BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3276099.2.1
(545 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV910514.1|AV910514 AV910514 K. Sato unpublished cDNA li... 170 3e-041
gb|BJ465220.1|BJ465220 BJ465220 K. Sato unpublished cDNA li... 170 3e-041
gb|BJ468559.1|BJ468559 BJ468559 K. Sato unpublished cDNA li... 170 3e-041
gb|AW982549.2|AW982549 HVSMEg0003J02f Hordeum vulgare pre-a... 163 7e-039
gb|BG344960.2|BG344960 HVSMEg0018A23f Hordeum vulgare pre-a... 147 4e-034
gb|BM368663.2|BM368663 EBem08_SQ004_E04_R embryo, 40 DPA, n... 103 6e-021
gb|BQ757871.1|BQ757871 EBem10_SQ005_H20_R embryo, 2 Day ger... 88 4e-016
>gb|AV910514.1|AV910514 AV910514 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak4m15 5', mRNA sequence
Length = 458
Score = 170 bits (86), Expect = 3e-041
Identities = 203/242 (83%)
Strand = Plus / Plus
Query: 21 gaaggcattgatcggtcgcattttcaggcgttggttcatttctacgggtgcaagcttcag 80
||||| ||||| || || ||||||||||| |||||||||||||||||||||||||||||
Sbjct: 78 gaagggattgaccgctcccattttcaggctctggttcatttctacgggtgcaagcttcag 137
Query: 81 gttcaagaaaggatgacaaggcagatagctgaagcggtttattcggtttcgcacaatggg 140
|| || |||||||||||||| |||||||||||||| || ||||| || || || |||
Sbjct: 138 gtccaggaaaggatgacaagacagatagctgaagcagtctattccgtctcacatcgtgga 197
Query: 141 gccatggtcgttgttgaggccaaccacatttgcatgatctcaagggggatagagaaaata 200
|| || || || || || ||||||||||| |||||||| |||||||| |||||||||||
Sbjct: 198 gctatagttgtcgtggaagccaaccacatctgcatgatatcaaggggaatagagaaaatc 257
Query: 201 aggagcaacacggcaacaattgcagttttgggccagtttttggccgacccttctgccaag 260
||||| | || ||||||||||||||||| || ||||| | | |||| |||||||||||
Sbjct: 258 aggagtagtacagcaacaattgcagttttaggtcagttctcgaccgattcttctgccaag 317
Query: 261 gc 262
||
Sbjct: 318 gc 319
>gb|BJ465220.1|BJ465220 BJ465220 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags38m09 5', mRNA sequence
Length = 480
Score = 170 bits (86), Expect = 3e-041
Identities = 203/242 (83%)
Strand = Plus / Plus
Query: 21 gaaggcattgatcggtcgcattttcaggcgttggttcatttctacgggtgcaagcttcag 80
||||| ||||| || || ||||||||||| |||||||||||||||||||||||||||||
Sbjct: 79 gaagggattgaccgctcccattttcaggctctggttcatttctacgggtgcaagcttcag 138
Query: 81 gttcaagaaaggatgacaaggcagatagctgaagcggtttattcggtttcgcacaatggg 140
|| || |||||||||||||| |||||||||||||| || ||||| || || || |||
Sbjct: 139 gtccaggaaaggatgacaagacagatagctgaagcagtctattccgtctcacatcgtgga 198
Query: 141 gccatggtcgttgttgaggccaaccacatttgcatgatctcaagggggatagagaaaata 200
|| || || || || || ||||||||||| |||||||| |||||||| |||||||||||
Sbjct: 199 gctatagttgtcgtggaagccaaccacatctgcatgatatcaaggggaatagagaaaatc 258
Query: 201 aggagcaacacggcaacaattgcagttttgggccagtttttggccgacccttctgccaag 260
||||| | || ||||||||||||||||| || ||||| | | |||| |||||||||||
