BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3713493.2.1
(696 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI960320.1|BI960320 HVSMEn0024D13f Hordeum vulgare rachi... 125 2e-027
gb|BI956408.1|BI956408 HVSMEn0003C22f Hordeum vulgare rachi... 117 5e-025
gb|BM376087.2|BM376087 EBma01_SQ002_E24_R maternal, 4 DPA, ... 117 5e-025
gb|BJ548121.1|BJ548121 BJ548121 K. Sato unpublished cDNA li... 117 5e-025
gb|BJ548884.1|BJ548884 BJ548884 K. Sato unpublished cDNA li... 117 5e-025
gb|AL512051.1|AL512051 AL512051 Hordeum vulgare Barke devel... 113 8e-024
gb|BE602701.1|BE602701 HVSMEh0101E04f Hordeum vulgare 5-45 ... 58 4e-007
gb|BE193568.2|BE193568 HVSMEh0081K21f Hordeum vulgare 5-45 ... 56 2e-006
gb|BI956401.1|BI956401 HVSMEn0003C12f Hordeum vulgare rachi... 54 6e-006
gb|AL502081.1|AL502081 AL502081 Hordeum vulgare Barke roots... 46 0.002
gb|AL502687.1|AL502687 AL502687 Hordeum vulgare Barke roots... 46 0.002
gb|AL508608.1|AL508608 AL508608 Hordeum vulgare Barke devel... 46 0.002
gb|BF256605.3|BF256605 HVSMEf0010H15f Hordeum vulgare seedl... 46 0.002
gb|BM100580.2|BM100580 EBma01_SQ005_K07_R maternal, 4 DPA, ... 46 0.002
gb|BQ759449.1|BQ759449 EBpi01_SQ004_E18_R pistil, 1 DPA, no... 46 0.002
gb|CA026139.1|CA026139 HZ55A05r HZ Hordeum vulgare subsp. v... 46 0.002
gb|CA027571.1|CA027571 HZ59F12r HZ Hordeum vulgare subsp. v... 46 0.002
gb|BG414821.1|BG414821 HVSMEk0003N14f Hordeum vulgare testa... 42 0.024
gb|AL500273.1|AL500273 AL500273 Hordeum vulgare Barke etiol... 40 0.095
gb|BJ452524.1|BJ452524 BJ452524 K. Sato unpublished cDNA li... 40 0.095
gb|BJ460071.1|BJ460071 BJ460071 K. Sato unpublished cDNA li... 40 0.095
gb|BQ458353.1|BQ458353 HA05H20r HA Hordeum vulgare subsp. v... 40 0.095
gb|BU966945.1|BU966945 HB02N12r BC Hordeum vulgare subsp. v... 40 0.095
gb|BU966991.1|BU966991 HB02P18r BC Hordeum vulgare subsp. v... 40 0.095
>gb|BI960320.1|BI960320 HVSMEn0024D13f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0024D13f, mRNA sequence
Length = 653
Score = 125 bits (63), Expect = 2e-027
Identities = 156/187 (83%)
Strand = Plus / Plus
Query: 480 cggccaggtcacggcaatggggctcgtgacgtcgtgcgcgccgtcgctccaggtgatgga 539
|||||| |||||||| |||||||||||||| | |||||||||||||||||| |||| |||
Sbjct: 129 cggccatgtcacggcgatggggctcgtgacattgtgcgcgccgtcgctccacgtgacgga 188
Query: 540 tccgaacgagtagctggaatccacgatcaccgggttgcctgacactgcgatggtgatttc 599
||||||||||| || || ||||||||||||||||| | ||| |||| |||||| ||
Sbjct: 189 cccgaacgagtacttgccgtcgacgatcaccgggttgccggccacggcgagggtgatctc 248
Query: 600 gtatcccaggctctgctgcttcccgtcgaacgcgagcttgctcggggtcaccgtgacatc 659
||| |||||||| | |||| | |||||||| |||||||| ||| |||||| || | ||
Sbjct: 249 gtacgccaggctccggtgctcctcgtcgaacacgagcttggccggcgtcaccttggcgtc 308
Query: 660 cacgccg 666
|||||||
Sbjct: 309 cacgccg 315
>gb|BI956408.1|BI956408 HVSMEn0003C22f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0003C22f, mRNA sequence
Length = 637
Score = 117 bits (59), Expect = 5e-025
Identities = 155/187 (82%)
Strand = Plus / Minus
