BLASTN 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3183567.2.1
(681 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG415468.1|BG415468 HVSMEk0006J19f Hordeum vulgare testa... 256 8e-067
gb|BU994365.1|BU994365 HM06M18r HM Hordeum vulgare subsp. v... 198 2e-049
gb|BF256034.2|BF256034 HVSMEf0008I19f Hordeum vulgare seedl... 186 6e-046
gb|BI958231.1|BI958231 HVSMEn0013P22f Hordeum vulgare rachi... 107 5e-022
gb|BI960422.1|BI960422 HVSMEn0024I18f Hordeum vulgare rachi... 103 7e-021
gb|BQ660428.1|BQ660428 HI02H22u HI Hordeum vulgare subsp. v... 101 3e-020
gb|BQ664916.1|BQ664916 HX01G08w HX Hordeum vulgare subsp. v... 80 1e-013
gb|BG417280.2|BG417280 HVSMEk0017B12f Hordeum vulgare testa... 40 0.092
gb|AJ462887.1|AJ462887 AJ462887 S00002 Hordeum vulgare subs... 40 0.092
gb|BJ464879.1|BJ464879 BJ464879 K. Sato unpublished cDNA li... 40 0.092
gb|BQ659456.1|BQ659456 HE01G13u HE Hordeum vulgare subsp. v... 40 0.092
gb|BU986619.1|BU986619 HF12G18r HF Hordeum vulgare subsp. v... 40 0.092
gb|BU986660.1|BU986660 HF12I18r HF Hordeum vulgare subsp. v... 40 0.092
gb|CB876565.1|CB876565 HX11K19w HX Hordeum vulgare subsp. v... 40 0.092
gb|AV920222.1|AV920222 AV920222 K. Sato unpublished cDNA li... 38 0.37
>gb|BG415468.1|BG415468 HVSMEk0006J19f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0006J19f, mRNA sequence
Length = 666
Score = 256 bits (129), Expect = 8e-067
Identities = 249/289 (86%)
Strand = Plus / Minus
Query: 312 ggggttgaacggtgcctgctcggcgcaatcacggttgtcgccagagtgctcacggccgac 371
||||||||| || |||||||||||||| || ||||| ||| ||| |||| |||||||
Sbjct: 289 ggggttgaagggggcctgctcggcgcagtcgaggttgaggccggagcgctcgcggccgaa 230
Query: 372 gcccatgaggtcaaggtaatactggtagtgcgagattataacgttcatggcgtcgacgta 431
|||||||||||| ||||| ||||||||||| ||||| || |||||||| |||||| | |
Sbjct: 229 gcccatgaggtcgaggtagtactggtagtgggagatgatgacgttcatctcgtcgatgaa 170
Query: 432 tcccttgccgcagatcgactcgccgtagagtatgttcatggtggcgccgaacccaggcag 491
||| |||||||||| |||||||||||||| | |||||||||||||||||| || || ||
Sbjct: 169 gcccctgccgcagatggactcgccgtagaggaggttcatggtggcgccgaagcccgggag 110
Query: 492 gcgtttgctgagcgtgtcgttcttggtgggcttccagttgcccacgaaggcgtcgtgcgc 551
||| |||||||||||||||||||||||||||||||| ||| |||||| ||||||||
Sbjct: 109 gcgcttgctgagcgtgtcgttcttggtgggcttccacacccccgtgaaggcctcgtgcgc 50
Query: 552 cgacggctggctcttcttgatcggcgtcatccaccgccacatcgccgcc 600
||||||||| |||||||||| |||||||||||||||||||| ||||||
Sbjct: 49 cgacggctgcttcttcttgatgggcgtcatccaccgccacatggccgcc 1
>gb|BU994365.1|BU994365 HM06M18r HM Hordeum vulgare subsp. vulgare cDNA clone HM06M18
5-PRIME, mRNA sequence
Length = 597
Score = 198 bits (100), Expect = 2e-049
Identities = 187/216 (86%)
Strand = Plus / Plus
Query: 312 ggggttgaacggtgcctgctcggcgcaatcacggttgtcgccagagtgctcacggccgac 371
||||||||| || |||||||||||||| || ||||| ||| ||| |||| |||||||
Sbjct: 372 ggggttgaagggggcctgctcggcgcagtcgaggttgaggccggagcgctcgcggccgaa 431
Query: 372 gcccatgaggtcaaggtaatactggtagtgcgagattataacgttcatggcgtcgacgta 431
