BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 8636621.2.1
(691 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AL500488.1|AL500488 AL500488 Hordeum vulgare Barke etiol... 228 2e-058
gb|AV923752.1|AV923752 AV923752 K. Sato unpublished cDNA li... 111 3e-023
gb|AV927277.1|AV927277 AV927277 K. Sato unpublished cDNA li... 111 3e-023
gb|BF621150.3|BF621150 HVSMEa0004J06f Hordeum vulgare seedl... 58 4e-007
gb|BI957153.1|BI957153 HVSMEn0007M22f Hordeum vulgare rachi... 42 0.024
gb|CA030254.1|CA030254 HX06I12r HX Hordeum vulgare subsp. v... 42 0.024
gb|CB874858.1|CB874858 HX06I12w HX Hordeum vulgare subsp. v... 42 0.024
gb|BE060220.1|BE060220 HVSMEg0011K01f Hordeum vulgare pre-a... 38 0.37
gb|BE216796.2|BE216796 HV_CEb0011J19f Hordeum vulgare seedl... 38 0.37
>gb|AL500488.1|AL500488 AL500488 Hordeum vulgare Barke etiolated leaves Hordeum vulgare
subsp. vulgare cDNA clone HK06N15r 3', mRNA sequence
Length = 339
Score = 228 bits (115), Expect = 2e-058
Identities = 227/265 (85%)
Strand = Plus / Plus
Query: 372 cttcttctcggcgctcttgccccacagcacgaagtagaggccgatgacgatcagcacagc 431
||||||||| ||||||| ||||||||||||||||| || ||||| ||||||||||| ||
Sbjct: 72 cttcttctcctcgctcttcccccacagcacgaagtatagcccgattacgatcagcaccgc 131
Query: 432 tccaatgatccctcccgtgtagagctggtccccgaggatggcggaagccatgacggcgac 491
|| ||||||||||| ||||| |||||||| ||||||||| ||| ||||||||||||||
Sbjct: 132 cccgatgatccctccggtgtanagctggtcgccgaggatgacggcggccatgacggcgac 191
Query: 492 ggccacggtctgcactggctggaagacggcggtgaagagcgggcctcccctgtcgatgca 551
||| |||||||| || ||||||||||||||||||||||| ||||| || | |||||||||
Sbjct: 192 ggcgacggtctggacgggctggaagacggcggtgaagagagggccgccgcggtcgatgca 251
Query: 552 ccagatctgcagagcaaacgctaccccggatgccacaaggccagcgtataggatggtgaa 611
||| ||||| || || || || || ||||| || || || || ||||| |||||||||||
Sbjct: 252 ccanatctggagcgcgaaggcaacgccggaggcaaccagcccggcgtaaaggatggtgaa 311
Query: 612 gagctcccctcctgagcggaccttc 636
||||||||| || || |||||||||
Sbjct: 312 gagctccccgccggaccggaccttc 336
>gb|AV923752.1|AV923752 AV923752 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd1f03 5', mRNA sequence
Length = 525
Score = 111 bits (56), Expect = 3e-023
Identities = 89/100 (89%)
Strand = Plus / Minus
Query: 372 cttcttctcggcgctcttgccccacagcacgaagtagaggccgatgacgatcagcacagc 431
||||||||| ||||||| ||||||||||||||||| || ||||| ||||||||||| ||
Sbjct: 398 cttcttctcctcgctcttcccccacagcacgaagtatagcccgattacgatcagcaccgc 339
Query: 432 tccaatgatccctcccgtgtagagctggtccccgaggatg 471
|| ||||||||||| |||||||||||||| |||||||||
Sbjct: 338 cccgatgatccctccggtgtagagctggtcgccgaggatg 299
>gb|AV927277.1|AV927277 AV927277 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd1f03 3', mRNA sequence
Length = 647
Score = 111 bits (56), Expect = 3e-023
Identities = 89/100 (89%)
Strand = Plus / Plus
Query: 372 cttcttctcggcgctcttgccccacagcacgaagtagaggccgatgacgatcagcacagc 431
||||||||| ||||||| ||||||||||||||||| || ||||| ||||||||||| ||
Sbjct: 312 cttcttctcctcgctcttcccccacagcacgaagtatagcccgattacgatcagcaccgc 371
Query: 432 tccaatgatccctcccgtgtagagctggtccccgaggatg 471
|| ||||||||||| |||||||||||||| |||||||||
Sbjct: 372 cccgatgatccctccggtgtagagctggtcgccgaggatg 411
>gb|BF621150.3|BF621150 HVSMEa0004J06f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0004J06f, mRNA sequence
Length = 735
Score = 58.0 bits (29), Expect = 4e-007
Identities = 74/89 (83%)
Strand = Plus / Minus
