BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3276201.2.1
(1827 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA015721.1|CA015721 HV12G22u HV Hordeum vulgare subsp. v... 117 1e-024
gb|CB882318.1|CB882318 HL01I11w HL Hordeum vulgare subsp. v... 117 1e-024
gb|AF539980.1| Hordeum vulgare subsp. vulgare anther endoxy... 113 2e-023
gb|BQ471249.1|BQ471249 HV01N07T HV Hordeum vulgare subsp. v... 78 1e-012
gb|CA017926.1|CA017926 HV06N12r HV Hordeum vulgare subsp. v... 78 1e-012
gb|CA019576.1|CA019576 HV12G22r HV Hordeum vulgare subsp. v... 78 1e-012
gb|AJ461525.1|AJ461525 AJ461525 S00002 Hordeum vulgare subs... 70 3e-010
gb|BQ664105.1|BQ664105 HV01N07w HV Hordeum vulgare subsp. v... 60 3e-007
gb|CA016670.1|CA016670 HV06N12u HV Hordeum vulgare subsp. v... 60 3e-007
gb|AJ463273.1|AJ463273 AJ463273 S00002 Hordeum vulgare subs... 44 0.016
gb|AL506237.1|AL506237 AL506237 Hordeum vulgare Barke devel... 42 0.064
gb|AW982205.2|AW982205 HVSMEg0002E10f Hordeum vulgare pre-a... 42 0.064
gb|BI950704.1|BI950704 HVSMEl0022M04f Hordeum vulgare spike... 42 0.064
gb|BJ463425.1|BJ463425 BJ463425 K. Sato unpublished cDNA li... 42 0.064
gb|BQ460388.1|BQ460388 HA09M17r HA Hordeum vulgare subsp. v... 42 0.064
gb|BQ464047.1|BQ464047 HF01D07T HF Hordeum vulgare subsp. v... 42 0.064
gb|BQ471999.1|BQ471999 HV04C01r HV Hordeum vulgare subsp. v... 42 0.064
gb|BM376791.2|BM376791 EBem05_SQ003_E02_R embryo, 14 DPA, n... 42 0.064
gb|BM376958.2|BM376958 EBem05_SQ003_L10_R embryo, 14 DPA, n... 42 0.064
gb|BM373658.2|BM373658 EBma03_SQ002_E03_R maternal, 8 DPA, ... 42 0.064
gb|BM373914.2|BM373914 EBma03_SQ003_B17_R maternal, 8 DPA, ... 42 0.064
gb|BQ764615.1|BQ764615 EBca01_SQ004_K11_R carpel, pre-anthe... 42 0.064
gb|BU970112.1|BU970112 HB13K08r BC Hordeum vulgare subsp. v... 42 0.064
gb|BU988248.1|BU988248 HF17B16r HF Hordeum vulgare subsp. v... 42 0.064
gb|BU988629.1|BU988629 HF18D11r HF Hordeum vulgare subsp. v... 42 0.064
gb|BU997945.1|BU997945 HI09I01r HI Hordeum vulgare subsp. v... 42 0.064
>gb|CA015721.1|CA015721 HV12G22u HV Hordeum vulgare subsp. vulgare cDNA clone HV12G22
3-PRIME, mRNA sequence
Length = 542
Score = 117 bits (59), Expect = 1e-024
Identities = 83/91 (91%)
Strand = Plus / Minus
Query: 1243 gggctgctacgtcatgtgcctcacggacaacagcttcaagaacctccccgtgggcgacct 1302
|||||||||||||||||||||||| ||| ||| |||||| ||||||||||| ||||||
Sbjct: 470 gggctgctacgtcatgtgcctcaccgacgacaacttcaacaacctccccgtcggcgacac 411
Query: 1303 cgtcgacaagctcatcgccgagtggaagacg 1333
||||||||||||||||||||||||||||||
Sbjct: 410 ggtcgacaagctcatcgccgagtggaagacg 380
>gb|CB882318.1|CB882318 HL01I11w HL Hordeum vulgare subsp. vulgare cDNA clone HL01I11
3-PRIME, mRNA sequence
Length = 650