Sbjct: 259 aggagtagtacagcaacaattgcagttttaggtcagttctcgaccgattcttctgccaag 318
Query: 261 gc 262
||
Sbjct: 319 gc 320
>gb|BJ468559.1|BJ468559 BJ468559 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags39f07 3', mRNA sequence
Length = 648
Score = 170 bits (86), Expect = 3e-041
Identities = 203/242 (83%)
Strand = Plus / Minus
Query: 21 gaaggcattgatcggtcgcattttcaggcgttggttcatttctacgggtgcaagcttcag 80
||||| ||||| || || ||||||||||| |||||||||||||||||||||||||||||
Sbjct: 643 gaagggattgaccgctcccattttcaggctctggttcatttctacgggtgcaagcttcag 584
Query: 81 gttcaagaaaggatgacaaggcagatagctgaagcggtttattcggtttcgcacaatggg 140
|| || |||||||||||||| |||||||||||||| || ||||| || || || |||
Sbjct: 583 gtccaggaaaggatgacaagacagatagctgaagcagtctattccgtctcacatcgtgga 524
Query: 141 gccatggtcgttgttgaggccaaccacatttgcatgatctcaagggggatagagaaaata 200
|| || || || || || ||||||||||| |||||||| |||||||| |||||||||||
Sbjct: 523 gctatagttgtcgtggaagccaaccacatctgcatgatatcaaggggaatagagaaaatc 464
Query: 201 aggagcaacacggcaacaattgcagttttgggccagtttttggccgacccttctgccaag 260
||||| | || ||||||||||||||||| || ||||| | | |||| |||||||||||
Sbjct: 463 aggagtagtacagcaacaattgcagttttaggtcagttctcgaccgattcttctgccaag 404
Query: 261 gc 262
||
Sbjct: 403 gc 402
Score = 44.1 bits (22), Expect = 0.005
Identities = 25/26 (96%)
Strand = Plus / Minus
Query: 466 gttttggttctctcgtgttgtaccat 491
|||||||||||| |||||||||||||
Sbjct: 132 gttttggttctcccgtgttgtaccat 107
>gb|AW982549.2|AW982549 HVSMEg0003J02f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0003J02f, mRNA sequence
Length = 562
Score = 163 bits (82), Expect = 7e-039
Identities = 202/242 (83%)
Strand = Plus / Plus
Query: 21 gaaggcattgatcggtcgcattttcaggcgttggttcatttctacgggtgcaagcttcag 80
||||| ||||| || || ||||||||||| | |||||||||||||||||||||||||||
Sbjct: 50 gaagggattgaccgctcccattttcaggctctcgttcatttctacgggtgcaagcttcag 109
Query: 81 gttcaagaaaggatgacaaggcagatagctgaagcggtttattcggtttcgcacaatggg 140
|| || |||||||||||||| |||||||||||||| || ||||| || || || |||
Sbjct: 110 gtccaggaaaggatgacaagacagatagctgaagcagtctattccgtctcacatcgtgga 169
Query: 141 gccatggtcgttgttgaggccaaccacatttgcatgatctcaagggggatagagaaaata 200
|| || || || || || ||||||||||| |||||||| |||||||| |||||||||||
Sbjct: 170 gctatagttgtcgtggaagccaaccacatctgcatgatatcaaggggaatagagaaaatc 229
Query: 201 aggagcaacacggcaacaattgcagttttgggccagtttttggccgacccttctgccaag 260
||||| | || ||||||||||||||||| || ||||| | | |||| |||||||||||
Sbjct: 230 aggagtagtacagcaacaattgcagttttaggtcagttctcgaccgattcttctgccaag 289
Query: 261 gc 262
||
Sbjct: 290 gc 291
>gb|BG344960.2|BG344960 HVSMEg0018A23f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0018A23f, mRNA sequence
Length = 625
Score = 147 bits (74), Expect = 4e-034
Identities = 201/242 (83%), Gaps = 1/242 (0%)
Strand = Plus / Plus
Query: 21 gaaggcattgatcggtcgcattttcaggcgttggttcatttctacgggtgcaagcttcag 80
