BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 7987759.3.1
(658 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI957606.1|BI957606 HVSMEn0010F15f Hordeum vulgare rachi... 58 4e-007
gb|BI957639.1|BI957639 HVSMEn0010H12f Hordeum vulgare rachi... 58 4e-007
gb|BI958645.1|BI958645 HVSMEn0016A21f Hordeum vulgare rachi... 58 4e-007
gb|BI959987.1|BI959987 HVSMEn0022M02f Hordeum vulgare rachi... 58 4e-007
gb|BI960501.1|BI960501 HVSMEn0024N01f Hordeum vulgare rachi... 58 4e-007
gb|BG343272.2|BG343272 HVSMEg0005E13f Hordeum vulgare pre-a... 58 4e-007
gb|BG344915.2|BG344915 HVSMEg0016N12f Hordeum vulgare pre-a... 58 4e-007
gb|BI947878.1|BI947878 HVSMEl0007E24f Hordeum vulgare spike... 56 2e-006
gb|BI950884.1|BI950884 HVSMEl0023I12f Hordeum vulgare spike... 56 2e-006
gb|CV054774.1|CV054774 BNEL113b6 Barley EST endosperm libra... 56 2e-006
gb|BI956571.1|BI956571 HVSMEn0004D06f Hordeum vulgare rachi... 50 9e-005
gb|AV913364.1|AV913364 AV913364 K. Sato unpublished cDNA li... 46 0.001
gb|AV918663.1|AV918663 AV918663 K. Sato unpublished cDNA li... 46 0.001
gb|BF267570.2|BF267570 HV_CEa0018E22f Hordeum vulgare seedl... 42 0.023
gb|BF621230.2|BF621230 HVSMEa0006C03f Hordeum vulgare seedl... 40 0.089
gb|BF253443.2|BF253443 HVSMEf0001G23f Hordeum vulgare seedl... 40 0.089
gb|AW982411.2|AW982411 HVSMEg0003D05f Hordeum vulgare pre-a... 40 0.089
gb|BI946630.1|BI946630 HVSMEl0001F01f Hordeum vulgare spike... 40 0.089
gb|BI949030.1|BI949030 HVSMEl0012B14f Hordeum vulgare spike... 40 0.089
gb|BI949033.1|BI949033 HVSMEl0012B18f Hordeum vulgare spike... 40 0.089
gb|BI949811.1|BI949811 HVSMEl0016D04f Hordeum vulgare spike... 40 0.089
gb|BI949816.1|BI949816 HVSMEl0016D14f Hordeum vulgare spike... 40 0.089
gb|BI951913.1|BI951913 HVSMEm0003K22f Hordeum vulgare green... 40 0.089
gb|BI952932.1|BI952932 HVSMEm0008I01f Hordeum vulgare green... 40 0.089
gb|BQ471085.1|BQ471085 HX04O17r HX Hordeum vulgare subsp. v... 40 0.089
gb|AL510282.1|AL510282 AL510282 Hordeum vulgare Barke devel... 38 0.35
gb|BF256222.2|BF256222 HVSMEf0009C22f Hordeum vulgare seedl... 38 0.35
gb|BI957257.1|BI957257 HVSMEn0008H11f Hordeum vulgare rachi... 38 0.35
gb|BF266773.1|BF266773 HV_CEa0016A09f Hordeum vulgare seedl... 38 0.35
gb|BQ467781.1|BQ467781 HS04O05r HS Hordeum vulgare subsp. v... 38 0.35
>gb|BI957606.1|BI957606 HVSMEn0010F15f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0010F15f, mRNA sequence
Length = 648
Score = 58.0 bits (29), Expect = 4e-007
Identities = 59/69 (85%)
Strand = Plus / Plus
Query: 257 acgctccacccgtccaagacaccatgcctgcggcgcctcatgcgcgtgctcaccgtcacc 316
||||||||||||||||||| |||||||| ||||| ||||| |||||||| ||| ||
Sbjct: 207 acgctccacccgtccaagatctgctgcctgcgccgccttatgcgtgtgctcactgtctcc 266
Query: 317 ggcgtcttc 325
|||||||||
Sbjct: 267 ggcgtcttc 275
>gb|BI957639.1|BI957639 HVSMEn0010H12f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0010H12f, mRNA sequence
Length = 652
Score = 58.0 bits (29), Expect = 4e-007
Identities = 59/69 (85%)
Strand = Plus / Plus
Query: 257 acgctccacccgtccaagacaccatgcctgcggcgcctcatgcgcgtgctcaccgtcacc 316
||||||||||||||||||| |||||||| ||||| ||||| |||||||| ||| ||
Sbjct: 194 acgctccacccgtccaagatctgctgcctgcgccgccttatgcgtgtgctcactgtctcc 253
Query: 317 ggcgtcttc 325
|||||||||
Sbjct: 254 ggcgtcttc 262
>gb|BI958645.1|BI958645 HVSMEn0016A21f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0016A21f, mRNA sequence
Length = 624
Score = 58.0 bits (29), Expect = 4e-007
Identities = 59/69 (85%)
Strand = Plus / Plus
Query: 257 acgctccacccgtccaagacaccatgcctgcggcgcctcatgcgcgtgctcaccgtcacc 316
