BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3390529.2.1
(407 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF624193.2|BF624193 HVSMEa0012J04f Hordeum vulgare seedl... 363 3e-099
gb|BG300284.1|BG300284 HVSMEb0011P06f Hordeum vulgare seedl... 363 3e-099
gb|CA024561.1|CA024561 HZ49K12r HZ Hordeum vulgare subsp. v... 319 4e-086
gb|BF266997.3|BF266997 HV_CEa0016K10f Hordeum vulgare seedl... 293 2e-078
gb|BM375220.2|BM375220 EBem06_SQ002_L10_R embryo, 21 DPA, n... 276 5e-073
gb|BU995376.1|BU995376 HM10B01r HM Hordeum vulgare subsp. v... 218 1e-055
gb|CB881502.1|CB881502 HM10A02w HM Hordeum vulgare subsp. v... 167 3e-040
gb|AF474923.1| Hordeum vulgare subsp. vulgare chalcone isom... 151 2e-035
gb|BI957452.1|BI957452 HVSMEn0009K07f Hordeum vulgare rachi... 100 7e-020
gb|BI956899.1|BI956899 HVSMEn0005O19f Hordeum vulgare rachi... 94 4e-018
gb|CX662506.1|CX662506 EST 322 Expressed sequences from Bar... 82 2e-014
gb|CX662646.1|CX662646 EST 477 Expressed sequences from Bar... 82 2e-014
emb|AJ507395.1|HVU507395 Hordeum vulgare subsp. vulgare rpo... 42 0.014
emb|AJ507396.1|HVU507396 Hordeum vulgare subsp. vulgare mRN... 42 0.014
gb|BQ762901.1|BQ762901 EBro02_SQ006_P10_R root, 3 week, hyd... 40 0.054
gb|BM378047.2|BM378047 EBro08_SQ004_O21_R root, 3 week, dro... 38 0.22
gb|CA022183.1|CA022183 HZ42G07r HZ Hordeum vulgare subsp. v... 38 0.22
>gb|BF624193.2|BF624193 HVSMEa0012J04f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0012J04f, mRNA sequence
Length = 487
Score = 363 bits (183), Expect = 3e-099
Identities = 314/357 (87%), Gaps = 3/357 (0%)
Strand = Plus / Plus
Query: 54 catggccgtgccggaggtggtggtcgacggcgtcgtcttcccgccggtggcccgcccgcc 113
|||||||||| ||||| ||| ||||||||||||||||||||||||| | |||||||||||
Sbjct: 16 catggccgtgtcggagctggaggtcgacggcgtcgtcttcccgccgctcgcccgcccgcc 75
Query: 114 gggctccgccggctcgcacttcctcggcggcgcaggcgtgcgaggcctcgagatcggagg 173
|||||||||| | | |||||||||| ||||||||||||||| || | |||||||| ||
Sbjct: 76 gggctccgcccacgcccacttcctcgccggcgcaggcgtgcgcgggatggagatcggcgg 135
Query: 174 caacttcatcaagttcacggccatcggcgtgtacctggagg---acgcggccgtgcccgc 230
| ||||||||||||||||||||||||||||||||||| ||| |||| ||||| ||||
Sbjct: 136 ccacttcatcaagttcacggccatcggcgtgtacctgcaggccgacgccgccgtctccgc 195
Query: 231 gctggccaagaagtggggcggcaagacggccgacgagctcgcctccgacgccgccttctt 290
||| ||| ||||||| ||||||| | |||| ||| |||||||||||||||||||||||
Sbjct: 196 gctcgccgccaagtgggccggcaagcccgccgccgatctcgcctccgacgccgccttctt 255
Query: 291 ccgcgacgtcgtcacgggcgacttcgagaagttcacgagggtgacgatgatcctcccgct 350
||||||||||||||| ||||| ||||||||||||||||||||||| |||||||| |||||
Sbjct: 256 ccgcgacgtcgtcaccggcgagttcgagaagttcacgagggtgacaatgatcctgccgct 315
Query: 351 gacgggcgagcagtacgcggagaaagtgacggagaactgcgtggcgttctggaaggc 407
|||||||| ||||||| |||| || |||||||||||||| || |||| |||||||||
Sbjct: 316 gacgggcgcgcagtactcggacaaggtgacggagaactgtgtcgcgtactggaaggc 372
>gb|BG300284.1|BG300284 HVSMEb0011P06f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0011P06f, mRNA sequence
Length = 536
Score = 363 bits (183), Expect = 3e-099
Identities = 314/357 (87%), Gaps = 3/357 (0%)
Strand = Plus / Plus
