BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAE36e08.yg.2.1
(352 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF257531.2|BF257531 HVSMEf0013C16f Hordeum vulgare seedl... 379 e-104
gb|BM370198.2|BM370198 EBro08_SQ003_E17_R root, 3 week, dro... 345 6e-094
gb|BM816903.1|BM816903 HC113F11_SK.ab1 HC Hordeum vulgare s... 281 7e-075
gb|BE519775.3|BE519775 HV_CEb0021C20f Hordeum vulgare seedl... 64 3e-009
gb|BI956973.1|BI956973 HVSMEn0006I16f Hordeum vulgare rachi... 60 5e-008
gb|BM375016.1|BM375016 EBem06_SQ002_B13_R embryo, 21 DPA, n... 58 2e-007
gb|BE214373.1|BE214373 HV_CEb0003E06f Hordeum vulgare seedl... 56 8e-007
gb|BI958400.1|BI958400 HVSMEn0014M17f Hordeum vulgare rachi... 44 0.003
gb|BE421314.1|BE421314 HWM008.A07 ITEC HWM Barley Leaf Libr... 42 0.012
emb|AJ234679.1|HVU234679 Hordeum vulgare genomic DNA fragme... 40 0.047
>gb|BF257531.2|BF257531 HVSMEf0013C16f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0013C16f, mRNA sequence
Length = 495
Score = 379 bits (191), Expect = e-104
Identities = 286/318 (89%), Gaps = 1/318 (0%)
Strand = Plus / Minus
Query: 3 gggtcgtagttgccgaaccccgtggccagcacgtagaagtggtagccgtggatgtgcatg 62
|||| |||||||||||| || ||||| | |||||||||||||||||||||||||||||||
Sbjct: 342 gggttgtagttgccgaagcctgtggcgaacacgtagaagtggtagccgtggatgtgcatg 283
Query: 63 gggtgctcctcggcggcgaa-gatgcccgtgtcctggaacacgatctgcaccacggcgcc 121
||||||||||| | ||||| ||||||||||||||||||||| |||||||||||||||||
Sbjct: 282 gggtgctcctcccccgcgaaagatgcccgtgtcctggaacacaatctgcaccacggcgcc 223
Query: 122 gtacctgacgcggtacaggcgggtgcccttgacgggctgccacagcgcgcgcgggacgtt 181
| || ||||||||||||| || || ||||||||||||||||| |||||||| ||||||||
Sbjct: 222 ggacttgacgcggtacagccgcgtccccttgacgggctgccagagcgcgcgggggacgtt 163
Query: 182 ctgcgaggtgaagtcgaagaacacgggcgggagcgccgggaagtcgtcggtgaacacgcc 241
|||||||||| ||||||||| ||| |||||| | || |||||||| |||||||| |||||
Sbjct: 162 ctgcgaggtgtagtcgaagaccaccggcgggcgagcggggaagtcctcggtgaagacgcc 103
Query: 242 ggggatgtggtggtagtgcgcctgcagcagggagacggtgtcggggagctggaacgacac 301
||||||| ||||||||||||||||||||||||||||||||| ||||||||||||||||||
Sbjct: 102 ggggatgcggtggtagtgcgcctgcagcagggagacggtgttggggagctggaacgacac 43
Query: 302 gnnnnncatgctcgcgcc 319
| ||||||||||||
Sbjct: 42 gttgttcatgctcgcgcc 25
>gb|BM370198.2|BM370198 EBro08_SQ003_E17_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ003_E17 5', mRNA sequence
Length = 638
Score = 345 bits (174), Expect = 6e-094
Identities = 241/265 (90%)
Strand = Plus / Minus
Query: 55 tgtgcatggggtgctcctcggcggcgaagatgcccgtgtcctggaacacgatctgcacca 114
||||||||||||||||||| || |||||||||||||||||||||||||| ||||||||||
Sbjct: 638 tgtgcatggggtgctcctccgccgcgaagatgcccgtgtcctggaacacaatctgcacca 579
Query: 115 cggcgccgtacctgacgcggtacaggcgggtgcccttgacgggctgccacagcgcgcgcg 174
||||||||||| ||||||||||||| || || ||||||||||||||||| |||||||| |
Sbjct: 578 cggcgccgtacttgacgcggtacagccgcgtccccttgacgggctgccagagcgcgcggg 519
Query: 175 ggacgttctgcgaggtgaagtcgaagaacacgggcgggagcgccgggaagtcgtcggtga 234
||||||||||||||||| ||||||||| ||| |||||| | || |||||||| |||||||
Sbjct: 518 ggacgttctgcgaggtgtagtcgaagaccaccggcgggcgagcggggaagtcctcggtga 459
Query: 235 acacgccggggatgtggtggtagtgcgcctgcagcagggagacggtgtcggggagctgga 294
| |||||||||||| ||||||||||||||||||||||||||||||||| |||||||||||