||||||||||||||||||| |||||||| ||||| ||||| |||||||| ||| ||
Sbjct: 214 acgctccacccgtccaagatctgctgcctgcgccgccttatgcgtgtgctcactgtctcc 273
Query: 317 ggcgtcttc 325
|||||||||
Sbjct: 274 ggcgtcttc 282
>gb|BI959987.1|BI959987 HVSMEn0022M02f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0022M02f, mRNA sequence
Length = 629
Score = 58.0 bits (29), Expect = 4e-007
Identities = 59/69 (85%)
Strand = Plus / Plus
Query: 257 acgctccacccgtccaagacaccatgcctgcggcgcctcatgcgcgtgctcaccgtcacc 316
||||||||||||||||||| |||||||| ||||| ||||| |||||||| ||| ||
Sbjct: 207 acgctccacccgtccaagatctgctgcctgcgccgccttatgcgtgtgctcactgtctcc 266
Query: 317 ggcgtcttc 325
|||||||||
Sbjct: 267 ggcgtcttc 275
>gb|BI960501.1|BI960501 HVSMEn0024N01f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0024N01f, mRNA sequence
Length = 660
Score = 58.0 bits (29), Expect = 4e-007
Identities = 59/69 (85%)
Strand = Plus / Plus
Query: 257 acgctccacccgtccaagacaccatgcctgcggcgcctcatgcgcgtgctcaccgtcacc 316
||||||||||||||||||| |||||||| ||||| ||||| |||||||| ||| ||
Sbjct: 206 acgctccacccgtccaagatctgctgcctgcgccgccttatgcgtgtgctcactgtctcc 265
Query: 317 ggcgtcttc 325
|||||||||
Sbjct: 266 ggcgtcttc 274
>gb|BG343272.2|BG343272 HVSMEg0005E13f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0005E13f, mRNA sequence
Length = 992
Score = 58.0 bits (29), Expect = 4e-007
Identities = 59/69 (85%)
Strand = Plus / Plus
Query: 257 acgctccacccgtccaagacaccatgcctgcggcgcctcatgcgcgtgctcaccgtcacc 316
||||||||||||||||||| |||||||| ||||| ||||| |||||||| ||| ||
Sbjct: 212 acgctccacccgtccaagatctgctgcctgcgccgccttatgcgtgtgctcactgtctcc 271
Query: 317 ggcgtcttc 325
|||||||||
Sbjct: 272 ggcgtcttc 280
>gb|BG344915.2|BG344915 HVSMEg0016N12f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0016N12f, mRNA sequence
Length = 635
Score = 58.0 bits (29), Expect = 4e-007
Identities = 59/69 (85%)
Strand = Plus / Plus
Query: 257 acgctccacccgtccaagacaccatgcctgcggcgcctcatgcgcgtgctcaccgtcacc 316
||||||||||||||||||| |||||||| ||||| ||||| |||||||| ||| ||
Sbjct: 213 acgctccacccgtccaagatctgctgcctgcgccgccttatgcgtgtgctcactgtctcc 272
Query: 317 ggcgtcttc 325
|||||||||
Sbjct: 273 ggcgtcttc 281
>gb|BI947878.1|BI947878 HVSMEl0007E24f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0007E24f, mRNA sequence
Length = 529
Score = 56.0 bits (28), Expect = 2e-006
Identities = 40/44 (90%)
Strand = Plus / Plus
Query: 129 agctgtggaaccacaccttcggctacgtcaagtccatggcgctc 172
|||| ||||||| ||||||||| ||| |||||||||||||||||
Sbjct: 53 agctctggaacctcaccttcggttacctcaagtccatggcgctc 96
>gb|BI950884.1|BI950884 HVSMEl0023I12f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0023I12f, mRNA sequence
Length = 958
Score = 56.0 bits (28), Expect = 2e-006
Identities = 40/44 (90%)
Strand = Plus / Plus
Query: 129 agctgtggaaccacaccttcggctacgtcaagtccatggcgctc 172
|||| ||||||| ||||||||| ||| |||||||||||||||||
Sbjct: 53 agctctggaacctcaccttcggttacctcaagtccatggcgctc 96
>gb|CV054774.1|CV054774 BNEL113b6 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL113b6 5' similar to catechol
O-methyltransferase, mRNA sequence
Length = 590
Score = 56.0 bits (28), Expect = 2e-006
Identities = 40/44 (90%)
Strand = Plus / Plus
Query: 129 agctgtggaaccacaccttcggctacgtcaagtccatggcgctc 172
|||| ||||||| ||||||||| ||| |||||||||||||||||
Sbjct: 68 agctctggaacctcaccttcggttacctcaagtccatggcgctc 111
>gb|BI956571.1|BI956571 HVSMEn0004D06f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0004D06f, mRNA sequence
Length = 780
Score = 50.1 bits (25), Expect = 9e-005
