BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3230315.2.1
(457 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE422260.1|BE422260 HWM022cC.01r ITEC HWM Barley Leaf Li... 278 2e-073
gb|BJ468665.1|BJ468665 BJ468665 K. Sato unpublished cDNA li... 266 6e-070
gb|BM374246.2|BM374246 EBpi03_SQ003_B10_R pistil, 4 DPA, no... 258 1e-067
gb|AV919291.1|AV919291 AV919291 K. Sato unpublished cDNA li... 161 2e-038
gb|BQ661505.1|BQ661505 HM03L14u HM Hordeum vulgare subsp. v... 68 3e-010
gb|CB859122.1|CB859122 HI09G17w HI Hordeum vulgare subsp. v... 68 3e-010
gb|CB859989.1|CB859989 HI12E03w HI Hordeum vulgare subsp. v... 68 3e-010
gb|BG416061.2|BG416061 HVSMEk0009J20f Hordeum vulgare testa... 54 4e-006
gb|BG417076.2|BG417076 HVSMEk0016B22f Hordeum vulgare testa... 54 4e-006
gb|BU986911.1|BU986911 HF13E07r HF Hordeum vulgare subsp. v... 54 4e-006
gb|BU990192.1|BU990192 HF24F16r HF Hordeum vulgare subsp. v... 54 4e-006
gb|AV919302.1|AV919302 AV919302 K. Sato unpublished cDNA li... 52 2e-005
gb|BI950935.1|BI950935 HVSMEl0023L12f Hordeum vulgare spike... 44 0.004
gb|BI780849.2|BI780849 EBma05_SQ001_C24_R maternal, 12 DPA,... 44 0.004
gb|BQ765469.1|BQ765469 EBro03_SQ007_C09_R root, 3 week, wat... 44 0.004
gb|BJ463361.1|BJ463361 BJ463361 K. Sato unpublished cDNA li... 42 0.016
gb|AL503905.1|AL503905 AL503905 Hordeum vulgare Barke roots... 38 0.24
>gb|BE422260.1|BE422260 HWM022cC.01r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM022cC.01, mRNA sequence
Length = 1239
Score = 278 bits (140), Expect = 2e-073
Identities = 245/280 (87%)
Strand = Plus / Minus
Query: 165 gggatggacgtgccggcgagccagaggtcgccgtcgatgaagttgccaacggtgaaggcg 224
||||||||||||||||||||||||| |||||| ||||||||| ||| || ||||||| |
Sbjct: 397 gggatggacgtgccggcgagccagacgtcgccatcgatgaaggagcccaccgtgaaggtg 338
Query: 225 ctggcctcggcggcgctggtgatgacgcggtaccccttccacttgacccgcccggacgtg 284
|||||||| | |||||||||||||||||||||||||||||| | |||| |||||||||
Sbjct: 337 ctggcctccgatgcgctggtgatgacgcggtaccccttccacgtcaccctgccggacgtg 278
Query: 285 ccggcgccgggccccgtgttcatgtactcgccgtagtagagcgtgtcgagcgcgaagctg 344
||||||||||| ||||||||| |||||||||||||||||||||||||||||||||| ||
Sbjct: 277 ccggcgccgggtcccgtgttctggtactcgccgtagtagagcgtgtcgagcgcgaaggtg 218
Query: 345 ccgttccactcgagccagccggccgggtcgaccagcgagtccagctccgactgcaggtac 404
|||| |||| ||||||||||||| |||| ||| | ||| ||||||||||||| | |
Sbjct: 217 ccgtcccacgcgagccagccggcggggttgacgacgctgtcgagctccgactgcatgaag 158
Query: 405 accgtgcgggagtactgcttccatgggcggcccaggtagg 444
|| |||||||||||||||||||| || ||||| |||||||
Sbjct: 157 acggtgcgggagtactgcttccacggccggccaaggtagg 118
>gb|BJ468665.1|BJ468665 BJ468665 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags39m02 3', mRNA sequence
Length = 606
Score = 266 bits (134), Expect = 6e-070
Identities = 230/262 (87%)
Strand = Plus / Plus
Query: 165 gggatggacgtgccggcgagccagaggtcgccgtcgatgaagttgccaacggtgaaggcg 224
||||||||||||||||||||||||| |||||| ||||||||| ||| || ||||||| |