Sbjct: 458 agacgccggggatgcggtggtagtgcgcctgcagcagggagacggtgttggggagctgga 399
Query: 295 acgacacgnnnnncatgctcgcgcc 319
|||||||| ||||||||||||
Sbjct: 398 acgacacgttgttcatgctcgcgcc 374
>gb|BM816903.1|BM816903 HC113F11_SK.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC113F11_SK.ab1 similar to Laccase,Catechol
oxidase,L-ascorbate oxidase,, mRNA sequence
Length = 883
Score = 281 bits (142), Expect = 7e-075
Identities = 269/304 (88%), Gaps = 8/304 (2%)
Strand = Plus / Minus
Query: 24 gtggccagcacgtagaagtgg-tagccgtggatgtgcatggggtgctcctcg-gcggcga 81
||||| |||||||| |||||| |||||||| |||||||||||||||||||| || ||||
Sbjct: 841 gtggcgagcacgtacaagtgggtagccgtgcatgtgcatggggtgctcctcccgccgcga 782
Query: 82 agatgcccgtgtcctggaa-cacgatctgc-accacgg-cgccgtacctgacgcggtaca 138
||||||||||||||||||| ||| |||||| ||||||| |||||||| ||||||||||||
Sbjct: 781 agatgcccgtgtcctggaaacacaatctgccaccacgggcgccgtacttgacgcggtaca 722
Query: 139 ggcgggtgccc--ttgacgggctgccacagcgcgcgcgggacgttctgcgaggtgaagtc 196
| || || ||| |||||||||||||| |||||||| |||||||||||||||||| ||||
Sbjct: 721 gccgcgtccccccttgacgggctgccagagcgcgcgggggacgttctgcgaggtgtagtc 662
Query: 197 gaagaacacg-ggcgggagcgccgggaagtcgtcggtgaacacgccggggatgtggtggt 255
||||| ||| |||||| | || |||||||| |||||||| |||||||||||| ||||||
Sbjct: 661 gaagaccacccggcgggcgagcggggaagtcctcggtgaagacgccggggatgcggtggt 602
Query: 256 agtgcgcctgcagcagggagacggtgtcggggagctggaacgacacgnnnnncatgctcg 315
||||||||||||||||||||||||||| ||||||||||||||||||| ||||||||
Sbjct: 601 agtgcgcctgcagcagggagacggtgttggggagctggaacgacacgttgttcatgctcg 542
Query: 316 cgcc 319
||||
Sbjct: 541 cgcc 538
>gb|BE519775.3|BE519775 HV_CEb0021C20f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0021C20f, mRNA sequence
Length = 699
Score = 63.9 bits (32), Expect = 3e-009
Identities = 56/64 (87%)
Strand = Plus / Minus
Query: 3 gggtcgtagttgccgaaccccgtggccagcacgtagaagtggtagccgtggatgtgcatg 62
|||| ||||||||||| ||||||||| || ||| |||||| |||||| |||| |||||||
Sbjct: 698 gggttgtagttgccgagccccgtggcgaggacgaagaagtcgtagccatggaggtgcatg 639
Query: 63 gggt 66
||||
Sbjct: 638 gggt 635
>gb|BI956973.1|BI956973 HVSMEn0006I16f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0006I16f, mRNA sequence
Length = 834
Score = 60.0 bits (30), Expect = 5e-008
Identities = 69/82 (84%)
Strand = Plus / Minus
Query: 44 gtagccgtggatgtgcatggggtgctcctcggcggcgaagatgcccgtgtcctggaacac 103
|||||| |||| |||||| ||||| | ||| ||||||||||| ||| |||||| |||
Sbjct: 621 gtagccatggaggtgcatagggtggttctccccggcgaagatgttggtgccctggagcac 562
Query: 104 gatctgcaccacggcgccgtac 125
|||||||||||||| |||||||
Sbjct: 561 gatctgcaccacggagccgtac 540
>gb|BM375016.1|BM375016 EBem06_SQ002_B13_R embryo, 21 DPA, no treatment, cv Optic, EBem06
Hordeum vulgare subsp. vulgare cDNA clone
EBem06_SQ002_B13 5', mRNA sequence
Length = 389
Score = 58.0 bits (29), Expect = 2e-007
Identities = 74/89 (83%)
Strand = Plus / Plus
Query: 37 agaagtggtagccgtggatgtgcatggggtgctcctcggcggcgaagatgcccgtgtcct 96
|||||| |||||| |||| ||| || ||||| | ||| ||||||||||| ||| |||
Sbjct: 201 agaagtcgtagccatggaggtggatagggtggttctccccggcgaagatgttggtgccct 260
Query: 97 ggaacacgatctgcaccacggcgccgtac 125
||| ||||||||||||||||| |||||||
Sbjct: 261 ggagcacgatctgcaccacggagccgtac 289