Score = 117 bits (59), Expect = 1e-024
Identities = 83/91 (91%)
Strand = Plus / Plus
Query: 1243 gggctgctacgtcatgtgcctcacggacaacagcttcaagaacctccccgtgggcgacct 1302
|||||||||||||||||||||||| ||| ||| |||||| ||||||||||| ||||||
Sbjct: 496 gggctgctacgtcatgtgcctcaccgacgacaacttcaacaacctccccgtcggcgacac 555
Query: 1303 cgtcgacaagctcatcgccgagtggaagacg 1333
||||||||||||||||||||||||||||||
Sbjct: 556 ggtcgacaagctcatcgccgagtggaagacg 586
Score = 77.8 bits (39), Expect = 1e-012
Identities = 57/63 (90%)
Strand = Plus / Plus
Query: 776 tacgccggcaaggtcatccactgggacgtggtgaacgagaacctgcacttcaacttcttc 835
|||||||||||||| ||| |||||||||||||||||||||||||||||| ||| ||||
Sbjct: 20 tacgccggcaaggtgatcgcgtgggacgtggtgaacgagaacctgcacttcgactacttc 79
Query: 836 gag 838
|||
Sbjct: 80 gag 82
Score = 56.0 bits (28), Expect = 4e-006
Identities = 31/32 (96%)
Strand = Plus / Plus
Query: 913 catgaacgaattcaacacgctggagcagcccg 944
||||||||| ||||||||||||||||||||||
Sbjct: 157 catgaacgacttcaacacgctggagcagcccg 188
Score = 44.1 bits (22), Expect = 0.016
Identities = 22/22 (100%)
Strand = Plus / Plus
Query: 1164 acctggagcaggtgctcaggga 1185
||||||||||||||||||||||
Sbjct: 417 acctggagcaggtgctcaggga 438
>gb|AF539980.1| Hordeum vulgare subsp. vulgare anther endoxylanase mRNA, partial cds
Length = 1169
Score = 113 bits (57), Expect = 2e-023
Identities = 90/101 (89%)
Strand = Plus / Plus
Query: 1253 gtcatgtgcctcacggacaacagcttcaagaacctccccgtgggcgacctcgtcgacaag 1312
|||||||||||||||||||||||||||| ||| |||||| ||| || |||||||||||
Sbjct: 706 gtcatgtgcctcacggacaacagcttcacgaatctccccaccggcaacgtcgtcgacaag 765
Query: 1313 ctcatcgccgagtggaagacgcaccgcgcgtccgccaccac 1353
||||||| ||||||||||||||||| || | ||||||||||
Sbjct: 766 ctcatcgacgagtggaagacgcaccccgtggccgccaccac 806
Score = 111 bits (56), Expect = 8e-023
Identities = 140/168 (83%)
Strand = Plus / Plus
Query: 967 caagtacgtggccaagatgaaccagatccgcggctacgccggcaacggtggcctcaagct 1026
|||||||| ||||||||| | ||||| ||| |||| ||||||||| ||| |||||||
Sbjct: 414 caagtacgccgccaagatggagcagatacgctcctaccccggcaacgacggcatcaagct 473
Query: 1027 cggcgtcggcctcgagagccacttcagcacacccaacatcccgtacatgaggagctcgct 1086
|| |||||||||||||||||||||| || ||||||||||| ||||||||| ||||
Sbjct: 474 cgccgtcggcctcgagagccacttcgagacgcccaacatcccatacatgagggcgacgct 533
Query: 1087 ggacacgctcgccaagctgaagctgcccatgtggctcaccgaggtgga 1134
||||| |||||| |||| ||| | ||||| |||||||||||||||||
Sbjct: 534 ggacatgctcgcgcagctcaaggtccccatctggctcaccgaggtgga 581
Score = 87.7 bits (44), Expect = 1e-015
Identities = 65/72 (90%)
Strand = Plus / Plus
Query: 554 gagaacgagatgaagtggtacagcaccgagtggacccagaaccatgaggactaccgcgtt 613
|||||||||||||||||||||||||||||||||| | ||||| ||||||||| ||||
Sbjct: 1 gagaacgagatgaagtggtacagcaccgagtggaagcggaaccgcgaggactacagcgtg 60