Query: 54 catggccgtgccggaggtggtggtcgacggcgtcgtcttcccgccggtggcccgcccgcc 113
|||||||||| ||||| ||| ||||||||||||||||||||||||| | |||||||||||
Sbjct: 82 catggccgtgtcggagctggaggtcgacggcgtcgtcttcccgccgctcgcccgcccgcc 141
Query: 114 gggctccgccggctcgcacttcctcggcggcgcaggcgtgcgaggcctcgagatcggagg 173
|||||||||| | | |||||||||| ||||||||||||||| || | |||||||| ||
Sbjct: 142 gggctccgcccacgcccacttcctcgccggcgcaggcgtgcgcgggatggagatcggcgg 201
Query: 174 caacttcatcaagttcacggccatcggcgtgtacctggagg---acgcggccgtgcccgc 230
| ||||||||||||||||||||||||||||||||||| ||| |||| ||||| ||||
Sbjct: 202 ccacttcatcaagttcacggccatcggcgtgtacctgcaggccgacgccgccgtctccgc 261
Query: 231 gctggccaagaagtggggcggcaagacggccgacgagctcgcctccgacgccgccttctt 290
||| ||| ||||||| ||||||| | |||| ||| |||||||||||||||||||||||
Sbjct: 262 gctcgccgccaagtgggccggcaagcccgccgccgatctcgcctccgacgccgccttctt 321
Query: 291 ccgcgacgtcgtcacgggcgacttcgagaagttcacgagggtgacgatgatcctcccgct 350
||||||||||||||| ||||| ||||||||||||||||||||||| |||||||| |||||
Sbjct: 322 ccgcgacgtcgtcaccggcgagttcgagaagttcacgagggtgacaatgatcctgccgct 381
Query: 351 gacgggcgagcagtacgcggagaaagtgacggagaactgcgtggcgttctggaaggc 407
|||||||| ||||||| |||| || |||||||||||||| || |||| |||||||||
Sbjct: 382 gacgggcgcgcagtactcggacaaggtgacggagaactgtgtcgcgtactggaaggc 438
>gb|CA024561.1|CA024561 HZ49K12r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ49K12
5-PRIME, mRNA sequence
Length = 499
Score = 319 bits (161), Expect = 4e-086
Identities = 268/303 (88%), Gaps = 3/303 (0%)
Strand = Plus / Plus
Query: 108 cccgccgggctccgccggctcgcacttcctcggcggcgcaggcgtgcgaggcctcgagat 167
|||||||||||||||| | | |||||||||| ||||||||||||||| || | |||||
Sbjct: 1 cccgccgggctccgcccacgcccacttcctcgccggcgcaggcgtgcgcgggatggagat 60
Query: 168 cggaggcaacttcatcaagttcacggccatcggcgtgtacctggagg---acgcggccgt 224
||| ||||||||||||||||||||||||||||||||||||||| ||| |||| |||||
Sbjct: 61 cggcggcaacttcatcaagttcacggccatcggcgtgtacctgcaggccgacgccgccgt 120
Query: 225 gcccgcgctggccaagaagtggggcggcaagacggccgacgagctcgcctccgacgccgc 284
||||||| ||| ||||||| ||||||| | |||| ||| |||||||||||||||||
Sbjct: 121 ctccgcgctcgccgccaagtgggccggcaagcccgccgccgatctcgcctccgacgccgc 180
Query: 285 cttcttccgcgacgtcgtcacgggcgacttcgagaagttcacgagggtgacgatgatcct 344
||||||||||||||||||||| ||||| ||||||||||||||||||||||||||||||||
Sbjct: 181 cttcttccgcgacgtcgtcaccggcgagttcgagaagttcacgagggtgacgatgatcct 240
Query: 345 cccgctgacgggcgagcagtacgcggagaaagtgacggagaactgcgtggcgttctggaa 404
||||||||||||| ||||||| |||| || ||||||||||||||||| |||| ||||||
Sbjct: 241 gccgctgacgggcgcgcagtactcggacaaggtgacggagaactgcgtcgcgtactggaa 300
Query: 405 ggc 407
|||
Sbjct: 301 ggc 303
>gb|BF266997.3|BF266997 HV_CEa0016K10f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0016K10f, mRNA sequence
Length = 751
Score = 293 bits (148), Expect = 2e-078
Identities = 261/298 (87%), Gaps = 3/298 (1%)
Strand = Plus / Plus
Query: 113 cgggctccgccggctcgcacttcctcggcggcgcaggcgtgcgaggcctcgagatcggag 172
||||||||||| | | |||||||||| ||||||||||||||| || | |||||||| |
Sbjct: 1 cgggctccgcccacgcccacttcctcgccggcgcaggcgtgcgcgggatggagatcggcg 60
Query: 173 gcaacttcatcaagttcacggccatcggcgtgtacctggagg---acgcggccgtgcccg 229
|| ||||||||||||||||||||||||||||||||||| ||| |||| ||||| |||
