BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAH2a02.xg.2.1
(644 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ468196.1|BQ468196 HP01F17T HP Hordeum vulgare subsp. v... 192 1e-047
gb|CA024787.1|CA024787 HZ50E24r HZ Hordeum vulgare subsp. v... 66 2e-009
gb|BE060794.1|BE060794 HVSMEg0013G05f Hordeum vulgare pre-a... 64 6e-009
gb|CA031458.1|CA031458 HX10A06r HX Hordeum vulgare subsp. v... 54 6e-006
gb|CA015150.1|CA015150 HT13H01r HT Hordeum vulgare subsp. v... 48 4e-004
gb|BU999035.1|BU999035 HI12P13r HI Hordeum vulgare subsp. v... 46 0.001
gb|BF255897.2|BF255897 HVSMEf0008C05f Hordeum vulgare seedl... 44 0.006
gb|AW982533.3|AW982533 HVSMEg0003I09f Hordeum vulgare pre-a... 42 0.022
gb|BM817169.1|BM817169 HC109F04_T3.ab1 HC Hordeum vulgare s... 40 0.087
gb|AV836609.1|AV836609 AV836609 K. Sato unpublished cDNA li... 38 0.34
gb|BI958001.1|BI958001 HVSMEn0012N14f Hordeum vulgare rachi... 38 0.34
>gb|BQ468196.1|BQ468196 HP01F17T HP Hordeum vulgare subsp. vulgare cDNA clone HP01F17
5-PRIME, mRNA sequence
Length = 519
Score = 192 bits (97), Expect = 1e-047
Identities = 160/181 (88%)
Strand = Plus / Plus
Query: 462 gtcgcaagcacaacattgagcctcgcaatcctgacagctacttcaatcttaagaaggacc 521
||||||||||| ||| ||||||||||||||||| || ||||||| ||| |||| |||||
Sbjct: 11 gtcgcaagcacggcatcgagcctcgcaatcctgagagttacttcagtctgaagagggacc 70
Query: 522 catacaagaacaaggttcgccaggattttgtcaaggacaggaggagggtcaagagggagt 581
| |||||||||||||| ||| || ||||||||||||||||||||| ||||||||||||
Sbjct: 71 cctacaagaacaaggtacgctcagactttgtcaaggacaggaggaggatcaagagggagt 130
Query: 582 atgacgagttcaaggtcaggatcaatggtctgcctgactcgatacgccgacgctctgatg 641
||||||||||||||||||| || |||||| ||||||||||||| || || ||||||||||
Sbjct: 131 atgacgagttcaaggtcagaattaatggtttgcctgactcgatccgtcgccgctctgatg 190
Query: 642 c 642
|
Sbjct: 191 c 191
>gb|CA024787.1|CA024787 HZ50E24r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ50E24
5-PRIME, mRNA sequence
Length = 365
Score = 65.9 bits (33), Expect = 2e-009
Identities = 75/89 (84%)
Strand = Plus / Plus
Query: 305 cctccactggtcacagcaaacactattctgtccatccttgctgctgactaccctgtggag 364
||||| || ||||||||||||||||| || || ||||| |||| || || || || ||
Sbjct: 47 cctccgctagtcacagcaaacactatcctatctatcctggctgtagattatccggttgac 106
Query: 365 aagctttcttgctatgtttctgatgatgg 393
||||| |||||||||||||||||||||||
Sbjct: 107 aagctctcttgctatgtttctgatgatgg 135
>gb|BE060794.1|BE060794 HVSMEg0013G05f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0013G05f, mRNA sequence
Length = 624
Score = 63.9 bits (32), Expect = 6e-009
Identities = 68/80 (85%)
Strand = Plus / Plus
Query: 314 gtcacagcaaacactattctgtccatccttgctgctgactaccctgtggagaagctttct 373
||||||||||||||||| || || ||||| |||| || || || || || ||||| |||
Sbjct: 7 gtcacagcaaacactatcctatctatcctggctgtagattatccggttgacaagctctct 66
Query: 374 tgctatgtttctgatgatgg 393
||||||||||||||||||||
Sbjct: 67 tgctatgtttctgatgatgg 86
>gb|CA031458.1|CA031458 HX10A06r HX Hordeum vulgare subsp. vulgare cDNA clone HX10A06
5-PRIME, mRNA sequence
Length = 627
Score = 54.0 bits (27), Expect = 6e-006
Identities = 75/91 (82%)
Strand = Plus / Plus
Query: 305 cctccactggtcacagcaaacactattctgtccatccttgctgctgactaccctgtggag 364
||||| || ||||| ||||| ||| | || || |||||||||| ||||||||||| ||
Sbjct: 520 cctcctctcgtcactgcaaatactgtgctttctatccttgctgtggactaccctgttgac 579
Query: 365 aagctttcttgctatgtttctgatgatggag 395
|| | ||||||||||| |||||||||||||
Sbjct: 580 aaagtatcttgctatgtatctgatgatggag 610
>gb|CA015150.1|CA015150 HT13H01r HT Hordeum vulgare subsp. vulgare cDNA clone HT13H01