Query: 603 gatggtgaagagctcccctcctgagcggaccttccacctgctcaggtcctcctcggtgaa 662
|||||| |||||||||| || || |||||||||||| | | |||||| ||||||||||
Sbjct: 602 gatggttaagagctcccagccggaccggaccttccacaggatgaggtccacctcggtgaa 543
Query: 663 ggcggcaatggccaggaactggaggagcc 691
||||| ||| | ||||||||||| ||||
Sbjct: 542 ggcggagatgacgaggaactggagcagcc 514
>gb|BI957153.1|BI957153 HVSMEn0007M22f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0007M22f, mRNA sequence
Length = 1133
Score = 42.1 bits (21), Expect = 0.024
Identities = 51/61 (83%)
Strand = Plus / Minus
Query: 383 cgctcttgccccacagcacgaagtagaggccgatgacgatcagcacagctccaatgatcc 442
||||||||||||| ||||||| |||||| ||| ||| ||| ||| | || || |||||||
Sbjct: 522 cgctcttgccccagagcacgaggtagagcccggtgatgatgagcgcggcgccgatgatcc 463
Query: 443 c 443
|
Sbjct: 462 c 462
Score = 38.2 bits (19), Expect = 0.37
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 544 tcgatgcaccagatctgca 562
|||||||||||||||||||
Sbjct: 361 tcgatgcaccagatctgca 343
>gb|CA030254.1|CA030254 HX06I12r HX Hordeum vulgare subsp. vulgare cDNA clone HX06I12
5-PRIME, mRNA sequence
Length = 651
Score = 42.1 bits (21), Expect = 0.024
Identities = 51/61 (83%)
Strand = Plus / Minus
Query: 383 cgctcttgccccacagcacgaagtagaggccgatgacgatcagcacagctccaatgatcc 442
||||||||||||| ||||||| |||||| ||| ||| ||| ||| | || || |||||||
Sbjct: 643 cgctcttgccccagagcacgaggtagagcccggtgatgatgagcgcggcgccgatgatcc 584
Query: 443 c 443
|
Sbjct: 583 c 583
>gb|CB874858.1|CB874858 HX06I12w HX Hordeum vulgare subsp. vulgare cDNA clone HX06I12
3-PRIME, mRNA sequence
Length = 564
Score = 42.1 bits (21), Expect = 0.024
Identities = 51/61 (83%)
Strand = Plus / Plus
Query: 383 cgctcttgccccacagcacgaagtagaggccgatgacgatcagcacagctccaatgatcc 442
||||||||||||| ||||||| |||||| ||| ||| ||| ||| | || || |||||||
Sbjct: 349 cgctcttgccccagagcacgaggtagagcccggtgatgatgagcgcggcgccgatgatcc 408
Query: 443 c 443
|
Sbjct: 409 c 409
>gb|BE060220.1|BE060220 HVSMEg0011K01f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0011K01f, mRNA sequence
Length = 645
Score = 38.2 bits (19), Expect = 0.37
Identities = 25/27 (92%)
Strand = Plus / Minus
Query: 384 gctcttgccccacagcacgaagtagag 410
|||||||||||| ||||||| ||||||
Sbjct: 472 gctcttgccccagagcacgaggtagag 446
Score = 38.2 bits (19), Expect = 0.37
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 544 tcgatgcaccagatctgca 562
|||||||||||||||||||
Sbjct: 311 tcgatgcaccagatctgca 293
>gb|BE216796.2|BE216796 HV_CEb0011J19f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0011J19f, mRNA sequence
Length = 825
Score = 38.2 bits (19), Expect = 0.37
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 644 tcaggtcctcctcggtgaa 662
|||||||||||||||||||
Sbjct: 193 tcaggtcctcctcggtgaa 175
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 83,188
Number of Sequences: 312970
Number of extensions: 83188
Number of successful extensions: 26140
Number of sequences better than 0.5: 9
Number of HSP's better than 0.5 without gapping: 9
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 26124
Number of HSP's gapped (non-prelim): 16
length of query: 691
length of database: 175,134,539
effective HSP length: 19
effective length of query: 672
effective length of database: 169,188,109
effective search space: 113694409248
effective search space used: 113694409248
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)