BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4790360.2.1
(667 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG414688.2|BG414688 HVSMEk0003F21f Hordeum vulgare testa... 280 6e-074
gb|BG416042.1|BG416042 HVSMEk0009I22f Hordeum vulgare testa... 244 3e-063
gb|CK122677.1|CK122677 BES1824105a18 BES1824 Hordeum vulgar... 202 1e-050
>gb|BG414688.2|BG414688 HVSMEk0003F21f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0003F21f, mRNA sequence
Length = 625
Score = 280 bits (141), Expect = 6e-074
Identities = 225/253 (88%)
Strand = Plus / Plus
Query: 39 tccttcatgcatatgggccaatttatgctttatcagctgacgtggtggctagcttggttg 98
||||||||||||||||||| |||||||||||||||||||| |||||||| ||||||||||
Sbjct: 1 tccttcatgcatatgggcctatttatgctttatcagctgatgtggtggcaagcttggttg 60
Query: 99 cattgcgaaacaacagtttccgtatgttcagcaacgaggacgttacaattggatcgtgga 158
| || |||||||||||||||| ||||||| ||||||||||||||| |||||||| ||||
Sbjct: 61 ctctgagaaacaacagtttccgaatgttcaacaacgaggacgttaccattggatcctgga 120
Query: 159 tgcttgctatgaacgtcaaccatgagaacacacatgcactttgtgaacctgactgcacgg 218
|||||||||||||||||||||| |||||||| ||||| || || || || || |||||||
Sbjct: 121 tgcttgctatgaacgtcaaccacgagaacacccatgccctctgcgagcccgagtgcacgg 180
Query: 219 agtcttcaattgctgtttgggacatcccaaaatgctcaggtctatgccacccagaggtaa 278
|| || ||||| || ||||||||||||||||| ||||| |||||||||||||||||||
Sbjct: 181 catcctcgattgccgtctgggacatcccaaaatgttcagggctatgccacccagaggtaa 240
Query: 279 aaatgctagagct 291
| ||||| |||||
Sbjct: 241 agatgctggagct 253
>gb|BG416042.1|BG416042 HVSMEk0009I22f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0009I22f, mRNA sequence
Length = 1066
Score = 244 bits (123), Expect = 3e-063
Identities = 253/291 (86%), Gaps = 4/291 (1%)
Strand = Plus / Plus
Query: 4 tgagccccaatcctttttactcggatcacaatacttccttcatgcatatgggccaattta 63
|||||| || ||||| |||| |||||| ||||||||||||||||||||||||| |||||
Sbjct: 79 tgagccacagtccttcctacttggatcagaatacttccttcatgcatatgggcctattta 138
Query: 64 tgctttatcagctgacgtggtggctagcttggttgcattgcgaaacaacagtttccgtat 123
||||||||||||||| |||||||| ||||||||||| || |||||||||||||||| ||
Sbjct: 139 tgctttatcagctgatgtggtggcaagcttggttgctctgagaaacaacagtttccgaat 198
Query: 124 gttcagcaacgaggacgtt-acaattggatcgtggatgcttgctatgaacgtcaaccatg 182
||||| ||||||||||||| || |||||||| |||||||||||||||||||||||||| |
Sbjct: 199 gttcaacaacgaggacgttaaccattggatcctggatgcttgctatgaacgtcaaccacg 258
Query: 183 agaacacacatgcactttgtgaacctgactgc-acggagtcttcaattgctgtttgggac 241
||||| | ||||| ||||| || || || ||| ||||| || || ||||| | ||||||
Sbjct: 259 agaacccccatgccctttgcgagcccgagtgcaacggattc-tcgattgccgactgggac 317
Query: 242 atcccaaaatgctcaggtctatgcca-cccagaggtaaaaatgctagagct 291
||||||||||| ||||| |||||||| |||| ||||||| ||||| |||||
Sbjct: 318 atcccaaaatgttcagggctatgccaccccaaaggtaaagatgctggagct 368
>gb|CK122677.1|CK122677 BES1824105a18 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010A185 5-PRIME, mRNA sequence
Length = 627
Score = 202 bits (102), Expect = 1e-050
Identities = 162/182 (89%)
Strand = Plus / Plus
Query: 110 aacagtttccgtatgttcagcaacgaggacgttacaattggatcgtggatgcttgctatg 169
||||||||||| ||||||| ||||||||||||||| |||||||| |||||||||||||||
Sbjct: 1 aacagtttccgaatgttcaacaacgaggacgttaccattggatcctggatgcttgctatg 60
Query: 170 aacgtcaaccatgagaacacacatgcactttgtgaacctgactgcacggagtcttcaatt 229
|||||||||||||||||||| ||||| || || ||||| || ||||||| || || |||
Sbjct: 61 aacgtcaaccatgagaacacccatgccctctgcgaacccgagtgcacggcatcctcgatt 120
Query: 230 gctgtttgggacatcccaaaatgctcaggtctatgccacccagaggtaaaaatgctagag 289
|| || ||||||||||||||||| ||||| |||||||||||||||||||| ||||| |||
Sbjct: 121 gccgtctgggacatcccaaaatgttcagggctatgccacccagaggtaaagatgctggag 180
Query: 290 ct 291
||
Sbjct: 181 ct 182
Score = 38.2 bits (19), Expect = 0.36
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 636 agttttgttgtatagaaga 654
|||||||||||||||||||
Sbjct: 573 agttttgttgtatagaaga 591
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 47,583
Number of Sequences: 312970
Number of extensions: 47583
Number of successful extensions: 12759
Number of sequences better than 0.5: 3
Number of HSP's better than 0.5 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 12753
Number of HSP's gapped (non-prelim): 5
length of query: 667
length of database: 175,134,539
effective HSP length: 19
effective length of query: 648
effective length of database: 169,188,109
effective search space: 109633894632
effective search space used: 109633894632
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)