BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF30a10.yg.2.1
(1130 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM101004.2|BM101004 EBpi01_SQ002_J03_R pistil, 1 DPA, no... 194 4e-048
gb|BQ760957.1|BQ760957 EBro03_SQ003_P10_R root, 3 week, wat... 184 4e-045
gb|BQ468445.1|BQ468445 HM01E10T HM Hordeum vulgare subsp. v... 46 0.003
gb|BQ468646.1|BQ468646 HM01N21T HM Hordeum vulgare subsp. v... 46 0.003
gb|BF266592.2|BF266592 HV_CEa0015I18f Hordeum vulgare seedl... 44 0.010
gb|CA032262.1|CA032262 HX12I03r HX Hordeum vulgare subsp. v... 42 0.039
gb|BF264341.2|BF264341 HV_CEa0009C16f Hordeum vulgare seedl... 40 0.16
>gb|BM101004.2|BM101004 EBpi01_SQ002_J03_R pistil, 1 DPA, no treatment, cv Optic, EBpi01
Hordeum vulgare subsp. vulgare cDNA clone
EBpi01_SQ002_J03 5', mRNA sequence
Length = 557
Score = 194 bits (98), Expect = 4e-048
Identities = 194/226 (85%)
Strand = Plus / Plus
Query: 601 ttgcaagaggcttagcttatctccacgaagagtctagcatccgcattgtgcatagggaca 660
||||||||||| | | ||||| || || || ||||||||||| ||||||| |||||||
Sbjct: 5 ttgcaagaggcctttcctatcttcatgaggaatctagcatccgtgttgtgcacagggaca 64
Query: 661 taaaggccagcaatgtattacttgatgccactttgaaccctaagatttcggatttcgggc 720
|||||||||||||| | ||||||||||||| | | || |||||||| ||||| || ||||
Sbjct: 65 taaaggccagcaatatcttacttgatgccaatcttaatcctaagatctcggactttgggc 124
Query: 721 tcgccaaactttatgacgataaaaagacacatgttagcacaaaagttgctggtacatttg 780
|||||||||||||||| || | ||||||||||| ||||| || ||||||||||||||||
Sbjct: 125 tcgccaaactttatgatgaccagaagacacatgtgagcacgaaggttgctggtacatttg 184
Query: 781 gctatctttcacctgagtacgccatgagaggtcacatgactgagaa 826
||||||| |||||||||| ||||||||||| || |||||||||||
Sbjct: 185 gctatctcgcacctgagtatgccatgagaggccatatgactgagaa 230
>gb|BQ760957.1|BQ760957 EBro03_SQ003_P10_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ003_P10 5', mRNA sequence
Length = 592
Score = 184 bits (93), Expect = 4e-045
Identities = 159/181 (87%)
Strand = Plus / Plus
Query: 646 ttgtgcatagggacataaaggccagcaatgtattacttgatgccactttgaaccctaaga 705
||||||| ||||||||||||||||||||| | ||||||||||||| | | || |||||||
Sbjct: 287 ttgtgcacagggacataaaggccagcaatatcttacttgatgccaatcttaatcctaaga 346
Query: 706 tttcggatttcgggctcgccaaactttatgacgataaaaagacacatgttagcacaaaag 765
| ||||| || |||||||||||||||||||| || | ||||||||||| ||||| ||||
Sbjct: 347 tctcggactttgggctcgccaaactttatgatgaccagaagacacatgtgagcaccaaag 406
Query: 766 ttgctggtacatttggctatctttcacctgagtacgccatgagaggtcacatgactgaga 825
| |||||||||||||||||||| |||||||||| ||||||||||| || ||||||||||
Sbjct: 407 tggctggtacatttggctatctcgcacctgagtatgccatgagaggccatatgactgaga 466
Query: 826 a 826
|
Sbjct: 467 a 467
Score = 105 bits (53), Expect = 3e-021
Identities = 98/113 (86%)
Strand = Plus / Plus
Query: 417 gaaatagaaactatttcccgagtgcagcaccgtaatcttgtgaagttgtttggtttttgc 476
|||||||||||||| || |||||||| ||||||||||| |||||||||| ||| | |||
Sbjct: 58 gaaatagaaactatatctcgagtgcaacaccgtaatctcgtgaagttgtatggctgctgc 117
Query: 477 cttgagggaaataaaccacttttggtttatgagtacatggagagtggaagcct 529
|||||||| ||||| ||||| | |||||||||||| |||||| |||||||||
Sbjct: 118 cttgagggcaataagccactgcttgtttatgagtacctggagaatggaagcct 170
>gb|BQ468445.1|BQ468445 HM01E10T HM Hordeum vulgare subsp. vulgare cDNA clone HM01E10
5-PRIME, mRNA sequence
Length = 588
Score = 46.1 bits (23), Expect = 0.003
Identities = 23/23 (100%)
Strand = Plus / Plus
Query: 804 atgagaggtcacatgactgagaa 826
|||||||||||||||||||||||
Sbjct: 11 atgagaggtcacatgactgagaa 33
>gb|BQ468646.1|BQ468646 HM01N21T HM Hordeum vulgare subsp. vulgare cDNA clone HM01N21
5-PRIME, mRNA sequence
Length = 546
Score = 46.1 bits (23), Expect = 0.003
Identities = 23/23 (100%)
Strand = Plus / Plus
Query: 804 atgagaggtcacatgactgagaa 826
|||||||||||||||||||||||
Sbjct: 11 atgagaggtcacatgactgagaa 33
>gb|BF266592.2|BF266592 HV_CEa0015I18f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0015I18f, mRNA sequence
Length = 520
Score = 44.1 bits (22), Expect = 0.010
Identities = 25/26 (96%)
Strand = Plus / Plus
Query: 651 catagggacataaaggccagcaatgt 676
||||||||||| ||||||||||||||
Sbjct: 62 catagggacatcaaggccagcaatgt 87
>gb|CA032262.1|CA032262 HX12I03r HX Hordeum vulgare subsp. vulgare cDNA clone HX12I03
5-PRIME, mRNA sequence
Length = 587
Score = 42.1 bits (21), Expect = 0.039
Identities = 27/29 (93%)
Strand = Plus / Plus
Query: 504 tatgagtacatggagagtggaagccttga 532
|||||||||||||||| ||| ||||||||
Sbjct: 341 tatgagtacatggagaatggcagccttga 369
>gb|BF264341.2|BF264341 HV_CEa0009C16f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0009C16f, mRNA sequence
Length = 829
Score = 40.1 bits (20), Expect = 0.16
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 711 gatttcgggctcgccaaact 730
||||||||||||||||||||
Sbjct: 139 gatttcgggctcgccaaact 158
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 69,540
Number of Sequences: 312970
Number of extensions: 69540
Number of successful extensions: 16716
Number of sequences better than 0.5: 7
Number of HSP's better than 0.5 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 16707
Number of HSP's gapped (non-prelim): 9
length of query: 1130
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1111
effective length of database: 169,188,109
effective search space: 187967989099
effective search space used: 187967989099
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)