BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3923331.2.1
(496 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AL500587.1|AL500587 AL500587 Hordeum vulgare Barke roots... 115 1e-024
gb|BM443222.2|BM443222 EBro02_SQ003_D03_R root, 3 week, hyd... 115 1e-024
gb|CA005005.1|CA005005 HU12K09u HU Hordeum vulgare subsp. v... 115 1e-024
gb|CA016096.1|CA016096 HV10C18u HV Hordeum vulgare subsp. v... 115 1e-024
gb|CA016336.1|CA016336 HV09G16u HV Hordeum vulgare subsp. v... 115 1e-024
gb|BM099511.2|BM099511 EBes01_SQ003_H09_R embryo sac, 4-6 D... 78 3e-013
>gb|AL500587.1|AL500587 AL500587 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW01C14u 3', mRNA sequence
Length = 526
Score = 115 bits (58), Expect = 1e-024
Identities = 133/158 (84%)
Strand = Plus / Plus
Query: 334 tcatccccacctctaatgctcgggggcctttcatccttccatacaggaacgtggttgagg 393
||||||||||||| ||| || | ||||||||||||||||||| |||||| || ||||||
Sbjct: 299 tcatccccacctcgaatacttgatggcctttcatccttccatataggaacatgcttgagg 358
Query: 394 gacctaaatttaccgagcactgcggattccaaggctcccagagttaactggcctgatctc 453
|| ||||||||| || |||| || || | ||| ||||| | ||||||||| |||||||
Sbjct: 359 gagctaaatttatcgtgcacagcagacttcaaagctcctaaagttaactgatctgatctt 418
Query: 454 atgtaaatgctctctgacttaaaccgccctctctctct 491
| |||||| |||||| |||||||||||||||||||||
Sbjct: 419 agataaatgttctctgtcttaaaccgccctctctctct 456
Score = 50.1 bits (25), Expect = 7e-005
Identities = 37/41 (90%)
Strand = Plus / Plus
Query: 191 gtagttacacaaaaattacttcaagctttgcacagggatgg 231
||||||| |||||||| || |||||||||||||| ||||||
Sbjct: 156 gtagttatacaaaaatcacctcaagctttgcacatggatgg 196
>gb|BM443222.2|BM443222 EBro02_SQ003_D03_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ003_D03 5', mRNA sequence
Length = 383
Score = 115 bits (58), Expect = 1e-024
Identities = 133/158 (84%)
Strand = Plus / Minus
Query: 334 tcatccccacctctaatgctcgggggcctttcatccttccatacaggaacgtggttgagg 393
||||||||||||| ||| || | ||||||||||||||||||| |||||| || ||||||
Sbjct: 352 tcatccccacctcgaatacttgatggcctttcatccttccatataggaacatgcttgagg 293
Query: 394 gacctaaatttaccgagcactgcggattccaaggctcccagagttaactggcctgatctc 453
|| ||||||||| || |||| || || | ||| ||||| | ||||||||| |||||||
Sbjct: 292 gagctaaatttatcgtgcacagcagacttcaaagctcctaaagttaactgatctgatctt 233
Query: 454 atgtaaatgctctctgacttaaaccgccctctctctct 491
| |||||| |||||| |||||||||||||||||||||
Sbjct: 232 agataaatgttctctgtcttaaaccgccctctctctct 195
>gb|CA005005.1|CA005005 HU12K09u HU Hordeum vulgare subsp. vulgare cDNA clone HU12K09
3-PRIME, mRNA sequence
Length = 543
Score = 115 bits (58), Expect = 1e-024
Identities = 133/158 (84%)
Strand = Plus / Plus
Query: 334 tcatccccacctctaatgctcgggggcctttcatccttccatacaggaacgtggttgagg 393
||||||||||||| ||| || | ||||||||||||||||||| |||||| || ||||||
Sbjct: 257 tcatccccacctcgaatacttgatggcctttcatccttccatataggaacatgcttgagg 316
Query: 394 gacctaaatttaccgagcactgcggattccaaggctcccagagttaactggcctgatctc 453
|| ||||||||| || |||| || || | ||| ||||| | ||||||||| |||||||
Sbjct: 317 gagctaaatttatcgtgcacagcagacttcaaagctcctaaagttaactgatctgatctt 376
Query: 454 atgtaaatgctctctgacttaaaccgccctctctctct 491
| |||||| |||||| |||||||||||||||||||||
Sbjct: 377 agataaatgttctctgtcttaaaccgccctctctctct 414
Score = 50.1 bits (25), Expect = 7e-005
Identities = 37/41 (90%)
Strand = Plus / Plus
Query: 191 gtagttacacaaaaattacttcaagctttgcacagggatgg 231
||||||| |||||||| || |||||||||||||| ||||||
