BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 3748389.2.1
         (663 letters)

Database: Hordeum_nucl_with_EST.fasta 
           312,970 sequences; 175,134,539 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|BQ768447.1|BQ768447  EBro08_SQ011_B19_R root, 3 week, dro...    38   0.36 
gb|CA014073.1|CA014073  HT10E24r HT Hordeum vulgare subsp. v...    38   0.36 

>gb|BQ768447.1|BQ768447 EBro08_SQ011_B19_R root, 3 week, drought-stressed, cv Optic, EBro08
           Hordeum vulgare subsp. vulgare cDNA clone
           EBro08_SQ011_B19 5', mRNA sequence
          Length = 418

 Score = 38.2 bits (19), Expect = 0.36
 Identities = 22/23 (95%)
 Strand = Plus / Plus

                                  
Query: 255 ctgacccacgccggctggaactc 277
           |||||||||||||| ||||||||
Sbjct: 174 ctgacccacgccgggtggaactc 196



>gb|CA014073.1|CA014073 HT10E24r HT Hordeum vulgare subsp. vulgare cDNA clone HT10E24
          5-PRIME, mRNA sequence
          Length = 641

 Score = 38.2 bits (19), Expect = 0.36
 Identities = 19/19 (100%)
 Strand = Plus / Minus

                             
Query: 32 ggcgaagctgacgagcgcg 50
          |||||||||||||||||||
Sbjct: 35 ggcgaagctgacgagcgcg 17



  Database: Hordeum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:16 PM
  Number of letters in database: 175,134,539
  Number of sequences in database:  312,970
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 67,559
Number of Sequences: 312970
Number of extensions: 67559
Number of successful extensions: 18510
Number of sequences better than  0.5: 2
Number of HSP's better than  0.5 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 18508
Number of HSP's gapped (non-prelim): 2
length of query: 663
length of database: 175,134,539
effective HSP length: 19
effective length of query: 644
effective length of database: 169,188,109
effective search space: 108957142196
effective search space used: 108957142196
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)