Query: 614 ccggacgccatg 625
||||||||||||
Sbjct: 61 ccggacgccatg 72
Score = 85.7 bits (43), Expect = 5e-015
Identities = 79/91 (86%)
Strand = Plus / Plus
Query: 729 agctcaaggcagccatgcagaagcggctcaagtccgtcgtgtcaccctacgccggcaagg 788
|||||||||| ||||||||||||||||||| ||| ||||| | | ||||||||||||||
Sbjct: 176 agctcaaggccgccatgcagaagcggctcagctccctcgtgacgcgctacgccggcaagg 235
Query: 789 tcatccactgggacgtggtgaacgagaacct 819
| ||| ||||||||| || |||||||||||
Sbjct: 236 tgatcgcctgggacgttgtcaacgagaacct 266
Score = 56.0 bits (28), Expect = 4e-006
Identities = 55/64 (85%)
Strand = Plus / Plus
Query: 1152 accaggtcaagtacctggagcaggtgctcagggaaggcttcgcccaccccaacgtcgacg 1211
||||||| ||||||||||| | ||||| |||||||||| || |||||||||||| || |
Sbjct: 605 accaggtggagtacctggaggacgtgctgagggaaggctacggccaccccaacgtggagg 664
Query: 1212 gcat 1215
||||
Sbjct: 665 gcat 668
>gb|BQ471249.1|BQ471249 HV01N07T HV Hordeum vulgare subsp. vulgare cDNA clone HV01N07
5-PRIME, mRNA sequence
Length = 449
Score = 77.8 bits (39), Expect = 1e-012
Identities = 57/63 (90%)
Strand = Plus / Plus
Query: 776 tacgccggcaaggtcatccactgggacgtggtgaacgagaacctgcacttcaacttcttc 835
|||||||||||||| ||| |||||||||||||||||||||||||||||| ||| ||||
Sbjct: 380 tacgccggcaaggtgatcgcgtgggacgtggtgaacgagaacctgcacttcgactacttc 439
Query: 836 gag 838
|||
Sbjct: 440 gag 442
Score = 77.8 bits (39), Expect = 1e-012
Identities = 42/43 (97%)
Strand = Plus / Plus
Query: 546 ccaccttcgagaacgagatgaagtggtacagcaccgagtggac 588
|||||||||||||||||||||||||||||||||| ||||||||
Sbjct: 150 ccaccttcgagaacgagatgaagtggtacagcacggagtggac 192
Score = 63.9 bits (32), Expect = 2e-008
Identities = 32/32 (100%)
Strand = Plus / Plus
Query: 655 ggtgcgcgggcacaacgtgttctgggacgacc 686
||||||||||||||||||||||||||||||||
Sbjct: 259 ggtgcgcgggcacaacgtgttctgggacgacc 290
>gb|CA017926.1|CA017926 HV06N12r HV Hordeum vulgare subsp. vulgare cDNA clone HV06N12
5-PRIME, mRNA sequence
Length = 500
Score = 77.8 bits (39), Expect = 1e-012
Identities = 57/63 (90%)
Strand = Plus / Plus
Query: 776 tacgccggcaaggtcatccactgggacgtggtgaacgagaacctgcacttcaacttcttc 835
|||||||||||||| ||| |||||||||||||||||||||||||||||| ||| ||||
Sbjct: 379 tacgccggcaaggtgatcgcgtgggacgtggtgaacgagaacctgcacttcgactacttc 438
Query: 836 gag 838
|||
Sbjct: 439 gag 441
Score = 77.8 bits (39), Expect = 1e-012
Identities = 42/43 (97%)
Strand = Plus / Plus
Query: 546 ccaccttcgagaacgagatgaagtggtacagcaccgagtggac 588
|||||||||||||||||||||||||||||||||| ||||||||
Sbjct: 149 ccaccttcgagaacgagatgaagtggtacagcacggagtggac 191
Score = 63.9 bits (32), Expect = 2e-008
Identities = 32/32 (100%)
Strand = Plus / Plus
Query: 655 ggtgcgcgggcacaacgtgttctgggacgacc 686
||||||||||||||||||||||||||||||||
Sbjct: 258 ggtgcgcgggcacaacgtgttctgggacgacc 289
