strand = Plus / Plus
Query: 15 gtacttgttcgattcgacaaatgatgtttccgtcctgccttnnngtcataccattcatgt 74
||||||||| || |||| ||||||||| || || ||||| | ||||||| |||||||
Sbjct: 290 gtacttgtttgagtcgagaaatgatgtctctgttctgccgtgtggtcatacaattcatga 349
Query: 75 aaagtgcctgaaagaaatggaagagcactgccagtttgcatgccccctttgctccaa 131
||| ||| ||||||||||| | ||||| ||||| || |||||||| || ||||||||
Sbjct: 350 aaaatgcttgaaagaaatgaaggagcattgccaattcgcatgcccgctctgctccaa 406
>gb|AV936335.1|AV936335 AV936335 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal10p08 3', mRNA sequence
Length = 678
Score = 71.9 bits (36), Expect = 1e-011
Identities = 96/117 (82%)
Strand = Plus / Minus
Query: 15 gtacttgttcgattcgacaaatgatgtttccgtcctgccttnnngtcataccattcatgt 74
||||||||| || |||| ||||||||| || || ||||| | ||||||| |||||||
Sbjct: 482 gtacttgtttgagtcgagaaatgatgtctctgttctgccgtgtggtcatacaattcatga 423
Query: 75 aaagtgcctgaaagaaatggaagagcactgccagtttgcatgccccctttgctccaa 131
||| ||| ||||||||||| | ||||| ||||| || |||||||| || ||||||||
Sbjct: 422 aaaatgcttgaaagaaatgaaggagcattgccaattcgcatgcccgctctgctccaa 366
Score = 58.0 bits (29), Expect = 2e-007
Identities = 89/111 (80%)
Strand = Plus / Minus
Query: 211 atggtccgcatattatgcaacgactgcggggcaatatcagatgtgcagttccatttgatt 270
||||| |||||||| ||||| || |||||||| ||||||| |||||||| || || |||
Sbjct: 286 atggttcgcatattgtgcaatgattgcggggcggtatcagaggtgcagtttcacttaatt 227
Query: 271 gcgcacaagtgccagaatnnnnantcatacaacacccgcnagatttgaagt 321
|| || |||||||| | | | |||||||||||||| | |||||||||
Sbjct: 226 gcacataagtgccatagttgcaagtcatacaacacccggcaaatttgaagt 176
>gb|CB880964.1|CB880964 HM08F20w HM Hordeum vulgare subsp. vulgare cDNA clone HM08F20
3-PRIME, mRNA sequence
Length = 663
Score = 71.9 bits (36), Expect = 1e-011
Identities = 96/117 (82%)
Strand = Plus / Minus
Query: 15 gtacttgttcgattcgacaaatgatgtttccgtcctgccttnnngtcataccattcatgt 74
||||||||| || |||| ||||||||| || || ||||| | ||||||| |||||||
Sbjct: 561 gtacttgtttgagtcgagaaatgatgtctctgttctgccgtgtggtcatacaattcatga 502
Query: 75 aaagtgcctgaaagaaatggaagagcactgccagtttgcatgccccctttgctccaa 131
||| ||| ||||||||||| | ||||| ||||| || |||||||| || ||||||||
Sbjct: 501 aaaatgcttgaaagaaatgaaggagcattgccaattcgcatgcccgctctgctccaa 445
Score = 58.0 bits (29), Expect = 2e-007
Identities = 89/111 (80%)
Strand = Plus / Minus
Query: 211 atggtccgcatattatgcaacgactgcggggcaatatcagatgtgcagttccatttgatt 270
||||| |||||||| ||||| || |||||||| ||||||| |||||||| || || |||
Sbjct: 365 atggttcgcatattgtgcaatgattgcggggcggtatcagaggtgcagtttcacttaatt 306
Query: 271 gcgcacaagtgccagaatnnnnantcatacaacacccgcnagatttgaagt 321
|| || |||||||| | | | |||||||||||||| | |||||||||
Sbjct: 305 gcacataagtgccatagttgcaagtcatacaacacccgacaaatttgaagt 255
>gb|CB882449.1|CB882449 HL01O07w HL Hordeum vulgare subsp. vulgare cDNA clone HL01O07