||||| ||||| || || ||||||||||| | |||||||||||||||||||||||||||
Sbjct: 347 gaagggattgaccgctcccattttcaggctctcgttcatttctacgggtgcaagcttcag 406
Query: 81 gttcaagaaaggatgacaaggcagatagctgaagcggtttattcggtttcgcacaatggg 140
|| || |||||||||||||| |||||||||||||| || ||||| || || || |||
Sbjct: 407 gtccaggaaaggatgacaagacagatagctgaagcagtctattccgtctcacatcgtgga 466
Query: 141 gccatggtcgttgttgaggccaaccacatttgcatgatctcaagggggatagagaaaata 200
|| || || || || || ||||||||||| |||||||| |||||||| |||||| ||||
Sbjct: 467 gctatagttgtcgtggaagccaaccacatctgcatgatatcaaggggaatagag-aaatc 525
Query: 201 aggagcaacacggcaacaattgcagttttgggccagtttttggccgacccttctgccaag 260
||||| | || ||||||||||||||||| || ||||| | | |||| |||||||||||
Sbjct: 526 aggagtagtacagcaacaattgcagttttaggtcagttctcgaccgattcttctgccaag 585
Query: 261 gc 262
||
Sbjct: 586 gc 587
>gb|BM368663.2|BM368663 EBem08_SQ004_E04_R embryo, 40 DPA, no treatment, cv Optic, EBem08
Hordeum vulgare subsp. vulgare cDNA clone
EBem08_SQ004_E04 5', mRNA sequence
Length = 489
Score = 103 bits (52), Expect = 6e-021
Identities = 91/104 (87%)
Strand = Plus / Plus
Query: 21 gaaggcattgatcggtcgcattttcaggcgttggttcatttctacgggtgcaagcttcag 80
||||| ||||| || || ||||||||||| |||||||||||||||||||||||||||||
Sbjct: 385 gaagggattgaccgctcccattttcaggctctggttcatttctacgggtgcaagcttcag 444
Query: 81 gttcaagaaaggatgacaaggcagatagctgaagcggtttattc 124
|| || |||||||||||||| || ||| ||||||| || |||||
Sbjct: 445 gtccaggaaaggatgacaagacacatatctgaagcagtctattc 488
>gb|BQ757871.1|BQ757871 EBem10_SQ005_H20_R embryo, 2 Day germination, no treatment, cv
Optic, EBem10 Hordeum vulgare subsp. vulgare cDNA clone
EBem10_SQ005_H20 5', mRNA sequence
Length = 314
Score = 87.7 bits (44), Expect = 4e-016
Identities = 89/104 (85%)
Strand = Plus / Plus
Query: 159 gccaaccacatttgcatgatctcaagggggatagagaaaataaggagcaacacggcaaca 218
||||||||||| |||||||| |||||||| ||||||||||| ||||| | || ||||||
Sbjct: 32 gccaaccacatctgcatgatatcaaggggaatagagaaaatcaggagtagtacagcaaca 91
Query: 219 attgcagttttgggccagtttttggccgacccttctgccaaggc 262
||||||||||| || ||||| | | |||| |||||||||||||
Sbjct: 92 attgcagttttaggtcagttctcgaccgattcttctgccaaggc 135
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 47,043
Number of Sequences: 312970
Number of extensions: 47043
Number of successful extensions: 13215
Number of sequences better than 0.5: 8
Number of HSP's better than 0.5 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 13200
Number of HSP's gapped (non-prelim): 14
length of query: 545
length of database: 175,134,539
effective HSP length: 19
effective length of query: 526
effective length of database: 169,188,109
effective search space: 88992945334
effective search space used: 88992945334
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)