BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 5704580.2.1
(721 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU974788.1|BU974788 HB29B20r BC Hordeum vulgare subsp. v... 115 2e-024
gb|BJ446848.1|BJ446848 BJ446848 K. Sato unpublished cDNA li... 40 0.098
gb|BJ454853.1|BJ454853 BJ454853 K. Sato unpublished cDNA li... 40 0.098
gb|BI957052.1|BI957052 HVSMEn0007C18f Hordeum vulgare rachi... 38 0.39
>gb|BU974788.1|BU974788 HB29B20r BC Hordeum vulgare subsp. vulgare cDNA clone HB29B20
5-PRIME, mRNA sequence
Length = 457
Score = 115 bits (58), Expect = 2e-024
Identities = 121/142 (85%)
Strand = Plus / Plus
Query: 236 actgcccaagcttcctccatatgcctttctttgctgtcaagcacttcaatttgccaaaca 295
|||| |||||||||| |||||||||||||||| |||| | |||||||||||| || ||||
Sbjct: 300 actgtccaagcttcccccatatgcctttctttactgttaggcacttcaatttaccgaaca 359
Query: 296 caaaaccggacttctgagggttgccatccacaagcgagttctcgtatatctctttgcatt 355
| || || ||||| |||| |||||||| | || |||||||| ||||| || |||||||
Sbjct: 360 cgaaccctgactttggaggattgccatctatgagagagttctcatatatttccttgcatt 419
Query: 356 cttgtacaacctccttggcttc 377
||||||| ||||||||||||||
Sbjct: 420 cttgtacgacctccttggcttc 441
>gb|BJ446848.1|BJ446848 BJ446848 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak15b04 5', mRNA sequence
Length = 281
Score = 40.1 bits (20), Expect = 0.098
Identities = 26/28 (92%)
Strand = Plus / Minus
Query: 236 actgcccaagcttcctccatatgccttt 263
|||| |||||||||| ||||||||||||
Sbjct: 43 actgtccaagcttcccccatatgccttt 16
>gb|BJ454853.1|BJ454853 BJ454853 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak15b04 3', mRNA sequence
Length = 253
Score = 40.1 bits (20), Expect = 0.098
Identities = 28/31 (90%)
Strand = Plus / Plus
Query: 236 actgcccaagcttcctccatatgcctttctt 266
|||| ||||| |||| |||||||||||||||
Sbjct: 222 actgtccaagnttcccccatatgcctttctt 252
>gb|BI957052.1|BI957052 HVSMEn0007C18f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0007C18f, mRNA sequence
Length = 1129
Score = 38.2 bits (19), Expect = 0.39
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 148 aaaagaacaaaagaatgat 166
|||||||||||||||||||
Sbjct: 425 aaaagaacaaaagaatgat 407
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 62,310
Number of Sequences: 312970
Number of extensions: 62310
Number of successful extensions: 17566
Number of sequences better than 0.5: 4
Number of HSP's better than 0.5 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 17559
Number of HSP's gapped (non-prelim): 7
length of query: 721
length of database: 175,134,539
effective HSP length: 19
effective length of query: 702
effective length of database: 169,188,109
effective search space: 118770052518
effective search space used: 118770052518
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)