3-PRIME, mRNA sequence
Length = 593
Score = 71.9 bits (36), Expect = 1e-011
Identities = 96/117 (82%)
Strand = Plus / Plus
Query: 15 gtacttgttcgattcgacaaatgatgtttccgtcctgccttnnngtcataccattcatgt 74
||||||||| || |||| ||||||||| || || ||||| | ||||||| |||||||
Sbjct: 195 gtacttgtttgagtcgagaaatgatgtctctgttctgccgtgtggtcatacaattcatga 254
Query: 75 aaagtgcctgaaagaaatggaagagcactgccagtttgcatgccccctttgctccaa 131
||| ||| ||||||||||| | ||||| ||||| || |||||||| || ||||||||
Sbjct: 255 aaaatgcttgaaagaaatgaaggagcattgccaattcgcatgcccgctctgctccaa 311
Score = 58.0 bits (29), Expect = 2e-007
Identities = 89/111 (80%)
Strand = Plus / Plus
Query: 211 atggtccgcatattatgcaacgactgcggggcaatatcagatgtgcagttccatttgatt 270
||||| |||||||| ||||| || |||||||| ||||||| |||||||| || || |||
Sbjct: 391 atggttcgcatattgtgcaatgattgcggggcggtatcagaggtgcagtttcacttaatt 450
Query: 271 gcgcacaagtgccagaatnnnnantcatacaacacccgcnagatttgaagt 321
|| || |||||||| | | | |||||||||||||| | |||||||||
Sbjct: 451 gcacataagtgccatagttgcaagtcatacaacacccgacaaatttgaagt 501
>gb|CD663353.1|CD663353 UCRHV18_05ah10_b1 Drought-stressed Dicktoo barley epidermis cDNA
library Hordeum vulgare subsp. vulgare cDNA clone
UCRHV18_05ah10, mRNA sequence
Length = 706
Score = 71.9 bits (36), Expect = 1e-011
Identities = 96/117 (82%)
Strand = Plus / Minus
Query: 15 gtacttgttcgattcgacaaatgatgtttccgtcctgccttnnngtcataccattcatgt 74
||||||||| || |||| ||||||||| || || ||||| | ||||||| |||||||
Sbjct: 559 gtacttgtttgagtcgagaaatgatgtctctgttctgccgtgtggtcatacaattcatga 500
Query: 75 aaagtgcctgaaagaaatggaagagcactgccagtttgcatgccccctttgctccaa 131
||| ||| ||||||||||| | ||||| ||||| || |||||||| || ||||||||
Sbjct: 499 aaaatgcttgaaagaaatgaaggagcattgccaattcgcatgcccgctctgctccaa 443
Score = 58.0 bits (29), Expect = 2e-007
Identities = 89/111 (80%)
Strand = Plus / Minus
Query: 211 atggtccgcatattatgcaacgactgcggggcaatatcagatgtgcagttccatttgatt 270
||||| |||||||| ||||| || |||||||| ||||||| |||||||| || || |||
Sbjct: 363 atggttcgcatattgtgcaatgattgcggggcggtatcagaggtgcagtttcacttaatt 304
Query: 271 gcgcacaagtgccagaatnnnnantcatacaacacccgcnagatttgaagt 321
|| || |||||||| | | | |||||||||||||| | |||||||||
Sbjct: 303 gcacataagtgccatagttgcaagtcatacaacacccggcaaatttgaagt 253
>gb|BU987196.1|BU987196 HF14B07r HF Hordeum vulgare subsp. vulgare cDNA clone HF14B07
5-PRIME, mRNA sequence
Length = 613
Score = 63.9 bits (32), Expect = 3e-009
Identities = 86/105 (81%)
Strand = Plus / Plus
Query: 15 gtacttgttcgattcgacaaatgatgtttccgtcctgccttnnngtcataccattcatgt 74
||||||||| || |||| ||||||||| || || ||||| | ||||||| |||||||
Sbjct: 508 gtacttgtttgagtcgagaaatgatgtctctgttctgccgtgtggtcatacaattcatga 567
Query: 75 aaagtgcctgaaagaaatggaagagcactgccagtttgcatgccc 119