|||||||||||| ||||| ||||||||||| ||||| || |||||||| |||||| | |
Sbjct: 432 gcccatgaggtcgaggtagtactggtagtgggagatgatgacgttcatctcgtcgatgaa 491
Query: 432 tcccttgccgcagatcgactcgccgtagagtatgttcatggtggcgccgaacccaggcag 491
||| |||||||||| |||||||||||||| | |||||||||||||||||| || || ||
Sbjct: 492 gcccctgccgcagatggactcgccgtagaggaggttcatggtggcgccgaagcccgggag 551
Query: 492 gcgtttgctgagcgtgtcgttcttggtgggcttcca 527
||| ||||||||||||||||||||||||||||||||
Sbjct: 552 gcgcttgctgagcgtgtcgttcttggtgggcttcca 587
>gb|BF256034.2|BF256034 HVSMEf0008I19f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0008I19f, mRNA sequence
Length = 1033
Score = 186 bits (94), Expect = 6e-046
Identities = 160/182 (87%)
Strand = Plus / Minus
Query: 448 gactcgccgtagagtatgttcatggtggcgccgaacccaggcaggcgtttgctgagcgtg 507
||||||||| | || | ||||||||||||||| || || |||||||| ||||||||||||
Sbjct: 532 gactcgccgcacagcaggttcatggtggcgcccaagcccggcaggcgcttgctgagcgtg 473
Query: 508 tcgttcttggtgggcttccagttgcccacgaaggcgtcgtgcgccgacggctggctcttc 567
|||||||||||||||||||| ||| |||||| ||||||||||||||||| |||||
Sbjct: 472 tcgttcttggtgggcttccacacccccgtgaaggcctcgtgcgccgacggctgcttcttc 413
Query: 568 ttgatcggcgtcatccaccgccacatcgccgccatgaatgccagcgtcgcgttctgctcc 627
||||| |||||||||||||||||||| |||||| ||| |||||||||||||||||||||
Sbjct: 412 ttgatgggcgtcatccaccgccacatggccgccgcgaacgccagcgtcgcgttctgctcc 353
Query: 628 ag 629
||
Sbjct: 352 ag 351
>gb|BI958231.1|BI958231 HVSMEn0013P22f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0013P22f, mRNA sequence
Length = 914
Score = 107 bits (54), Expect = 5e-022
Identities = 83/93 (89%)
Strand = Plus / Minus
Query: 537 gaaggcgtcgtgcgccgacggctggctcttcttgatcggcgtcatccaccgccacatcgc 596
|||||| ||||||||||||||||| |||||||||| || ||||||||||||||||| ||
Sbjct: 445 gaaggcctcgtgcgccgacggctgcttcttcttgatgggggtcatccaccgccacatggc 386
Query: 597 cgccatgaatgccagcgtcgcgttctgctccag 629
|||| ||| |||||||||||||| ||||||||
Sbjct: 385 cgccgcgaacgccagcgtcgcgttntgctccag 353
>gb|BI960422.1|BI960422 HVSMEn0024I18f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0024I18f, mRNA sequence
Length = 683
Score = 103 bits (52), Expect = 7e-021
Identities = 118/140 (84%)
Strand = Plus / Minus
Query: 490 aggcgtttgctgagcgtgtcgttcttggtgggcttccagttgcccacgaaggcgtcgtgc 549
||||| |||||||||||||||||||||||||||| || || ||| || ||||||
Sbjct: 513 aggcgcttgctgagcgtgtcgttcttggtgggctcccccaccccagtgaacgcctcgtgc 454
Query: 550 gccgacggctggctcttcttgatcggcgtcatccaccgccacatcgccgccatgaatgcc 609
||| |||||| |||||||||| |||||||||| ||||||||| |||||| ||| |||
Sbjct: 453 cccgccggctgcttcttcttgatgggcgtcatcccccgccacatggccgccgcgaacgcc 394
Query: 610 agcgtcgcgttctgctccag 629
||||||||||||||||||||
Sbjct: 393 agcgtcgcgttctgctccag 374
>gb|BQ660428.1|BQ660428 HI02H22u HI Hordeum vulgare subsp. vulgare cDNA clone HI02H22
3-PRIME, mRNA sequence
Length = 488
Score = 101 bits (51), Expect = 3e-020
Identities = 120/143 (83%)
Strand = Plus / Plus
Query: 312 ggggttgaacggtgcctgctcggcgcaatcacggttgtcgccagagtgctcacggccgac 371
