BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 5705280.3.1
(980 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF621515.2|BF621515 HVSMEa0011D23f Hordeum vulgare seedl... 141 5e-032
gb|BE195366.3|BE195366 HVSMEh0089A15f Hordeum vulgare 5-45 ... 38 0.53
gb|AL499994.1|AL499994 AL499994 Hordeum vulgare Barke etiol... 36 2.1
gb|AV837121.1|AV837121 AV837121 K. Sato unpublished cDNA li... 36 2.1
gb|BQ468765.1|BQ468765 HM02E11r HM Hordeum vulgare subsp. v... 36 2.1
gb|BI777009.2|BI777009 EBpi03_SQ001_K07_R pistil, 4 DPA, no... 36 2.1
gb|CA005670.1|CA005670 HU09D03u HU Hordeum vulgare subsp. v... 36 2.1
gb|CA032632.1|CA032632 HX13L17r HX Hordeum vulgare subsp. v... 36 2.1
gb|CB876271.1|CB876271 HX10M16w HX Hordeum vulgare subsp. v... 36 2.1
gb|BF255820.2|BF255820 HVSMEf0007O13f Hordeum vulgare seedl... 34 8.3
gb|BJ450002.1|BJ450002 BJ450002 K. Sato unpublished cDNA li... 34 8.3
gb|BQ465419.1|BQ465419 HU03J13r HU Hordeum vulgare subsp. v... 34 8.3
gb|BQ465751.1|BQ465751 HU04J11r HU Hordeum vulgare subsp. v... 34 8.3
gb|CV063400.1|CV063400 BNEL89h3 Barley EST endosperm librar... 34 8.3
gb|AF414175.1|AF414175 Hordeum vulgare clone pic20E NBS-LRR... 34 8.3
>gb|BF621515.2|BF621515 HVSMEa0011D23f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0011D23f, mRNA sequence
Length = 806
Score = 141 bits (71), Expect = 5e-032
Identities = 192/231 (83%), Gaps = 1/231 (0%)
Strand = Plus / Plus
Query: 182 ctagcgttaaccttgaggggaccgtcttcacattctcagacacaaggaacaagttcacag 241
||||| ||| |||||||||||| ||||| |||||| || ||||| |||||||||||||
Sbjct: 8 ctagctttagccttgaggggacaagcttca-attctccgatacaagaaacaagttcacag 66
Query: 242 ctctgggttgtaacgtggtggccatgcttttgaatggcagcacggggtatagcggtggct 301
|| |||||||| | ||||||||||||| |||||||| |||| | |||||||||||||
Sbjct: 67 ctgtgggttgtgatatggtggccatgctgttgaatggtagcagtgattatagcggtggct 126
Query: 302 gtgcttccttttgctctagtggaaataacatcatcgacggctcatgctctggggtggctt 361
||||||| ||||||||| ||||| |||| || | || || |||||||| |||||||
Sbjct: 127 gtgcttctctttgctctacaaaaaatagcatcgtcaatggttcttgctctggtgtggctt 186
Query: 362 gctgccaagcgccggttcccaaagggctaaagaagctggacttagagttca 412
|||||||||| ||||| ||||| ||| ||||||||||| ||||||||||
Sbjct: 187 gctgccaagcaccggtacccaaggggttaaagaagctgtctttagagttca 237
>gb|BE195366.3|BE195366 HVSMEh0089A15f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0089A15f, mRNA sequence
Length = 848
Score = 38.2 bits (19), Expect = 0.53
Identities = 37/43 (86%)
Strand = Plus / Plus
Query: 925 ttctggactcactggcttggtaagaagaggaaacttgcaaaga 967
|||||||||||||| |||| ||||| ||| || ||||| ||||
Sbjct: 52 ttctggactcactgtcttgttaagaggagaaagcttgcgaaga 94
>gb|AL499994.1|AL499994 AL499994 Hordeum vulgare Barke etiolated leaves Hordeum vulgare
subsp. vulgare cDNA clone HK04G13r 3', mRNA sequence
Length = 611
Score = 36.2 bits (18), Expect = 2.1
Identities = 18/18 (100%)
Strand = Plus / Minus
Query: 823 ggcgacggcctcaaggaa 840
||||||||||||||||||
Sbjct: 343 ggcgacggcctcaaggaa 326
>gb|AV837121.1|AV837121 AV837121 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd26m18, mRNA
sequence
Length = 635
Score = 36.2 bits (18), Expect = 2.1
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 737 atgaatgcatcaccagaa 754
||||||||||||||||||
Sbjct: 198 atgaatgcatcaccagaa 215
>gb|BQ468765.1|BQ468765 HM02E11r HM Hordeum vulgare subsp. vulgare cDNA clone HM02E11
5-PRIME, mRNA sequence
Length = 333
Score = 36.2 bits (18), Expect = 2.1
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 823 ggcgacggcctcaaggaa 840
||||||||||||||||||
Sbjct: 65 ggcgacggcctcaaggaa 82
>gb|BI777009.2|BI777009 EBpi03_SQ001_K07_R pistil, 4 DPA, no treatment, cv Optic, EBpi03