Identities = 40/45 (88%)
Strand = Plus / Plus
Query: 281 tgcctgcggcgcctcatgcgcgtgctcaccgtcaccggcgtcttc 325
|||||||| ||||| ||||| |||||||| ||| |||||||||||
Sbjct: 12 tgcctgcgccgccttatgcgtgtgctcactgtctccggcgtcttc 56
>gb|AV913364.1|AV913364 AV913364 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags21m21 5', mRNA sequence
Length = 665
Score = 46.1 bits (23), Expect = 0.001
Identities = 44/51 (86%)
Strand = Plus / Plus
Query: 606 atggtcgcggacagcggcttcatcatgaacgtcgtggtcagggagtgcggc 656
|||||| |||||||| ||||||||||| || ||| |||| ||||||||||
Sbjct: 168 atggtctcggacagccgcttcatcatggacatcgccgtcaaggagtgcggc 218
>gb|AV918663.1|AV918663 AV918663 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags21m21 3', mRNA sequence
Length = 612
Score = 46.1 bits (23), Expect = 0.001
Identities = 44/51 (86%)
Strand = Plus / Minus
Query: 606 atggtcgcggacagcggcttcatcatgaacgtcgtggtcagggagtgcggc 656
|||||| |||||||| ||||||||||| || ||| |||| ||||||||||
Sbjct: 457 atggtctcggacagccgcttcatcatggacatcgccgtcaaggagtgcggc 407
>gb|BF267570.2|BF267570 HV_CEa0018E22f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0018E22f, mRNA sequence
Length = 518
Score = 42.1 bits (21), Expect = 0.023
Identities = 26/28 (92%)
Strand = Plus / Plus
Query: 283 cctgcggcgcctcatgcgcgtgctcacc 310
||| ||||| ||||||||||||||||||
Sbjct: 215 cctncggcggctcatgcgcgtgctcacc 242
>gb|BF621230.2|BF621230 HVSMEa0006C03f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0006C03f, mRNA sequence
Length = 808
Score = 40.1 bits (20), Expect = 0.089
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 287 cggcgcctcatgcgcgtgctcacc 310
||||| ||||||||||||||||||
Sbjct: 222 cggcggctcatgcgcgtgctcacc 245
>gb|BF253443.2|BF253443 HVSMEf0001G23f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0001G23f, mRNA sequence
Length = 850
Score = 40.1 bits (20), Expect = 0.089
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 287 cggcgcctcatgcgcgtgctcacc 310
||||| ||||||||||||||||||
Sbjct: 210 cggcggctcatgcgcgtgctcacc 233
>gb|AW982411.2|AW982411 HVSMEg0003D05f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0003D05f, mRNA sequence
Length = 1041
Score = 40.1 bits (20), Expect = 0.089
Identities = 32/36 (88%)
Strand = Plus / Plus
Query: 290 cgcctcatgcgcgtgctcaccgtcaccggcgtcttc 325
||||| ||||| |||||||| ||| |||||||||||
Sbjct: 3 cgccttatgcgtgtgctcactgtctccggcgtcttc 38
>gb|BI946630.1|BI946630 HVSMEl0001F01f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0001F01f, mRNA sequence
Length = 585
Score = 40.1 bits (20), Expect = 0.089
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 287 cggcgcctcatgcgcgtgctcacc 310
||||| ||||||||||||||||||
Sbjct: 218 cggcggctcatgcgcgtgctcacc 241
>gb|BI949030.1|BI949030 HVSMEl0012B14f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0012B14f, mRNA sequence
Length = 972
Score = 40.1 bits (20), Expect = 0.089
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 287 cggcgcctcatgcgcgtgctcacc 310
||||| ||||||||||||||||||
Sbjct: 219 cggcggctcatgcgcgtgctcacc 242
>gb|BI949033.1|BI949033 HVSMEl0012B18f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0012B18f, mRNA sequence
Length = 966
Score = 40.1 bits (20), Expect = 0.089
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 287 cggcgcctcatgcgcgtgctcacc 310
||||| ||||||||||||||||||
Sbjct: 218 cggcggctcatgcgcgtgctcacc 241
>gb|BI949811.1|BI949811 HVSMEl0016D04f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0016D04f, mRNA sequence
Length = 542
Score = 40.1 bits (20), Expect = 0.089
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 287 cggcgcctcatgcgcgtgctcacc 310