||||||||| || |||||||||||||| || ||||| ||| ||| |||| |||||||
Sbjct: 346 ggggttgaagggggcctgctcggcgcagtcgaggttgaggccggagcgctcgcggccgaa 405
Query: 372 gcccatgaggtcaaggtaatactggtagtgcgagattataacgttcatggcgtcgacgta 431
|||||||||||| ||||| ||||||||||| ||||| || |||||||| |||||| | |
Sbjct: 406 gcccatgaggtcgaggtagtactggtagtgggagatgatgacgttcatctcgtcgatgaa 465
Query: 432 tcccttgccgcagatcgactcgc 454
||| |||||||||| |||||||
Sbjct: 466 gcccctgccgcagatggactcgc 488
>gb|BQ664916.1|BQ664916 HX01G08w HX Hordeum vulgare subsp. vulgare cDNA clone HX01G08
3-PRIME, mRNA sequence
Length = 514
Score = 79.8 bits (40), Expect = 1e-013
Identities = 82/96 (85%)
Strand = Plus / Plus
Query: 312 ggggttgaacggtgcctgctcggcgcaatcacggttgtcgccagagtgctcacggccgac 371
||||||||| || |||||||||||||| || ||||| ||| ||| |||| |||||||
Sbjct: 416 ggggttgaagggggcctgctcggcgcagtcgaggttgaggccggagcgctcgcggccgaa 475
Query: 372 gcccatgaggtcaaggtaatactggtagtgcgagat 407
|||||||||||| ||||| ||||||||||| |||||
Sbjct: 476 gcccatgaggtcgaggtagtactggtagtgggagat 511
>gb|BG417280.2|BG417280 HVSMEk0017B12f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0017B12f, mRNA sequence
Length = 886
Score = 40.1 bits (20), Expect = 0.092
Identities = 74/92 (80%)
Strand = Plus / Minus
Query: 505 gtgtcgttcttggtgggcttccagttgcccacgaaggcgtcgtgcgccgacggctggctc 564
||||| |||||||| || ||||||||||| || ||||| || || || || ||||| ||
Sbjct: 413 gtgtctttcttggttggtttccagttgccaacaaaggcatcatgggctgatggctgcttc 354
Query: 565 ttcttgatcggcgtcatccaccgccacatcgc 596
|||| || || |||||||||| ||| |||||
Sbjct: 353 ctcttcattggagtcatccacctccagatcgc 322
>gb|AJ462887.1|AJ462887 AJ462887 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200008A03F1, mRNA sequence
Length = 420
Score = 40.1 bits (20), Expect = 0.092
Identities = 74/92 (80%)
Strand = Plus / Minus
Query: 505 gtgtcgttcttggtgggcttccagttgcccacgaaggcgtcgtgcgccgacggctggctc 564
||||| |||||||| || ||||||||||| || ||||| || || || || ||||| ||
Sbjct: 187 gtgtctttcttggttggtttccagttgccaacaaaggcatcatgggctgatggctgcttc 128
Query: 565 ttcttgatcggcgtcatccaccgccacatcgc 596
|||| || || |||||||||| ||| |||||
Sbjct: 127 ctcttcattggagtcatccacctccagatcgc 96
>gb|BJ464879.1|BJ464879 BJ464879 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags36g21 5', mRNA sequence
Length = 667
Score = 40.1 bits (20), Expect = 0.092
Identities = 74/92 (80%)
Strand = Plus / Minus
Query: 505 gtgtcgttcttggtgggcttccagttgcccacgaaggcgtcgtgcgccgacggctggctc 564
||||| |||||||| || ||||||||||| || ||||| || || || || ||||| ||
Sbjct: 382 gtgtctttcttggttggtttccagttgccaacaaaggcatcatgggctgatggctgcttc 323
Query: 565 ttcttgatcggcgtcatccaccgccacatcgc 596
|||| || || |||||||||| ||| |||||
Sbjct: 322 ctcttcattggagtcatccacctccagatcgc 291
>gb|BQ659456.1|BQ659456 HE01G13u HE Hordeum vulgare subsp. vulgare cDNA clone HE01G13
3-PRIME, mRNA sequence
Length = 550
Score = 40.1 bits (20), Expect = 0.092
Identities = 74/92 (80%)
Strand = Plus / Plus