||| ||| ||||||||||| | ||||| ||||| || ||||||||
Sbjct: 568 aaaatgcttgaaagaaatgaaggagcattgccaattcgcatgccc 612
>gb|BJ474523.1|BJ474523 BJ474523 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal14f22 3', mRNA sequence
Length = 389
Score = 60.0 bits (30), Expect = 5e-008
Identities = 89/111 (80%)
Strand = Plus / Minus
Query: 211 atggtccgcatattatgcaacgactgcggggcaatatcagatgtgcagttccatttgatt 270
||||| |||||||| ||||| || |||||||| ||||||| |||||||| || || |||
Sbjct: 274 atggttcgcatattgtgcaatgattgcggggcggtatcagaggtgcagtttcacttaatt 215
Query: 271 gcgcacaagtgccagaatnnnnantcatacaacacccgcnagatttgaagt 321
|| || |||||||| | | | |||||||||||||| | |||||||||
Sbjct: 214 gcacataagtgccatagttgcaagtcatacaacacccgncaaatttgaagt 164
>gb|AV928420.1|AV928420 AV928420 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basdg02 3', mRNA sequence
Length = 752
Score = 58.0 bits (29), Expect = 2e-007
Identities = 89/111 (80%)
Strand = Plus / Minus
Query: 211 atggtccgcatattatgcaacgactgcggggcaatatcagatgtgcagttccatttgatt 270
||||| |||||||| ||||| || |||||||| ||||||| |||||||| || || |||
Sbjct: 614 atggttcgcatattgtgcaatgattgcggggcggtatcagaggtgcagtttcacttaatt 555
Query: 271 gcgcacaagtgccagaatnnnnantcatacaacacccgcnagatttgaagt 321
|| || |||||||| | | | |||||||||||||| | |||||||||
Sbjct: 554 gcacataagtgccatagttgcaagtcatacaacacccggcaaatttgaagt 504
Score = 42.1 bits (21), Expect = 0.012
Identities = 42/49 (85%)
Strand = Plus / Minus
Query: 83 tgaaagaaatggaagagcactgccagtttgcatgccccctttgctccaa 131
||||||||||| | ||||| ||||| || |||||||| || ||||||||
Sbjct: 742 tgaaagaaatgaaggagcattgccaattcgcatgcccgctctgctccaa 694
>gb|BQ664207.1|BQ664207 HV02F10u HV Hordeum vulgare subsp. vulgare cDNA clone HV02F10
3-PRIME, mRNA sequence
Length = 497
Score = 58.0 bits (29), Expect = 2e-007
Identities = 89/111 (80%)
Strand = Plus / Minus
Query: 211 atggtccgcatattatgcaacgactgcggggcaatatcagatgtgcagttccatttgatt 270
||||| |||||||| ||||| || |||||||| ||||||| |||||||| || || |||
Sbjct: 348 atggttcgcatattgtgcaatgattgcggggcggtatcagaggtgcagtttcacttaatt 289
Query: 271 gcgcacaagtgccagaatnnnnantcatacaacacccgcnagatttgaagt 321
|| || |||||||| | | | |||||||||||||| | |||||||||
Sbjct: 288 gcacataagtgccatagttgcaagtcatacaacacccgacaaatttgaagt 238
Score = 52.0 bits (26), Expect = 1e-005
Identities = 59/70 (84%)
Strand = Plus / Minus
Query: 62 ataccattcatgtaaagtgcctgaaagaaatggaagagcactgccagtttgcatgccccc 121
|||| ||||||| ||| ||| ||||||||||| | ||||| ||||| || |||||||| |
Sbjct: 497 atacaattcatgaaaaatgcttgaaagaaatgaaggagcattgccaattcgcatgcccgc 438
Query: 122 tttgctccaa 131
| ||||||||
Sbjct: 437 tctgctccaa 428
>gb|BM097758.2|BM097758 EBem04_SQ002_J20_R embryo, 12 DPA, no treatment, cv Optic, EBem04
Hordeum vulgare subsp. vulgare cDNA clone
