BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF18e02.yg.3.1
(315 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU989082.1|BU989082 HF19L09r HF Hordeum vulgare subsp. v... 94 3e-018
gb|BE437521.1|BE437521 SFR004.F04F990621 ITEC SFR Barley Le... 92 1e-017
gb|BU974965.1|BU974965 HB29K23r BC Hordeum vulgare subsp. v... 52 1e-005
gb|AV836624.1|AV836624 AV836624 K. Sato unpublished cDNA li... 42 0.011
gb|AV833855.1|AV833855 AV833855 K. Sato unpublished cDNA li... 36 0.65
gb|BI951055.1|BI951055 HVSMEl0024C19f Hordeum vulgare spike... 36 0.65
gb|BU990095.1|BU990095 HF24B04r HF Hordeum vulgare subsp. v... 36 0.65
gb|BI947086.1|BI947086 HVSMEl0003J17f Hordeum vulgare spike... 34 2.6
>gb|BU989082.1|BU989082 HF19L09r HF Hordeum vulgare subsp. vulgare cDNA clone HF19L09
5-PRIME, mRNA sequence
Length = 614
Score = 93.7 bits (47), Expect = 3e-018
Identities = 207/260 (79%), Gaps = 8/260 (3%)
Strand = Plus / Plus
Query: 60 catatttcatatttttccattgattcatatgcaaagctcgtggttatggttctcaagtac 119
||||| |||||||| |||||||||||||| | ||||| ||| | ||| || ||| ||
Sbjct: 157 catatatcatatttccccattgattcatattcgaagcttgtgtctgcggtgctaaagaac 216
Query: 120 tcttcagtggagataacaccaaataaaggcagtatactttccaagattatttctgttact 179
| ||||||||| ||| |||||| |||||||| | ||| |||||||| ||||||||
Sbjct: 217 tgttcagtggaaataggaccaaacaaaggcagccttcttcccaagatt----ctgttact 272
Query: 180 g----tgaggaccattcagaaagatgctgaggaaaagaaagcttcatttaatccccgacc 235
| | |||| ||| |||||||| ||||| || ||||||||||||||||| ||||||||
Sbjct: 273 gacaattaggatcatccagaaagaagctgaagagaagaaagcttcatttaacccccgacc 332
Query: 236 atacttccgattgtntataaattggctctatgaccttactactactgatggccatcatga 295
|||||| || ||| |||||||||||| ||||||||| ||| |||| ||||||||
Sbjct: 333 atactttcgtctgtttataaattggctgaatgaccttattacatctgaacctcatcatga 392
Query: 296 tggttctaattttcaggttt 315
||| ||||||| |||||||
Sbjct: 393 tggaactaatttgcaggttt 412
Score = 42.1 bits (21), Expect = 0.011
Identities = 30/33 (90%)
Strand = Plus / Plus
Query: 1 tagtctccgagcaaattgttgctcctggtggat 33
||||||| ||||||||| ||||||||||||||
Sbjct: 107 tagtctctgagcaaattactgctcctggtggat 139
>gb|BE437521.1|BE437521 SFR004.F04F990621 ITEC SFR Barley Leaf Epidermis Library Hordeum
vulgare subsp. vulgare cDNA clone SF, mRNA sequence
Length = 839
Score = 91.7 bits (46), Expect = 1e-017
Identities = 111/133 (83%)
Strand = Plus / Plus
Query: 183 aggaccattcagaaagatgctgaggaaaagaaagcttcatttaatccccgaccatacttc 242
|||| ||| |||||||| ||||| || ||||||||||||||||| ||||||||||||||
Sbjct: 77 aggatcatccagaaagaagctgaagagaagaaagcttcatttaacccccgaccatacttt 136
Query: 243 cgattgtntataaattggctctatgaccttactactactgatggccatcatgatggttct 302
|| ||| |||||||||||| ||||||||| ||| |||| ||||||||||| ||
Sbjct: 137 cgtctgtttataaattggctgaatgaccttattacatctgaacctcatcatgatggaact 196
Query: 303 aattttcaggttt 315
||||| |||||||
Sbjct: 197 aatttgcaggttt 209
>gb|BU974965.1|BU974965 HB29K23r BC Hordeum vulgare subsp. vulgare cDNA clone HB29K23
5-PRIME, mRNA sequence
Length = 611
Score = 52.0 bits (26), Expect = 1e-005
Identities = 32/34 (94%)
Strand = Plus / Plus
Query: 183 aggaccattcagaaagatgctgaggaaaagaaag 216
|||| |||||||||||||||||| ||||||||||
Sbjct: 180 aggatcattcagaaagatgctgaagaaaagaaag 213
>gb|AV836624.1|AV836624 AV836624 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd19c09, mRNA
sequence
Length = 620
Score = 42.1 bits (21), Expect = 0.011
Identities = 30/33 (90%)
Strand = Plus / Plus
Query: 1 tagtctccgagcaaattgttgctcctggtggat 33
||||||| ||||||||| ||||||||||||||
Sbjct: 520 tagtctctgagcaaattactgctcctggtggat 552
Score = 36.2 bits (18), Expect = 0.65
Identities = 27/30 (90%)
Strand = Plus / Plus
Query: 60 catatttcatatttttccattgattcatat 89
||||| |||||||| ||||||||||||||
Sbjct: 570 catatatcatatttccccattgattcatat 599
>gb|AV833855.1|AV833855 AV833855 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags18b14, mRNA sequence
Length = 640
Score = 36.2 bits (18), Expect = 0.65
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 200 tgctgaggaaaagaaagc 217
||||||||||||||||||
Sbjct: 79 tgctgaggaaaagaaagc 96
>gb|BI951055.1|BI951055 HVSMEl0024C19f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0024C19f, mRNA sequence
Length = 854
Score = 36.2 bits (18), Expect = 0.65
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 41 gcaatcaccacagcaacc 58
||||||||||||||||||
Sbjct: 62 gcaatcaccacagcaacc 79
>gb|BU990095.1|BU990095 HF24B04r HF Hordeum vulgare subsp. vulgare cDNA clone HF24B04
5-PRIME, mRNA sequence
Length = 505
Score = 36.2 bits (18), Expect = 0.65
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 200 tgctgaggaaaagaaagc 217
||||||||||||||||||
Sbjct: 43 tgctgaggaaaagaaagc 60
>gb|BI947086.1|BI947086 HVSMEl0003J17f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0003J17f, mRNA sequence
Length = 1046
Score = 34.2 bits (17), Expect = 2.6
Identities = 17/17 (100%)
Strand = Plus / Minus
Query: 217 cttcatttaatccccga 233
|||||||||||||||||
Sbjct: 685 cttcatttaatccccga 669
Database: Hordeum_nucl_with_EST.fasta
Posted date: May 2, 2006 2:38 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 28,622
Number of Sequences: 312970
Number of extensions: 28622
Number of successful extensions: 9150
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 9134
Number of HSP's gapped (non-prelim): 14
length of query: 315
length of database: 175,134,539
effective HSP length: 18
effective length of query: 297
effective length of database: 169,501,079
effective search space: 50341820463
effective search space used: 50341820463
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)