Query: 505 gtgtcgttcttggtgggcttccagttgcccacgaaggcgtcgtgcgccgacggctggctc 564
||||| |||||||| || ||||||||||| || ||||| || || || || ||||| ||
Sbjct: 375 gtgtctttcttggttggtttccagttgccaacaaaggcatcatgggctgatggctgcttc 434
Query: 565 ttcttgatcggcgtcatccaccgccacatcgc 596
|||| || || |||||||||| ||| |||||
Sbjct: 435 ctcttcattggagtcatccacctccagatcgc 466
>gb|BU986619.1|BU986619 HF12G18r HF Hordeum vulgare subsp. vulgare cDNA clone HF12G18
5-PRIME, mRNA sequence
Length = 606
Score = 40.1 bits (20), Expect = 0.092
Identities = 74/92 (80%)
Strand = Plus / Minus
Query: 505 gtgtcgttcttggtgggcttccagttgcccacgaaggcgtcgtgcgccgacggctggctc 564
||||| |||||||| || ||||||||||| || ||||| || || || || ||||| ||
Sbjct: 414 gtgtctttcttggttggtttccagttgccaacaaaggcatcatgggctgatggctgcttc 355
Query: 565 ttcttgatcggcgtcatccaccgccacatcgc 596
|||| || || |||||||||| ||| |||||
Sbjct: 354 ctcttcattggagtcatccacctccagatcgc 323
>gb|BU986660.1|BU986660 HF12I18r HF Hordeum vulgare subsp. vulgare cDNA clone HF12I18
5-PRIME, mRNA sequence
Length = 636
Score = 40.1 bits (20), Expect = 0.092
Identities = 74/92 (80%)
Strand = Plus / Minus
Query: 505 gtgtcgttcttggtgggcttccagttgcccacgaaggcgtcgtgcgccgacggctggctc 564
||||| |||||||| || ||||||||||| || ||||| || || || || ||||| ||
Sbjct: 414 gtgtctttcttggttggtttccagttgccaacaaaggcatcatgggctgatggctgcttc 355
Query: 565 ttcttgatcggcgtcatccaccgccacatcgc 596
|||| || || |||||||||| ||| |||||
Sbjct: 354 ctcttcattggagtcatccacctccagatcgc 323
>gb|CB876565.1|CB876565 HX11K19w HX Hordeum vulgare subsp. vulgare cDNA clone HX11K19
3-PRIME, mRNA sequence
Length = 618
Score = 40.1 bits (20), Expect = 0.092
Identities = 74/92 (80%)
Strand = Plus / Plus
Query: 505 gtgtcgttcttggtgggcttccagttgcccacgaaggcgtcgtgcgccgacggctggctc 564
||||| |||||||| || ||||||||||| || ||||| || || || || ||||| ||
Sbjct: 402 gtgtctttcttggttggtttccagttgccaacaaaggcatcatgggctgatggctgcttc 461
Query: 565 ttcttgatcggcgtcatccaccgccacatcgc 596
|||| || || |||||||||| ||| |||||
Sbjct: 462 ctcttcattggagtcatccacctccagatcgc 493
>gb|AV920222.1|AV920222 AV920222 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags9n02 3', mRNA sequence
Length = 393
Score = 38.2 bits (19), Expect = 0.37
Identities = 25/27 (92%)
Strand = Plus / Plus
Query: 312 ggggttgaacggtgcctgctcggcgca 338
||||||||| || ||||||||||||||
Sbjct: 356 ggggttgaagggggcctgctcggcgca 382
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 77,312
Number of Sequences: 312970
Number of extensions: 77312
Number of successful extensions: 18678
Number of sequences better than 0.5: 15
Number of HSP's better than 0.5 without gapping: 15
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 18659
Number of HSP's gapped (non-prelim): 17
length of query: 681
length of database: 175,134,539
effective HSP length: 19
effective length of query: 662
effective length of database: 169,188,109
effective search space: 112002528158
effective search space used: 112002528158
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 19 (38.2 bits)