BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 8616263.2.1
(509 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI958400.1|BI958400 HVSMEn0014M17f Hordeum vulgare rachi... 100 9e-020
gb|BM370593.2|BM370593 EBro08_SQ004_L01_R root, 3 week, dro... 76 1e-012
gb|BI959364.1|BI959364 HVSMEn0019G04f Hordeum vulgare rachi... 58 3e-007
gb|BI956239.1|BI956239 HVSMEn0001G12f Hordeum vulgare rachi... 48 3e-004
gb|BI957064.1|BI957064 HVSMEn0007D21f Hordeum vulgare rachi... 48 3e-004
gb|BF256602.3|BF256602 HVSMEf0010H12f Hordeum vulgare seedl... 48 3e-004
gb|BG344484.2|BG344484 HVSMEg0009G13f Hordeum vulgare pre-a... 48 3e-004
gb|BQ461023.1|BQ461023 HE01H04r HE Hordeum vulgare subsp. v... 48 3e-004
gb|BQ659466.1|BQ659466 HE01H04u HE Hordeum vulgare subsp. v... 48 3e-004
gb|BE421314.1|BE421314 HWM008.A07 ITEC HWM Barley Leaf Libr... 42 0.017
gb|BF261416.3|BF261416 HV_CEa0001D16f Hordeum vulgare seedl... 38 0.27
gb|BM816736.1|BM816736 HB02C09_T3.ab1 HB Hordeum vulgare su... 38 0.27
gb|BU994261.1|BU994261 HM06G15r HM Hordeum vulgare subsp. v... 38 0.27
gb|BU997607.1|BU997607 HI08H17r HI Hordeum vulgare subsp. v... 38 0.27
>gb|BI958400.1|BI958400 HVSMEn0014M17f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0014M17f, mRNA sequence
Length = 677
Score = 99.6 bits (50), Expect = 9e-020
Identities = 95/110 (86%)
Strand = Plus / Minus
Query: 400 gggttgtcggcgaggaagcggatggccacccatccgccggccggcacgccgacggtgttg 459
||||||||||||||||| || | ||| ||||| ||| ||| ||||||| |||||||||
Sbjct: 645 gggttgtcggcgaggaaccgcacggcgacccagccggcggtgggcacgctgacggtgttc 586
Query: 460 cgctcgacggggtcgacgaggttgaacttggacgggtcgttgacggggtc 509
|| ||||||||||||||||||||| |||||| ||| ||||||| ||||||
Sbjct: 585 cgttcgacggggtcgacgaggttgtacttggccggatcgttgatggggtc 536
>gb|BM370593.2|BM370593 EBro08_SQ004_L01_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ004_L01 5', mRNA sequence
Length = 655
Score = 75.8 bits (38), Expect = 1e-012
Identities = 95/114 (83%)
Strand = Plus / Minus
Query: 370 tccaaatggcaatgcatgaaccaaaccccggggttgtcggcgaggaagcggatggccacc 429
||||| ||||| ||||||| ||| || |||||||||| ||||| |||||||||||| |||
Sbjct: 411 tccaagtggcagtgcatgatccacacaccggggttgttggcgacgaagcggatggcgacc 352
Query: 430 catccgccggccggcacgccgacggtgttgcgctcgacggggtcgacgaggttg 483
|| ||| ||| ||||||| || |||||| |||| | |||||||||||||||
Sbjct: 351 cagccggcggtgggcacgctgatggtgttccgctgctccgggtcgacgaggttg 298
>gb|BI959364.1|BI959364 HVSMEn0019G04f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0019G04f, mRNA sequence
Length = 673
Score = 58.0 bits (29), Expect = 3e-007
Identities = 38/41 (92%)
Strand = Plus / Minus
Query: 457 ttgcgctcgacggggtcgacgaggttgaacttggacgggtc 497
|||||||||||||||||||||||| || |||||| ||||||
Sbjct: 672 ttgcgctcgacggggtcgacgagggtgtacttggccgggtc 632
>gb|BI956239.1|BI956239 HVSMEn0001G12f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0001G12f, mRNA sequence
Length = 597
Score = 48.1 bits (24), Expect = 3e-004
Identities = 57/68 (83%)
Strand = Plus / Minus
Query: 442 ggcacgccgacggtgttgcgctcgacggggtcgacgaggttgaacttggacgggtcgttg 501
||||| |||||||||| ||| | ||||||||||| ||||||| | || |||||||| |
Sbjct: 589 ggcaccccgacggtgtcgcggttgacggggtcgaacaggttgacttgggccgggtcgtgg 530
Query: 502 acggggtc 509
||||||||
Sbjct: 529 acggggtc 522
>gb|BI957064.1|BI957064 HVSMEn0007D21f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0007D21f, mRNA sequence
Length = 626
Score = 48.1 bits (24), Expect = 3e-004
Identities = 63/76 (82%)
Strand = Plus / Plus
Query: 269 aacatttgggtaagtctgacggcggcgggagcagcttctggtgcgggaggctgccgtcga 328
|||||||||| | || |||||||| ||||||||||| || | ||||||||| |||||
Sbjct: 389 aacatttggggagatcggacggcggagggagcagcttttgttccgggaggctcccgtcct 448
Query: 329 gcaccaaccatgccat 344
|||||| ||| |||||
Sbjct: 449 gcaccacccacgccat 464
>gb|BF256602.3|BF256602 HVSMEf0010H12f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0010H12f, mRNA sequence
Length = 667
Score = 48.1 bits (24), Expect = 3e-004
Identities = 69/84 (82%)
Strand = Plus / Minus
Query: 400 gggttgtcggcgaggaagcggatggccacccatccgccggccggcacgccgacggtgttg 459
