BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3198833.2.1
(593 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI950935.1|BI950935 HVSMEl0023L12f Hordeum vulgare spike... 706 0.0
gb|BI780849.2|BI780849 EBma05_SQ001_C24_R maternal, 12 DPA,... 670 0.0
gb|BJ465418.1|BJ465418 BJ465418 K. Sato unpublished cDNA li... 82 2e-014
gb|BQ754059.1|BQ754059 EBca01_SQ002_L07_R carpel, pre-anthe... 80 9e-014
gb|CK122193.1|CK122193 BES1824101p15 BES1824 Hordeum vulgar... 54 5e-006
gb|CK122985.1|CK122985 BES1824101k13 BES1824 Hordeum vulgar... 54 5e-006
gb|BQ765469.1|BQ765469 EBro03_SQ007_C09_R root, 3 week, wat... 50 8e-005
gb|AV914255.1|AV914255 AV914255 K. Sato unpublished cDNA li... 48 3e-004
gb|AV914270.1|AV914270 AV914270 K. Sato unpublished cDNA li... 48 3e-004
gb|BQ661505.1|BQ661505 HM03L14u HM Hordeum vulgare subsp. v... 46 0.001
gb|CB859122.1|CB859122 HI09G17w HI Hordeum vulgare subsp. v... 46 0.001
gb|CB859989.1|CB859989 HI12E03w HI Hordeum vulgare subsp. v... 46 0.001
gb|CV062610.1|CV062610 BNEL80h9 Barley EST endosperm librar... 42 0.020
gb|BE422260.1|BE422260 HWM022cC.01r ITEC HWM Barley Leaf Li... 38 0.32
gb|BI960087.1|BI960087 HVSMEn0023C13f Hordeum vulgare rachi... 38 0.32
gb|BJ468665.1|BJ468665 BJ468665 K. Sato unpublished cDNA li... 38 0.32
gb|BM374246.2|BM374246 EBpi03_SQ003_B10_R pistil, 4 DPA, no... 38 0.32
>gb|BI950935.1|BI950935 HVSMEl0023L12f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0023L12f, mRNA sequence
Length = 536
Score = 706 bits (356), Expect = 0.0
Identities = 482/524 (91%)
Strand = Plus / Plus
Query: 58 cagggagacctcgcggcgttctacaactgccggttcgacgcgttccaggacacgctgtac 117
||||| ||||| |||| |||| |||||||||||| ||||||||||||||||||||||||
Sbjct: 11 cagggcgacctgtcggccttcttcaactgccggtttgacgcgttccaggacacgctgtac 70
Query: 118 gtgcacgcccggcgccagttcttccgcaactgcgtcatctccggcaccattgacttcatc 177
|||||||||||||| ||||||||||||||||||||||||||||||||||| |||||||||
Sbjct: 71 gtgcacgcccggcggcagttcttccgcaactgcgtcatctccggcaccatcgacttcatc 130
Query: 178 ttcggcaactcggcggcggtgttccagaactgcctcatcgtcacgcggcggcccatggac 237
||||||||||||||||| ||||||||||||||||||||| ||||||||||||||||||||
Sbjct: 131 ttcggcaactcggcggccgtgttccagaactgcctcatcatcacgcggcggcccatggac 190
Query: 238 aaccagcagaactcggtgaccgcgcacggccgcacggaccccaacatgaagtcggggctc 297
||||||||||||||||||||||||||||| ||||||||||||||||||||||| |||||
Sbjct: 191 aaccagcagaactcggtgaccgcgcacgggcgcacggaccccaacatgaagtccgggctg 250
Query: 298 gtcatccagaactgccgcctggtgcccgaccagaagctcttccccgaccgcttcaagatc 357
|||||||||||||||||||||||||| ||||||||||||||| ||||||||||| |||
Sbjct: 251 gtcatccagaactgccgcctggtgccggaccagaagctcttcgtggaccgcttcaaaatc 310
Query: 358 ccctcgtacctgggccgaccctggaaggagttctcgcgcctcgtcatcatggagagcacc 417
|| || ||||||||| | || ||||||||||||||||| || |||||||||||||||
Sbjct: 311 ccgtcctacctgggcaggccgtggaaggagttctcgcggctggtcatcatggagagccag 370
Query: 418 atcgccgacttcatcaagcccgagggctacatgccctggaacggcgacttcggcatcaag 477
|||||||||||||||||||||||||| |||||||| ||||||||||||||||| |||||
Sbjct: 371 atcgccgacttcatcaagcccgaggggtacatgccgtggaacggcgacttcgggctcaag 430
Query: 478 acgctctactacgccgagtacaacaaccgcggccccggcgccggcaccagcaagagggtc 537
||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