>gb|CA019576.1|CA019576 HV12G22r HV Hordeum vulgare subsp. vulgare cDNA clone HV12G22
5-PRIME, mRNA sequence
Length = 410
Score = 77.8 bits (39), Expect = 1e-012
Identities = 42/43 (97%)
Strand = Plus / Plus
Query: 546 ccaccttcgagaacgagatgaagtggtacagcaccgagtggac 588
|||||||||||||||||||||||||||||||||| ||||||||
Sbjct: 262 ccaccttcgagaacgagatgaagtggtacagcacggagtggac 304
Score = 63.9 bits (32), Expect = 2e-008
Identities = 32/32 (100%)
Strand = Plus / Plus
Query: 655 ggtgcgcgggcacaacgtgttctgggacgacc 686
||||||||||||||||||||||||||||||||
Sbjct: 371 ggtgcgcgggcacaacgtgttctgggacgacc 402
>gb|AJ461525.1|AJ461525 AJ461525 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200024H04F1, mRNA sequence
Length = 300
Score = 69.9 bits (35), Expect = 3e-010
Identities = 47/51 (92%)
Strand = Plus / Plus
Query: 1283 aacctccccgtgggcgacctcgtcgacaagctcatcgccgagtggaagacg 1333
||||||||||| |||||| ||||||||||||||||||||||||||||||
Sbjct: 1 aacctccccgtcggcgacacggtcgacaagctcatcgccgagtggaagacg 51
>gb|BQ664105.1|BQ664105 HV01N07w HV Hordeum vulgare subsp. vulgare cDNA clone HV01N07
3-PRIME, mRNA sequence
Length = 421
Score = 60.0 bits (30), Expect = 3e-007
Identities = 30/30 (100%)
Strand = Plus / Minus
Query: 1304 gtcgacaagctcatcgccgagtggaagacg 1333
||||||||||||||||||||||||||||||
Sbjct: 407 gtcgacaagctcatcgccgagtggaagacg 378
>gb|CA016670.1|CA016670 HV06N12u HV Hordeum vulgare subsp. vulgare cDNA clone HV06N12
3-PRIME, mRNA sequence
Length = 421
Score = 60.0 bits (30), Expect = 3e-007
Identities = 30/30 (100%)
Strand = Plus / Minus
Query: 1304 gtcgacaagctcatcgccgagtggaagacg 1333
||||||||||||||||||||||||||||||
Sbjct: 411 gtcgacaagctcatcgccgagtggaagacg 382
>gb|AJ463273.1|AJ463273 AJ463273 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200025H09F1, mRNA sequence
Length = 180
Score = 44.1 bits (22), Expect = 0.016
Identities = 22/22 (100%)
Strand = Plus / Plus
Query: 1164 acctggagcaggtgctcaggga 1185
||||||||||||||||||||||
Sbjct: 95 acctggagcaggtgctcaggga 116
>gb|AL506237.1|AL506237 AL506237 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY02H15T 5',
mRNA sequence
Length = 697
Score = 42.1 bits (21), Expect = 0.064
Identities = 24/25 (96%)
Strand = Plus / Plus
Query: 1299 acctcgtcgacaagctcatcgccga 1323
|||||||||||||||||| ||||||
Sbjct: 106 acctcgtcgacaagctcaccgccga 130
>gb|AW982205.2|AW982205 HVSMEg0002E10f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0002E10f, mRNA sequence
Length = 828
Score = 42.1 bits (21), Expect = 0.064
Identities = 24/25 (96%)
Strand = Plus / Plus
Query: 1299 acctcgtcgacaagctcatcgccga 1323
|||||||||||||||||| ||||||
Sbjct: 230 acctcgtcgacaagctcaccgccga 254
>gb|BI950704.1|BI950704 HVSMEl0022M04f Hordeum vulgare spike EST library HVcDNA0012 (Fusarium