Sbjct: 345 gggatggacgtgccggcgagccagacgtcgccatcgatgaaggagcccaccgtgaaggtg 404
Query: 225 ctggcctcggcggcgctggtgatgacgcggtaccccttccacttgacccgcccggacgtg 284
|||||||| | |||||||||||||||||||||||||||||| | |||| |||||||||
Sbjct: 405 ctggcctccgatgcgctggtgatgacgcggtaccccttccacgtcaccctgccggacgtg 464
Query: 285 ccggcgccgggccccgtgttcatgtactcgccgtagtagagcgtgtcgagcgcgaagctg 344
||||||||||| ||||||||| |||||||||||||||||||||||||||||||||| ||
Sbjct: 465 ccggcgccgggtcccgtgttctggtactcgccgtagtagagcgtgtcgagcgcgaaggtg 524
Query: 345 ccgttccactcgagccagccggccgggtcgaccagcgagtccagctccgactgcaggtac 404
|||| |||| ||||||||||||| |||| ||| | ||| ||||||||||||| | |
Sbjct: 525 ccgtcccacgcgagccagccggcggggttgacgacgctgtcgagctccgactgcatgaag 584
Query: 405 accgtgcgggagtactgcttcc 426
|| |||||||||||||||||||
Sbjct: 585 acggtgcgggagtactgcttcc 606
>gb|BM374246.2|BM374246 EBpi03_SQ003_B10_R pistil, 4 DPA, no treatment, cv Optic, EBpi03
Hordeum vulgare subsp. vulgare cDNA clone
EBpi03_SQ003_B10 5', mRNA sequence
Length = 449
Score = 258 bits (130), Expect = 1e-067
Identities = 226/258 (87%)
Strand = Plus / Minus
Query: 165 gggatggacgtgccggcgagccagaggtcgccgtcgatgaagttgccaacggtgaaggcg 224
||||||||||||||||||||||||| |||||| ||||||||| ||| || ||||||| |
Sbjct: 258 gggatggacgtgccggcgagccagacgtcgccatcgatgaaggagcccaccgtgaaggtg 199
Query: 225 ctggcctcggcggcgctggtgatgacgcggtaccccttccacttgacccgcccggacgtg 284
|||||||| | |||||||||||||||||||||||||||||| | |||| |||||||||
Sbjct: 198 ctggcctccgatgcgctggtgatgacgcggtaccccttccacgtcaccctgccggacgtg 139
Query: 285 ccggcgccgggccccgtgttcatgtactcgccgtagtagagcgtgtcgagcgcgaagctg 344
||||||||||| ||||||||| |||||||||||||||||||||||||||||||||| ||
Sbjct: 138 ccggcgccgggtcccgtgttctggtactcgccgtagtagagcgtgtcgagcgcgaaggtg 79
Query: 345 ccgttccactcgagccagccggccgggtcgaccagcgagtccagctccgactgcaggtac 404
|||| |||| ||||||||||||| |||| ||| | ||| ||||||||||||| | |
Sbjct: 78 ccgtcccacgcgagccagccggcggggttgacgacgctgtcgagctccgactgcatgaag 19
Query: 405 accgtgcgggagtactgc 422
|| |||||||||||||||
Sbjct: 18 acggtgcgggagtactgc 1
>gb|AV919291.1|AV919291 AV919291 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags5g01 3', mRNA sequence
Length = 339
Score = 161 bits (81), Expect = 2e-038
Identities = 126/141 (89%)
Strand = Plus / Plus
Query: 165 gggatggacgtgccggcgagccagaggtcgccgtcgatgaagttgccaacggtgaaggcg 224
||||||||||||||||||||||||| |||||| ||||||||| ||| || ||||||| |
Sbjct: 194 gggatggacgtgccggcgagccagacgtcgccatcgatgaaggagcccaccgtgaaggtg 253
Query: 225 ctggcctcggcggcgctggtgatgacgcggtaccccttccacttgacccgcccggacgtg 284
|||||||| | |||||||||||||||||||||||||||||| | |||| |||||||||
Sbjct: 254 ctggcctccgatgcgctggtgatgacgcggtaccccttccacgtcaccctgccggacgtg 313
Query: 285 ccggcgccgggccccgtgttc 305
||||||||||| |||||||||
Sbjct: 314 ccggcgccgggtcccgtgttc 334
>gb|BQ661505.1|BQ661505 HM03L14u HM Hordeum vulgare subsp. vulgare cDNA clone HM03L14
3-PRIME, mRNA sequence
Length = 298