>gb|BE214373.1|BE214373 HV_CEb0003E06f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0003E06f, mRNA sequence
Length = 709
Score = 56.0 bits (28), Expect = 8e-007
Identities = 55/64 (85%)
Strand = Plus / Plus
Query: 3 gggtcgtagttgccgaaccccgtggccagcacgtagaagtggtagccgtggatgtgcatg 62
|||| ||||||||||| ||||||||| || ||| |||| | |||||| |||| |||||||
Sbjct: 548 gggttgtagttgccgagccccgtggcgaggacgaagaaatcgtagccatggaggtgcatg 607
Query: 63 gggt 66
||||
Sbjct: 608 gggt 611
>gb|BI958400.1|BI958400 HVSMEn0014M17f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0014M17f, mRNA sequence
Length = 677
Score = 44.1 bits (22), Expect = 0.003
Identities = 57/66 (86%), Gaps = 2/66 (3%)
Strand = Plus / Minus
Query: 3 gggtcgtagttgccgaaccccgtggcc-agcacgtagaagtggtagccgtggatgtgcat 61
||||||||||||||||| ||| ||||| | |||| |||||| | ||||||| |||||||
Sbjct: 540 gggtcgtagttgccgaa-cccttggcctaccacgaagaagttgaagccgtgcatgtgcag 482
Query: 62 ggggtg 67
||||||
Sbjct: 481 ggggtg 476
>gb|BE421314.1|BE421314 HWM008.A07 ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM008.A07, mRNA sequence
Length = 905
Score = 42.1 bits (21), Expect = 0.012
Identities = 99/125 (79%)
Strand = Plus / Minus
Query: 5 gtcgtagttgccgaaccccgtggccagcacgtagaagtggtagccgtggatgtgcatggg 64
||||||||||||||| ||| ||| || | |||||||| ||| ||||||| ||| |||||
Sbjct: 448 gtcgtagttgccgaagccctcggcgaggatgtagaagtcgtatccgtggaggtggatggg 389
Query: 65 gtgctcctcggcggcgaagatgcccgtgtcctggaacacgatctgcaccacggcgccgta 124
|| | ||| | | || ||||| |||||||| | |||| ||||||||||| |||| |
Sbjct: 388 atggttctccggcgtgacgatgctggtgtcctgcaggacgacctgcaccacggagccgaa 329
Query: 125 cctga 129
|||||
Sbjct: 328 cctga 324
Score = 40.1 bits (20), Expect = 0.047
Identities = 80/100 (80%)
Strand = Plus / Minus
Query: 176 gacgttctgcgaggtgaagtcgaagaacacgggcgggagcgccgggaagtcgtcggtgaa 235
||||||||||| ||| ||||||| ||| |||||| ||||||||||| ||||||
Sbjct: 277 gacgttctgcgccgtgtagtcgaactgcaccggcgggtaggccgggaagtcagtggtgaa 218
Query: 236 cacgccggggatgtggtggtagtgcgcctgcagcagggag 275
|||||| || |||| || ||||||||| || || ||||||
Sbjct: 217 cacgcccggcatgtcgtagtagtgcgcgtggaggagggag 178
>emb|AJ234679.1|HVU234679 Hordeum vulgare genomic DNA fragment; clone MWG0961.uni
Length = 510
Score = 40.1 bits (20), Expect = 0.047
Identities = 80/100 (80%)
Strand = Plus / Plus
Query: 176 gacgttctgcgaggtgaagtcgaagaacacgggcgggagcgccgggaagtcgtcggtgaa 235
||||||||||| ||| ||||||| ||| |||||| ||||||||||| ||||||
Sbjct: 76 gacgttctgcgccgtgtagtcgaactgcaccggcgggtaggccgggaagtcagtggtgaa 135
Query: 236 cacgccggggatgtggtggtagtgcgcctgcagcagggag 275
|||||| || |||| || ||||||||| || || ||||||
Sbjct: 136 cacgcccggcatgtcgtagtagtgcgcgtggaggagggag 175
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 45,983
Number of Sequences: 312970
Number of extensions: 45983
Number of successful extensions: 15137
Number of sequences better than 0.5: 11
Number of HSP's better than 0.5 without gapping: 11
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 15112
Number of HSP's gapped (non-prelim): 19
length of query: 352
length of database: 175,134,539
effective HSP length: 18
effective length of query: 334
effective length of database: 169,501,079
effective search space: 56613360386
effective search space used: 56613360386
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)