Query: 480 cggccaggtcacggcaatggggctcgtgacgtcgtgcgcgccgtcgctccaggtgatgga 539
|||||| ||| |||| |||||||||||||| | |||||||||||||||||| |||| |||
Sbjct: 454 cggccatgtcccggcgatggggctcgtgacattgtgcgcgccgtcgctccacgtgacgga 395
Query: 540 tccgaacgagtagctggaatccacgatcaccgggttgcctgacactgcgatggtgatttc 599
||||||||||| || || ||||||||||||||||| | ||| |||| |||||| ||
Sbjct: 394 cccgaacgagtacttgccgtcgacgatcaccgggttgccggccacggcgagggtgatctc 335
Query: 600 gtatcccaggctctgctgcttcccgtcgaacgcgagcttgctcggggtcaccgtgacatc 659
||| |||||||| | |||| | |||||||| |||||||| ||| |||||| || | ||
Sbjct: 334 gtacgccaggctccggtgctcctcgtcgaacacgagcttggccggcgtcaccttggcgtc 275
Query: 660 cacgccg 666
|||||||
Sbjct: 274 cacgccg 268
>gb|BM376087.2|BM376087 EBma01_SQ002_E24_R maternal, 4 DPA, no treatment, cv Optic, EBma01
Hordeum vulgare subsp. vulgare cDNA clone
EBma01_SQ002_E24 5', mRNA sequence
Length = 436
Score = 117 bits (59), Expect = 5e-025
Identities = 155/187 (82%)
Strand = Plus / Minus
Query: 480 cggccaggtcacggcaatggggctcgtgacgtcgtgcgcgccgtcgctccaggtgatgga 539
|||||| |||||||| |||||||||||||| | |||| ||||||||||||| |||| |||
Sbjct: 304 cggccatgtcacggcgatggggctcgtgacattgtgcacgccgtcgctccacgtgacgga 245
Query: 540 tccgaacgagtagctggaatccacgatcaccgggttgcctgacactgcgatggtgatttc 599
||||||||||| || || ||||||||||||||||| | ||| |||| |||||| ||
Sbjct: 244 cccgaacgagtacttgccgtcgacgatcaccgggttgccggccacggcgagggtgatctc 185
Query: 600 gtatcccaggctctgctgcttcccgtcgaacgcgagcttgctcggggtcaccgtgacatc 659
||| |||||||| | |||| | |||||||| |||||||| ||| |||||| || | ||
Sbjct: 184 gtacgccaggctccggtgctcctcgtcgaacacgagcttggccggcgtcaccttggcgtc 125
Query: 660 cacgccg 666
|||||||
Sbjct: 124 cacgccg 118
>gb|BJ548121.1|BJ548121 BJ548121 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal19e21 3', mRNA sequence
Length = 584
Score = 117 bits (59), Expect = 5e-025
Identities = 155/187 (82%)
Strand = Plus / Plus
Query: 480 cggccaggtcacggcaatggggctcgtgacgtcgtgcgcgccgtcgctccaggtgatgga 539
|||||| |||||||| |||||||||||||| | |||| ||||||||||||| |||| |||
Sbjct: 279 cggccatgtcacggcgatggggctcgtgacattgtgcacgccgtcgctccacgtgacgga 338
Query: 540 tccgaacgagtagctggaatccacgatcaccgggttgcctgacactgcgatggtgatttc 599
||||||||||| || || ||||||||||||||||| | ||| |||| |||||| ||
Sbjct: 339 cccgaacgagtacttgccgtcgacgatcaccgggttgccggccacggcgagggtgatctc 398
Query: 600 gtatcccaggctctgctgcttcccgtcgaacgcgagcttgctcggggtcaccgtgacatc 659
||| |||||||| | |||| | |||||||| |||||||| ||| |||||| || | ||
Sbjct: 399 gtacgccaggctccggtgctcctcgtcgaacacgagcttggccggcgtcaccttggcgtc 458
Query: 660 cacgccg 666
|||||||
Sbjct: 459 cacgccg 465
>gb|BJ548884.1|BJ548884 BJ548884 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal37l10 3', mRNA sequence
Length = 523
Score = 117 bits (59), Expect = 5e-025
Identities = 155/187 (82%)
Strand = Plus / Plus
Query: 480 cggccaggtcacggcaatggggctcgtgacgtcgtgcgcgccgtcgctccaggtgatgga 539
|||||| |||||||| |||||||||||||| | |||| ||||||||||||| |||| |||
Sbjct: 276 cggccatgtcacggcgatggggctcgtgacattgtgcacgccgtcgctccacgtgacgga 335