Score = 67.9 bits (34), Expect = 3e-010
Identities = 34/34 (100%)
Strand = Plus / Plus
Query: 308 gtactcgccgtagtagagcgtgtcgagcgcgaag 341
||||||||||||||||||||||||||||||||||
Sbjct: 243 gtactcgccgtagtagagcgtgtcgagcgcgaag 276
>gb|CB859122.1|CB859122 HI09G17w HI Hordeum vulgare subsp. vulgare cDNA clone HI09G17
3-PRIME, mRNA sequence
Length = 565
Score = 67.9 bits (34), Expect = 3e-010
Identities = 34/34 (100%)
Strand = Plus / Plus
Query: 308 gtactcgccgtagtagagcgtgtcgagcgcgaag 341
||||||||||||||||||||||||||||||||||
Sbjct: 242 gtactcgccgtagtagagcgtgtcgagcgcgaag 275
>gb|CB859989.1|CB859989 HI12E03w HI Hordeum vulgare subsp. vulgare cDNA clone HI12E03
3-PRIME, mRNA sequence
Length = 521
Score = 67.9 bits (34), Expect = 3e-010
Identities = 34/34 (100%)
Strand = Plus / Plus
Query: 308 gtactcgccgtagtagagcgtgtcgagcgcgaag 341
||||||||||||||||||||||||||||||||||
Sbjct: 243 gtactcgccgtagtagagcgtgtcgagcgcgaag 276
>gb|BG416061.2|BG416061 HVSMEk0009J20f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0009J20f, mRNA sequence
Length = 465
Score = 54.0 bits (27), Expect = 4e-006
Identities = 57/67 (85%)
Strand = Plus / Minus
Query: 263 ccacttgacccgcccggacgtgccggcgccgggccccgtgttcatgtactcgccgtagta 322
|||||| ||||||||| || |||||||| ||||| |||||| |||||| ||||||||
Sbjct: 151 ccacttcacccgcccgcctgtcccggcgccaggcccggtgttcccgtactccccgtagta 92
Query: 323 gagcgtg 329
|||||||
Sbjct: 91 gagcgtg 85
>gb|BG417076.2|BG417076 HVSMEk0016B22f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0016B22f, mRNA sequence
Length = 401
Score = 54.0 bits (27), Expect = 4e-006
Identities = 57/67 (85%)
Strand = Plus / Minus
Query: 263 ccacttgacccgcccggacgtgccggcgccgggccccgtgttcatgtactcgccgtagta 322
|||||| ||||||||| || |||||||| ||||| |||||| |||||| ||||||||
Sbjct: 110 ccacttcacccgcccgcctgtcccggcgccaggcccggtgttcccgtactccccgtagta 51
Query: 323 gagcgtg 329
|||||||
Sbjct: 50 gagcgtg 44
>gb|BU986911.1|BU986911 HF13E07r HF Hordeum vulgare subsp. vulgare cDNA clone HF13E07
5-PRIME, mRNA sequence
Length = 354
Score = 54.0 bits (27), Expect = 4e-006
Identities = 57/67 (85%)
Strand = Plus / Minus
Query: 263 ccacttgacccgcccggacgtgccggcgccgggccccgtgttcatgtactcgccgtagta 322
|||||| ||||||||| || |||||||| ||||| |||||| |||||| ||||||||
Sbjct: 76 ccacttcacccgcccgcctgtcccggcgccaggcccggtgttcccgtactccccgtagta 17
Query: 323 gagcgtg 329
|||||||
Sbjct: 16 gagcgtg 10
>gb|BU990192.1|BU990192 HF24F16r HF Hordeum vulgare subsp. vulgare cDNA clone HF24F16
5-PRIME, mRNA sequence
Length = 463
Score = 54.0 bits (27), Expect = 4e-006
Identities = 57/67 (85%)
Strand = Plus / Minus
Query: 263 ccacttgacccgcccggacgtgccggcgccgggccccgtgttcatgtactcgccgtagta 322
|||||| ||||||||| || |||||||| ||||| |||||| |||||| ||||||||
Sbjct: 184 ccacttcacccgcccgcctgtcccggcgccaggcccggtgttcccgtactccccgtagta 125
Query: 323 gagcgtg 329
|||||||
Sbjct: 124 gagcgtg 118
>gb|AV919302.1|AV919302 AV919302 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags5h01 3', mRNA sequence