Sbjct: 61 gccacttcatcaagttcacggccatcggcgtgtacctgcaggccgacgccgccgtctccg 120
Query: 230 cgctggccaagaagtggggcggcaagacggccgacgagctcgcctccgacgccgccttct 289
|||| ||| ||||||| ||||||| | |||| ||| ||||||||||||||||||||||
Sbjct: 121 cgctcgccgccaagtgggccggcaagcccgccgccgatctcgcctccgacgccgccttct 180
Query: 290 tccgcgacgtcgtcacgggcgacttcgagaagttcacgagggtgacgatgatcctcccgc 349
|||||||||||||||| ||||| |||||||||||||||||||||||||||||||| ||||
Sbjct: 181 tccgcgacgtcgtcaccggcgagttcgagaagttcacgagggtgacgatgatcctgccgc 240
Query: 350 tgacgggcgagcagtacgcggagaaagtgacggagaactgcgtggcgttctggaaggc 407
||||||||| ||||||| |||| || |||||||||||||| || |||| |||||||||
Sbjct: 241 tgacgggcgcgcagtactcggacaaggtgacggagaactgtgtcgcgtactggaaggc 298
>gb|BM375220.2|BM375220 EBem06_SQ002_L10_R embryo, 21 DPA, no treatment, cv Optic, EBem06
Hordeum vulgare subsp. vulgare cDNA clone
EBem06_SQ002_L10 5', mRNA sequence
Length = 375
Score = 276 bits (139), Expect = 5e-073
Identities = 240/273 (87%), Gaps = 3/273 (1%)
Strand = Plus / Plus
Query: 54 catggccgtgccggaggtggtggtcgacggcgtcgtcttcccgccggtggcccgcccgcc 113
|||||||||| ||||| ||| ||||||||||||||||||||||||| | |||||||||||
Sbjct: 103 catggccgtgtcggagctggaggtcgacggcgtcgtcttcccgccgctcgcccgcccgcc 162
Query: 114 gggctccgccggctcgcacttcctcggcggcgcaggcgtgcgaggcctcgagatcggagg 173
|||||||||| | | |||||||||| ||||||||||||||| || | |||||||| ||
Sbjct: 163 gggctccgcccacgcccacttcctcgccggcgcaggcgtgcgcgggatggagatcggcgg 222
Query: 174 caacttcatcaagttcacggccatcggcgtgtacctggag---gacgcggccgtgcccgc 230
||||||||||||||||||||||||||||||||||||| || ||||| ||||| ||||
Sbjct: 223 caacttcatcaagttcacggccatcggcgtgtacctgcaggccgacgccgccgtctccgc 282
Query: 231 gctggccaagaagtggggcggcaagacggccgacgagctcgcctccgacgccgccttctt 290
||| ||| ||||||| ||||||| | |||| ||| |||||||||||||||||||||||
Sbjct: 283 gctcgccgccaagtgggccggcaagcccgccgccgatctcgcctccgacgccgccttctt 342
Query: 291 ccgcgacgtcgtcacgggcgacttcgagaagtt 323
||||||||||||||| ||||| |||||||||||
Sbjct: 343 ccgcgacgtcgtcaccggcgagttcgagaagtt 375
>gb|BU995376.1|BU995376 HM10B01r HM Hordeum vulgare subsp. vulgare cDNA clone HM10B01
5-PRIME, mRNA sequence
Length = 602
Score = 218 bits (110), Expect = 1e-055
Identities = 152/166 (91%)
Strand = Plus / Plus
Query: 242 agtggggcggcaagacggccgacgagctcgcctccgacgccgccttcttccgcgacgtcg 301
|||||| ||||||| | |||| ||| ||||||||||||||||||||||||||||||||||
Sbjct: 37 agtgggccggcaagcccgccgccgatctcgcctccgacgccgccttcttccgcgacgtcg 96
Query: 302 tcacgggcgacttcgagaagttcacgagggtgacgatgatcctcccgctgacgggcgagc 361
|||| ||||| |||||||||||||||||||||||||||||||| ||||||||||||| ||
Sbjct: 97 tcaccggcgagttcgagaagttcacgagggtgacgatgatcctgccgctgacgggcgcgc 156
Query: 362 agtacgcggagaaagtgacggagaactgcgtggcgttctggaaggc 407
||||| |||| || ||||||||||||||||| |||| |||||||||
Sbjct: 157 agtactcggacaaggtgacggagaactgcgtcgcgtactggaaggc 202
>gb|CB881502.1|CB881502 HM10A02w HM Hordeum vulgare subsp. vulgare cDNA clone HM10A02
3-PRIME, mRNA sequence
Length = 630
Score = 167 bits (84), Expect = 3e-040
Identities = 111/120 (92%)
Strand = Plus / Minus
Query: 288 cttccgcgacgtcgtcacgggcgacttcgagaagttcacgagggtgacgatgatcctccc 347
|||||||||||||||||| ||||| |||||||||||||||||||||||||||||||| ||