Query: 540 tccgaacgagtagctggaatccacgatcaccgggttgcctgacactgcgatggtgatttc 599
||||||||||| || || ||||||||||||||||| | ||| |||| |||||| ||
Sbjct: 336 cccgaacgagtacttgccgtcgacgatcaccgggttgccggccacggcgagggtgatctc 395
Query: 600 gtatcccaggctctgctgcttcccgtcgaacgcgagcttgctcggggtcaccgtgacatc 659
||| |||||||| | |||| | |||||||| |||||||| ||| |||||| || | ||
Sbjct: 396 gtacgccaggctccggtgctcctcgtcgaacacgagcttggccggcgtcaccttggcgtc 455
Query: 660 cacgccg 666
|||||||
Sbjct: 456 cacgccg 462
>gb|AL512051.1|AL512051 AL512051 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY10D13u 3',
mRNA sequence
Length = 335
Score = 113 bits (57), Expect = 8e-024
Identities = 134/160 (83%)
Strand = Plus / Plus
Query: 480 cggccaggtcacggcaatggggctcgtgacgtcgtgcgcgccgtcgctccaggtgatgga 539
|||||| |||||||| |||||||||||||| | |||||||||||||||||| |||| |||
Sbjct: 164 cggccatgtcacggcgatggggctcgtgacattgtgcgcgccgtcgctccacgtgacgga 223
Query: 540 tccgaacgagtagctggaatccacgatcaccgggttgcctgacactgcgatggtgatttc 599
||||||||||| || || ||||||||||||||||| | ||| || | |||||| ||
Sbjct: 224 cccgaacgagtacttgccgtcgacgatcaccgggttgccggccacggcnagggtgatctc 283
Query: 600 gtatcccaggctctgctgcttcccgtcgaacgcgagcttg 639
||| |||||||| | |||| | |||||||| ||||||||
Sbjct: 284 gtacgccaggctccggtgctcctcgtcgaacacgagcttg 323
>gb|BE602701.1|BE602701 HVSMEh0101E04f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0101E04f, mRNA sequence
Length = 747
Score = 58.0 bits (29), Expect = 4e-007
Identities = 108/133 (81%), Gaps = 1/133 (0%)
Strand = Plus / Minus
Query: 519 gccgtcgctccaggtgatggatccgaacgagtagctggaatccacgatcacc-gggttgc 577
||||||||||||||||| ||| ||||||||||| | || ||||||||| |||||||
Sbjct: 559 gccgtcgctccaggtgacggaaccgaacgagtacttccggtcgacgatcaccggggttgc 500
Query: 578 ctgacactgcgatggtgatttcgtatcccaggctctgctgcttcccgtcgaacgcgagct 637
| | ||| |||| |||||| ||||| |||||||| | |||| | |||||||| |||| |
Sbjct: 499 cggccacggcgagggtgatctcgtacgccaggctccggtgctcctcgtcgaacacgagtt 440
Query: 638 tgctcggggtcac 650
||| ||| |||||
Sbjct: 439 tgcccggcgtcac 427
>gb|BE193568.2|BE193568 HVSMEh0081K21f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0081K21f, mRNA sequence
Length = 756
Score = 56.0 bits (28), Expect = 2e-006
Identities = 82/100 (82%)
Strand = Plus / Minus
Query: 567 caccgggttgcctgacactgcgatggtgatttcgtatcccaggctctgctgcttcccgtc 626
|||||||||||| | ||| |||| |||||| ||||| |||||||| | |||| | ||||
Sbjct: 466 caccgggttgccggccacggcgagggtgatctcgtacgccaggctccggtgctcctcgtc 407
Query: 627 gaacgcgagcttgctcggggtcaccgtgacatccacgccg 666
|||| |||||||| ||| |||||| || | |||||||||
Sbjct: 406 gaacacgagcttggccggcgtcaccttggcgtccacgccg 367
>gb|BI956401.1|BI956401 HVSMEn0003C12f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0003C12f, mRNA sequence
Length = 878
Score = 54.0 bits (27), Expect = 6e-006
Identities = 33/35 (94%)
Strand = Plus / Minus
Query: 496 atggggctcgtgacgtcgtgcgcgccgtcgctcca 530
|||||||||||||| | ||||||||||||||||||
Sbjct: 438 atggggctcgtgacattgtgcgcgccgtcgctcca 404
>gb|AL502081.1|AL502081 AL502081 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW06G19u 3', mRNA sequence