Length = 194
Score = 52.0 bits (26), Expect = 2e-005
Identities = 42/48 (87%)
Strand = Plus / Plus
Query: 218 gaaggcgctggcctcggcggcgctggtgatgacgcggtaccccttcca 265
||||| ||||||||| | |||||||||| ||||||| ||||||||||
Sbjct: 147 gaaggtgctggcctccgatgcgctggtgangacgcggnaccccttcca 194
Score = 50.1 bits (25), Expect = 6e-005
Identities = 27/28 (96%)
Strand = Plus / Plus
Query: 161 gaaggggatggacgtgccggcgagccag 188
||| ||||||||||||||||||||||||
Sbjct: 89 gaangggatggacgtgccggcgagccag 116
>gb|BI950935.1|BI950935 HVSMEl0023L12f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0023L12f, mRNA sequence
Length = 536
Score = 44.1 bits (22), Expect = 0.004
Identities = 40/46 (86%)
Strand = Plus / Minus
Query: 308 gtactcgccgtagtagagcgtgtcgagcgcgaagctgccgttccac 353
||||||| ||||||||||||| | |||| ||||| ||||||||||
Sbjct: 451 gtactcggcgtagtagagcgtcttgagcccgaagtcgccgttccac 406
>gb|BI780849.2|BI780849 EBma05_SQ001_C24_R maternal, 12 DPA, no treatment, cv Optic, EBma05
Hordeum vulgare subsp. vulgare cDNA clone
EBma05_SQ001_C24 5', mRNA sequence
Length = 602
Score = 44.1 bits (22), Expect = 0.004
Identities = 40/46 (86%)
Strand = Plus / Minus
Query: 308 gtactcgccgtagtagagcgtgtcgagcgcgaagctgccgttccac 353
||||||| ||||||||||||| | |||| ||||| ||||||||||
Sbjct: 602 gtactcggcgtagtagagcgtcttgagcccgaagtcgccgttccac 557
>gb|BQ765469.1|BQ765469 EBro03_SQ007_C09_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ007_C09 5', mRNA sequence
Length = 417
Score = 44.1 bits (22), Expect = 0.004
Identities = 31/34 (91%)
Strand = Plus / Minus
Query: 308 gtactcgccgtagtagagcgtgtcgagcgcgaag 341
||||||| |||||||||||||| ||||||||||
Sbjct: 121 gtactcggcgtagtagagcgtgctgagcgcgaag 88
>gb|BJ463361.1|BJ463361 BJ463361 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags27c22 5', mRNA sequence
Length = 648
Score = 42.1 bits (21), Expect = 0.016
Identities = 24/25 (96%)
Strand = Plus / Plus
Query: 63 caaacgacgaacttccgccgccgcc 87
||||||||||||| |||||||||||
Sbjct: 43 caaacgacgaactcccgccgccgcc 67
>gb|AL503905.1|AL503905 AL503905 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW03M21T 5', mRNA sequence
Length = 502
Score = 38.2 bits (19), Expect = 0.24
Identities = 19/19 (100%)
Strand = Plus / Minus
Query: 243 gtgatgacgcggtacccct 261
|||||||||||||||||||
Sbjct: 451 gtgatgacgcggtacccct 433
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 104,091
Number of Sequences: 312970
Number of extensions: 104091
Number of successful extensions: 25914
Number of sequences better than 0.5: 17
Number of HSP's better than 0.5 without gapping: 17
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 25878
Number of HSP's gapped (non-prelim): 25
length of query: 457
length of database: 175,134,539
effective HSP length: 18
effective length of query: 439
effective length of database: 169,501,079
effective search space: 74410973681
effective search space used: 74410973681
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)