Sbjct: 431 acgctctactacgccgagtacggcaaccgcggccccggcgccggcaccagcaagagggtc 490
Query: 538 acctggcctgggttccacgtcatcggccggaaggacgccgagca 581
|||||||| || |||| ||||||| | || |||| ||||||||
Sbjct: 491 acctggccgggcttccgagtcatcgccaggcaggaggccgagca 534
>gb|BI780849.2|BI780849 EBma05_SQ001_C24_R maternal, 12 DPA, no treatment, cv Optic, EBma05
Hordeum vulgare subsp. vulgare cDNA clone
EBma05_SQ001_C24 5', mRNA sequence
Length = 602
Score = 670 bits (338), Expect = 0.0
Identities = 458/498 (91%)
Strand = Plus / Plus
Query: 1 atgggcttccacaacacggccggcgcggagaggcaccaggcggtggcgctccgcgttcag 60
||||| |||||||||||||| |||||||||| ||||||||||||||||| | || |||
Sbjct: 105 atggggttccacaacacggcgggcgcggagaaacaccaggcggtggcgctgagggtgcag 164
Query: 61 ggagacctcgcggcgttctacaactgccggttcgacgcgttccaggacacgctgtacgtg 120
|| ||||| |||| |||| |||||||||||| |||||||||||||||||||||||||||
Sbjct: 165 ggcgacctgtcggccttcttcaactgccggtttgacgcgttccaggacacgctgtacgtg 224
Query: 121 cacgcccggcgccagttcttccgcaactgcgtcatctccggcaccattgacttcatcttc 180
||||||||||| ||||||||||||||||||||||||||||||||||| ||||||||||||
Sbjct: 225 cacgcccggcggcagttcttccgcaactgcgtcatctccggcaccatcgacttcatcttc 284
Query: 181 ggcaactcggcggcggtgttccagaactgcctcatcgtcacgcggcggcccatggacaac 240
|||||||||||||| ||||||||||||||||||||| |||||||||||||||||||||||
Sbjct: 285 ggcaactcggcggccgtgttccagaactgcctcatcatcacgcggcggcccatggacaac 344
Query: 241 cagcagaactcggtgaccgcgcacggccgcacggaccccaacatgaagtcggggctcgtc 300
|||||||||||||||||||||||||| ||||||||||||||||||||||| ||||| |||
Sbjct: 345 cagcagaactcggtgaccgcgcacgggcgcacggaccccaacatgaagtccgggctggtc 404
Query: 301 atccagaactgccgcctggtgcccgaccagaagctcttccccgaccgcttcaagatcccc 360
||||||||||||||||||||||| ||||||||||||||| ||||||||||| |||||
Sbjct: 405 atccagaactgccgcctggtgccggaccagaagctcttcgtggaccgcttcaaaatcccg 464
Query: 361 tcgtacctgggccgaccctggaaggagttctcgcgcctcgtcatcatggagagcaccatc 420
|| ||||||||| | || ||||||||||||||||| || ||||||||||||||| |||
Sbjct: 465 tcctacctgggcaggccgtggaaggagttctcgcggctggtcatcatggagagccagatc 524
Query: 421 gccgacttcatcaagcccgagggctacatgccctggaacggcgacttcggcatcaagacg 480
||||||||||||||||||||||| |||||||| ||||||||||||||||| ||||||||
Sbjct: 525 gccgacttcatcaagcccgaggggtacatgccgtggaacggcgacttcgggctcaagacg 584
Query: 481 ctctactacgccgagtac 498
||||||||||||||||||
Sbjct: 585 ctctactacgccgagtac 602
>gb|BJ465418.1|BJ465418 BJ465418 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags39m02 5', mRNA sequence
Length = 528
Score = 81.8 bits (41), Expect = 2e-014
Identities = 92/109 (84%)
Strand = Plus / Plus
Query: 102 ccaggacacgctgtacgtgcacgcccggcgccagttcttccgcaactgcgtcatctccgg 161
||||||||| || ||||||||| ||| || |||||||||||| | |||| ||||| |||
Sbjct: 306 ccaggacaccctctacgtgcactccctccgtcagttcttccgcgagtgcgacatctacgg 365
Query: 162 caccattgacttcatcttcggcaactcggcggcggtgttccagaactgc 210
|||||| |||||| ||||||||||||| || || ||| |||||| ||||
Sbjct: 366 caccatcgacttcgtcttcggcaactccgccgccgtgctccagagctgc 414
Score = 48.1 bits (24), Expect = 3e-004
Identities = 24/24 (100%)
Strand = Plus / Plus
Query: 33 gcaccaggcggtggcgctccgcgt 56
||||||||||||||||||||||||
Sbjct: 237 gcaccaggcggtggcgctccgcgt 260
>gb|BQ754059.1|BQ754059 EBca01_SQ002_L07_R carpel, pre-anthesis, no treatment, cv Optic,