Hordeum vulgare subsp. vulgare cDNA clone
EBpi03_SQ001_K07 5', mRNA sequence
Length = 470
Score = 36.2 bits (18), Expect = 2.1
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 823 ggcgacggcctcaaggaa 840
||||||||||||||||||
Sbjct: 267 ggcgacggcctcaaggaa 284
>gb|CA005670.1|CA005670 HU09D03u HU Hordeum vulgare subsp. vulgare cDNA clone HU09D03
3-PRIME, mRNA sequence
Length = 442
Score = 36.2 bits (18), Expect = 2.1
Identities = 18/18 (100%)
Strand = Plus / Minus
Query: 823 ggcgacggcctcaaggaa 840
||||||||||||||||||
Sbjct: 440 ggcgacggcctcaaggaa 423
>gb|CA032632.1|CA032632 HX13L17r HX Hordeum vulgare subsp. vulgare cDNA clone HX13L17
5-PRIME, mRNA sequence
Length = 366
Score = 36.2 bits (18), Expect = 2.1
Identities = 18/18 (100%)
Strand = Plus / Plus
Query: 823 ggcgacggcctcaaggaa 840
||||||||||||||||||
Sbjct: 91 ggcgacggcctcaaggaa 108
>gb|CB876271.1|CB876271 HX10M16w HX Hordeum vulgare subsp. vulgare cDNA clone HX10M16
3-PRIME, mRNA sequence
Length = 625
Score = 36.2 bits (18), Expect = 2.1
Identities = 18/18 (100%)
Strand = Plus / Minus
Query: 823 ggcgacggcctcaaggaa 840
||||||||||||||||||
Sbjct: 338 ggcgacggcctcaaggaa 321
>gb|BF255820.2|BF255820 HVSMEf0007O13f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0007O13f, mRNA sequence
Length = 948
Score = 34.2 bits (17), Expect = 8.3
Identities = 17/17 (100%)
Strand = Plus / Plus
Query: 388 ctaaagaagctggactt 404
|||||||||||||||||
Sbjct: 207 ctaaagaagctggactt 223
>gb|BJ450002.1|BJ450002 BJ450002 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak27k04 5', mRNA sequence
Length = 529
Score = 34.2 bits (17), Expect = 8.3
Identities = 17/17 (100%)
Strand = Plus / Plus
Query: 388 ctaaagaagctggactt 404
|||||||||||||||||
Sbjct: 376 ctaaagaagctggactt 392
>gb|BQ465419.1|BQ465419 HU03J13r HU Hordeum vulgare subsp. vulgare cDNA clone HU03J13
5-PRIME, mRNA sequence
Length = 601
Score = 34.2 bits (17), Expect = 8.3
Identities = 17/17 (100%)
Strand = Plus / Plus
Query: 388 ctaaagaagctggactt 404
|||||||||||||||||
Sbjct: 530 ctaaagaagctggactt 546
>gb|BQ465751.1|BQ465751 HU04J11r HU Hordeum vulgare subsp. vulgare cDNA clone HU04J11
5-PRIME, mRNA sequence
Length = 621
Score = 34.2 bits (17), Expect = 8.3
Identities = 17/17 (100%)
Strand = Plus / Plus
Query: 388 ctaaagaagctggactt 404
|||||||||||||||||
Sbjct: 518 ctaaagaagctggactt 534
>gb|CV063400.1|CV063400 BNEL89h3 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL89h3 5' similar to putative
Glycine-rich protein 2, mRNA sequence
Length = 768
Score = 34.2 bits (17), Expect = 8.3
Identities = 20/21 (95%)
Strand = Plus / Minus
Query: 453 ctggccatgtggtcaggcctt 473
|||||||||||| ||||||||
Sbjct: 528 ctggccatgtggccaggcctt 508
>gb|AF414175.1|AF414175 Hordeum vulgare clone pic20E NBS-LRR-like protein gene, partial cds
Length = 1890
Score = 34.2 bits (17), Expect = 8.3
Identities = 17/17 (100%)
Strand = Plus / Minus
Query: 935 actggcttggtaagaag 951
|||||||||||||||||
Sbjct: 677 actggcttggtaagaag 661
Database: Hordeum_nucl_with_EST.fasta
Posted date: May 2, 2006 2:38 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 91,692
Number of Sequences: 312970
Number of extensions: 91692
Number of successful extensions: 23687
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 23667
Number of HSP's gapped (non-prelim): 18
length of query: 980
length of database: 175,134,539
effective HSP length: 19
effective length of query: 961
effective length of database: 169,188,109
effective search space: 162589772749
effective search space used: 162589772749
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 17 (34.2 bits)