BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3204398.2.1
(666 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE559029.2|BE559029 HV_CEb0022G04f Hordeum vulgare seedl... 236 8e-061
gb|AL505620.1|AL505620 AL505620 Hordeum vulgare Barke roots... 230 5e-059
gb|BQ663389.1|BQ663389 HU02G23u HU Hordeum vulgare subsp. v... 228 2e-058
gb|AL502881.1|AL502881 AL502881 Hordeum vulgare Barke roots... 190 4e-047
gb|BM442847.2|BM442847 EBro02_SQ001_I13_R root, 3 week, hyd... 105 2e-021
gb|BM442973.2|BM442973 EBro02_SQ002_H11_R root, 3 week, hyd... 90 1e-016
gb|BQ762130.1|BQ762130 EBpi01_SQ005_L22_R pistil, 1 DPA, no... 64 6e-009
gb|BU984325.1|BU984325 HF03J23r HF Hordeum vulgare subsp. v... 38 0.36
gb|BU987344.1|BU987344 HF14I02r HF Hordeum vulgare subsp. v... 38 0.36
>gb|BE559029.2|BE559029 HV_CEb0022G04f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0022G04f, mRNA sequence
Length = 669
Score = 236 bits (119), Expect = 8e-061
Identities = 230/267 (86%)
Strand = Plus / Plus
Query: 1 acatctggaaactttgaattcgcacttttagctatcctcagatgcgcggaaaacccagca 60
|||||||||||||||||| | || || || ||||||||||||| || || || |||||
Sbjct: 153 acatctggaaactttgaagttgctctgataactatcctcagatgtgctgagaatccagct 212
Query: 61 aagtattttgcaaagctcttacgaaaggccatgaaaggtctaggcactgatgacaagaca 120
||||||||||| ||| | ||| | ||| |||||||||||||||| |||||||||||||||
Sbjct: 213 aagtattttgctaaggtgttaaggaagtccatgaaaggtctaggtactgatgacaagaca 272
Query: 121 cttataagggttgtggtgacgaggactgaaattgatatgcaatatatcaaggcagagtac 180
||| ||||||||||||| ||||||||||| ||||||||||| |||||||||||||| |||
Sbjct: 273 cttgtaagggttgtggtaacgaggactgagattgatatgcagtatatcaaggcagaatac 332
Query: 181 ttcaagaagtataagaagccattagctgaggctatccactccgaaacatcaggaaactat 240
| |||||| || || ||||| |||||||| |||||||| || |||||||||||| |||
Sbjct: 333 tacaagaaatacaaaaagccgttagctgatgctatccattcagaaacatcaggaggttat 392
Query: 241 cggacattccttctttcacttgttggt 267
||||| ||||| ||||| |||||||||
Sbjct: 393 cggacgttcctgctttctcttgttggt 419
>gb|AL505620.1|AL505620 AL505620 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW09A09V 5', mRNA sequence
Length = 468
Score = 230 bits (116), Expect = 5e-059
Identities = 227/264 (85%)
Strand = Plus / Plus
Query: 1 acatctggaaactttgaattcgcacttttagctatcctcagatgcgcggaaaacccagca 60
|||||||||||||||||| | || || || ||||||||||||| || || || |||||
Sbjct: 205 acatctggaaactttgaagttgctctgataactatcctcagatgtgctgagaatccagct 264
Query: 61 aagtattttgcaaagctcttacgaaaggccatgaaaggtctaggcactgatgacaagaca 120
||||||||||| ||| | ||| | ||| |||||||||||||||| |||||||||||||||
Sbjct: 265 aagtattttgctaaggtgttaaggaagtccatgaaaggtctaggtactgatgacaagaca 324
Query: 121 cttataagggttgtggtgacgaggactgaaattgatatgcaatatatcaaggcagagtac 180
||| ||||||||||||| ||||||||||| ||||||||||| |||||||||||||| |||
Sbjct: 325 cttgtaagggttgtggtaacgaggactgagattgatatgcagtatatcaaggcagaatac 384
Query: 181 ttcaagaagtataagaagccattagctgaggctatccactccgaaacatcaggaaactat 240
| |||||| || || ||||| |||||||| |||||||| || |||||||||||| |||
Sbjct: 385 tacaagaaatacaaaaagccgttagctgatgctatccattcagaaacatcaggaggttat 444
Query: 241 cggacattccttctttcacttgtt 264
||||| ||||| ||||| ||||||
Sbjct: 445 cggacgttcctgctttctcttgtt 468
>gb|BQ663389.1|BQ663389 HU02G23u HU Hordeum vulgare subsp. vulgare cDNA clone HU02G23
3-PRIME, mRNA sequence
Length = 495
Score = 228 bits (115), Expect = 2e-058
Identities = 229/267 (85%)
Strand = Plus / Minus
Query: 1 acatctggaaactttgaattcgcacttttagctatcctcagatgcgcggaaaacccagca 60
|||||||||||||||||| | || || || ||||||||||||| || || || |||||
Sbjct: 386 acatctggaaactttgaagttgctctgataactatcctcagatgtgctgagaatccagct 327
Query: 61 aagtattttgcaaagctcttacgaaaggccatgaaaggtctaggcactgatgacaagaca 120
||||||||||| ||| | ||| | ||| |||||||||||||||| |||||||||||||||
Sbjct: 326 aagtattttgctaaggtgttaaggaagtccatgaaaggtctaggtactgatgacaagaca 267
Query: 121 cttataagggttgtggtgacgaggactgaaattgatatgcaatatatcaaggcagagtac 180
||| ||||||||||||| ||||||||||| ||||||||||| |||||||||||||| |||
Sbjct: 266 cttgtaagggttgtggtaacgaggactgagattgatatgcagtatatcaaggcagaatac 207
Query: 181 ttcaagaagtataagaagccattagctgaggctatccactccgaaacatcaggaaactat 240
| |||||| || || ||||| |||||||| |||||||| || ||||||||||| |||