EBca01 Hordeum vulgare subsp. vulgare cDNA clone
EBca01_SQ002_L07 5', mRNA sequence
Length = 372
Score = 79.8 bits (40), Expect = 9e-014
Identities = 94/112 (83%)
Strand = Plus / Plus
Query: 102 ccaggacacgctgtacgtgcacgcccggcgccagttcttccgcaactgcgtcatctccgg 161
||||||||||||||||| |||| | | |||||||||| |||| ||||| | || ||||
Sbjct: 112 ccaggacacgctgtacgcgcactcgctccgccagttctaccgcgactgccgcgtcgccgg 171
Query: 162 caccattgacttcatcttcggcaactcggcggcggtgttccagaactgcctc 213
|||| | |||||| ||||||||||| | || || ||||||||||||||||||
Sbjct: 172 caccgtcgacttcgtcttcggcaacgccgccgccgtgttccagaactgcctc 223
>gb|CK122193.1|CK122193 BES1824101p15 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010P151 5-PRIME, mRNA sequence
Length = 641
Score = 54.0 bits (27), Expect = 5e-006
Identities = 33/35 (94%)
Strand = Plus / Plus
Query: 169 gacttcatcttcggcaactcggcggcggtgttcca 203
|||||||||||||||||||||||||| ||| ||||
Sbjct: 373 gacttcatcttcggcaactcggcggccgtgctcca 407
>gb|CK122985.1|CK122985 BES1824101k13 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010K131 5-PRIME, mRNA sequence
Length = 611
Score = 54.0 bits (27), Expect = 5e-006
Identities = 33/35 (94%)
Strand = Plus / Plus
Query: 169 gacttcatcttcggcaactcggcggcggtgttcca 203
|||||||||||||||||||||||||| ||| ||||
Sbjct: 373 gacttcatcttcggcaactcggcggccgtgctcca 407
>gb|BQ765469.1|BQ765469 EBro03_SQ007_C09_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ007_C09 5', mRNA sequence
Length = 417
Score = 50.1 bits (25), Expect = 8e-005
Identities = 49/57 (85%)
Strand = Plus / Plus
Query: 448 atgccctggaacggcgacttcggcatcaagacgctctactacgccgagtacaacaac 504
||||| |||| ||||||||||| ||| ||||||||||||||||||||| |||||
Sbjct: 71 atgccgtggagcggcgacttcgcgctcagcacgctctactacgccgagtacgacaac 127
>gb|AV914255.1|AV914255 AV914255 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags5g01 5', mRNA sequence
Length = 269
Score = 48.1 bits (24), Expect = 3e-004
Identities = 24/24 (100%)
Strand = Plus / Plus
Query: 33 gcaccaggcggtggcgctccgcgt 56
||||||||||||||||||||||||
Sbjct: 228 gcaccaggcggtggcgctccgcgt 251
>gb|AV914270.1|AV914270 AV914270 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags5h01 5', mRNA sequence
Length = 250
Score = 48.1 bits (24), Expect = 3e-004
Identities = 24/24 (100%)
Strand = Plus / Plus
Query: 33 gcaccaggcggtggcgctccgcgt 56
||||||||||||||||||||||||
Sbjct: 211 gcaccaggcggtggcgctccgcgt 234
>gb|BQ661505.1|BQ661505 HM03L14u HM Hordeum vulgare subsp. vulgare cDNA clone HM03L14
3-PRIME, mRNA sequence
Length = 298
Score = 46.1 bits (23), Expect = 0.001
Identities = 62/75 (82%)
Strand = Plus / Minus
Query: 448 atgccctggaacggcgacttcggcatcaagacgctctactacgccgagtacaacaaccgc 507
||||| |||| ||||||||||| || | ||||||||||||| ||||||| || | |
Sbjct: 293 atgccatggagcggcgacttcgcgctcgacacgctctactacggcgagtacggcagcgcc 234
Query: 508 ggccccggcgccggc 522
|||||||||||||||
Sbjct: 233 ggccccggcgccggc 219
>gb|CB859122.1|CB859122 HI09G17w HI Hordeum vulgare subsp. vulgare cDNA clone HI09G17
3-PRIME, mRNA sequence
Length = 565
Score = 46.1 bits (23), Expect = 0.001
Identities = 62/75 (82%)
Strand = Plus / Minus
Query: 448 atgccctggaacggcgacttcggcatcaagacgctctactacgccgagtacaacaaccgc 507