||||||| ||||| ||| |||||| | |||| || |||| |||||| ||| |||||||
Sbjct: 233 gggttgttggcgacgaatcggatgacggcccagcccccggtgggcacggcgatggtgttg 174
Query: 460 cgctcgacggggtcgacgaggttg 483
|| | ||||||||||||||||||
Sbjct: 173 cgggccacggggtcgacgaggttg 150
>gb|BG344484.2|BG344484 HVSMEg0009G13f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0009G13f, mRNA sequence
Length = 626
Score = 48.1 bits (24), Expect = 3e-004
Identities = 69/84 (82%)
Strand = Plus / Minus
Query: 400 gggttgtcggcgaggaagcggatggccacccatccgccggccggcacgccgacggtgttg 459
||||||| ||||| ||| |||||| | |||| || |||| |||||| ||| |||||||
Sbjct: 120 gggttgttggcgacgaatcggatgacggcccagcccccggtgggcacggcgatggtgttg 61
Query: 460 cgctcgacggggtcgacgaggttg 483
|| | ||||||||||||||||||
Sbjct: 60 cgggccacggggtcgacgaggttg 37
>gb|BQ461023.1|BQ461023 HE01H04r HE Hordeum vulgare subsp. vulgare cDNA clone HE01H04
5-PRIME, mRNA sequence
Length = 463
Score = 48.1 bits (24), Expect = 3e-004
Identities = 69/84 (82%)
Strand = Plus / Minus
Query: 400 gggttgtcggcgaggaagcggatggccacccatccgccggccggcacgccgacggtgttg 459
||||||| ||||| ||| |||||| | |||| || |||| |||||| ||| |||||||
Sbjct: 454 gggttgttggcgacgaatcggatgacggcccagcccccggtgggcacggcgatggtgttg 395
Query: 460 cgctcgacggggtcgacgaggttg 483
|| | ||||||||||||||||||
Sbjct: 394 cgggccacggggtcgacgaggttg 371
>gb|BQ659466.1|BQ659466 HE01H04u HE Hordeum vulgare subsp. vulgare cDNA clone HE01H04
3-PRIME, mRNA sequence
Length = 547
Score = 48.1 bits (24), Expect = 3e-004
Identities = 69/84 (82%)
Strand = Plus / Plus
Query: 400 gggttgtcggcgaggaagcggatggccacccatccgccggccggcacgccgacggtgttg 459
||||||| ||||| ||| |||||| | |||| || |||| |||||| ||| |||||||
Sbjct: 416 gggttgttggcgacgaatcggatgacggcccagcccccggtgggcacggcgatggtgttg 475
Query: 460 cgctcgacggggtcgacgaggttg 483
|| | ||||||||||||||||||
Sbjct: 476 cgggccacggggtcgacgaggttg 499
>gb|BE421314.1|BE421314 HWM008.A07 ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM008.A07, mRNA sequence
Length = 905
Score = 42.1 bits (21), Expect = 0.017
Identities = 43/49 (87%), Gaps = 1/49 (2%)
Strand = Plus / Minus
Query: 375 atggcaatgcatgaaccaaaccccggggttgtcggcgaggaagcggatg 423
|||||| ||||||| ||| ||||| |||||||||||| |||| ||||||
Sbjct: 579 atggcagtgcatga-ccacacccccgggttgtcggcgcggaaccggatg 532
>gb|BF261416.3|BF261416 HV_CEa0001D16f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0001D16f, mRNA sequence
Length = 951
Score = 38.2 bits (19), Expect = 0.27
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 435 gccggccggcacgccgacg 453
|||||||||||||||||||
Sbjct: 56 gccggccggcacgccgacg 74
>gb|BM816736.1|BM816736 HB02C09_T3.ab1 HB Hordeum vulgare subsp. vulgare cDNA clone
HB02C09_T3.ab1, mRNA sequence
Length = 862
Score = 38.2 bits (19), Expect = 0.27
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 435 gccggccggcacgccgacg 453
|||||||||||||||||||
Sbjct: 66 gccggccggcacgccgacg 84
>gb|BU994261.1|BU994261 HM06G15r HM Hordeum vulgare subsp. vulgare cDNA clone HM06G15
5-PRIME, mRNA sequence
Length = 349
Score = 38.2 bits (19), Expect = 0.27
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 435 gccggccggcacgccgacg 453
|||||||||||||||||||
Sbjct: 67 gccggccggcacgccgacg 85
>gb|BU997607.1|BU997607 HI08H17r HI Hordeum vulgare subsp. vulgare cDNA clone HI08H17
5-PRIME, mRNA sequence
Length = 568
Score = 38.2 bits (19), Expect = 0.27
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 435 gccggccggcacgccgacg 453
|||||||||||||||||||
Sbjct: 69 gccggccggcacgccgacg 87
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 58,937
Number of Sequences: 312970
Number of extensions: 58937
Number of successful extensions: 17290
Number of sequences better than 0.5: 14
Number of HSP's better than 0.5 without gapping: 14
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 17273
Number of HSP's gapped (non-prelim): 16
length of query: 509
length of database: 175,134,539
effective HSP length: 18
effective length of query: 491
effective length of database: 169,501,079
effective search space: 83225029789
effective search space used: 83225029789
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)