infected) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEl0022M04f, mRNA sequence
Length = 942
Score = 42.1 bits (21), Expect = 0.064
Identities = 24/25 (96%)
Strand = Plus / Plus
Query: 1299 acctcgtcgacaagctcatcgccga 1323
|||||||||||||||||| ||||||
Sbjct: 15 acctcgtcgacaagctcaccgccga 39
>gb|BJ463425.1|BJ463425 BJ463425 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags27k10 5', mRNA sequence
Length = 634
Score = 42.1 bits (21), Expect = 0.064
Identities = 24/25 (96%)
Strand = Plus / Plus
Query: 1299 acctcgtcgacaagctcatcgccga 1323
|||||||||||||||||| ||||||
Sbjct: 150 acctcgtcgacaagctcaccgccga 174
>gb|BQ460388.1|BQ460388 HA09M17r HA Hordeum vulgare subsp. vulgare cDNA clone HA09M17
5-PRIME, mRNA sequence
Length = 635
Score = 42.1 bits (21), Expect = 0.064
Identities = 24/25 (96%)
Strand = Plus / Plus
Query: 1299 acctcgtcgacaagctcatcgccga 1323
|||||||||||||||||| ||||||
Sbjct: 120 acctcgtcgacaagctcaccgccga 144
>gb|BQ464047.1|BQ464047 HF01D07T HF Hordeum vulgare subsp. vulgare cDNA clone HF01D07
5-PRIME, mRNA sequence
Length = 679
Score = 42.1 bits (21), Expect = 0.064
Identities = 24/25 (96%)
Strand = Plus / Plus
Query: 1299 acctcgtcgacaagctcatcgccga 1323
|||||||||||||||||| ||||||
Sbjct: 165 acctcgtcgacaagctcaccgccga 189
>gb|BQ471999.1|BQ471999 HV04C01r HV Hordeum vulgare subsp. vulgare cDNA clone HV04C01
5-PRIME, mRNA sequence
Length = 572
Score = 42.1 bits (21), Expect = 0.064
Identities = 24/25 (96%)
Strand = Plus / Plus
Query: 1299 acctcgtcgacaagctcatcgccga 1323
|||||||||||||||||| ||||||
Sbjct: 129 acctcgtcgacaagctcaccgccga 153
>gb|BM376791.2|BM376791 EBem05_SQ003_E02_R embryo, 14 DPA, no treatment, cv Optic, EBem05
Hordeum vulgare subsp. vulgare cDNA clone
EBem05_SQ003_E02 5', mRNA sequence
Length = 646
Score = 42.1 bits (21), Expect = 0.064
Identities = 24/25 (96%)
Strand = Plus / Plus
Query: 1299 acctcgtcgacaagctcatcgccga 1323
|||||||||||||||||| ||||||
Sbjct: 119 acctcgtcgacaagctcaccgccga 143
>gb|BM376958.2|BM376958 EBem05_SQ003_L10_R embryo, 14 DPA, no treatment, cv Optic, EBem05
Hordeum vulgare subsp. vulgare cDNA clone
EBem05_SQ003_L10 5', mRNA sequence
Length = 464
Score = 42.1 bits (21), Expect = 0.064
Identities = 24/25 (96%)
Strand = Plus / Plus
Query: 1299 acctcgtcgacaagctcatcgccga 1323
|||||||||||||||||| ||||||
Sbjct: 67 acctcgtcgacaagctcaccgccga 91
>gb|BM373658.2|BM373658 EBma03_SQ002_E03_R maternal, 8 DPA, no treatment, cv Optic, EBma03
Hordeum vulgare subsp. vulgare cDNA clone
EBma03_SQ002_E03 5', mRNA sequence
Length = 281
Score = 42.1 bits (21), Expect = 0.064
Identities = 24/25 (96%)
Strand = Plus / Plus
Query: 1299 acctcgtcgacaagctcatcgccga 1323
|||||||||||||||||| ||||||
