BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3336981.2.1
(510 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE437893.1|BE437893 SFR008.F04F990628 ITEC SFR Barley Le... 86 1e-015
gb|AF427791.1| Hordeum vulgare Mla locus, complete sequence 42 0.017
gb|BF064540.1|BF064540 HV_CEb0015L05f Hordeum vulgare seedl... 38 0.27
gb|BG417352.2|BG417352 HVSMEk0017K17f Hordeum vulgare testa... 38 0.27
gb|BM373479.2|BM373479 EBma04_SQ004_J12_R maternal, 10 DPA,... 38 0.27
>gb|BE437893.1|BE437893 SFR008.F04F990628 ITEC SFR Barley Leaf Epidermis Library Hordeum
vulgare subsp. vulgare cDNA clone SF, mRNA sequence
Length = 614
Score = 85.7 bits (43), Expect = 1e-015
Identities = 61/67 (91%)
Strand = Plus / Plus
Query: 443 tcgtcggcgccggggtgcagggtgtcatgatgctcaacggcggcttcttccgcctcccca 502
||||||| ||||||||||| || || ||||||||||| |||||||||||||| |||||||
Sbjct: 2 tcgtcggggccggggtgcaaggggtgatgatgctcaatggcggcttcttccgtctcccca 61
Query: 503 acgagct 509
|||||||
Sbjct: 62 acgagct 68
>gb|AF427791.1| Hordeum vulgare Mla locus, complete sequence
Length = 261265
Score = 42.1 bits (21), Expect = 0.017
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 335 ccgaccacttcgtctacttcg 355
|||||||||||||||||||||
Sbjct: 48376 ccgaccacttcgtctacttcg 48396
>gb|BF064540.1|BF064540 HV_CEb0015L05f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0015L05f, mRNA sequence
Length = 599
Score = 38.2 bits (19), Expect = 0.27
Identities = 25/27 (92%)
Strand = Plus / Plus
Query: 435 ggggatcatcgtcggcgccggggtgca 461
|||||||| ||||||||||||| ||||
Sbjct: 149 ggggatcaccgtcggcgccgggatgca 175
>gb|BG417352.2|BG417352 HVSMEk0017K17f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0017K17f, mRNA sequence
Length = 684
Score = 38.2 bits (19), Expect = 0.27
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 404 tcatcgccgccgtcgtccc 422
|||||||||||||||||||
Sbjct: 515 tcatcgccgccgtcgtccc 497
>gb|BM373479.2|BM373479 EBma04_SQ004_J12_R maternal, 10 DPA, no treatment, cv Optic, EBma04
Hordeum vulgare subsp. vulgare cDNA clone
EBma04_SQ004_J12 5', mRNA sequence
Length = 417
Score = 38.2 bits (19), Expect = 0.27
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 404 tcatcgccgccgtcgtccc 422
|||||||||||||||||||
Sbjct: 37 tcatcgccgccgtcgtccc 19
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 65,479
Number of Sequences: 312970
Number of extensions: 65479
Number of successful extensions: 19732
Number of sequences better than 0.5: 5
Number of HSP's better than 0.5 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 19706
Number of HSP's gapped (non-prelim): 26
length of query: 510
length of database: 175,134,539
effective HSP length: 18
effective length of query: 492
effective length of database: 169,501,079
effective search space: 83394530868
effective search space used: 83394530868
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)