Length = 461
Score = 46.1 bits (23), Expect = 0.002
Identities = 44/51 (86%)
Strand = Plus / Plus
Query: 480 cggccaggtcacggcaatggggctcgtgacgtcgtgcgcgccgtcgctcca 530
||||||||| ||||| ||||||||||| || |||| |||||||||||||
Sbjct: 165 cggccaggtgacggcgatggggctcgtcaccaagtgctcgccgtcgctcca 215
>gb|AL502687.1|AL502687 AL502687 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW08G16u 3', mRNA sequence
Length = 652
Score = 46.1 bits (23), Expect = 0.002
Identities = 44/51 (86%)
Strand = Plus / Plus
Query: 480 cggccaggtcacggcaatggggctcgtgacgtcgtgcgcgccgtcgctcca 530
||||||||| ||||| ||||||||||| || |||| |||||||||||||
Sbjct: 165 cggccaggtgacggcgatggggctcgtcaccaagtgctcgccgtcgctcca 215
>gb|AL508608.1|AL508608 AL508608 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY09E08V 5',
mRNA sequence
Length = 583
Score = 46.1 bits (23), Expect = 0.002
Identities = 44/51 (86%)
Strand = Plus / Plus
Query: 480 cggccaggtcacggcaatggggctcgtgacgtcgtgcgcgccgtcgctcca 530
||||||||| ||||| ||||||||||| || |||| |||||||||||||
Sbjct: 173 cggccaggtgacggcgatggggctcgtcaccaagtgctcgccgtcgctcca 223
>gb|BF256605.3|BF256605 HVSMEf0010H15f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0010H15f, mRNA sequence
Length = 468
Score = 46.1 bits (23), Expect = 0.002
Identities = 44/51 (86%)
Strand = Plus / Minus
Query: 480 cggccaggtcacggcaatggggctcgtgacgtcgtgcgcgccgtcgctcca 530
||||||||| ||||| ||||||||||| || |||| |||||||||||||
Sbjct: 291 cggccaggtgacggcgatggggctcgtcaccgagtgctcgccgtcgctcca 241
>gb|BM100580.2|BM100580 EBma01_SQ005_K07_R maternal, 4 DPA, no treatment, cv Optic, EBma01
Hordeum vulgare subsp. vulgare cDNA clone
EBma01_SQ005_K07 5', mRNA sequence
Length = 440
Score = 46.1 bits (23), Expect = 0.002
Identities = 44/51 (86%)
Strand = Plus / Minus
Query: 480 cggccaggtcacggcaatggggctcgtgacgtcgtgcgcgccgtcgctcca 530
||||||||| ||||| ||||||||||| || |||| |||||||||||||
Sbjct: 262 cggccaggtgacggcgatggggctcgtcaccgagtgctcgccgtcgctcca 212
>gb|BQ759449.1|BQ759449 EBpi01_SQ004_E18_R pistil, 1 DPA, no treatment, cv Optic, EBpi01
Hordeum vulgare subsp. vulgare cDNA clone
EBpi01_SQ004_E18 5', mRNA sequence
Length = 349
Score = 46.1 bits (23), Expect = 0.002
Identities = 44/51 (86%)
Strand = Plus / Minus
Query: 480 cggccaggtcacggcaatggggctcgtgacgtcgtgcgcgccgtcgctcca 530
||||||||| ||||| ||||||||||| || |||| |||||||||||||
Sbjct: 82 cggccaggtgacggcgatggggctcgtcaccgagtgctcgccgtcgctcca 32
>gb|CA026139.1|CA026139 HZ55A05r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ55A05
5-PRIME, mRNA sequence
Length = 428
Score = 46.1 bits (23), Expect = 0.002
Identities = 44/51 (86%)
Strand = Plus / Minus
Query: 480 cggccaggtcacggcaatggggctcgtgacgtcgtgcgcgccgtcgctcca 530
||||||||| ||||| ||||||||||| || |||| |||||||||||||
Sbjct: 275 cggccaggtgacggcgatggggctcgtcaccaagtgctcgccgtcgctcca 225
>gb|CA027571.1|CA027571 HZ59F12r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ59F12
5-PRIME, mRNA sequence
Length = 422
Score = 46.1 bits (23), Expect = 0.002
Identities = 44/51 (86%)