Sbjct: 630 cttccgcgacgtcgtcaccggcgagttcgagaagttcacgagggtgacgatgatcctgcc 571
Query: 348 gctgacgggcgagcagtacgcggagaaagtgacggagaactgcgtggcgttctggaaggc 407
||||||||||| ||||||| |||| || ||||||||||||||||| |||| |||||||||
Sbjct: 570 gctgacgggcgcgcagtactcggacaaggtgacggagaactgcgtcgcgtactggaaggc 511
>gb|AF474923.1| Hordeum vulgare subsp. vulgare chalcone isomerase (CHI) gene,
complete cds
Length = 11475
Score = 151 bits (76), Expect = 2e-035
Identities = 144/166 (86%), Gaps = 3/166 (1%)
Strand = Plus / Plus
Query: 143 gcgcaggcgtgcgaggcctcgagatcggaggcaacttcatcaagttcacggccatcggcg 202
||||||||||||| || | |||||||| ||| |||||||||||||||||||||||||||
Sbjct: 7145 gcgcaggcgtgcgcgggatggagatcggcggccacttcatcaagttcacggccatcggcg 7204
Query: 203 tgtacctggagg---acgcggccgtgcccgcgctggccaagaagtggggcggcaagacgg 259
|||||||| ||| |||| ||||| ||||||| ||| ||||||| ||||||| | |
Sbjct: 7205 tgtacctgcaggccgacgccgccgtctccgcgctcgccgccaagtgggccggcaagcccg 7264
Query: 260 ccgacgagctcgcctccgacgccgccttcttccgcgacgtcgtcac 305
||| ||| ||||||||||||||||||||||||||||||||||||||
Sbjct: 7265 ccgccgatctcgcctccgacgccgccttcttccgcgacgtcgtcac 7310
Score = 121 bits (61), Expect = 2e-026
Identities = 91/101 (90%)
Strand = Plus / Plus
Query: 307 ggcgacttcgagaagttcacgagggtgacgatgatcctcccgctgacgggcgagcagtac 366
||||| ||||||||||||||||||||||| |||||||| ||||||||||||| |||||||
Sbjct: 7392 ggcgagttcgagaagttcacgagggtgacaatgatcctgccgctgacgggcgcgcagtac 7451
Query: 367 gcggagaaagtgacggagaactgcgtggcgttctggaaggc 407
|||| || |||||||||||||| || |||| |||||||||
Sbjct: 7452 tcggacaaggtgacggagaactgtgtcgcgtactggaaggc 7492
Score = 111 bits (56), Expect = 2e-023
Identities = 86/96 (89%)
Strand = Plus / Plus
Query: 54 catggccgtgccggaggtggtggtcgacggcgtcgtcttcccgccggtggcccgcccgcc 113
|||||||||| ||||| ||| ||||||||||||||||||||||||| | |||||||||||
Sbjct: 6936 catggccgtgtcggagctggaggtcgacggcgtcgtcttcccgccgctcgcccgcccgcc 6995
Query: 114 gggctccgccggctcgcacttcctcggcggcgcagg 149
|||||||||| | | |||||||||| |||||||||
Sbjct: 6996 gggctccgcccacgcccacttcctcgccggcgcagg 7031
>gb|BI957452.1|BI957452 HVSMEn0009K07f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0009K07f, mRNA sequence
Length = 668
Score = 99.6 bits (50), Expect = 7e-020
Identities = 65/70 (92%)
Strand = Plus / Plus
Query: 54 catggccgtgccggaggtggtggtcgacggcgtcgtcttcccgccggtggcccgcccgcc 113
|||||||||| ||||| ||| ||||||||||||||||||||||||| | |||||||||||
Sbjct: 83 catggccgtgtcggagctggaggtcgacggcgtcgtcttcccgccgctcgcccgcccgcc 142
Query: 114 gggctccgcc 123
||||||||||
Sbjct: 143 gggctccgcc 152
>gb|BI956899.1|BI956899 HVSMEn0005O19f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0005O19f, mRNA sequence
Length = 885
Score = 93.7 bits (47), Expect = 4e-018
Identities = 74/83 (89%)
Strand = Plus / Plus
Query: 325 acgagggtgacgatgatcctcccgctgacgggcgagcagtacgcggagaaagtgacggag 384
||||||||||| |||||||| ||||||||||||| ||||||| |||| || |||||||||
Sbjct: 20 acgagggtgacaatgatcctgccgctgacgggcgcgcagtactcggacaaggtgacggag 79
Query: 385 aactgcgtggcgttctggaaggc 407
||||| || |||| |||||||||
Sbjct: 80 aactgtgtcgcgtactggaaggc 102
>gb|CX662506.1|CX662506 EST 322 Expressed sequences from Barley Hordeum vulgare subsp.