Sbjct: 114 gtagttatacaaaaatcacctcaagctttgcacatggatgg 154
>gb|CA016096.1|CA016096 HV10C18u HV Hordeum vulgare subsp. vulgare cDNA clone HV10C18
3-PRIME, mRNA sequence
Length = 556
Score = 115 bits (58), Expect = 1e-024
Identities = 133/158 (84%)
Strand = Plus / Plus
Query: 334 tcatccccacctctaatgctcgggggcctttcatccttccatacaggaacgtggttgagg 393
||||||||||||| ||| || | ||||||||||||||||||| |||||| || ||||||
Sbjct: 258 tcatccccacctcgaatacttgatggcctttcatccttccatataggaacatgcttgagg 317
Query: 394 gacctaaatttaccgagcactgcggattccaaggctcccagagttaactggcctgatctc 453
|| ||||||||| || |||| || || | ||| ||||| | ||||||||| |||||||
Sbjct: 318 gagctaaatttatcgtgcacagcagacttcaaagctcctaaagttaactgatctgatctt 377
Query: 454 atgtaaatgctctctgacttaaaccgccctctctctct 491
| |||||| |||||| |||||||||||||||||||||
Sbjct: 378 agataaatgttctctgtcttaaaccgccctctctctct 415
Score = 50.1 bits (25), Expect = 7e-005
Identities = 37/41 (90%)
Strand = Plus / Plus
Query: 191 gtagttacacaaaaattacttcaagctttgcacagggatgg 231
||||||| |||||||| || |||||||||||||| ||||||
Sbjct: 115 gtagttatacaaaaatcacctcaagctttgcacatggatgg 155
>gb|CA016336.1|CA016336 HV09G16u HV Hordeum vulgare subsp. vulgare cDNA clone HV09G16
3-PRIME, mRNA sequence
Length = 540
Score = 115 bits (58), Expect = 1e-024
Identities = 133/158 (84%)
Strand = Plus / Plus
Query: 334 tcatccccacctctaatgctcgggggcctttcatccttccatacaggaacgtggttgagg 393
||||||||||||| ||| || | ||||||||||||||||||| |||||| || ||||||
Sbjct: 258 tcatccccacctcgaatacttgatggcctttcatccttccatataggaacatgcttgagg 317
Query: 394 gacctaaatttaccgagcactgcggattccaaggctcccagagttaactggcctgatctc 453
|| ||||||||| || |||| || || | ||| ||||| | ||||||||| |||||||
Sbjct: 318 gagctaaatttatcgtgcacagcagacttcaaagctcctaaagttaactgatctgatctt 377
Query: 454 atgtaaatgctctctgacttaaaccgccctctctctct 491
| |||||| |||||| |||||||||||||||||||||
Sbjct: 378 agataaatgttctctgtcttaaaccgccctctctctct 415
Score = 50.1 bits (25), Expect = 7e-005
Identities = 37/41 (90%)
Strand = Plus / Plus
Query: 191 gtagttacacaaaaattacttcaagctttgcacagggatgg 231
||||||| |||||||| || |||||||||||||| ||||||
Sbjct: 115 gtagttatacaaaaatcacctcaagctttgcacatggatgg 155
>gb|BM099511.2|BM099511 EBes01_SQ003_H09_R embryo sac, 4-6 DPA, no treatment, cv Optic,
EBes01 Hordeum vulgare subsp. vulgare cDNA clone
EBes01_SQ003_H09 5', mRNA sequence
Length = 689
Score = 77.8 bits (39), Expect = 3e-013
Identities = 99/119 (83%)
Strand = Plus / Minus
Query: 373 catacaggaacgtggttgagggacctaaatttaccgagcactgcggattccaaggctccc 432
|||| |||||| || |||||||| ||||||||| || |||| || || | ||| |||||
Sbjct: 689 catataggaacatgcttgagggagctaaatttatcgtgcacagcagacttcaaagctcct 630
Query: 433 agagttaactggcctgatctcatgtaaatgctctctgacttaaaccgccctctctctct 491
| ||||||||| ||||||| | |||||| |||||| |||||||||||||||||||||
Sbjct: 629 aaagttaactgatctgatcttagataaatgttctctgtcttaaaccgccctctctctct 571
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 49,048
Number of Sequences: 312970
Number of extensions: 49048
Number of successful extensions: 9475
Number of sequences better than 0.5: 6
Number of HSP's better than 0.5 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 9465
Number of HSP's gapped (non-prelim): 10
length of query: 496
length of database: 175,134,539
effective HSP length: 18
effective length of query: 478
effective length of database: 169,501,079
effective search space: 81021515762
effective search space used: 81021515762
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)