Sbjct: 122 acctcgtcgacaagctcaccgccga 146
>gb|BM373914.2|BM373914 EBma03_SQ003_B17_R maternal, 8 DPA, no treatment, cv Optic, EBma03
Hordeum vulgare subsp. vulgare cDNA clone
EBma03_SQ003_B17 5', mRNA sequence
Length = 631
Score = 42.1 bits (21), Expect = 0.064
Identities = 24/25 (96%)
Strand = Plus / Plus
Query: 1299 acctcgtcgacaagctcatcgccga 1323
|||||||||||||||||| ||||||
Sbjct: 122 acctcgtcgacaagctcaccgccga 146
>gb|BQ764615.1|BQ764615 EBca01_SQ004_K11_R carpel, pre-anthesis, no treatment, cv Optic,
EBca01 Hordeum vulgare subsp. vulgare cDNA clone
EBca01_SQ004_K11 5', mRNA sequence
Length = 746
Score = 42.1 bits (21), Expect = 0.064
Identities = 24/25 (96%)
Strand = Plus / Plus
Query: 1299 acctcgtcgacaagctcatcgccga 1323
|||||||||||||||||| ||||||
Sbjct: 63 acctcgtcgacaagctcaccgccga 87
>gb|BU970112.1|BU970112 HB13K08r BC Hordeum vulgare subsp. vulgare cDNA clone HB13K08
5-PRIME, mRNA sequence
Length = 380
Score = 42.1 bits (21), Expect = 0.064
Identities = 24/25 (96%)
Strand = Plus / Plus
Query: 1299 acctcgtcgacaagctcatcgccga 1323
|||||||||||||||||| ||||||
Sbjct: 276 acctcgtcgacaagctcaccgccga 300
>gb|BU988248.1|BU988248 HF17B16r HF Hordeum vulgare subsp. vulgare cDNA clone HF17B16
5-PRIME, mRNA sequence
Length = 532
Score = 42.1 bits (21), Expect = 0.064
Identities = 24/25 (96%)
Strand = Plus / Plus
Query: 1299 acctcgtcgacaagctcatcgccga 1323
|||||||||||||||||| ||||||
Sbjct: 250 acctcgtcgacaagctcaccgccga 274
>gb|BU988629.1|BU988629 HF18D11r HF Hordeum vulgare subsp. vulgare cDNA clone HF18D11
5-PRIME, mRNA sequence
Length = 631
Score = 42.1 bits (21), Expect = 0.064
Identities = 24/25 (96%)
Strand = Plus / Plus
Query: 1299 acctcgtcgacaagctcatcgccga 1323
|||||||||||||||||| ||||||
Sbjct: 250 acctcgtcgacaagctcaccgccga 274
>gb|BU997945.1|BU997945 HI09I01r HI Hordeum vulgare subsp. vulgare cDNA clone HI09I01
5-PRIME, mRNA sequence
Length = 584
Score = 42.1 bits (21), Expect = 0.064
Identities = 24/25 (96%)
Strand = Plus / Plus
Query: 1299 acctcgtcgacaagctcatcgccga 1323
|||||||||||||||||| ||||||
Sbjct: 103 acctcgtcgacaagctcaccgccga 127
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 228,418
Number of Sequences: 312970
Number of extensions: 228418
Number of successful extensions: 70588
Number of sequences better than 0.5: 26
Number of HSP's better than 0.5 without gapping: 26
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 70531
Number of HSP's gapped (non-prelim): 56
length of query: 1827
length of database: 175,134,539
effective HSP length: 19
effective length of query: 1808
effective length of database: 169,188,109
effective search space: 305892101072
effective search space used: 305892101072
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)