BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3670899.2.1
(555 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AL503069.1|AL503069 AL503069 Hordeum vulgare Barke roots... 414 e-114
gb|BQ768402.1|BQ768402 EBro08_SQ011_I17_R root, 3 week, dro... 176 5e-043
gb|CB877731.1|CB877731 HP05P15T HP Hordeum vulgare subsp. v... 131 3e-029
gb|BJ455732.1|BJ455732 BJ455732 K. Sato unpublished cDNA li... 38 0.30
gb|BM442252.2|BM442252 EBan01_SQ002_K15_R anther, yellow st... 38 0.30
gb|BM099614.2|BM099614 EBes01_SQ003_L14_R embryo sac, 4-6 D... 38 0.30
>gb|AL503069.1|AL503069 AL503069 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW09J19u 3', mRNA sequence
Length = 562
Score = 414 bits (209), Expect = e-114
Identities = 335/376 (89%), Gaps = 1/376 (0%)
Strand = Plus / Plus
Query: 137 ttagctatcgaacaacttgaggccgaagccgacgtcctcgcagtgccggacgtacggctt 196
|||||||||||| | |||||| ||||| |||||||||||||||||||||||||| |||||
Sbjct: 179 ttagctatcgaagagcttgagaccgaacccgacgtcctcgcagtgccggacgtagggctt 238
Query: 197 gacagcggcgacgtcgacgtccgtcttggccctgcacccgaggatcggcggcgagtgcag 256
||| ||| ||||| || ||| ||||||||| ||| ||||| |||||||||||||||||
Sbjct: 239 gacgcgggccacgtccacctccttcttggcccggcagccgagtatcggcggcgagtgcag 298
Query: 257 gcacggctccacggacgtggaccggacgcacgcgacgtcggaggtctccctgttccagtc 316
||||||||| ||||||||||||||||||||||| || | || |||| ||| ||||||
Sbjct: 299 gcacggctcgacggacgtggaccggacgcacgccacctgcgacacctccttgtcccagtc 358
Query: 317 cggccggatcaggatccacggcttcacgccggcgaagctgtacgcgacgtagccgaaggt 376
|||||||| ||||||||||||||| ||||||||||||| |||||||||||| ||||| ||
Sbjct: 359 cggccggagcaggatccacggcttgacgccggcgaagccgtacgcgacgtacccgaacgt 418
Query: 377 ggaccaggcgctcatcgtgatcttgtcgcagtagctgagcaggtagatctcggccagcgc 436
||||||||||||||| | ||||||||||||||||||||||||||||||||| ||||||||
Sbjct: 419 ggaccaggcgctcatggcgatcttgtcgcagtagctgagcaggtagatctccgccagcgc 478
Query: 437 cttctggttgtggtcgttggacgtgtactgctgctgctcctcgtggct-gggctggaaca 495
|||||||||||| |||||||||| ||||| ||||| |||||||||||| ||| ||| |||
Sbjct: 479 cttctggttgtgctcgttggacgcgtactcctgcttctcctcgtggctcgggntggtaca 538
Query: 496 ccgccacgatctcccc 511
||||||||| ||||||
Sbjct: 539 ccgccacgacctcccc 554
>gb|BQ768402.1|BQ768402 EBro08_SQ011_I17_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ011_I17 5', mRNA sequence
Length = 430
Score = 176 bits (89), Expect = 5e-043
Identities = 137/153 (89%)
Strand = Plus / Minus
Query: 137 ttagctatcgaacaacttgaggccgaagccgacgtcctcgcagtgccggacgtacggctt 196
|||||||||||| | |||||| ||||| |||||||||||||||||||||||||| |||||
Sbjct: 161 ttagctatcgaagagcttgagaccgaacccgacgtcctcgcagtgccggacgtagggctt 102
Query: 197 gacagcggcgacgtcgacgtccgtcttggccctgcacccgaggatcggcggcgagtgcag 256
||| ||| ||||| || ||| ||||||||| ||| ||||| |||||||||||||||||
Sbjct: 101 gacgcgggccacgtccacctccttcttggcccggcagccgagtatcggcggcgagtgcag 42
Query: 257 gcacggctccacggacgtggaccggacgcacgc 289
||||||||| |||||||||||||||||||||||
Sbjct: 41 gcacggctcgacggacgtggaccggacgcacgc 9
>gb|CB877731.1|CB877731 HP05P15T HP Hordeum vulgare subsp. vulgare cDNA clone HP05P15
5-PRIME, mRNA sequence
Length = 142
Score = 131 bits (66), Expect = 3e-029
Identities = 117/134 (87%)
Strand = Plus / Minus
Query: 137 ttagctatcgaacaacttgaggccgaagccgacgtcctcgcagtgccggacgtacggctt 196
|||||||||||| | |||||| ||||| |||||||||||||||||||||||||| |||||
Sbjct: 134 ttagctatcgaagagcttgagaccgaacccgacgtcctcgcagtgccggacgtagggctt 75
Query: 197 gacagcggcgacgtcgacgtccgtcttggccctgcacccgaggatcggcggcgagtgcag 256
||| ||| ||||| || ||| | ||||||| ||| ||||| |||||||||||||||||
Sbjct: 74 gacgcgggccacgtccacctccttgttggcccggcagccgagtatcggcggcgagtgcag 15
Query: 257 gcacggctccacgg 270
||||||||| ||||
Sbjct: 14 gcacggctcgacgg 1
>gb|BJ455732.1|BJ455732 BJ455732 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak18a01 3', mRNA sequence
Length = 607
Score = 38.2 bits (19), Expect = 0.30
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 90 agttcagaaaagagttgga 108
|||||||||||||||||||
Sbjct: 588 agttcagaaaagagttgga 570
>gb|BM442252.2|BM442252 EBan01_SQ002_K15_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ002_K15 5', mRNA sequence
Length = 442
Score = 38.2 bits (19), Expect = 0.30
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 90 agttcagaaaagagttgga 108
|||||||||||||||||||
Sbjct: 303 agttcagaaaagagttgga 321
>gb|BM099614.2|BM099614 EBes01_SQ003_L14_R embryo sac, 4-6 DPA, no treatment, cv Optic,
EBes01 Hordeum vulgare subsp. vulgare cDNA clone
EBes01_SQ003_L14 5', mRNA sequence
Length = 605
Score = 38.2 bits (19), Expect = 0.30
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 90 agttcagaaaagagttgga 108
|||||||||||||||||||
Sbjct: 104 agttcagaaaagagttgga 122
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 69,073
Number of Sequences: 312970
Number of extensions: 69073
Number of successful extensions: 17210
Number of sequences better than 0.5: 6
Number of HSP's better than 0.5 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 17202
Number of HSP's gapped (non-prelim): 7
length of query: 555
length of database: 175,134,539
effective HSP length: 19
effective length of query: 536
effective length of database: 169,188,109
effective search space: 90684826424
effective search space used: 90684826424
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)