BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3665525.2.1
(620 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG416042.1|BG416042 HVSMEk0009I22f Hordeum vulgare testa... 281 1e-074
gb|BG414688.2|BG414688 HVSMEk0003F21f Hordeum vulgare testa... 258 2e-067
gb|CK122677.1|CK122677 BES1824105a18 BES1824 Hordeum vulgar... 176 6e-043
>gb|BG416042.1|BG416042 HVSMEk0009I22f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0009I22f, mRNA sequence
Length = 1066
Score = 281 bits (142), Expect = 1e-074
Identities = 299/346 (86%), Gaps = 4/346 (1%)
Strand = Plus / Minus
Query: 278 aagctctagcatttttacctctggg-tggcatagccctgagcattttggaatgtcccaaa 336
|||||| ||||| ||||||| |||| |||||||||||||| |||||||| ||||||||
Sbjct: 369 aagctccagcatctttacctttggggtggcatagccctgaacattttgggatgtcccagt 310
Query: 337 cagcaactgaagactccgt-gcagtcaggttcacagagcgcatgtgtgttctcatggttg 395
| |||| || || ||||| ||| || || || || || ||||| | |||||| ||||||
Sbjct: 309 cggcaatcga-gaatccgttgcactcgggctcgcaaagggcatgggggttctcgtggttg 251
Query: 396 acgttcatagcaagcatccacgatccaattgt-aacgtcctagatactaaacatacggaa 454
|||||||||||||||||||| |||||||| || |||||||| | | | ||||| |||||
Sbjct: 250 acgttcatagcaagcatccaggatccaatggttaacgtcctcgttgttgaacattcggaa 191
Query: 455 actgttgttcctcaaagcaaccaagctagccaccacgtcagctgataaagcataaattgg 514
||||||||| |||| |||||||||||| |||||||| |||||||||||||||||||| ||
Sbjct: 190 actgttgtttctcagagcaaccaagcttgccaccacatcagctgataaagcataaatagg 131
Query: 515 cccatatgcatgaagaaagtattctgatccgagtaagaaggattggggctcataccattt 574
||||||||||||||| |||||||||||||| |||| |||||| || |||||| |||||||
Sbjct: 130 cccatatgcatgaaggaagtattctgatccaagtaggaaggactgtggctcacaccattt 71
Query: 575 caacttaggatcagtaaaaacaggccccttcttcatgcatccaatg 620
||| || |||||||| ||||||||||| ||||||||||||||||||
Sbjct: 70 caatttgggatcagtgaaaacaggccctttcttcatgcatccaatg 25
>gb|BG414688.2|BG414688 HVSMEk0003F21f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0003F21f, mRNA sequence
Length = 625
Score = 258 bits (130), Expect = 2e-067
Identities = 223/254 (87%)
Strand = Plus / Minus
Query: 276 tgaagctctagcatttttacctctgggtggcatagccctgagcattttggaatgtcccaa 335
|||||||| ||||| |||||||||||||||||||||||||| |||||||| ||||||||
Sbjct: 256 tgaagctccagcatctttacctctgggtggcatagccctgaacattttgggatgtcccag 197
Query: 336 acagcaactgaagactccgtgcagtcaggttcacagagcgcatgtgtgttctcatggttg 395
|| |||| || || ||||||| || || || ||||| ||||| |||||||| ||||||
Sbjct: 196 acggcaatcgaggatgccgtgcactcgggctcgcagagggcatgggtgttctcgtggttg 137
Query: 396 acgttcatagcaagcatccacgatccaattgtaacgtcctagatactaaacatacggaaa 455
|||||||||||||||||||| |||||||| |||||||||| | | | ||||| ||||||
Sbjct: 136 acgttcatagcaagcatccaggatccaatggtaacgtcctcgttgttgaacattcggaaa 77
Query: 456 ctgttgttcctcaaagcaaccaagctagccaccacgtcagctgataaagcataaattggc 515
|||||||| |||| |||||||||||| |||||||| |||||||||||||||||||| |||
Sbjct: 76 ctgttgtttctcagagcaaccaagcttgccaccacatcagctgataaagcataaataggc 17
Query: 516 ccatatgcatgaag 529
||||||||||||||
Sbjct: 16 ccatatgcatgaag 3
>gb|CK122677.1|CK122677 BES1824105a18 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010A185 5-PRIME, mRNA sequence
Length = 627
Score = 176 bits (89), Expect = 6e-043
Identities = 161/185 (87%)
Strand = Plus / Minus
Query: 276 tgaagctctagcatttttacctctgggtggcatagccctgagcattttggaatgtcccaa 335
|||||||| ||||| |||||||||||||||||||||||||| |||||||| ||||||||
Sbjct: 185 tgaagctccagcatctttacctctgggtggcatagccctgaacattttgggatgtcccag 126
Query: 336 acagcaactgaagactccgtgcagtcaggttcacagagcgcatgtgtgttctcatggttg 395
|| |||| || || ||||||| || ||||| ||||| ||||| |||||||||||||||
Sbjct: 125 acggcaatcgaggatgccgtgcactcgggttcgcagagggcatgggtgttctcatggttg 66
Query: 396 acgttcatagcaagcatccacgatccaattgtaacgtcctagatactaaacatacggaaa 455
|||||||||||||||||||| |||||||| |||||||||| | | | ||||| ||||||
Sbjct: 65 acgttcatagcaagcatccaggatccaatggtaacgtcctcgttgttgaacattcggaaa 6
Query: 456 ctgtt 460
|||||
Sbjct: 5 ctgtt 1
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 46,688
Number of Sequences: 312970
Number of extensions: 46688
Number of successful extensions: 13112
Number of sequences better than 0.5: 3
Number of HSP's better than 0.5 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 13104
Number of HSP's gapped (non-prelim): 5
length of query: 620
length of database: 175,134,539
effective HSP length: 19
effective length of query: 601
effective length of database: 169,188,109
effective search space: 101682053509
effective search space used: 101682053509
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)