Strand = Plus / Minus
Query: 480 cggccaggtcacggcaatggggctcgtgacgtcgtgcgcgccgtcgctcca 530
||||||||| ||||| ||||||||||| || |||| |||||||||||||
Sbjct: 269 cggccaggtgacggcgatggggctcgtcaccaagtgctcgccgtcgctcca 219
>gb|BG414821.1|BG414821 HVSMEk0003N14f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0003N14f, mRNA sequence
Length = 847
Score = 42.1 bits (21), Expect = 0.024
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 467 ctccaccgttggacggccagg 487
|||||||||||||||||||||
Sbjct: 157 ctccaccgttggacggccagg 177
>gb|AL500273.1|AL500273 AL500273 Hordeum vulgare Barke etiolated leaves Hordeum vulgare
subsp. vulgare cDNA clone HK05N02r 3', mRNA sequence
Length = 418
Score = 40.1 bits (20), Expect = 0.095
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 497 tggggctcgtgacgtcgtgc 516
||||||||||||||||||||
Sbjct: 416 tggggctcgtgacgtcgtgc 397
>gb|BJ452524.1|BJ452524 BJ452524 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak39f10 5', mRNA sequence
Length = 631
Score = 40.1 bits (20), Expect = 0.095
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 649 accgtgacatccacgccgta 668
||||||||||||||||||||
Sbjct: 167 accgtgacatccacgccgta 148
>gb|BJ460071.1|BJ460071 BJ460071 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak39f10 3', mRNA sequence
Length = 600
Score = 40.1 bits (20), Expect = 0.095
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 649 accgtgacatccacgccgta 668
||||||||||||||||||||
Sbjct: 433 accgtgacatccacgccgta 452
>gb|BQ458353.1|BQ458353 HA05H20r HA Hordeum vulgare subsp. vulgare cDNA clone HA05H20
5-PRIME, mRNA sequence
Length = 556
Score = 40.1 bits (20), Expect = 0.095
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 649 accgtgacatccacgccgta 668
||||||||||||||||||||
Sbjct: 158 accgtgacatccacgccgta 139
>gb|BU966945.1|BU966945 HB02N12r BC Hordeum vulgare subsp. vulgare cDNA clone HB02N12
5-PRIME, mRNA sequence
Length = 540
Score = 40.1 bits (20), Expect = 0.095
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 649 accgtgacatccacgccgta 668
||||||||||||||||||||
Sbjct: 194 accgtgacatccacgccgta 175
>gb|BU966991.1|BU966991 HB02P18r BC Hordeum vulgare subsp. vulgare cDNA clone HB02P18
5-PRIME, mRNA sequence
Length = 512
Score = 40.1 bits (20), Expect = 0.095
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 649 accgtgacatccacgccgta 668
||||||||||||||||||||
Sbjct: 196 accgtgacatccacgccgta 177
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 58,672
Number of Sequences: 312970
Number of extensions: 58672
Number of successful extensions: 16096
Number of sequences better than 0.5: 24
Number of HSP's better than 0.5 without gapping: 24
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 16062
Number of HSP's gapped (non-prelim): 28
length of query: 696
length of database: 175,134,539
effective HSP length: 19
effective length of query: 677
effective length of database: 169,188,109
effective search space: 114540349793
effective search space used: 114540349793
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)