Sbjct: 206 tacaagaaatacaaaaagccgttagctgatgctatccattcaaaaacatcaggaggttat 147
Query: 241 cggacattccttctttcacttgttggt 267
||||| ||||| ||||| |||||||||
Sbjct: 146 cggacgttcctgctttctcttgttggt 120
>gb|AL502881.1|AL502881 AL502881 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW09A09u 3', mRNA sequence
Length = 658
Score = 190 bits (96), Expect = 4e-047
Identities = 181/208 (87%), Gaps = 1/208 (0%)
Strand = Plus / Minus
Query: 61 aagtattttgcaaagctcttacgaaaggccatgaaaggtc-taggcactgatgacaagac 119
||||||||||| ||| | ||| | ||| |||||||||||| |||| ||||||||||||||
Sbjct: 534 aagtattttgctaaggtgttaaggaagtccatgaaaggtcntaggtactgatgacaagac 475
Query: 120 acttataagggttgtggtgacgaggactgaaattgatatgcaatatatcaaggcagagta 179
|||| ||||||||||||| ||||||||||| ||||||||||| |||||||||||||| ||
Sbjct: 474 acttgtaagggttgtggtaacgaggactgagattgatatgcagtatatcaaggcagaata 415
Query: 180 cttcaagaagtataagaagccattagctgaggctatccactccgaaacatcaggaaacta 239
|| |||||| || || ||||| |||||||| |||||||| || |||||||||||| ||
Sbjct: 414 ctacaagaaatacaaaaagccgttagctgatgctatccattcagaaacatcaggaggtta 355
Query: 240 tcggacattccttctttcacttgttggt 267
|||||| ||||| ||||| |||||||||
Sbjct: 354 tcggacgttcctgctttctcttgttggt 327
>gb|BM442847.2|BM442847 EBro02_SQ001_I13_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ001_I13 5', mRNA sequence
Length = 445
Score = 105 bits (53), Expect = 2e-021
Identities = 101/117 (86%)
Strand = Plus / Plus
Query: 151 attgatatgcaatatatcaaggcagagtacttcaagaagtataagaagccattagctgag 210
||||||||||| |||||||||||||| |||| |||||| || || ||||| ||||||||
Sbjct: 2 attgatatgcagtatatcaaggcagaatactacaagaaatacaaaaagccgttagctgat 61
Query: 211 gctatccactccgaaacatcaggaaactatcggacattccttctttcacttgttggt 267
|||||||| || |||||||||||| |||||||| ||||| ||||| |||||||||
Sbjct: 62 gctatccattcagaaacatcaggaggttatcggacgttcctgctttctcttgttggt 118
>gb|BM442973.2|BM442973 EBro02_SQ002_H11_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ002_H11 5', mRNA sequence
Length = 424
Score = 89.7 bits (45), Expect = 1e-016
Identities = 90/105 (85%)
Strand = Plus / Plus
Query: 163 tatatcaaggcagagtacttcaagaagtataagaagccattagctgaggctatccactcc 222
|||||||||||||| |||| |||||| || || ||||| |||||||| |||||||| ||
Sbjct: 2 tatatcaaggcagaatactacaagaaatacaaaaagccgttagctgatgctatccattca 61
Query: 223 gaaacatcaggaaactatcggacattccttctttcacttgttggt 267
|||||||||||| |||||||| ||||| ||||| |||||||||
Sbjct: 62 gaaacatcaggaggttatcggacgttcctgctttctcttgttggt 106
>gb|BQ762130.1|BQ762130 EBpi01_SQ005_L22_R pistil, 1 DPA, no treatment, cv Optic, EBpi01
Hordeum vulgare subsp. vulgare cDNA clone
EBpi01_SQ005_L22 5', mRNA sequence
Length = 420
Score = 63.9 bits (32), Expect = 6e-009
Identities = 62/72 (86%)
Strand = Plus / Plus
Query: 196 aagccattagctgaggctatccactccgaaacatcaggaaactatcggacattccttctt 255
||||| |||||||| |||||||| || |||||||||||| |||||||| ||||| |||
Sbjct: 5 aagccgttagctgatgctatccattcagaaacatcaggaggttatcggacgttcctgctt 64
Query: 256 tcacttgttggt 267
|| |||||||||
Sbjct: 65 tctcttgttggt 76
>gb|BU984325.1|BU984325 HF03J23r HF Hordeum vulgare subsp. vulgare cDNA clone HF03J23
5-PRIME, mRNA sequence
Length = 569
Score = 38.2 bits (19), Expect = 0.36
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 490 cagctgttgttggtcacag 508
|||||||||||||||||||
Sbjct: 397 cagctgttgttggtcacag 415
>gb|BU987344.1|BU987344 HF14I02r HF Hordeum vulgare subsp. vulgare cDNA clone HF14I02
5-PRIME, mRNA sequence
Length = 644
Score = 38.2 bits (19), Expect = 0.36
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 490 cagctgttgttggtcacag 508
|||||||||||||||||||
Sbjct: 473 cagctgttgttggtcacag 491
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 46,808
Number of Sequences: 312970
Number of extensions: 46808
Number of successful extensions: 11297
Number of sequences better than 0.5: 9
Number of HSP's better than 0.5 without gapping: 9
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 11286
Number of HSP's gapped (non-prelim): 10
length of query: 666
length of database: 175,134,539
effective HSP length: 19
effective length of query: 647
effective length of database: 169,188,109
effective search space: 109464706523
effective search space used: 109464706523
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)