EBem04_SQ002_J20 5', mRNA sequence
Length = 424
Score = 58.0 bits (29), Expect = 2e-007
Identities = 89/111 (80%)
Strand = Plus / Plus
Query: 211 atggtccgcatattatgcaacgactgcggggcaatatcagatgtgcagttccatttgatt 270
||||| |||||||| ||||| || |||||||| ||||||| |||||||| || || |||
Sbjct: 80 atggttcgcatattgtgcaatgattgcggggcggtatcagaggtgcagtttcacttaatt 139
Query: 271 gcgcacaagtgccagaatnnnnantcatacaacacccgcnagatttgaagt 321
|| || |||||||| | | | |||||||||||||| | |||||||||
Sbjct: 140 gcacataagtgccatagttgcaagtcatacaacacccgacaaatttgaagt 190
>gb|CA010449.1|CA010449 HT09N07u HT Hordeum vulgare subsp. vulgare cDNA clone HT09N07
3-PRIME, mRNA sequence
Length = 488
Score = 58.0 bits (29), Expect = 2e-007
Identities = 89/111 (80%)
Strand = Plus / Minus
Query: 211 atggtccgcatattatgcaacgactgcggggcaatatcagatgtgcagttccatttgatt 270
||||| |||||||| ||||| || |||||||| ||||||| |||||||| || || |||
Sbjct: 344 atggttcgcatattgtgcaatgattgcggggcggtatcagaggtgcagtttcacttaatt 285
Query: 271 gcgcacaagtgccagaatnnnnantcatacaacacccgcnagatttgaagt 321
|| || |||||||| | | | |||||||||||||| | |||||||||
Sbjct: 284 gcacataagtgccatagttgcaagtcatacaacacccgacaaatttgaagt 234
Score = 50.1 bits (25), Expect = 5e-005
Identities = 55/65 (84%)
Strand = Plus / Minus
Query: 67 attcatgtaaagtgcctgaaagaaatggaagagcactgccagtttgcatgccccctttgc 126
||||||| ||| ||| ||||||||||| | ||||| ||||| || |||||||| || |||
Sbjct: 488 attcatgaaaaatgcttgaaagaaatgaaggagcattgccaattcgcatgcccgctctgc 429
Query: 127 tccaa 131
|||||
Sbjct: 428 tccaa 424
>gb|BU975768.1|BU975768 HB32D23r BC Hordeum vulgare subsp. vulgare cDNA clone HB32D23
5-PRIME, mRNA sequence
Length = 470
Score = 54.0 bits (27), Expect = 3e-006
Identities = 84/105 (80%)
Strand = Plus / Plus
Query: 217 cgcatattatgcaacgactgcggggcaatatcagatgtgcagttccatttgattgcgcac 276
|||||||| ||||| || |||||||| ||||||| |||||||| || || ||||| ||
Sbjct: 216 cgcatattgtgcaatgattgcggggcggtatcagaggtgcagtttcacttaattgcacat 275
Query: 277 aagtgccagaatnnnnantcatacaacacccgcnagatttgaagt 321
|||||||| | | | |||||||||||||| | |||||||||
Sbjct: 276 aagtgccatagttgcaagtcatacaacacccgacaaatttgaagt 320
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 15,228
Number of Sequences: 312970
Number of extensions: 15228
Number of successful extensions: 3989
Number of sequences better than 0.5: 13
Number of HSP's better than 0.5 without gapping: 10
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 3971
Number of HSP's gapped (non-prelim): 20
length of query: 344
length of database: 175,134,539
effective HSP length: 18
effective length of query: 326
effective length of database: 169,501,079
effective search space: 55257351754
effective search space used: 55257351754
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)