5-PRIME, mRNA sequence
Length = 526
Score = 48.1 bits (24), Expect = 4e-004
Identities = 54/64 (84%)
Strand = Plus / Plus
Query: 330 ttctgtccatccttgctgctgactaccctgtggagaagctttcttgctatgtttctgatg 389
|||||||||| ||||||| || |||||||| || || | || ||||||||||||||||
Sbjct: 4 ttctgtccattcttgctgtggattaccctgtcgacaaagtgtcatgctatgtttctgatg 63
Query: 390 atgg 393
||||
Sbjct: 64 atgg 67
>gb|BU999035.1|BU999035 HI12P13r HI Hordeum vulgare subsp. vulgare cDNA clone HI12P13
5-PRIME, mRNA sequence
Length = 656
Score = 46.1 bits (23), Expect = 0.001
Identities = 48/55 (87%), Gaps = 1/55 (1%)
Strand = Plus / Plus
Query: 341 cttgctgctgactaccctgtggagaagctttcttgctatgtttctgatgatggag 395
||||||| ||||||||||| || ||| | ||||||||||| |||||||||||||
Sbjct: 11 cttgctgtggactaccctgttgacaag-tatcttgctatgtatctgatgatggag 64
>gb|BF255897.2|BF255897 HVSMEf0008C05f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0008C05f, mRNA sequence
Length = 865
Score = 44.1 bits (22), Expect = 0.006
Identities = 28/30 (93%)
Strand = Plus / Minus
Query: 577 ggagtatgacgagttcaaggtcaggatcaa 606
|||||| ||||||||||||| |||||||||
Sbjct: 388 ggagtacgacgagttcaaggccaggatcaa 359
>gb|AW982533.3|AW982533 HVSMEg0003I09f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0003I09f, mRNA sequence
Length = 854
Score = 42.1 bits (21), Expect = 0.022
Identities = 51/61 (83%)
Strand = Plus / Plus
Query: 335 tccatccttgctgctgactaccctgtggagaagctttcttgctatgtttctgatgatgga 394
||||| ||||||| ||| || || ||||| ||| | ||||||||||| |||||||| |||
Sbjct: 81 tccattcttgctgttgattatcccgtggacaaggtctcttgctatgtatctgatgacgga 140
Query: 395 g 395
|
Sbjct: 141 g 141
>gb|BM817169.1|BM817169 HC109F04_T3.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC109F04_T3.ab1 similar to Xanthine
dehydrogenase,Hydrogen
dehydrogenase,Hydrogenase,Cytochrome-c3
hydrogenase,Retinal oxidase,Xanthine oxidase,Aldehyde
oxidase,Transferred entry: 1.18.99.1,, mRNA sequence
Length = 894
Score = 40.1 bits (20), Expect = 0.087
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 292 tccggagaaagaacctccac 311
||||||||||||||||||||
Sbjct: 142 tccggagaaagaacctccac 161
>gb|AV836609.1|AV836609 AV836609 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd18j24, mRNA
sequence
Length = 548
Score = 38.2 bits (19), Expect = 0.34
Identities = 37/43 (86%)
Strand = Plus / Plus
Query: 305 cctccactggtcacagcaaacactattctgtccatccttgctg 347
||||| || ||||||||||||||||| || || ||||| ||||
Sbjct: 500 cctccgctagtcacagcaaacactatcctatctatcctggctg 542
>gb|BI958001.1|BI958001 HVSMEn0012N14f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0012N14f, mRNA sequence
Length = 881
Score = 38.2 bits (19), Expect = 0.34
Identities = 31/35 (88%)
Strand = Plus / Plus
Query: 572 aagagggagtatgacgagttcaaggtcaggatcaa 606
||||||||||| || ||||||||||| |||||||
Sbjct: 187 aagagggagtacgaggagttcaaggtgcggatcaa 221
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 65,985
Number of Sequences: 312970
Number of extensions: 65985
Number of successful extensions: 17069
Number of sequences better than 0.5: 11
Number of HSP's better than 0.5 without gapping: 11
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 17051
Number of HSP's gapped (non-prelim): 17
length of query: 644
length of database: 175,134,539
effective HSP length: 19
effective length of query: 625
effective length of database: 169,188,109
effective search space: 105742568125
effective search space used: 105742568125
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)