BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3977955.2.1
(867 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ754059.1|BQ754059 EBca01_SQ002_L07_R carpel, pre-anthe... 90 1e-016
gb|BI950935.1|BI950935 HVSMEl0023L12f Hordeum vulgare spike... 86 2e-015
gb|BI780849.2|BI780849 EBma05_SQ001_C24_R maternal, 12 DPA,... 86 2e-015
gb|BJ465418.1|BJ465418 BJ465418 K. Sato unpublished cDNA li... 54 8e-006
gb|CK122193.1|CK122193 BES1824101p15 BES1824 Hordeum vulgar... 48 5e-004
gb|CK122985.1|CK122985 BES1824101k13 BES1824 Hordeum vulgar... 48 5e-004
gb|BG415761.2|BG415761 HVSMEk0007L15f Hordeum vulgare testa... 40 0.12
gb|BM442638.2|BM442638 EBan01_SQ003_L22_R anther, yellow st... 40 0.12
gb|CA029858.1|CA029858 HX05G15r HX Hordeum vulgare subsp. v... 40 0.12
gb|BF619063.2|BF619063 HVSMEc0008J10f Hordeum vulgare seedl... 38 0.47
gb|CV061819.1|CV061819 BNEL73c12 Barley EST endosperm libra... 38 0.47
>gb|BQ754059.1|BQ754059 EBca01_SQ002_L07_R carpel, pre-anthesis, no treatment, cv Optic,
EBca01 Hordeum vulgare subsp. vulgare cDNA clone
EBca01_SQ002_L07 5', mRNA sequence
Length = 372
Score = 89.7 bits (45), Expect = 1e-016
Identities = 93/109 (85%)
Strand = Plus / Minus
Query: 759 gttgccgaagatgaagtcgatggtgccggtcacccggcagtcgcggaagaactgccgctg 818
||||||||||| |||||||| |||||||| || |||||||||||| ||||||| || |
Sbjct: 196 gttgccgaagacgaagtcgacggtgccggcgacgcggcagtcgcggtagaactggcggag 137
Query: 819 cgcgtgcgtgtacagcgtgtcctggtagccgtcgaaggtgcactggtag 867
|| ||||| ||||||||||||||||| || ||||||| |||| |||||
Sbjct: 136 cgagtgcgcgtacagcgtgtcctggtgcccctcgaaggcgcaccggtag 88
>gb|BI950935.1|BI950935 HVSMEl0023L12f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0023L12f, mRNA sequence
Length = 536
Score = 85.7 bits (43), Expect = 2e-015
Identities = 76/87 (87%)
Strand = Plus / Minus
Query: 757 gagttgccgaagatgaagtcgatggtgccggtcacccggcagtcgcggaagaactgccgc 816
||||||||||||||||||||||||||||||| | ||||| ||||||||||||||||
Sbjct: 141 gagttgccgaagatgaagtcgatggtgccggagatgacgcagttgcggaagaactgccgc 82
Query: 817 tgcgcgtgcgtgtacagcgtgtcctgg 843
| |||||| ||||||||||||||||
Sbjct: 81 cgggcgtgcacgtacagcgtgtcctgg 55
Score = 56.0 bits (28), Expect = 2e-006
Identities = 58/68 (85%)
Strand = Plus / Minus
Query: 422 cggcgccgtcgccgcggttgtccacctccgcgtagaagagcgtctcgaggccgaagtcgc 481
|||||||| |||||||||| | ||| |||||| ||||||||| ||| ||||||||||
Sbjct: 473 cggcgccggggccgcggttgccgtactcggcgtagtagagcgtcttgagcccgaagtcgc 414
Query: 482 cgttccac 489
||||||||
Sbjct: 413 cgttccac 406
>gb|BI780849.2|BI780849 EBma05_SQ001_C24_R maternal, 12 DPA, no treatment, cv Optic, EBma05
Hordeum vulgare subsp. vulgare cDNA clone
EBma05_SQ001_C24 5', mRNA sequence
Length = 602
Score = 85.7 bits (43), Expect = 2e-015
Identities = 76/87 (87%)
Strand = Plus / Minus
Query: 757 gagttgccgaagatgaagtcgatggtgccggtcacccggcagtcgcggaagaactgccgc 816
||||||||||||||||||||||||||||||| | ||||| ||||||||||||||||
Sbjct: 292 gagttgccgaagatgaagtcgatggtgccggagatgacgcagttgcggaagaactgccgc 233
Query: 817 tgcgcgtgcgtgtacagcgtgtcctgg 843
| |||||| ||||||||||||||||
Sbjct: 232 cgggcgtgcacgtacagcgtgtcctgg 206
Score = 54.0 bits (27), Expect = 8e-006
Identities = 36/39 (92%)
Strand = Plus / Minus
Query: 451 gcgtagaagagcgtctcgaggccgaagtcgccgttccac 489
|||||| ||||||||| ||| ||||||||||||||||||
Sbjct: 595 gcgtagtagagcgtcttgagcccgaagtcgccgttccac 557
>gb|BJ465418.1|BJ465418 BJ465418 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags39m02 5', mRNA sequence
Length = 528
Score = 54.0 bits (27), Expect = 8e-006
Identities = 30/31 (96%)