vulgare cDNA clone P322 5', mRNA sequence
Length = 513
Score = 81.8 bits (41), Expect = 2e-014
Identities = 62/69 (89%)
Strand = Plus / Plus
Query: 339 gatcctcccgctgacgggcgagcagtacgcggagaaagtgacggagaactgcgtggcgtt 398
|||||| ||||||||||||| ||||||| |||| || ||||||||||||||||| ||||
Sbjct: 1 gatcctgccgctgacgggcgcgcagtactcggacaaggtgacggagaactgcgtcgcgta 60
Query: 399 ctggaaggc 407
|||||||||
Sbjct: 61 ctggaaggc 69
>gb|CX662646.1|CX662646 EST 477 Expressed sequences from Barley Hordeum vulgare subsp.
vulgare cDNA clone P477 5', mRNA sequence
Length = 513
Score = 81.8 bits (41), Expect = 2e-014
Identities = 62/69 (89%)
Strand = Plus / Plus
Query: 339 gatcctcccgctgacgggcgagcagtacgcggagaaagtgacggagaactgcgtggcgtt 398
|||||| ||||||||||||| ||||||| |||| || ||||||||||||||||| ||||
Sbjct: 1 gatcctgccgctgacgggcgcgcagtactcggacaaggtgacggagaactgcgtcgcgta 60
Query: 399 ctggaaggc 407
|||||||||
Sbjct: 61 ctggaaggc 69
>emb|AJ507395.1|HVU507395 Hordeum vulgare subsp. vulgare rpoT gene for DNA-directed RNA
polymerase, chloroplast precursor, exons 1-19
Length = 10573
Score = 42.1 bits (21), Expect = 0.014
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 94 ccgccggtggcccgcccgccg 114
|||||||||||||||||||||
Sbjct: 582 ccgccggtggcccgcccgccg 562
>emb|AJ507396.1|HVU507396 Hordeum vulgare subsp. vulgare mRNA for DNA-directed RNA
polymerase, chloroplast precursor (rpoT gene)
Length = 3655
Score = 42.1 bits (21), Expect = 0.014
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 94 ccgccggtggcccgcccgccg 114
|||||||||||||||||||||
Sbjct: 431 ccgccggtggcccgcccgccg 411
>gb|BQ762901.1|BQ762901 EBro02_SQ006_P10_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ006_P10 5', mRNA sequence
Length = 659
Score = 40.1 bits (20), Expect = 0.054
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 247 ggcggcaagacggccgacga 266
||||||||||||||||||||
Sbjct: 216 ggcggcaagacggccgacga 235
>gb|BM378047.2|BM378047 EBro08_SQ004_O21_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ004_O21 5', mRNA sequence
Length = 522
Score = 38.2 bits (19), Expect = 0.22
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 57 ggccgtgccggaggtggtg 75
|||||||||||||||||||
Sbjct: 103 ggccgtgccggaggtggtg 121
>gb|CA022183.1|CA022183 HZ42G07r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ42G07
5-PRIME, mRNA sequence
Length = 606
Score = 38.2 bits (19), Expect = 0.22
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 276 cgacgccgccttcttccgc 294
|||||||||||||||||||
Sbjct: 550 cgacgccgccttcttccgc 532
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 82,144
Number of Sequences: 312970
Number of extensions: 82144
Number of successful extensions: 24688
Number of sequences better than 0.5: 17
Number of HSP's better than 0.5 without gapping: 17
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 24659
Number of HSP's gapped (non-prelim): 23
length of query: 407
length of database: 175,134,539
effective HSP length: 18
effective length of query: 389
effective length of database: 169,501,079
effective search space: 65935919731
effective search space used: 65935919731
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)