||||| ||||||||||||||||||
Sbjct: 210 cggcggctcatgcgcgtgctcacc 233
>gb|BI949816.1|BI949816 HVSMEl0016D14f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0016D14f, mRNA sequence
Length = 530
Score = 40.1 bits (20), Expect = 0.089
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 287 cggcgcctcatgcgcgtgctcacc 310
||||| ||||||||||||||||||
Sbjct: 209 cggcggctcatgcgcgtgctcacc 232
>gb|BI951913.1|BI951913 HVSMEm0003K22f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0003K22f, mRNA sequence
Length = 866
Score = 40.1 bits (20), Expect = 0.089
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 287 cggcgcctcatgcgcgtgctcacc 310
||||| ||||||||||||||||||
Sbjct: 206 cggcggctcatgcgcgtgctcacc 229
>gb|BI952932.1|BI952932 HVSMEm0008I01f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0008I01f, mRNA sequence
Length = 1339
Score = 40.1 bits (20), Expect = 0.089
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 287 cggcgcctcatgcgcgtgctcacc 310
||||| ||||||||||||||||||
Sbjct: 219 cggcggctcatgcgcgtgctcacc 242
>gb|BQ471085.1|BQ471085 HX04O17r HX Hordeum vulgare subsp. vulgare cDNA clone HX04O17
5-PRIME, mRNA sequence
Length = 374
Score = 40.1 bits (20), Expect = 0.089
Identities = 23/24 (95%)
Strand = Plus / Minus
Query: 96 gcgaacagcaggtggcgctggatg 119
|||||||||||||||||| |||||
Sbjct: 345 gcgaacagcaggtggcgcgggatg 322
>gb|AL510282.1|AL510282 AL510282 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY04H10u 3',
mRNA sequence
Length = 605
Score = 38.2 bits (19), Expect = 0.35
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 300 gcgtgctcaccgtcaccgg 318
|||||||||||||||||||
Sbjct: 491 gcgtgctcaccgtcaccgg 509
>gb|BF256222.2|BF256222 HVSMEf0009C22f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0009C22f, mRNA sequence
Length = 762
Score = 38.2 bits (19), Expect = 0.35
Identities = 22/23 (95%)
Strand = Plus / Plus
Query: 52 ggagcaaaaactcacaatttctg 74
||||||||| |||||||||||||
Sbjct: 400 ggagcaaaatctcacaatttctg 422
>gb|BI957257.1|BI957257 HVSMEn0008H11f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0008H11f, mRNA sequence
Length = 446
Score = 38.2 bits (19), Expect = 0.35
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 257 acgctccacccgtccaaga 275
|||||||||||||||||||
Sbjct: 214 acgctccacccgtccaaga 232
>gb|BF266773.1|BF266773 HV_CEa0016A09f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0016A09f, mRNA sequence
Length = 858
Score = 38.2 bits (19), Expect = 0.35
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 300 gcgtgctcaccgtcaccgg 318
|||||||||||||||||||
Sbjct: 480 gcgtgctcaccgtcaccgg 498
>gb|BQ467781.1|BQ467781 HS04O05r HS Hordeum vulgare subsp. vulgare cDNA clone HS04O05
5-PRIME, mRNA sequence
Length = 478
Score = 38.2 bits (19), Expect = 0.35
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 94 cagcgaacagcaggtggcg 112
|||||||||||||||||||
Sbjct: 405 cagcgaacagcaggtggcg 387
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 90,437
Number of Sequences: 312970
Number of extensions: 90437
Number of successful extensions: 25655
Number of sequences better than 0.5: 30
Number of HSP's better than 0.5 without gapping: 30
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 25563
Number of HSP's gapped (non-prelim): 85
length of query: 658
length of database: 175,134,539
effective HSP length: 19
effective length of query: 639
effective length of database: 169,188,109
effective search space: 108111201651
effective search space used: 108111201651
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)