Strand = Plus / Minus
Query: 756 ggagttgccgaagatgaagtcgatggtgccg 786
|||||||||||||| ||||||||||||||||
Sbjct: 393 ggagttgccgaagacgaagtcgatggtgccg 363
>gb|CK122193.1|CK122193 BES1824101p15 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010P151 5-PRIME, mRNA sequence
Length = 641
Score = 48.1 bits (24), Expect = 5e-004
Identities = 72/88 (81%)
Strand = Plus / Minus
Query: 757 gagttgccgaagatgaagtcgatggtgccggtcacccggcagtcgcggaagaactgccgc 816
|||||||||||||||||||| | ||||||| ||| |||| | | |||||||||||
Sbjct: 392 gagttgccgaagatgaagtccaccgtgccggccacgaggcaccgggtgtagaactgccgc 333
Query: 817 tgcgcgtgcgtgtacagcgtgtcctggt 844
||||||| |||||||||||||||||
Sbjct: 332 atggcgtgcgcgtacagcgtgtcctggt 305
>gb|CK122985.1|CK122985 BES1824101k13 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010K131 5-PRIME, mRNA sequence
Length = 611
Score = 48.1 bits (24), Expect = 5e-004
Identities = 72/88 (81%)
Strand = Plus / Minus
Query: 757 gagttgccgaagatgaagtcgatggtgccggtcacccggcagtcgcggaagaactgccgc 816
|||||||||||||||||||| | ||||||| ||| |||| | | |||||||||||
Sbjct: 392 gagttgccgaagatgaagtccaccgtgccggccacgaggcaccgggtgtagaactgccgc 333
Query: 817 tgcgcgtgcgtgtacagcgtgtcctggt 844
||||||| |||||||||||||||||
Sbjct: 332 atggcgtgcgcgtacagcgtgtcctggt 305
>gb|BG415761.2|BG415761 HVSMEk0007L15f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0007L15f, mRNA sequence
Length = 650
Score = 40.1 bits (20), Expect = 0.12
Identities = 29/32 (90%)
Strand = Plus / Minus
Query: 822 gtgcgtgtacagcgtgtcctggtagccgtcga 853
||||| ||| || |||||||||||||||||||
Sbjct: 478 gtgcgcgtagagggtgtcctggtagccgtcga 447
>gb|BM442638.2|BM442638 EBan01_SQ003_L22_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_L22 5', mRNA sequence
Length = 212
Score = 40.1 bits (20), Expect = 0.12
Identities = 23/24 (95%)
Strand = Plus / Minus
Query: 762 gccgaagatgaagtcgatggtgcc 785
||||||||||||||||||| ||||
Sbjct: 51 gccgaagatgaagtcgatgttgcc 28
>gb|CA029858.1|CA029858 HX05G15r HX Hordeum vulgare subsp. vulgare cDNA clone HX05G15
5-PRIME, mRNA sequence
Length = 638
Score = 40.1 bits (20), Expect = 0.12
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 759 gttgccgaagatgaagtcga 778
||||||||||||||||||||
Sbjct: 168 gttgccgaagatgaagtcga 149
>gb|BF619063.2|BF619063 HVSMEc0008J10f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0008J10f, mRNA sequence
Length = 820
Score = 38.2 bits (19), Expect = 0.47
Identities = 22/23 (95%)
Strand = Plus / Plus
Query: 317 ggatgaactgctgcccctggatg 339
||||||||| |||||||||||||
Sbjct: 736 ggatgaactactgcccctggatg 758
>gb|CV061819.1|CV061819 BNEL73c12 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL73c12 5' similar to No
Description, mRNA sequence
Length = 271
Score = 38.2 bits (19), Expect = 0.47
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 97 aaaagagaagagaaaaaag 115
|||||||||||||||||||
Sbjct: 122 aaaagagaagagaaaaaag 140
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 108,296
Number of Sequences: 312970
Number of extensions: 108296
Number of successful extensions: 32443
Number of sequences better than 0.5: 11
Number of HSP's better than 0.5 without gapping: 11
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 32417
Number of HSP's gapped (non-prelim): 26
length of query: 867
length of database: 175,134,539
effective HSP length: 19
effective length of query: 848
effective length of database: 169,188,109
effective search space: 143471516432
effective search space used: 143471516432
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)