||||| |||| ||||||||||| || | ||||||||||||| ||||||| || | |
Sbjct: 292 atgccatggagcggcgacttcgcgctcgacacgctctactacggcgagtacggcagcgcc 233
Query: 508 ggccccggcgccggc 522
|||||||||||||||
Sbjct: 232 ggccccggcgccggc 218
>gb|CB859989.1|CB859989 HI12E03w HI Hordeum vulgare subsp. vulgare cDNA clone HI12E03
3-PRIME, mRNA sequence
Length = 521
Score = 46.1 bits (23), Expect = 0.001
Identities = 62/75 (82%)
Strand = Plus / Minus
Query: 448 atgccctggaacggcgacttcggcatcaagacgctctactacgccgagtacaacaaccgc 507
||||| |||| ||||||||||| || | ||||||||||||| ||||||| || | |
Sbjct: 293 atgccatggagcggcgacttcgcgctcgacacgctctactacggcgagtacggcagcgcc 234
Query: 508 ggccccggcgccggc 522
|||||||||||||||
Sbjct: 233 ggccccggcgccggc 219
>gb|CV062610.1|CV062610 BNEL80h9 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL80h9 5' similar to H.vulgare,
mRNA sequence
Length = 407
Score = 42.1 bits (21), Expect = 0.020
Identities = 21/21 (100%)
Strand = Plus / Minus
Query: 507 cggccccggcgccggcaccag 527
|||||||||||||||||||||
Sbjct: 329 cggccccggcgccggcaccag 309
>gb|BE422260.1|BE422260 HWM022cC.01r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM022cC.01, mRNA sequence
Length = 1239
Score = 38.2 bits (19), Expect = 0.32
Identities = 40/47 (85%)
Strand = Plus / Plus
Query: 478 acgctctactacgccgagtacaacaaccgcggccccggcgccggcac 524
||||||||||||| ||||||| | ||| || ||||||||||||||
Sbjct: 234 acgctctactacggcgagtaccagaacacgggacccggcgccggcac 280
>gb|BI960087.1|BI960087 HVSMEn0023C13f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0023C13f, mRNA sequence
Length = 708
Score = 38.2 bits (19), Expect = 0.32
Identities = 19/19 (100%)
Strand = Plus / Plus
Query: 120 gcacgcccggcgccagttc 138
|||||||||||||||||||
Sbjct: 573 gcacgcccggcgccagttc 591
>gb|BJ468665.1|BJ468665 BJ468665 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags39m02 3', mRNA sequence
Length = 606
Score = 38.2 bits (19), Expect = 0.32
Identities = 40/47 (85%)
Strand = Plus / Minus
Query: 478 acgctctactacgccgagtacaacaaccgcggccccggcgccggcac 524
||||||||||||| ||||||| | ||| || ||||||||||||||
Sbjct: 508 acgctctactacggcgagtaccagaacacgggacccggcgccggcac 462
>gb|BM374246.2|BM374246 EBpi03_SQ003_B10_R pistil, 4 DPA, no treatment, cv Optic, EBpi03
Hordeum vulgare subsp. vulgare cDNA clone
EBpi03_SQ003_B10 5', mRNA sequence
Length = 449
Score = 38.2 bits (19), Expect = 0.32
Identities = 40/47 (85%)
Strand = Plus / Plus
Query: 478 acgctctactacgccgagtacaacaaccgcggccccggcgccggcac 524
||||||||||||| ||||||| | ||| || ||||||||||||||
Sbjct: 95 acgctctactacggcgagtaccagaacacgggacccggcgccggcac 141
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 87,110
Number of Sequences: 312970
Number of extensions: 87110
Number of successful extensions: 24898
Number of sequences better than 0.5: 17
Number of HSP's better than 0.5 without gapping: 11
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 24860
Number of HSP's gapped (non-prelim): 30
length of query: 593
length of database: 175,134,539
effective HSP length: 19
effective length of query: 574
effective length of database: 169,188,109
effective search space: 97113974566
effective search space used: 97113974566
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)